The application of long-span steel bridge in urban rail transit is becoming more and more extensive, but the structure-borne noise of steel bridge induced by passing train is also prominent. Research on the structure-borne noise problem of long-span steel bridge in urban rail transit has positive significance in promoting the sustainable development of steel bridge, and improving the quality of sound environment along rail transit. In this work, dynamic receptance principle, finite element method and statistical energy method are combined to establish the structure-borne noise prediction model of the long-span steel bridge, and the rationality and validity of the model are verified based on field measurement results. Based on the prediction model, the noise radiation characteristics of large-span steel truss bridge in urban rail transit are studied, and the following conclusions are drawn: The bridge deck has the strongest acoustic contribution ability, with the contribution rate of 33–44%, followed by the longitudinal web and transverse web, with the contribution rate of 23–30%, followed by the truss chord, with the contribution rate of 5–8%, and the truss web, longitudinal wing and transverse wing have weak contribution ability, with the contribution rate of less than 2%. The acoustic radiation efficiency of steel truss bridge components reaches its peak at the critical frequency, and the critical frequency is determined by the thickness of steel bridge plate components when the material parameters are fixed. Under ordinary track structures, the middle-to-high frequency noise distribution characteristics of large-span steel truss bridge structure are obvious. Damping pad floating slab track can effectively suppress the high-frequency noise of steel bridge, and the overall sound level can be reduced by about 11–14[Formula: see text]dB.