Acids In Total Lipids Research Articles

Комплексный методический подход в определении липидов моллюсков

Marine bivalves are rich in polyunsaturated fatty acids. Cerastoderma glaucum is a potentially commercial sea cockle that inhabits the Mediterranean and the Black Seas. This bivalve mollusk contains omega-3, omega-6, and omega-9 fatty acids. However, its lipid composition remains understudied. When determining total lipids, their classes, and fatty acid composition, standard methods often have to be adapted to the object in hand and tools available. The research objective was to develop a complex lipid analysis method for aquatic organisms.
 The study featured total soft tissues of C. glaucum harvested from the pseudolittoral zone on the sand and silt soil of the Sevastopol coast of the Black Sea. The chromatographic methods made it possible to identify total lipids and classify them into phospholipids, monoglycerides, diglycerides, sterols, and triacylglycerols. The subsequent densimetric determination involved a flatbed scanner and the TLC Manager 4.0.2.3D software. The fatty acid composition for total lipids was studied using the chromatography-mass spectrometric method.
 The existing methods in lipidology were adapted for determining total lipids, their classes, and the fatty acid composition of total lipids in C. glaucum. The article introduces a detailed description of the method for determining total lipids, as well as of how to prepare equipment and reagents to classify common lipids using multidimensional thin layer chromatography. It also contains an authentic scheme of chromatographic baths for stepwise separation, densitometric measurements, and examples of fatty acid chromatograms and mass spectra. The new sample preparation method for determining fatty acids in total lipids by gas chromatography demonstrated a minimal loss in native structure and proved to be less aggressive than standard methods of sample derivatization.
 The new method for lipid analysis of clam tissues appeared to be economical, less time-consuming, and reagent-intensive. It can be recommended for small laboratories engaged in bioenergetics or comparative analyzes of aquatic organisms.

Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture.

The carotenogenic thraustochytrid Thraustochytrium sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin, astaxanthin and β-carotene. The effects of biotin, ascorbic acid, light and temperature were evaluated in some of the experiments. In 2-day-old batch cultures, the combined mass percentage of eicosapentaenoic acid and docosahexaenoic acid in total lipids was between 16.5% (glycerol-based medium in the dark; biomass concentration = 4.2 ± 1.1 g L−1) and 42.6% (glucose-based medium under light; biomass concentration = 3.3 ± 0.1 g L−1), decreasing to 3.8% and 6.1%, respectively, after day 4. In repeated-batch cultures, the total lipids in the biomass increased after glucose or glycerol was fed alone, whereas the total carotenoids (168 ± 7 μg g−1 dry weight (DW)) and phospholipids in the biomass increased after feeding with yeast extract. The biomass with the highest content of phospholipids (28.7 ± 4.3 mg g−1 DW) was obtained using a feed medium formulated with glycerol, yeast extract and ascorbic acid. Glycerol was the best carbon source for the production of a biomass enriched with total lipids (467 ± 45 mg g−1 DW). The composition of carotenoids depended strongly on the composition of the feed. Repeated-batch cultures fed with yeast extract contained canthaxanthin as the main carotenoid, whereas in the cultures fed only with glucose, the biomass contained mainly β-carotene.

Comprehensive Analysis of Lipid Composition in Human Foremilk and Hindmilk.

Precision nutrition, also referred to as personalized nutrition, focuses on the individual to determine the individual's most effective eating plan to prevent or treat disease. A precision nutrition for infants requires the determination of the profile of human milk. We compared the lipid profiles of the foremilk (i.e., the initial milk of a breastfeed) and hindmilk (the last milk) of six Japanese subjects and evaluated whether a human milk lipid profile is useful for precision nutrition even though the fat concentration fluctuates during lactation. We detected and quantified 527 species with a lipidome analysis by liquid chromatography-tandem mass spectrometry. The fat concentration in hindmilk (120.6 ± 66.7 μmol/mL) was significantly higher than that in foremilk (68.6 ± 33.3 μmol/mL). While the total carbon number of fatty acids in triglyceride (TG) was highest in C52 for all subjects, the second or third number differed among the subjects. Both the distribution of total carbon number of fatty acids included in TG and the distribution of fatty acids in TG classified by the number of double bonds were almost the same in the foremilk and hindmilk in each subject. The lipids levels containing docosahexaenoic acid and arachidonic acid in total lipids of the foremilk and the hindmilk were almost the same in each subject. Among the sphingolipids and glycerophospholipids, the level of sphingomyelin was the highest in four subjects' milk, and phosphatidylcholine was the highest in the other two subjects' milk. The order of their major species was the same in each foremilk and hindmilk. A clustering heatmap revealed the differences between foremilk and hindmilk in the same subject were smaller than the differences among individuals. Our analyses indicate that a human-milk lipid profile reflects individual characteristics and is a worthwhile focus for precision nutrition.

