Acids In Cows Research Articles

Investigation of the interconnection of the metabolism of Carotine, vitamins A and E in cows at the beginning of the lactation period with the level of beta-hydroxybutyrate

This article presents the results of a screening study of the blood serum of cows for the content of carotene in the period of 28-62 days after calving. Out of 31 samples, the results of six samples corresponded to the normative intervals, which amounted to 19.35%. In the remaining animals, carotene was determined in the range from 0.06 to 0.34 mg/dL. In accordance with the obtained data on the concentration of carotene, samples were taken from the sample and 3 groups of animals were formed in such a way that, in an intergroup comparison, Student's t-test was less than 0.001. In the blood serum, a study was made of the concentration of vitamin A, vitamin E and beta-hydroxybutyric acid. Studies have shown that the lowest values of carotene correspond to the minimum concentrations of vitamins A and E. An increase in the level of carotene by 2.36 times to 0.26 ± 0.01 mg / dl is accompanied by an unreliable increase in the concentration of vitamins A and E by 33.3 and 12.8 %, respectively. The highest concentration of carotene in animals of the third group (0.45±0.02 mg/dl) contributes to an increase in the content of fat-soluble vitamins in the blood - A (two times) and E (1.65 times), compared with the indices of cows of the group 1 (both P<0.001). When considering the content of beta-hydroxybutyric acid in cows, it can be noted that with carotene values below the physiological range in groups 1 and 2, an increase in its concentration above the reference values is observed, while the highest level of carotene corresponds to the concentration of beta-hydroxybutyrate within the normative limits and below, than in groups 1 and 2 by 51.6% and 59.1% (P<0.05). The conducted studies made it possible to determine the high frequency of occurrence of a reduced level of carotene in cows during the milking period, which amounted to 80.65%. The concentration of carotene below physiological limits leads to a decrease in the concentration of important fat-soluble vitamins in the blood, and also contributes to the development of ketonemia.

Overview of SNPs Associated with Trans Fat Content in Cow’s Milk

Lipids consumed with milk derivatives are one of the main parts of the human diet. Trans fatty acids in milk are causing a debate about their impact on the incidence of cardiovascular disease, pathological abnormalities, and cancer. The fatty acid profile of milk is influenced by a large number of different factors, one of which is genetic. The development of genetic studies, including Genome-Wide Association Studies (GWAS), may help define genomic regions associated with fatty acid content in milk, including trans fatty acids. This article provides an overview of international studies on the identification of genomic regions and SNPs associated with the trans fatty acids in cow’s milk. The results are based on research of cattle such as Norwegian Red cattle, Holstein, Jersey, and Brown Swiss. The presented review shows that 68 SNPs were localized on chromosomes 1, 2, 4–6, 8–10, 12, 14–20, 22–25, and 27–29. Further research in this direction will provide new information that will serve as an impetus for the creation of modern breeding technologies and increase the performance of the manufacture of high-quality dairy products. The search for genetic markers associated with the content of TFA in milk is a promising direction in agricultural science and will allow more complete breeding work with cattle.

Study of the activity of lipolysis and gluconeognesis in new-child cows with subclinical and clinical ketosis

The article discusses the results of a study of markers of lipolysis and gluconeogenesis in new-calf cows against the background of subclinical and clinical ketosis. The indicator of beta-hydroxybutyric acid in blood serum was chosen as a marker for the formation of groups based on the presence of ketosis and its form. Cows with an indicator of up to 1.0 mmol/l were considered healthy, the presence of subclinical ketosis was determined at a level of beta-hydroxybutyrate from 1.1 to 1.5 mmol/l, clinical ketosis - over 1.6 mmol/l. Cows were bled 3-7 days after calving and a study was made of the concentration of beta-hydroxybutyric acid, glucose, fructosamine, non-esterified fatty acids, cortisol and ALT activity. The results of the study revealed a significant increase in the concentration of non-esterified fatty acids in cows with moderate ketonemia by 46.9%, and in cows with a pronounced increase in the content of beta-hydrosibutyrate in the blood - by 79.8%. When considering the concentration of glucose in cows with different levels of beta-hydroxybutyrate in the blood, there is a slight increase in the indicator by 14.5% (P> 0.05) in cows with subclinical ketosis, but in animals with severe ketonemia, a significant decrease in glucose by 38.3 %. Cows in subclinical and clinical ketosis had significantly higher levels of cortisol (1.9 and 2.4 times, respectively) than cows with low blood levels of betahydroxybutyric acid. At the same time, cows with clinical ketosis showed a significant increase in the concentration of fructosamine by 22.3%. Subclinical ketosis, manifested by a moderate increase in the concentration of beta-hydroxybutyric acid in the blood (1.1 - 1.5 mmol / l), causes adaptive reactions in the body aimed at maintaining glucose homeostasis and extracting lipids from the depot that compensate for a temporary energy deficit. Clinical ketosis is accompanied by dissociation of the regulatory mechanisms of anabolic and catabolic processes in the body of a freshly calved cow.

