Action Of Malathion Research Articles

ПРОЯВИ ОКИСНОГО СТРЕСУ ТА МЕТАБОЛІЧНИХ ПОРУШЕНЬ У DANIO RERIO ЗА ДІЇ ФОСФООРГАНІЧНИХ ПЕСТИЦИДІВ

The aim of this study was to analyze the effects of roundup and malathion in environmentally relevant and subtoxic concentrations on the metabolic processes of zebrafish Danio rerio. Pesticide toxicity was assessed by changes in the activity of enzymes catalase, succinate dehydrogenase, glutathione transferase, glutathione reductase, protein phosphatase and caspase 3.
 Roundup has been shown to activate catalase, protein phosphatase, and caspase-3, whereas malathion induces caspase 3 only. At the same time, the studied organophosphate pesticides caused a significant decrease in glutathione transferase and succinate dehydrogenase compared to the control, especially under the action of malathion. Moreover, under the influence of malathion there was a decrease in the activity of protein phosphatase and catalase. As for glutathione reductase, the subtoxic concentration of roundup caused its activation, and the ecologically real concentration of malathion caused its inhibition. In sum, malathion caused more noticeable toxic effects in zebrafish than roundup.
 Therefore, organophosphate pesticides carry significant risks of toxic effects on fish as non-target organisms, which is important to consider when choosing agronomic maintenance options for the harvest and the potential danger to the environment.

Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats.

Malathion is an organophosphate pesticide widely used for agricultural crops and for vector control of Aedes aegypti. Humans are exposed to this environmental contaminant by ingesting contaminated food. The juvenile and peripubertal periods are critical for the postnatal development of the epididymis and are when animals are most vulnerable to toxic agents. Since juveniles and adolescents are developing under exposure to the insecticide malathion, the aim of the present study was to evaluate the effects of exposure to low doses of malathion on postnatal epididymal development in rats. Male Wistar rats were exposed to malathion daily via gavage at doses of 10 mg kg-1 (M10 group) or 50 mg kg-1 (M50 group) for 40 days (postnatal days (PNDs) 25-65). The control group received the vehicle (0.9% saline) under the same conditions. On PND 40, the epididymides were removed, weighed and used for histological analysis and determination of the inflammatory profile and sperm count. Sperm from the vas deferens were subjected to sperm motility analysis. The M50 group showed tissue remodelling in the caput and cauda epididymides and increased neutrophil and macrophage migration in the caput epididymis. The M10 group showed decreased motile spermatozoa and IL-6 levels in the caput epididymis. Both doses decreased the IL-1β level and altered the morphology of the same region. These results show that malathion exposure may impair postnatal epididymal development. Furthermore, alterations of the immune system in the epididymal environment are presented as new findings regarding the action of malathion on the epididymis.

Effects of GST Polymorphism on Ameliorative Effect of Curcumin and Carvacrol against DNA Damage Induced by Combined Treatment of Malathion and Parathion

Background: Organophosphorus pesticides has been widely used in agriculture fields to control various crop insects and their extensive use pose human life at threat because of their adverse effects on human health. In this study, we checked the effects of GST polymorphism on ameliorative effect of curcumin and carvacrol against DNA damages. Methods: Comet assay was used to assess the DNA damage and results were expressed as Tail moment. Heparinised fresh blood from healthy individuals was treated with combined concentration of malathion and parathion (i.e. 30 µg/ml of malathion and 2.5 µg/ml of parathion) in presence of combination of curcumin and carvacrol (25 µg/ml curcumin + 2.5 µg/ml carvacrol and 50 µg/ml curcumin + 5.0 µg/ml carvacrol) in order to observe the ameliorative role of curcumin and carvacrol. Multiplex PCR was performed for GSTM1 and GSTT1 genotyping. Results: Curcumin in combination with carvacrol (i.e. 25 µg/ml curcumin + 2.5 µg/ml carvacrol and 50 µg/ml curcumin + 5.0 µg/ml carvacrol) significantly reduced the DNA damage caused by combined action of malathion and parathion which supports their antigenotoxic property. No significant relationship of GSTT1 and GSTM1 polymorphism with genotoxicity of both the pesticides and antigenotoxic potential of curcumin and carvacrol was observed. Conclusion: Malathion and parathion were genotoxic in human PBL. Curcumin and carvacrol had an antigenotoxic effect against the malathion and parathion while there was not any significant effect of GSTT1 and GSTM1 polymorphism on genotoxicity of these pesticides and antigenotoxicity of curcumin and carvacrol.