Synergistic integration of wastewaters from second generation ethanol plant for algal biofuel production: an industrially relevant option.

The present study provides an integrated method for utilizing the wastewaters from second generation (2G) ethanol pretreatment plant for microalgal biomass and lipid production. The study was conducted using a mixture of wastewaters (referred as MW; pH 4.3) generated after washing of acidic and alkaline-soaked lignocellulosic biomass prior to pretreatment process. The growth studies indicated that the thermotolerant strain of Chlorella pyrenoidosa (C. pyrenoidosa) M18 exhibited higher cell proliferation in wastewater as compared to freshwater. About 20-25% enhancement in biomass (509mg L-1 d-1 ± 3.09) and lipid productivity (146mg L-1 d-1 ± 1.34) was observed in MW. The total chlorophyll content and variable fluorescence by maximum fluorescence (Fv/Fm) ratio of strain cultivated in MW were 10.32µgmL-1 and 0.75, respectively. The use of MW also enhanced the content of saturated and monounsaturated fatty acids in total lipid. The exhausted wastewater medium obtained after harvesting the auto-flocculated biomass was also reused up to three successive growth cycles. The recycled medium without any nutrient addition could be used for two subsequent rounds with enhanced biomass (520mg L-1 d-1 ± 4.07) and lipid (157.71mg L-1 d-1 ± 1.09) productivities. This synergistic approach of cultivating thermotolerant microalgae with wastewater from 2G pretreatment plant provides an economical setup for development of commercial algal biofuel technology.

Эколого-физиологическая характеристика сосудистых растений арктических тундр Западного Шпицбергена

The results of long-term studies of the ecological and physiological features of vascular plants in the Arctic tundra on the island of Spitsbergen are presented. Data on the content of plastid pigments for 98 vascular plants species from 20 families. The range of chlorophyll content is 0,4–2,6 mg / g of fresh weight. The relationship between the content of pigments and some indicators of functional activity of Arctic plants is analyzed. The content of flavonoids in the leaves of 63 species of vascular plants was studied. It is shown that 85 % of the studied species have high and medium values (4–11 %) of the flavonoid content. This is 2–3 times higher than in similar species of the Kola Subarctic. The composition of lipids in the leaves of the dominant and rare plant species of the Arctic tundra during the active vegetation period is analyzed. It was found that the dominance of saturated or unsaturated fatty acids in total lipids depends on the cold resistance of plant species and their representation in the ecotopes of the studied territory.

Extracted microbial oil from a novel Schizochytrium sp. (T18) as a sustainable high DHA source for Atlantic salmon feed: Impacts on growth and tissue lipids

The use of microbial oil, high in docosahexaenoic acid (DHA), was investigated as a lipid source in diets for farmed Atlantic salmon. Schizochytrium sp. oil is produced from heterotrophic fermentation and it can be a renewable source of long-chain n-3 fatty acids due to its high DHA content. A 16-week feeding trial was conducted on Atlantic salmon (24 g initial weight) in freshwater to test the effects of microbial oil (MO) from Schizochytrium sp. (T18), on fish growth performance and tissue lipid and fatty acid composition. Four experimental diets were tested: a reference diet (20% FO) and a commercial-type control diet (10% FO + 10% vegetable oil), a low MO diet (5% MO) and a high MO diet (10% MO). There were no differences in weight gain (81–98 g), condition factor (1.08), specific growth rate (1.30–1.53%/day), nor feed conversion ratio (0.84–0.90) among all treatments (p > 0.05). Fatty acids in total lipid in the muscle reflected the fatty acid profile in the diet, particularly in salmon fed low and high MO diets, with high muscle storage of DHA and low EPA compared to salmon fed the FO and FO/VO control diets. Overall, Schizochytrium sp. (T18) is a candidate for use in dietary oil blends for juvenile salmon.

