Activated Sludge Treatment System Research Articles

Life cycle assessment of anaerobic-anoxic-oxic and extended aeration of the activated sludge treatment system for urban wastewater treatment: a case study in Iran

Life cycle assessment of anaerobic-anoxic-oxic and extended aeration of the activated sludge treatment system for urban wastewater treatment: a case study in Iran

Optimal Controllers for the Operation of Activated Sludge Treatment Systems

Objective: Application of Optimal Control Theory to the activated sludge process in order to regulate the removal of the carbonaceous part of the organic matter. Theoretical benchmark: Dynamic models of activated sludge processes are capable of enabling the development of innovative control systems. The Optimal Control Theory, aimed at optimizing the performance of dynamic systems, allows the establishment of control systems that enhance efficiency in adapting to changes in operating conditions, ensuring satisfactory process performance. Method: The proposed control systems were established from the application of Optimal Control Theory, considering the use of the dynamic model of the activated sludge process presented by International Association on Water Pollution Research and Control. Computer simulations were used to assess the performance of the control systems proposed. Results and conclusion: The proposed control systems were capable of curbing substantially the oscillations in the concentrations of biomass, of particulate matter produced by the degradation of biomass and those of the inert particulate substrate. The reductions in the oscillations of the slowly biodegradable part of the influent were relatively smaller. The controller was quite effective in leading the wastewater treatment system sooner to the new equilibrium conditions after the imposition of the disturbances in raw effluent. Implications of research: Development of control systems for activated sludge processes to regulate the removal of the carbonaceous fraction from organic matter. Originality/value: The establishment of optimal control systems for activated sludge processes can enable greater efficiency and operational capacity, especially in industrial facilities or densely populated urban settlements, where such wastewater treatment systems are most commonly used.

Differential Impact of the Biodegradation Sunflower Oil, Particulate Substrate, Caused by the Presence of Saccharose, Soluble Substrate, on Activated Sludge Treatment

This research studies the biodegradation of sunflower-type vegetative oil in two proposed activated sludge systems, the first one to biologically treat an influent containing only vegetative oil and the second one to treat a mixture of vegetable oil plus saccharose. The purpose of these analyses is to evaluate the differential impact caused by the soluble substrate saccharose on the removal of vegetative oil. Vegetative oil biodegradation in both systems was studied and quantified via integral mass balance, and relevant operating parameters were monitored. This experimentation based on the mass balance estimation of biodegraded vegetative oil serves as a reference to understand the effect of soluble substrates present in mixed wastewater on oil biodegradation. Information was generated on the performance of the two activated sludge treatment systems. Both influents were pre-stirred before they entered the bench-scale activated sludge plants. The working range for sunflower oil concentration was 120 to 520 mg/L for the influent with sunflower oil and 180 to 750 mg/L for the influent with sunflower oil and saccharose. Biodegradation was in the order of 56 to 72% and 47 to 67%, respectively. The removal of sunflower oil in biodegradation and flotation was in the order of 90% in both scenarios.

A novel insight into energy conservation and efficiency enhancement in wastewater treatment process: Low-frequency mechanical vibration induced

One important way to break the bottleneck of energy conservation and consumption reduction in sewage treatment process is to attenuate the mass transfer resistance, which is thought as one of key factors to determine the efficiency of activated sludge treatment system. Thus, in this study, two independent Anacrobic/Oxic reactors were operated under low dissolved oxygen (DO) condition to evaluate the effects of low-frequency mechanical vibrations on the removal efficiency of pollutants. Results indicate that 40 Hz low-frequency mechanical vibration could greatly strengthen the efficiency of pollutants removal. Microelectrode-mass transfer efficiency test platform revealed that 40 Hz low-frequency mechanical vibration could increase the oxygen transfer coefficient from gas to liquid by approximately 145.19 %, and oxygen diffusion coefficient from liquid to solid by 75.2 %. Fractal dimension of flocs, particle size and zeta potential analyses jointly suggested that low-frequency mechanical vibrations treatment can form more adsorption points and mass transfer channels to enhance mass transfer efficiency of oxygen in floc. In addition, low-frequency mechanical vibrations treatment further stimulated the biological activities associated with nitrogen metabolism (i.e., specific metabolism rate, metabolism enzyme activities), but slightly alerted the overall microbial community. The findings in this study suggest that low-frequency mechanical vibrations could effectively accelerate the pollutants removal under low-DO condition by enhancing oxygen transfer coefficient and stimulating the associated biological activities. Collectively, this study provides a new technical strategy for efficiency enhancement and energy conservation in the global sewage treatment industry.