Contribution of Chevreul to lipid chemistry

Michel Eugène Chevreul entered at 17 the laboratory of Vauquelin, at the Museum. He began his investigations on animal fats in 1811. They appear as yet the most complete and the best executed experiments found in all chemistry. For the first time, he combined fractional solution, crystallization, distillation, melting point determination, and ultimate analysis. All started with the isolation of an acid and solid substance, “margaric acid”, the first of a long list of fatty acids isolated from various fats. Studying saponification, he determined that “anhydrous glycerine” was combining with water. After studying human gallstones, he discovered “cholesterine” (cholesterol). From whale spermaceti, he described “cetin” (mainly cetyl palmitate), containing no glycerol. He isolated from sheep fat a new acid he named “stearic acid”. This component was proposed in a joint patent by Chevreul and Gay-Lussac (1825) for the fabrication of very efficient candles. He discovered several volatile fatty acids in cow and goat butter (from C4 to C10). His master work (Recherches chimiques sur les corps gras d’origine animale, 1823b) contains his experimental procedures and his theoretical conclusions on the nature of fats, it may be considered as the first treatise on lipochemistry.

Comparison of the Effectiveness and Environmental Impact of Selected Methods for the Determination of Fatty Acids in Milk Samples

Determination of the fatty acid profile in milk samples is one of the most important in food analysis. There are many methodologies for FA determination. The conventional procedure for determining the FA composition of milk is isolation of fat or indirect methylation, trans-methylation, extraction of fatty acids, and analysis by gas chromatography. In this study, eight methods based on alkaline methylation were compared for the analysis of fatty acids in cow's milk. The response factors (RF) for GC analysis using FID were calculated. For most acids, RFs were close to 1, with the exception of short-chain fatty acids (C4:0-C8:0). To facilitate the selection of the method for the determination of fatty acids in milk samples, the methods were assessed using the environmental assessment tools of the analytical procedure: the Analytical Eco-Scale, Green Analytical Procedure Index (GAPI), and Analytical Greenness for Sample Preparation (AGREEprep). The method based on direct milk methylation received the highest scores. Omitting the lipid separation step has an impact on reducing the quantity of used toxic chemicals and reagents, and produces a smaller amount of waste, a much higher throughput, and a reduced cost analysis.

Triglyceride, fatty acid profile, antioxidant characteristics, vitamins, lipid oxidation and induction period of ice cream produced from cow and Buffalo Milk

AbstractIn this study, ice creams were produced from cow and buffalo milk using 15% sugar, 13% fat, 11% milk solids not fat, and 0.5% cremodan (TS 39.5%). Triglycerides from C26 to C36 were significantly higher in buffalo milk ice cream than cow milk ice cream. Milk type and storage both affected the fatty acid composition of ice cream samples. Concentrations of unsaturated fatty acids in cow and buffalo milk ice cream samples were 24.4% and 29.9%, respectively. Total antioxidant capacity of buffalo milk ice cream was 18% higher than cow milk ice cream. Concentrations of vitamin A and E were significantly higher in buffalo milk ice cream samples. Buffalo milk ice cream had lower peroxide value and higher oxidative stability than cow milk ice cream. Induction period of cow and buffalo milk ice cream was 8.55 h and 10.79 h with no difference in sensory properties of both types of ice creams.Practical applicationsBuffalo milk is widely used for the production of several value‐added dairy products and is the major source of milk in some Asian countries. However, due to limited research work, buffalo milk processing industry could not develop, and buffalo milk based dairy products are not available in markets of these countries. This research work can be helpful for the dairy industries to produce ice cream from buffalo milk at commercial level.