Incorporation and distribution of crude palm oil fatty acids in the plasmatic lipids of consumers

Incorporation and distribution of crude palm oil fatty acids in the plasmatic lipids of consumers - IJCBR- Print ISSN No: - 2394-6369 Online ISSN No:- 2394-6377 Article DOI No:- 10.18231/j.ijcbr.2019.063, International Journal of Clinical Biochemistry and Research-Int J Clin Biochem Res

Comparison of lipid compositions of soft coral <i>Sinularia brassica</i> collected in different coastal regions of Vietnam

Five soft coral samples belonging to the same specimen Sinularia brassica were collected in the coastal area of Ha Long Quang Ninh, Nhat Le beach, Quang Binh, Hon Vang - Thanh Hoa and Nha Trang areas (2014–2016), these samples were examined and analyzed by total lipid content, content and composition of lipid and fatty acids. Highest total lipid content was found in the sample SHM 09 collected in Nha Trang bay (1.11%), and the lowest total lipid content was in HL-SHM 07 collected in Ha Long, Quang Ninh (0.68%).The lipid composition of samples contained basic classes such as polar lipids, sterols, free fatty acids, triacylglycerols, monoankyldiacylglycerols, hydrocarbons and waxes. In studied soft corals, there are the appearance of all fatty acids from C12 to C24. There are differences in composition and content of lipids and fatty acids in samples collected in different locations: Samples collected at Nha Trang bay contain more polyunsaturated fatty acids than the other samples collected in Ha Long, Thanh Hoa, Quang Binh. Another two samples, collected in Hon Vang (Thanh Hoa) have similar composition and content of lipid classes, the composition and content of fatty acids in total lipids. The obtained results also show the significant impact of habitat and time of acquisition on the studied subject.

Moderate chronic ethanol consumption exerts beneficial effects on nonalcoholic fatty liver in mice fed a high-fat diet: possible role of higher formation of triglycerides enriched in monounsaturated fatty acids.

Several clinical studies suggested that light-to-moderate alcohol intake could alleviate nonalcoholic fatty liver disease (NAFLD), but the underlying mechanism is still poorly understood. Mice fed a high-fat diet (HFD) were submitted or not to moderate ethanol intake for 3months (ca. 10g/kg/day) via drinking water. Biochemical, analytical and transcriptomic analyses were performed in serum and liver. Serum ethanol concentrations in ethanol-treated HFD mice comprised between 0.5 and 0.7g/l throughout the experiment. NAFLD improvement was observed in ethanol-treated HFD mice as assessed by reduced serum transaminase activity. This was associated with less microvesicular and more macrovacuolar steatosis, the absence of apoptotic hepatocytes and a trend towards less fibrosis. Liver lipid analysis showed increased amounts of fatty acids incorporated in triglycerides and phospholipids, reduced proportion of palmitic acid in total lipids and higher desaturation index, thus suggesting enhanced stearoyl-coenzyme A desaturase activity. mRNA expression of several glycolytic and lipogenic enzymes was upregulated. Genome-wide expression profiling and gene set enrichment analysis revealed an overall downregulation of the expression of genes involved in collagen fibril organization and leukocyte chemotaxis and an overall upregulation of the expression of genes involved in oxidative phosphorylation and mitochondrial respiratory chain complex assembly. In addition, mRNA expression of several proteasome subunits was upregulated in ethanol-treated HFD mice. Moderate chronic ethanol consumption may alleviate NAFLD by several mechanisms including the generation of non-toxic lipid species, reduced expression of profibrotic and proinflammatory genes, restoration of mitochondrial function and possible stimulation of proteasome activity.

Content and Composition of Fatty Acids in Total Lipids from the Buds of Betula pubescens, B. pendula, and B. pendula var. carelica

Absolute content and composition of fatty acids (FAs) in total lipids from the buds of white birch (Betula pubescens Ehrh.), silver birch (B. pendula Roth), and its subspecies B. pendula var. carelica Merckl. were investigated. The examined birches differed in these characteristics calculated per gram of dry weight. The buds of Karelian birch contained reliably greater content of FAs in total lipids as compared with the buds of two other birches. Detected differences in the content of total lipid FAs in two species and one variety of birch reflect peculiarities of biosynthesis of lipid compounds and may serve as an indicator for identification of plants from the genus Betula L.

Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer

Nervonic acid (C24:1, Δ15), a monounsaturated long chain fatty acid, is a major component of mammalian neural tissue. Lack of nervonic acid in human usually leads to neurological disorder. Recently, production of nervonic acid from plant for pharmaceutical, nutraceutical, and functional food received increasing attention. We have previously isolated a microalgal strain Mychonastes afer HSO-3-1, which can accumulate approximately 6% of nervonic acid and <0.5% of erucic acid in triacylglycerol. In this study, the full length of 3-ketoacyl-coA synthase gene MaKCS, encoding the first component of fatty acid elongation complex, was cloned and expressed in Saccharomyces cerevisiae BY4741 under the control of GAL1 promoter. S. cerevisiae harboring pYC230-Gal-MaKCS resulted in the production of 1.5% nervonic acid in total lipid. The expression of KCS gene in M. afer was found to be induced under stress conditions such as nitrogen deficiency and high light, which was revealed by real-time PCR analysis. MaKCS was demonstrated to be able to extend very long chain monounsaturated fatty acids including nervonic acid, which provides insights into how nervonic acid is synthesized in microalgae.

The content of fat acids of general lipids and heavy metals in pollen from dande-lion under different technogenic impact on environment

The level of environmental impact was determined by the content of heavy metals (iron, zinc, copper, chromium, nickel, lead, arsenic and cadmium) in the pollen from dandelion (TaraxacumofficinaleWigg.). Samples of pollen from dandelion for laboratory tests were taken in apiaries located in areas with different intensity of traffic and industry. In particular, the training apiary of Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytsky (the area with very heavy traffic and operation of industrial enterprises) and private beekeeping farms in Vynnyky town and Chyzhykiv village in Pustomiti district Lviv region (areas with less intensity of traffic and industry). Private beekeeping farms in Vynnyky town and Chyzhykiv village are located at a distance of 2–3 and 5–6 km from the training apiary of Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytsky. Moreover, both the training apiary of Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytsky and private beekeeping farms in Vynnyky and Chyzhykiv of Pustomiti district Lviv Oblast are located along the highway Lviv–Ternopil. In each of the mentioned above areas samples of the pollen of dandelion. And in each apiary the samples of the pollen of dandelion and newly built honeycombs were taken from three hives. To clarify the origin of species of the dandelion pollen the identification study was performed using the computer programs «LUCIA» (Laboratory Colour Image Analysis) and «Pollen Data Bank». In the selected samples of the bee pollen heavy metals were found and measured. In the selected samples of newly built honeycombs the concentration of heavy metals, anionic and nonetherified fatty acids and fatty acids of total lipids was measured. The content of heavy metals in the studied biological material was measured on atomic absorption spectrophotometer C–115 PC. The concentration of anionic and nonetherified fatty acids and fatty acids of total lipids in the studied biological material were measured by the gas–liquid chromatography. The resulting digital material was studied and analyzed by variation statistics using the Student's criterion. The arithmetic mean value and arithmetic errors were calculated. Changes were considered probable at p &lt;0.05. For calculations a special computer program Origin 6.0, Excel (Microsoft, USA) was used. The pollen of the dandelion which grows in the area with medium and low technogenic load in comparison with the pollen of the dandelion which grows in the area with high technogenic load the amount of iron, zinc, copper, chromium, nickel, lead and cadmium decreases. The pollen of the dandelion which grows in the area with medium and low technogenic load in comparison with the pollen of the dandelion which grows in the area with high technogenic load the amount of fatty acids of common lipids increases mainly due to monounsaturated fatty acids of n–7 and n–9 families and polyunsaturated fatty acids of n–3 and n–6 families. Therefore their energetic, attractive, functional–metabolic and biologic value for the bee organism increases. The most considerable change in the amount of heavy metals and fatty acids of common lipids is observed in the pollen of the dandelion which grows in the area of low technogenic load.