Induction of insulin resistance by malathion: Evidence for disrupted islets cells metabolism and mitochondrial dysfunction

Hyperglycemia is observed with exposure to organophosphorus (OP) pesticides. The aim of this study was to investigate the effects of malathion on secretion of insulin from rat pancreatic islets in vitro and in vivo. Malathion was administered through food for 4 weeks at concentrations of 100, 200, and 400 ppm. For in vivo experiment, at the end of treatment, blood sample was obtained and plasma was separated. For in vitro experiment, the treated rats were anesthetized and underwent a laparatomy. The common bile duct was cannulated and the pancreas distended by injecting of cold collagenase V using peristaltic infusion pump. Islets were then hand picked under a stereomicroscope and cultured in the presence of various doses of glucose and KCl. Malathion at doses of 200 and 400 ppm increased plasma glucose and insulin concentrations and lowered activity of erythrocyte acetylcholinesterase. The isolated islets from pretreated animals with malathion 200 and 400 ppm showed lower glucose-stimulated insulin secretion while no change was observed in the presence of KCl. Light microscopic examination revealed that malathion causes patchy degenerative changes in pancreatic islets. Combination of in vivo and in vitro findings suggests that malathion induces a kind of insulin resistance that cannot overwhelm hyperglycemia. This action of malathion is mediated through disruption of islets mitochondrial function.

Contribution of inflammatory mast cell mediators to alterations in macrophage function after malathion administration

Recent studies using mast cell-deficient mice showed that the presence of mast cells was necessary for the increase in macrophage function observed after oral administration of malathion and reconstitution with bone marrow-derived mast cells restored the ability of malathion to increase macrophage function. In addition, the release of mast cell mediators (blocked by cromolyn) and histamine (action blocked by pyrilamine) was shown to be involved in the action of malathion on macrophage function. In the present study, the contribution of inflammatory mediators (i.e. arachidonic acid metabolites and tumor necrosis factor [TNF]) which may be generated by mast cells after oral administration of malathion, was examined. Controls in this study included the effect of the agent to be examined on: (1) resident peritoneal macrophages; and (2) macrophages elicited with pristane, an agent shown previously to stimulate macrophage function in the absence of mast cells. Intraperitoneal administration of indomethacin, an inhibitor of cycloxygenase, or neutralizing antibody to TNF 30 h before and 4 h after oral malathion blocked the ability of malathion to increase macrophage function, as measured by the generation of respiratory burst activity and the production of cathepsin D. On the other hand, administration of these agents to mice injected intraperitoneally with pristane did not affect the observed increase in cathepsin D production. Respiratory burst function after elicitation with pristane was slightly decreased (indomethacin) or not affected (antibody to TNF). The effect of intraperitoneal administration of nordihydroguaiaretic acid (NDGA), an inhibitor of both cycloxygenase and lipoxygenase, was also examined. Intraperitoneal administration of NDGA partially blocked the effects of oral administration of malathion on peritoneal macrophage function, but did not affect the function of resident or pristane-elicited peritoneal macrophages. These data suggest that inflammatory mediators (potentially released from mast cells upon stimulation) contribute to the elevation in macrophage function observed after oral malathion administration.

Effect of malathion on some kinesiological parameters of the gastrocnemius muscle of frog, rana hexadactyla (lesson) and the antagonistic action of atropine

Selected kinesiological parameters were studied in the gastrocnemius muscle of frog, R. hexadactyla Lesson, under different treatments with malathion and atropine. There is an apparent decrease of twitch amplitude, half contraction time, half relaxation time, twitch duration and fatigue time in control muscle during malathion treatment. In the denervated muscle, twitch amplitude, latency period, and fatigue time were lowered, with elevated phases of contraction and relaxation time. However, all these parameters except fatigue time showed an elevatory trend when atropine was given along with malathion revealing its antagonistic action against malathion toxicity. All these results indicate that the action of malathion is similar to that of a pharmacologically active myo- and neurotoxic agent.