Persistence of surrogates for high consequence viral and bacterial pathogens in a pilot-scale activated sludge treatment system.

The persistence of high consequence public health pathogens in a wastewater treatment system can significantly impact worker safety, as well as the public and downstream water bodies, particularly if the system is forced to shut down the treatment processes. This study utilizes organism viability to compare the persistence of three pathogen surrogates in wastewater using a pilot-scale activated sludge treatment (AST) system, operated to mimic treatment processes of large-scale plants. Bacillus globigii spores, surrogate for Bacillus anthracis, persisted in the AST system for at least a 50-day observation period leading to a possible steady condition far beyond the solid retention time for sludge particles. MS2 bacteriophage, surrogate for Poliovirus and other non-enveloped enteric viruses, was observed for up to 35 days after introduction, which largely and expectedly correlated to the measured solid retention time. Phi-6 bacteriophage, a surrogate for Ebola virus and other enveloped viruses, was detected for no more than 4 days after introduction, even though the AST system was operated to provide three times slower solids removal than for the other surrogates. This suggests Phi-6 is subject to inactivation under AST conditions rather than physical removal. These results may suggest similar persistence for the surrogated pathogens, leading to appropriate consequence management actions.

Antibiotic adsorption by natural and modified clay minerals as designer adsorbents for wastewater treatment: A comprehensive review

Increased antibiotic use worldwide has become a major concern because of their health and environmental impacts. Since most antibiotic residues can hardly be removed from wastewater using conventional treatments, alternative methods receive great attention. Adsorption is one of the most efficient and cost-effective treatment methods for antibiotics. Among the adsorbents, clay minerals have garnered increasing attention due to their unique properties including availability, high specific surface area, low cost, cation exchange capacity, and good removal efficiency. This paper reviews the recent progress made in the use of natural and modified clay minerals for the removal of antibiotics from water. First, the sources, occurrence, removal and health effects of the antibiotics commonly encountered in water bodies are described. Antibiotic concentration levels and average removal efficiencies measured in conventional activated sludge treatment systems worldwide are also provided to better address the problem. Second, the review explores the characteristics of clay minerals as adsorbent of antibiotics and the factors affecting the adsorption. The review identifies and discusses the future trends and strategies used to increase the adsorption capacity of clay minerals by modification and combination techniques (intercalation of novel functional groups such as organocations, biopolymers and metal pillared-clay minerals, combination with biochar or thermal activation). The quantitative comparisons of clay minerals' ability for antibiotic removal are given. Some natural clay minerals have good removal potential for antibiotics, with maximum adsorption capacities over 100mg/g. For most other adsorbents, surface modifications and combination techniques resulted in improved adsorption properties (including higher surface area, enhanced adsorption capacity, increased stability and mechanical strength). Finally, the application of these adsorbents at pilot scale, using real wastewater samples, their reuse, economic analysis and life cycle assessment are other issues that have been considered.

Minimizing Foaming and Bulking in Activated Sludge with Bacteriophage Treatment: A Review of Mathematical Modeling

The interest in the ability of phages to control bacterial populations has extended from medical applications into the fields of agriculture, aquaculture, and the food industry. In particular, several authors have proposed using bacteriophages as an alternative method to control foaming and bulking in wastewater treatment. This strategy has shown successful results at the laboratory scale. However, this technology is still in development, and there are several challenges to overcome before bacteriophages can be widely used to control foaming and bulking in pilot or larger-scale treatment plants. Several models of the infection mechanisms in individual bacteria–phage pairs have been reported, i.e., for controlled systems with only one bacterium species in the presence of one phage species. However, activated sludge treatment systems largely differ from this situation, which opens a large horizon for future research. Mathematical models will play a key role in this development process, and this review offers an overview of the proposed models: their applications, potential, and challenges. A particular focus is placed on the model properties, such as parameter identifiability and states’ observability, which are essential for process prediction, monitoring, or dynamic optimization.