Fatty acid composition of total lipids in embryogenic and nonembryogenic callus lines of larch

Fatty acid (FA) composition of total lipids in embryogenic and nonembryogenic calli of Siberian larch (Larix sibirica Ledeb.) was investigated by the method of GC-MS. We detected a high content of oleic acid in total lipids of embryogenic cell culture (32–56% by weight of total FA), which apparently depends on a high activity of acyl carrier protein (stearoyl-ACP-Δ9-desat-urase). At the same time, activity of Δ12-desaturase in the cells of embryogenic calli was considerably (2–3 times) lower than in nonembryogenic calli. We discuss a possibility that concentration of FA (oleic and linoleic acids) may be used as a marker of embryogenic potential when promising cell lines of Siberian larch are screened in the stage of early embryogenesis.

Lipid composition in muscle and liver of sympatric coregonid fishes from Lake Baikal (Coregonus spp.) under common garden experiment

The content of total lipids, total phospholipids, and fatty acids of total lipids in muscles and liver of juvenile Lake Baikal sympatric coregonid fishes have been analyzed for the first time under a common garden experiment. Baikal omul, Coregonus migratorius Georgi, is an active migrant of the pelagic zones of the lake. Baikal (lacustrine) whitefish, C. baicalensis Dybowski, is a colonizer of the bottom habitats, which are relevant to the pelagic zones of the littoral and underwater slope. Structural lipids (phospholipids and cholesterol) dominated in total lipids of the tissues of all fish under study. Spare lipids significantly prevailed in omul muscles when compared to whitefish. The highest variability of fatty acid composition was reported in muscles of coregonid fishes. Statistically significant differences were revealed in the content of the ω3 polyunsaturated fatty acids in muscles of lacustrine whitefish and omul. Associations of lipid compositions revealed in tissues of the whitefishes under the study with their respective ecotypes have been discussed.

Placental MFSD2a transporter is related to decreased DHA in cord blood of women with treated gestational diabetes

Maternal-fetal transfer of docosahexaenoic acid (DHA) is impaired by gestational diabetes mellitus (GDM), but the underlying mechanisms are still unknown. MFSD2a was recently recognized as a lyso-phospholipid (lyso-PL) transporter that facilitates DHA accretion in brain. The role of this transporter in placenta is uncertain. We evaluated effects of GDM and its treatment (diet or insulin) on phospholipid species, fatty acid profile in women, cord blood and placental fatty acid carriers. Prospective observational study of pregnant women recruited in the third trimester (25 controls, 23 GDM-diet, 20 GDM-insulin). Fetal ultrasound was performed at gestational week 38. At delivery, maternal and neonatal anthropometry was performed, and fatty acids in total lipids and phospholipid species were analyzed in placenta, maternal and venous cord blood. Western-blot analyses were performed for placental fatty acid carriers. Fetal abdominal circumference z-score at 38 weeks tended to higher values in GDM (P=0.071), pointing toward higher fetal fat accretion in these babies. DHA percentage in cord serum total lipids (P=0.029) and lyso-PL (P=0.169) were reduced in GDM. Placental MFSD2a was reduced in both GDM groups and was positively correlated to DHA values in cord serum total lipids (r=0.388, P=0.003). Among established placental lipid carriers, only FATP4 was correlated to DHA concentration in placental lyso-PL. In all compartments, DHA percentage was inversely correlated to fetal abdominal circumference. In offspring of women with GDM treated either with diet or insulin, higher fetal fat accretion and lower placental MFSD2a contribute to reduce DHA availability. Lyso-PL appear to contribute to materno-fetal DHA transport.

Hempseed Products Fed to Hens Effectively Increased n-3 Polyunsaturated Fatty Acids in Total Lipids, Triacylglycerol and Phospholipid of Egg Yolk.

Hempseed products represent potential alternative feed ingredients for poultry. However, their usage is not currently approved due to a lack of data to support their safety and efficacy. In this regard, the current study was conducted to assess the impact of dietary concentration of hempseed (HS) products and duration of their feeding to hens on the polyunsaturated fatty acid (PUFA) composition of egg yolk lipids. In the current study, 48 Lohmann LSL-Classic hens were individually housed in metabolism cages, in a completely randomized design, and provided one of six diets (wheat-barley-soybean-based) containing either HS (10, 20 and 30%), hempseed oil (HO; 4.5 and 9.0%) or no hempseed product (control) over 12weeks. Increasing alpha-linolenic acid (ALA) intake via increasing dietary hempseed product inclusion, significantly (p<0.0001) increased the n-3 PUFA contents of yolk total lipid. The values of ALA increased by 12-fold (152±3.56 and 156±2.42mg/yolk) and docosahexaenoic acid (DHA) by twofold to threefold (41.3±1.57 and 43.6±1.61mg/yolk) over the control, for the highest levels of HS and HO inclusion, respectively. Increasing levels of hemp products in laying hen diets proved effective in manipulating the fatty acid profile of the total lipid, triacylglycerol (TAG) and total phospholipid (PL) fractions of yolks, enhancing the n-3 fatty acids and reducing the n-6/n-3 ratio. The latter benefit was achieved within 4weeks of feeding hens either HS- or HO-containing diets.

