This article explores issues related to the knowledge-power relationships that sustain the dynamics of production of markers of social differentiation. By exploring literature that deals with the devices of knowledge-power and differentiation, the article explores situations that focus on the markers gender and sexuality, problematizing their crossings in Physical Education. The proposed analysis signals effects of the dynamics of production of differences in the modes of experiencing of bodies and movements in competence practices of Physical Education, as well as eroticism as a tool to approach this dynamic. Thus, the study aims to stimulate reflections focused on the problematization of interventional actions that propose to question the markers of difference in Physical Education, arguing for attention to not reinforcing the pattern of power they question and for the articulation with specificities of the practices in which it intervenes.