Applying novel methods in conventional activated sludge plants to treat low-strength wastewater

Conventional activated sludge system is confidently widely used for biological treatment plants of municipal wastewater but suffering from operation problems that affect their efficiencies and effluent qualities, especially when treating low-strength wastewater with increasing incoming flow. The objective of this study is to evaluate and compare the novel methods used in upgrading conventional activated sludge treatment systems receiving low-strength wastewater to generate good effluent quality. GPS-X Simulator V 8.0 was used for model calibration and plant performance prediction. The calibrated GPS-X model proved that eliminating primary settling from the treatment process does not affect BOD5 and COD removal, while TSS removal is decreased, and NH4-N removal is increased. Increasing the return activated sludge flow from 50 to 150% of influent flow does not affect conventional activated sludge process, while the change of waste activated flow had a vital effect on process performance. The presence of an anoxic zone in conventional activated sludge processes treating low-strength wastewater has no significant impact on plant performance. Also, the model outputs proved that adding filling media to the aeration tank was able to handle an increase of influent flow and a stable performance of BOD5, and NH4-N removal was observed.

Organic and nutrient removal from pulp and paper industry wastewater by extended aeration activated sludge system

Pulp and paper industries are critical to a country’s economic growth. The type of raw material used and the pulping process determine the quality and quantity of wastewater generated. However, the generated wastewater with a dark colour comprises a high concentration of suspended solids, organic content, chemical oxygen demand (COD), volatile organic compounds, and a variety of other impurities. Therefore, in this study, a bench scale activated sludge treatment system was set up using a reactor consisting of an aeration tank with 5000 mg/L initial biomass and a clarifier chamber for the biomass to settle. The reactor was run few weeks with real domestic wastewater as the influent for 3 weeks to acclimatize the sludge inside the reactor. The reactor was then fed with the influent mixture of 20% industrial pulp wastewater and 80% domestic wastewater. Organic and nutrient parameter concentrations are tested from the influent and effluent sample throughout the study duration and recorded for data analysis. The removal of COD and TSS are at 83% and 90% respectively while the averaged BOD value of the treated wastewater is at 74.6%. The conclusion of this project is that the bench scale EAAS is able to treat BOD and TSS according to standard. However, a modification may be required to increase the efficiency of removing COD to meet the requirement standards. This modification could be either by using a biocarrier or an activated carbon to further enhance the treatment efficiency even at higher wastewater concentration.

Promotion of full-scale constructed wetlands in the wine sector: Comparison of greenhouse gas emissions with activated sludge systems

The aim of this study was to quantify and compare greenhouse gas (GHG) (i.e. carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4)) emissions from two full-scale winery wastewater and sludge treatment systems (i.e. constructed wetlands (CWs) and activated sludge system) located in Galicia (Spain). GHG fluxes were measured using the static chamber method in combination with an on-site Fourier transform infrared spectroscopy (FTIR) gas analyser in the CWs system. These on-site innovative techniques proved to be very accurate and reliable. In the activated sludge treatment systems, the floating chamber method in combination with the FTIR gas analyser was used. Measurements were carried out during the vintage season, when winery wastewater has the highest flow and loads, and the rest of the year. Emission rates of CO2, N2O and CH4 in the CWs units (i.e. vertical flow, horizontal subsurface flow and sludge treatment wetlands) ranged from 1.35E+02 to 7.54E+04, 1.70E-01 to 3.09E+01 and − 3.05E+01 to 1.79E+03 mg m−2 day−1, respectively. In the case of the activated sludge units (i.e. reactor, secondary settler and sludge storage tank) emission rates of CO2, N2O and CH4 ranged from 1.56E+04 to 1.43E+05, 1.13E+01 to 4.75E+01 and 2.52E+01 to 1.01E+03 mg m−2 day−1, respectively. Seasonally, daily and instantaneous variability in emissions as well as spatial variability was found. Comparing CWs with the activated sludge system, surface emission rates were lower in the CWs system in both seasons considered. Results highlighted that CWs are suitable technologies that can help to reduce GHG emissions associated with winery wastewater treatment.