Жирные кислоты общих липидов видов рода Cystoseira C. Agardh (Phaeophyta) (Черное море, Крым)

Жирные кислоты общих липидов видов рода Cystoseira C. Agardh (Phaeophyta) (Черное море, Крым)

Detailed Study of Furan Fatty Acids in Total Lipids and the Cholesteryl Ester Fraction of Fish Liver

Furan fatty acids (F-acids) are valuable, bioactive minor components in lipids which are characterized by a furan moiety in the acyl chain. Here, we present a gas chromatography/mass spectrometry method operated in the selected ion monitoring mode (GC/MS-SIM) for the thorough determination of F-acid as methyl esters in fish. The method takes advantage of the molecular ion and two characteristic fragment ions and enables the unequivocal detection of >350 saturated, mono- and dienoic F-acids in four GC/MS-SIM runs. This method was applied to the analysis of the methyl esters obtained from the triacylglyceride (TG) and the cholesteryl ester (CE) fraction of ten individual fish liver samples. Up to 23 F-acids, including several new or scarcely detected homologues, were detected in the samples. The concentrations of total F-acids ranged from 25 to 280 mg/100 g lipids in the TG fractions and <0.11 to 115 mg/100 g lipids in the CE fractions.

Microalgae lipid characterization.

To meet the growing interest of utilizing microalgae biomass in the production of biofuels and nutraceutical and pharmaceutical lipids, we need suitable analytical methods and a comprehensive database for their lipid components. The objective of the present work was to demonstrate methodology and provide data on fatty acid composition, lipid class content and composition, characteristics of the unsaponifiables, and type of chlorophylls of five microalgae. Microalgae lipids were fractionated into TAG, FFA, and polar lipids using TLC, and the composition of fatty acids in total lipids and in each lipid class, hydrocarbons, and sterols were determined by GC-MS. Glyco- and phospholipids were profiled by LC/ESI-MS. Chlorophylls and their related metabolites were qualified by LC/APCI-MS. The melting and crystallization profiles of microalgae total lipids and their esters were analyzed by DSC to evaluate their potential biofuel applications. Significant differences and complexities of lipid composition among the algae tested were observed. The compositional information is valuable for strain selection, downstream biomass fractionation, and utilization.

FemaleLeuciscus lepidusMay Be a New Alternative for Fish Oil Supplements

The proximate composition of male and femaleLeuciscus lepidusin Beyşehir Lake was investigated. The fatty acid profiles of total lipid, phospholipid, and triacylglycerol in muscle and liver of male and femaleL. lepiduswere evaluated by gas chromatography. Proximate analyses showed that meat of male and femaleL. lepidushad 15.13 ± 0.04 and 18.75 ± 0.11% fat, 20.42 ± 0.45 and 22.21 ± 0.56% protein, 65.47 ± 1.37 and 61.28 ± 1.03% moisture, and 1.51 ± 0.05 and 1.50 ± 0.03% ash, respectively. The percentage of total saturated fatty acids was higher in liver than in muscle, whereas the total polyunsaturated fatty acid (PUFA) content was the lowest in all fatty acid profiles. The phospholipids contained more PUFAs than triacylglycerol. Analysis of variance indicated significant differences (P&lt;0.05) between male (47.51%) and female (49.98%) muscle PUFAs in total lipid. The proportion of omega 3 (ω3) to omega 6 (ω6) fatty acids of total lipid was 3.15 in male and 3.68 in female. The ratio is an important indicator for comparing the value of fish oil. Therefore, it was concluded thatL. lepiduswas considered to be a high quality product for healthy food choice. Additionally, femaleL. lepidusmay especially be used to produce fish oil supplements from freshwater fish combined with vegetable oils.