ИЗМЕНЕНИЕ БЕЛКОВОГО СОСТАВА МОЛОКА КОРОВ ПРИ СУБКЛИНИЧЕСКОМ МАСТИТЕ

The nutritional value of milk and raw milk is determined by protein, so we paid special attention to determining the protein composition of raw milk. Were made measurements: the mass fraction of total nitrogen, the mass fraction of non-protein nitrogenous compounds, the content of urea, essential and non-essential amino acids. Biochemical studies of the protein composition of milk obtained from cows with suspected mastitis indicate that the content of total protein, casein, albumin and globulins in milk significantly changed with the growth of pathological processes in the mammary gland. Lactic cows underwent an express increase in the state of health, udder using Somatest manufactured and based on the result obtained, three groups of animals of 10 goals were formed. in everyone. 1st group detainees questionable test for Somatest express method; animals 2nd – weakly positive; the 3rd group included blood with a negative reaction to the tests. The difference in protein content was 5.71% (P < 0.05) and 3.27%; casein content – 13.27% (P < 0.001) and 11.98% (P < 0.05). According to the content of serum proteins, the opposite picture was observed, with the increase in udder pathology, the amount of albumins increased by 13.12% in the first group compared to the control; and by 26.39% – in the second group. In terms of the content of globulins, an increase was also noted in comparison with the control in the first group by 25.00% (P < 0.001) and 34.34% (P < 0.001). In milk samples of three test groups of animals, we identified 15 amino acids, of which 11 were essential and 4 non-essential. The test results indicate slight changes in the content of essential amino acids in cows with an increase in the inflammatory reaction in the udder. Diseases of the udder of cows are reflected in the quantitative ratio between the constituent parts of the protein fractions of milk, in particular, this is manifested in a decrease in casein, as well as in an increase in the content of whey proteins. Thus, the amount of casein needed to make cheese is reduced and whey protein is increased, making the milk unsuitable for cheese making.

Compositional analysis of fatty acids in cow’s milk when feeding a complex additive based on activated diatomite

The article presents the research results of the effect of activated diatomite as part of a complex supplement on the composition and ratio of saturated and unsaturated fatty acids in cow's milk. The experiments were carried out in a dairy farm on black-and-white cows. The additive includes a carrieractivated diatomite; fillers are DAPS-organic selenium, sunflower oil and feed yeast and diatomite crumbs. The supplement was included in the diet of cows at the rate of 2% of the dry matter of the diet once a day. The control group did not receive the supplement. The positive effect of the use of the additive on the composition of fatty acids in cow's milk was established. There was an increase in the fraction of saturated fatty acids, which indicates an increase in volatile fatty acids: acetate and 3-hydroxybutyrate. The use of a complex supplement based on activated diatomite contributed to an increase in concentration of capric, lauric, myristic, palmitic and margarine fatty acids. The use of additives for dairy cows helps to increase their productivity, improve the quality of milk composition due to the intensive conversion of feed nutrients.

АНАЛИЗ ЖИРНОКИСЛОТНОГО СОСТАВА МОЛОКА КОРОВ НА ФОНЕ ДОБАВКИ МОДИФИЦИРОВАННОГО ДИАТОМИТА

The article presents the results of studying the effect of modified diatomite as part of a complex additive at the ratio of saturated and unsaturated fatty acids in cow’s milk. Production and physiological tests were carried out on the basis of a dairy farm LLC “Agrofirma Tetyushskoe” in the Ulyanovsk region on black-and-white cows of 2...3 lactation. For the physiological experiment, animals were selected by the method of analogues by live weight, number and month of lactation, pregnancy, and physiological state. The complex additive included modified diatomite, diacetophenonyl selenide containing organic selenium (DAFS), sunflower oil, and feed yeast. Additive based on 250 g/head/day mixed with feed once a day was administered in the diet of animals of the experimental group, the other group was a control and received no additive. The additive had an effect on the fatty acid composition of milk and contributed to an increase in the fraction of saturated fatty acids with a short chain. In the fat fraction, the amount of saturated short-chain fatty acids increases by 4.0% due to an increase in the content of capric (by 6.7%), lauric (by 12.3%, P<0.05), myristic (by 13.3%, P<0.05), palmitic (by 17.1%, P<0.05), which is a positive factor and indicates an active synthesis of volatile fatty acids, primarily acetate and 3-hydroxybutyrate. At the same time, there was a sharp decline in the content of stearic (27.3%, P<0.05) and arachinic (27.6%, P<0.05) acids. The milk of cows in the experimental group showed a decrease in the level of monounsaturated fatty acids by 7.4%.