Effect of pH on nutrient removal and crop production of hydroponic systems treating brewery effluent

Abstract The use of a crop to remove nutrients from brewery effluent and the influence of pH on these removal rates was evaluated. Cabbage (Brassica oleracea) was grown in recirculating hydroponic systems fed with post-anaerobically digested brewery effluent (BE) either subject to pH adjustment (6.5–7.0) or unaltered pH (8.0–8.5). These were compared with cabbages grown in water only and in a inorganic fertiliser nutrient solution (NS). Hydroponic systems fed with pH adjusted BE removed significantly more nitrogen and phosphorus than systems fed with pH unadjusted BE (p < 0.05). The final weight of cabbages from the pH adjusted BE systems were 6.7 times greater than cabbages from the pH unadjusted BE systems, whereas pH adjustment had no influence on cabbage weight in the water-only and NS treatments. Anaerobically digested BE that is not pH adjusted is not a suitable water and nutrient source for the hydroponic production of cabbages. However, pH adjustment of BE renders it more suitable for hydroponic crop production with hydroponic systems decreasing dissolved inorganic nitrogen, ammonium, phosphate and chemical oxygen demand concentrations by 72.8, 31.8, 98.5 and 51.0%, respectively. Hydroponic systems can be used to treat post-anaerobically digested BE to a similar standard obtained by conventional activated sludge treatment system.

Identifying the Effect of Non-Ideal Mixing on a Pre-Denitrification Activated Sludge System Performance through Model-Based Simulations

Effectiveness of a pre-denitrification activated sludge treatment system is governed by the kinetics of the biological reactions, and the hydrodynamic mixing behavior in the reactors. Achieving good mixing conditions within a reactor not only enhances the transfer of reactants but also ensures homogeneous environmental conditions throughout the vessel when required, allowing for an effective usage of the reactor’s total volume, leading to optimized, low-cost operation. In this work, a pre-denitrification activated sludge system performance with regards to the biological treatment of organic carbon and nitrogen was investigated, under two scenarios for non-ideal mixing in the anoxic reactor. The system performance is simulated based upon the Activated Sludge Model 1 model’s biological reactions, and combining two non-ideal mixing two-parameter models: CSTR with bypass and dead volume, and two CSTRs with exchange. Performance discrepancies were then identified in the presence of non-ideal mixing. The system’s performance was found to be more susceptible to the presence of a dead volume/bypass scenario compared to the two CSTRs with material exchange scenario. Under non-ideal mixing conditions, effluent concentrations of Total Kjeldahl Nitrogen, organic carbon increased marginally, while effluent concentration of nitrate increased significantly. Similarly, the waste stream concentrations of Total Kjeldahl Nitrogen and organic carbon increased significantly as a result of an increase in the concentration of the heterotrophic biomass. The outcome of this study provides an insight when troubleshooting the operation of pre-denitrification activated sludge systems for non-ideal mixing conditions.

Improving Substrate Consumption and Decrease of Growth Yield in Aerobic Cultures of Pseudomonas denitrificans By Applying Low Voltages in Bioelectric Systems.

It is well known that activated sludge treatment systems generate a lot of surplus sludge having environmental and economic impacts. Although several approaches have been proposed for the treatment/reuse of the excess of sludge, there are few studies focused on decreasing the biomass yield without affecting the metabolic activity. This work reports the effect of low magnitude electrical fields (0.07 to 0.2 V/cm) on the growth yield of a pure strain of Pseudomonas denitrificans (used as model microorganism). Cell potentials between 0.2 and 0.57 V were measured during 24 h to the aerobic culture; biomass production and substrate consumption were evaluated at regular intervals. Results indicated that the substrate (lactate) consumption efficiency increased with the applied potential, up to 100%, while the yield diminished 31% (0.34 g biomass/g lactate consumed) at 0.7 V vs. NHE. Bioenergetics showed that the fraction of electron equivalents toward biomass synthesis decreased from 0.68 (when no potential was applied) to 0.47 at 0.57 V, pointing out the redirection of the energy flow toward maintenance to cope with the stress caused by the imposed voltage. Therefore, the electrical stimulus could be used as control of biomass growth in aerobic wastewater treatment lines.

Importance of nutrient availability for soluble microbial products formation during a famine period of activated sludge: Evidence from multiple analyses