Toxicity of dichlorvos vapour to insects in aerated and non-aerated wheat and comparison of the vapour action of dichlorvos and malathion

Bioassay tests demonstrated the potent vapour toxicity of dichlorvos to Sitophilus oryzae and Rhyzopertha dominica. On freshly treated wheat at 20°C the lethal effect of dichlorvos was predominantly due to a mobile form of dichlorvos. This contrasts with the action of malathion. Dichlorvos vapour at toxic concentrations was moved through grain for up to 4 m using air flows comparable to those used in aeration of bulk stored wheat. There was a marked increase in efficiency of dichlorvos under forced air-flow conditions. Effect of air flow on movement of dichlorvos through wheat is discussed quantitatively.

Laboratory and field trials to determine the modes of action of malathion and diesel oil against Culicoides furens (Poey) (Dipt., Ceratopogonidae)

One field and four laboratory trials were run to determine the reason for the success of 1–8% malathion in diesel oil in controlling Culicoides furens (Poey) in swamp mud in Jamaica. In the laboratory malathion and marine diesel oil (1:4000) was an effective larvicide for up to two days after application to the mud, but diesel oil alone gave only slight mortality even after six days. Judged by the emergence of adults, diesel oil gave 100% pupal mortality for five days and still showed some effect after four weeks; this effect was not improved by the addition of malathion. In the field, counts of emerging adults showed that diesel oil at 106–5 gal/acre gave 97–8% control of C. furens for up to eight weeks after application. It is concluded that, because of the possibility of pupicidal action, insecticides should be assessed on adult emergence rather than larval mortality.

Growth and respiration of monolayer cell cultures of chick embryo heart and skeletal muscle: action of malathion and malaoxon.

SummaryThe respiration of chick embryo heart and pectoral muscle cell cultures was determined using a polarographic respiration chamber designed specifically to measure the oxygen consumption of monolayers of cells. Heart cultures had a mean respiration rate of 215 ± 84 mμmoles O2/hr/106 cells; pectoral muscle cultures had a mean respiration rate of 163 ± 46 mμmoles Oa/hr/106 cells. Malathion 3 × 10−4M reduced the respiration of pectoral muscle cultures to 26% of normal and the respiration of heart cultures to 50% of normal. Malaoxon 3 × 10−4M had no effect upon the respiration of either cell type. Growth studies indicated that malathion was more toxic than malaoxon to the growth of both cell types.

Variation in toxicity of malathion when applied to certain body regions of Schistocerca grgaria (Forsk).

Further evidence is provided to show that the head of the desert locust is the most vulnerable body region to the toxic action of malathion (and malaoxon). The circulatory system is shown to play an important part in determining the toxicity of the insecticide by taking it towards or away from the sensitive areas. The rates of absorption, distribution and excretion of 3H-malathion for different applications are given.

Toxicology and genetics of two types of insecticide resistance in Chrysomyia Putoria (Wied.)

Resistant and normal colonies of the blowfly Chrysomyia putoria (Wied.) were obtained from the Congo and cultured in London. By selection and inbreeding, sub-colonies homogeneous for resistance to (a) the BHC/dieldrin group of chlorinated insecticides and (b) malathion, were obtained. Homogeneous resistant colonies were markedly less fertile and were difficult to perpetuate.Toxicological investigations of the BHC/dieldrin-resistant colony revealed a characteristic resistance spectrum similar to that of six other insect species. Genetical experiments with the BHC/dieldrin-resistant strain indicated inheritance by a single gene pair, intermediate in dominance, very similar to that reported in nine other insect species.Toxicological investigations of the malathion-resistant colony, including tests with 19 organophosphorus insecticides, showed a resistance largely specific to malathion and malaoxon. This was rather similar to malathion-resistant strains of two other species, for which data were available. It is known that the toxic action of malathion on mammals can be potentiated by prior treatment with small doses of certain organophosphorus compounds, which inhibit an aliesterase that detoxifies malathion by carboxylic hydrolysis, and furthermore that potentiation has been found in malathion-resistant strains of the house-fly, Musca domestica L. Accordingly, the phenomenon was investigated in C. putoria by adding non-lethal amounts of EPN or tri-o-cresyl phosphate (TOCP) to malathion. It was found that specific malathion-resistance was virtually abolished by this means, a result that suggests that such resistance in this species depends on a carboxyesterase. Genetical experiments with the malathion-resistant strain indicated inheritance by a single gene pair, with nearly complete dominance, similar to that in three other insect species.It is concluded that both types of resistance in C. putoria have much in common with those occurring in analogous strains of other insect species.