Near infrared aquaphotomics study on common dietary fatty acids in cow's liquid, thawed milk

This study was aimed at two objectives: 1) explore the possibility of near infrared (NIR) spectroscopy to determine the content of common dietary fatty acids (FAs) and fat content in cow's liquid milk for better individual cow's feed management; 2) using aquaphotomics obtain better understanding of interaction between water molecular structure and FAs and gain more insight into their functionality.For these purposes, 252 milk samples were collected from 9 cows during 14 weeks and FAs content measured using gas chromatography as a reference method. Applying aquaphotomics NIR spectral analysis, quantification was attempted using 1300–1850 nm region where both water and FAs absorbance bands are located. The results showed possibility of quantification for 8 common dietary FAs which was more accurate when spectral region was narrowed to 1600–1800 nm. In the external validation, good predictions (R2> 0.75, RPD>1.5), were obtained for caproic and caprylic and acceptable for capric, lauric, myristic, myristoleic, palmitoleic and oleic acid (0.55<R2<0.75, RPD>1.5). Interpretation of influential variables in regression models revealed contribution of water absorbance bands related to protonated and hydration water, which originate from the water-FAs interaction and are important for their self-organization into assemblies with different morphologies.

Effect of Heat Drying Process on Fatty Acid Structure of Vegetable Oil in Infant Formula

Milk is the main nutritional base which is rich in essential nutrients for the baby. Infant formula is intended as an effective substitute for infant feeding. This study aimed to determine the amount of fatty acids in cow’s milk and to compare them with fatty acid profiles of breast milk, infant formula and vegetable oil used in production of baby’s dry milk. In this study, 3 types of cow’s milk, 3 samples of breast milk, 3 samples of infant formula and 1 and 2 samples of vegetable oil for infant formula were measured based on the presence of fatty acids in cow’s milk, fatty acid profiles of breast milk and infant formula and vegetable oil. Oil samples were homogenized and prepared by Easy MIX, France. The study found that temperature on 90 °C and 180 °C had no effect on fatty acid profiles of vegetable oils and infant formula, and was very similar to breast milk, whereas the cow’s fatty acid profiles differ in the amount of saturated and trans fatty acids compared to breast milk, it is clear that whole milk cannot replace breast milk just by diluting it with water and adding a little sugar.

A Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) Method for the Determination of Free Hydroxy Fatty Acids in Cow and Goat Milk.

A liquid chromatography–high resolution mass spectrometry (LC-HRMS) method for the direct determination of various saturated hydroxy fatty acids (HFAs) in milk was developed for the first time. The method involves mild sample preparation conditions, avoids time-consuming derivatization procedures, and permits the simultaneous determination of 19 free HFAs in a single 10-min run. This method was validated and applied in 17 cow milk and 12 goat milk samples. This work revealed the existence of various previously unrecognized hydroxylated positional isomers of palmitic acid and stearic acid in both cow and goat milk, expanding our knowledge on the lipidome of milk. The most abundant free HFAs in cow milk were proven to be 7-hydroxystearic acid (7HSA) and 10-hydroxystearic acid (10HSA) (mean content values of 175.1 ± 3.4 µg/mL and 72.4 ± 6.1 µg/mL in fresh milk, respectively). The contents of 7HSA in cow milk seem to be substantially higher than those in goat milk.

