Abstract—The aim of this study was to investigate the protective effect of exogenous peroxiredoxins with ischemia–reperfusion of an isolated kidney. The study was carried out using a model of isolated rat kidney perfusion ex vivo. A recombinant peroxiredoxin 6 was injected directly in the perfusion buffer. The high-molecular-weight dye Blue Dextran 2000 and urea were added to the perfusion buffer to determine the functionality of an isolated kidney. It was demonstrated that exogenous peroxiredoxin 6 in the cortical layer of an isolated kidney was localized in the vessels of renal glomeruli; in the medulla it was found in microvessels surrounding the thin tubules. The use of peroxiredoxin 6 decreases the degree of damage of nephron structures by two times compared with the damage in the control, which provides the preservation of ultrafiltration processes. A decrease in glomerular damage leads to a decrease in the content of Blue Dextran by two times compared with the damage in the control at the end of the perfusion period. A decrease in the damage of tubular structures indicates the active urea transport during perfusion. Thus, the inclusion of peroxiredoxin 6 in the perfusion buffer mediated a decrease in the damage of nephron structures and maintenance of the multifunctional state of renal glomeruli and tubules.