Much remains unknown about compositional variations in soluble microbial products (SMP) with the shift of the substrate condition from a feast to a famine phase in biological treatment systems. This study demonstrated that the formation of SMP could be suppressed by up to 75% during the famine phase with the addition of essential nutrients. In contrast, presence of electron acceptor did not play any significant role during the stress condition, showing the similar amounts of SMP (r = 0.98, p < 0.05) formation between the bioreactors supplied with air and N2. The SMP formed in the famine phase was more bio-refractory in the famine versus the feast phase with a linear correlation shown between the production and their aromatic structures in the composition (R2 > 0.95). The fluorescence excitation–emission matrix coupled with parallel factor analysis (EEM-PARAFAC) revealed the presence of four different fluorescent components, including two protein-like (C1 and C4), fulvic-like (C2), and humic-like (C3) components, in the SMP and bEPS formed at different conditions. Both C1 and C4 showed increasing trends (R2 > 0.95) with the length of starvation in the bioreactors without essential nutrients. Nutrient availability was found to be a key factor to quench the production of large-sized biopolymers. This study provides a wealth of information on operation conditions of activated sludge treatment systems to minimize large sized SMP molecules (particularly proteins), which typically exert many environmental concerns to effluent organic matter quality.

Ultrasound-induced settleability and membrane filterability of activated sludge treating milk processing wastewater

In practice, established operation of activated sludge treatment system with high biomass concentration is restricted because of difficulty in settling the sludge in clarifiers. Membrane bioreactor is a modified form of activated sludge, and despite the superior advantages, its widespread application is restricted by the membrane fouling. Therefore, in this research, to improve the sludge sedimentation at high biomass concentration in the activated sludge system, high-frequency ultrasound (1.7 MHz) was exerted in clarifier of activated sludge system and its performance was compared with a bioreactor followed by an antifouling ultrafiltration membrane. The antifouling ability of NH2-functionalized multiwall carbon nanotubes (NH2-MWCNTs)-modified nanocomposite UF membrane during filtration of mixed liquor-activated sludge was investigated. Hydraulic retention time (HRT) (8–44 h) and mixed liquor suspended solids (MLSS) (6000–14,000 mg/L) were chosen as the operating variables to analyze the process. The biological process showed high COD removal efficiency throughout the experiments (> 96%). The membrane and high-frequency ultrasound had no effect on the system performance in terms of COD, TKN, TN, TP removal. However, the HRT and MLSS indicated an increasing impact on flux. The effect of ultrasound on the sludge properties, i.e., sludge volume index, sludge settling velocity and height of sludge at high MLSS concentration (14,000 mg/L), was not considerable. It was concluded at MLSS of below 10,000 mg/L, about 40% water recovery was obtained. As a result, activated sludge equipped with ultrasound showed a promising performance; however, its industrial development needs further examinations to attain the design criteria.Graphical

Sources, mechanisms, and fate of steroid estrogens in wastewater treatment plants: a mini review

Steroid estrogens, such as estrone (E1), 17β-estradiol (E2), estriol (E3), and 17α-ethinylestradiol (EE2), are natural and synthetic hormones released into the environment through incomplete sewage discharge. This review focuses on the sources of steroid estrogens in wastewater treatment plants (WWTPs). The mechanisms and fate of steroid estrogens throughout the entire wastewater treatment system are also discussed, and relevant information on regulatory aspects is given. Municipal, pharmaceutical industry, and hospitals are the main sources of steroid estrogens that enter WWTPs. A typical WWTP comprises primary, secondary, and tertiary treatment units. Sorption and biodegradation are the main mechanisms for removal of steroid estrogens from WWTPs. The fate of steroid estrogens in WWTPs depends on the types of wastewater treatment systems. Steroid estrogens in the primary treatment unit are removed by sorption onto primary sludge, followed by sorption onto micro-flocs and biodegradation by microbes in the secondary treatment unit. Tertiary treatment employs nitrification, chlorination, or UV disinfection to improve the quality of the secondary effluent. Activated sludge treatment systems for steroid estrogens exhibit a removal efficiency of up to 100%, which is higher than that of the trickling filter treatment system (up to 75%). Moreover, the removal efficiency of advance treatment systems exceeds 90%. Regulatory aspects related to steroid estrogens are established, especially in the European Union. Japan is the only Asian country that implements a screening program and is actively involved in endocrine disruptor testing and assessment. This review improves our understanding of steroid estrogens in WWTPs, proposes main areas to be improved, and provides current knowledge on steroid estrogens in WWTPs for sustainable development.

