Coral trout Plectropomus leopardus is a high-economical value of marine fish commodity. So, the larviculture for this species should be performed in hatchery scale for their sustainability. One of the important thing in larval stage is hormonal mechanisms. Thyroid hormone is known plays an important role in the metamorphosis of marine fish larvae. The source of that hormone is assumed derived from zooplankton as their live feeds. The live feeds commonly used for marine fish larvae in hatchery are zooplankton copepods and rotifers. The purpose of this research to determine the influence of feeding copepods and rotifers on thyroid hormone profile activity, consisted of triiodothyronine (T3) and thyroxine (T4), related to the metamorphoses of coral trout larvae. The research was conducted using three feeding schemes: copepods (A), copepods-rotifers (B), and rotifers (C). It was done for 50 Days After Hatching (DAH). The results showed that larvae from treatment (A) and (B) achieved the highest level of T3 and T4 earlier at 30 DAH, which indicated that both of those larvae have entered the metamorphosis stage earlier. On the other hand, larvae from treatment (C) achieved it and entered the metamorphosis stage later at 40 DAH. In addition, iodine levels in the copepods were significantly higher than those of in rotifers (P < 0.05). Based on this research, live feeds copepods and rotifers have effect on thyroid hormone activity. Copepods have better potential in accelerating the metamorphosis achievement of coral trout larvae with the fact that they have higher iodine content.