Effects of piperonyl butoxide on insecticidal potency

The effects of piperonyl butoxide on the toxicity to adult mosquitos (Anopheles stephensi List, and Aedes aegypti (L.)) and house-flies (Musca domestica L.) of pyrethrins, two synthetic pyrethroids, three organophosphorus compounds and their oxygen analogues, and three carbamates (Sevin, and both 3-isopropylphenyl and 3,5-di-isopropylphenyl N-methylcarbamate) were determined quantitatively. Solutions, in di-isobutyl ketone, containing insecticide alone and insecticide/ piperonyl butoxide mixtures in the proportion of 1:10 were applied topically to individual insects.A high degree of synergism was obtained only with natural pyrethrins and carbamates and house-flies, and the effect on the toxicity of these insecticides to adult mosquitos was much less pronounced. Insecticidal potency of the synthetic pyrethroids allethrin and dimethrin was influenced less than that of natural pyrethrins.Piperonyl butoxide antagonised the action of malathion in M. domestica, and to a slight extent in A. stephensi, but increased the toxicity of malathion and malaoxon to Ae. aegypti. It had little effect on the potency of diazinon and parathion and their oxygen analogues (diazinoxon and paraoxon) with the exception that it increased the toxicity of diazinon and diazinoxon to Ae. aegypti.The results are discussed in relation to the hypothesis that piperonyl butoxide inhibits detoxification in insects. From a practical point of view, they suggest that no great advantages would be derived from the addition of piperonyl butoxide to formulations of organophosphorus compounds and carbamates used for residual contact action against adult mosquitos.

Possible effects of the application of malathion on the small moth borer, Diatraea saccharalis (F.), and its parasite Lixophaga diatraeae (Tns.) in Jamaica

Investigations were undertaken in Jamaica to determine whether or not the application of malathion for the control of the West Indian cane-fly, Saccharo-sydne saccharivora (Westw.), disturbed the natural control exerted by Lixophaga diatraeae (Tns.) on its host Diatraea saccharalis (F.). It was demonstrated, by exposing caged adults of Lixophaga, that adults present in the field at the time of insecticidal application would be killed but because of the short residual action of malathion the reduction in the parasite population would only be temporary. A comparison of the degree of parasitism in sprayed and unsprayed fields did not reveal any evidence of reduction in the rate of parasitism. The extent of damage by Diatraea in sprayed fields was significantly less than in unsprayed ones and factors which might account for this are discussed.

Interactions of Topically Applied Piperonyl Butoxide and Malathion in Producing Lethal Action in House Flies1

The nature of the antagonistic effects of piperonyl butoxide to the toxic action of malathion on house flies, Musca domestica L., was investigated. Malathion was topically applied to various sites on the house fly body with varying degrees of toxicity. The same phenomena occurred for the relative toxicity of malathion in combination with piperonyl butoxide. The antagonistic effects of piperonyl butoxide were demonstrated even when it was applied on a separate area of the integument, separated from malathion treatment site, in nearly simultaneous topical applications to individual flies. The antagonistic effects of piperonyl butoxide decreased slowly over a 30-hourperiod, when it was applied first, followed at varying time intervals by the toxicant. In the reverse order of treatment such antagonistic effects decreased rapidly over a range of 30 minutes between the applications of malathion and piperonyl butoxide. The application of piperonyl butoxide 1 to 2 hours before malathion markedly increases the magnitude of the antagonism observed for simultaneous applications.

The Effects of Different Temperatures and Piperonyl Butoxide on the Action of Malathion on Susceptible and DDT-Resistant Strains of House Flies1

Journal Article The Effects of Different Temperatures and Piperonyl Butoxide on the Action of Malathion on Susceptible and DDT-Resistant Strains of House Flies Get access Lallan Rai, Lallan Rai Search for other works by this author on: Oxford Academic PubMed Google Scholar Saad E. D. Afifi, Saad E. D. Afifi Search for other works by this author on: Oxford Academic PubMed Google Scholar H. C. Fryer, H. C. Fryer Search for other works by this author on: Oxford Academic PubMed Google Scholar C. C. Roan C. C. Roan Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Economic Entomology, Volume 49, Issue 3, 1 June 1956, Pages 307–310, https://doi.org/10.1093/jee/49.3.307 Published: 01 June 1956

Effect of Diluents on the Acaricidal Action of Malathion and Aramite in Dusts1

Effect of Diluents on the Acaricidal Action of Malathion and Aramite in Dusts1