Microfaunal community in horizontal constructed wetlands with different design configurations

In comparison with conventional activated sludge treatment systems, for which a large body of research has been carried out on their microfauna and their role in bacteria and pollutant removal, only a few studies have focused on microfaunal communities inhabiting constructed wetlands (CWs). The aim of this study was to evaluate the microfaunal communities of horizontal CWs with differing design configurations in order to determine those design factors affecting their abundance and community structure and to discover their role in bacteria removal. Total bacteria, ciliates, amoebae and metazoa were counted in the effluents of an experimental plant combining the most common design configurations of CWs. Three different hydraulic designs (hydroponic, free water surface—FWS and subsurface flow—SSF), presence vs. absence of vegetation, two plant species (Typha angustifolia vs. Phragmites australis) and two organic loading rates were compared. SSF and vegetation favoured bacteria removal whereas abundance of protozoa and diversity of metazoa was greater in FWS-planted wetlands. Microfauna community structure and bacterial removal were clearly affected by vegetation and flow type, although no significant relationships were observed between microfauna and bacteria abundance at the outflow. Therefore, other mechanisms such as filtration, sedimentation or adsorption, seem to be more important than predation in removing bacteria from constructed wetlands.

シャットダウン時における活性汚泥養生運転方法と臭気対策

シャットダウン時における活性汚泥養生運転方法と臭気対策

Understanding the distribution, degradation and fate of organophosphate esters in an advanced municipal sewage treatment plant based on mass flow and mass balance analysis

Although organophosphate esters (OPEs) in the ambient environment are from sewage treatment plants due to the discharge of effluent and application of sludge, the distribution, degradation and fate of OPEs in advanced municipal sewage treatment plants remain unclear. This work focused on the use of mass flow and mass balance analysis to understand the behaviors and fate of 14 OPEs in an advanced municipal sewage treatment plant. OPEs were detected in all sewage water and sludge samples with total OPEs (ΣOPEs) concentrations of 1399±263ng/L in raw sewage aqueous phase, 833±175ng/L in tertiary effluent aqueous phase, and 315±89ng/g dry weight in dewatered sludge. The dissolved concentrations of ΣOPEs significantly decreased during biological treatment, whereas negligible decrease was observed in mechanical and physical-chemical treatments. For individual OPE, the chlorinated tris(2-chloroethyl) phosphate (TCEP) and tris(2-chloroisopropyl) phosphate (TCPP) did not decrease but increased during both biological treatment and physical-chemical treatment. Mass flow analysis indicated the total removal efficiency of ΣOPEs in aqueous phase was 40.5%, and the polarity-specific removal efficiencies for individual OPE were positively related to their solid-water partition coefficients (Kd). Furthermore, mass balance results showed that 53.1% and 6.3% of the initial OPE mass flow were eventually transferred to the effluents and dewatered sludge, respectively, while the remaining 39.9% and 0.7% were lost due to biodegradation and physical-chemical treatment, respectively. It was indicated that the activated sludge treatment system with anaerobic/anoxic/aerobic bioreactors was a major factor in the removal of OPEs from the raw sewage, while transfer to dewatered sludge governed by hydrophobic interactions was limited during the sewage treatment. Meanwhile, the degradation difference of OPEs in activated sludge treatment was more related with their molecular structure over their hydrophobicity.

Response to shock load of engineered nanoparticles in an activated sludge treatment system: Insight into microbial community succession.

The environmental impacts of the use of engineered nanoparticles (NPs) remain unclear and have attracted increasing concern worldwide. Considering that NPs eventually end up in wastewater treatment systems, the potential impact of ZnO and TiO2 NPs on the activated sludge was investigated using laboratory-scale sequencing batch reactors (SBRs). Short-term (24 h) exposure to 1, 10 and 100 mg/L shock loads of NPs reduced the oxygen uptake rate of the activated sludge by 3.55%-12.51% compared with the controls. In our experiment, the toxicities of TiO2 NPs were higher than those of ZnO NPs as reflected in the inhibition of oxygen utilization in the activated sludge. However, both the short-term (24 h) and long-term (21 days) exposure to ZnO and TiO2 NPs did not adversely affect the pollutant removal of the SBRs. Furthermore, the polymerase chain reaction-denaturing gel gradient electrophoresis revealed that the microbial community did not significantly vary after the short-term exposure (24 h) to 1, 10 and 100 mg/L shock loads of NPs; however, the cluster analysis in our experiment revealed that the slight difference caused by the NPs largely depended on exposure time rather than on NP type and NP concentration. The long-term exposure (13 days) to 10 mg/L shock load of ZnO or TiO2 NPs caused no substantial microbial community shifts in the activated sludge. The microbial diversity also showed no significant change when exposed to NPs as revealed by the Shannon-Wiener index.