Hepatic Cystathionine β-Synthase Activity Is Increased by Greater Postruminal Supply of Met during the Periparturient Period in Dairy Cows

ABSTRACTBackgroundPostruminal supply of Met during the periparturient period enhances production efficiency (feed conversion to milk) in dairy cows partly through alleviation of oxidant and inflammatory status. Whether alterations in hepatic 1-carbon metabolism (major contributor of antioxidants) and/or energy metabolism contribute to these beneficial effects is unknown.ObjectivesTo investigate alterations in hepatic 1-carbon and energy metabolism and associations with plasma amino acids (AAs) and production efficiency in response to enhanced postruminal supply of Met.MethodsHolstein cows (n = 30 per group) were fed during the last 28 d of pregnancy a control diet (CON) or the control plus ethylcellulose rumen-protected Met (MET; 0.9 g/kg of dry matter intake). Plasma (n = 15 per group) and liver tissue (n = 10 per group) were collected throughout the periparturient period to evaluate AA profiles, activity of the tricarboxylic acid cycle, and 1-carbon metabolism via mRNA abundance, enzyme activity, and targeted metabolomics.ResultsCows in the MET group had greater overall (27%, P = 0.027) plasma Met concentrations, but had similar total plasma AA concentrations. Although mRNA abundance of 1-carbon metabolism enzymes did not differ, hepatic activity of cystathionine β-synthase (CBS) (51.2 compared with 44.4 mmol/h/mg protein; P = 0.032) and concentration (19%, P = 0.048) of the cellular antioxidant glutathione were greater overall in the MET group. mRNA abundance of aconitase 2 and fumarate hydratase was greater overall (P = 0.049), and phosphoenolpyruvate carboxykinase 1 tended (P = 0.093) to be greater overall in cows fed MET. There was a tendency (P ≤ 0.093) for greater overall hepatic concentrations of malic acid, α-ketoglutaric acid, and isocitric acid in cows fed MET.ConclusionsGreater activity of CBS in response to enhanced postruminal supply of Met likely contributes to alleviating oxidant status by increasing concentrations of glutathione. Hence, transsulfuration plays an important role in the observed improvements in production efficiency of dairy cows during the periparturient period.

Productive performance and digestive response of dairy cows fed different diets combining a total mixed ration and fresh forage

The purpose of this experiment was to determine the effects of feeding increasing levels of fresh forage (FF) as a proportion of total dry matter intake (DMI) on nutrient intake, rumen digestion, nutrient utilization, and productive performance of total mixed ration (TMR)-fed cows. Twelve dairy cows (90 ± 22 d in milk, 523 ± 88 kg of body weight, 7,908 ± 719 kg of milk production in the previous lactation) were housed in individual tiestalls and assigned to treatments according to a 3 × 3 Latin square design replicated 4 times. Treatments were 100% TMR (T100), 75% TMR plus 25% FF (T75), and 50% TMR plus 50% FF (T50). The experiment lasted 60 d, divided into 3 periods of 20 d each; the first 12 d of each period were used for diet adaptation and the last 8 d for data collection. The TMR (18.1% crude protein, 24.6% acid detergent fiber) and FF (Lolium multiflorum; 15.1% crude protein, 24.1% acid detergent fiber) were prepared and cut daily and offered to each cow individually. The highest DMI was reached in T100 and T75, which was reflected in greater intake of the different nutrients than T50. No differences were detected in the apparent total digestibility of the nutrients, mean ruminal pH, and total volatile fatty acid concentrations among treatments. Cows in T50 resulted in the lowest ruminal N-NH3 concentration and the lowest microbial N flow to the duodenum. Milk yield was 8.5% higher from cows in T100 and T75 compared with T50, but we observed no differences for milk fat or milk protein yield among treatments. Milk fat of cows fed T50 had 8% more unsaturated fatty acids (FA) than that of cows fed T100, mostly because of a higher content of monounsaturated FA. Additionally, cows in T50 had a higher concentration of linoleic acid, vaccenic acid, and rumenic acid than T100. Meanwhile, the concentration of linoleic acid and vaccenic acid in cows fed T75 was higher than T100. The milk fat of the cows fed T50 and T75 had a lower n-6:n-3 ratio than T100. We concluded that including up to 29% of FF in the total DMI in combination with a TMR did not affect the intake or digestion of nutrients or the productive response in dairy cows and resulted in a higher concentration of desirable FA from a consumer's perspective.

Relationship Between the Content of Chlorinated Hydrocarbons and Fatty Acid Composition of Milk Fat

IntroductionReports that the presence of persistent organic pollutants in fat may affect fatty acid metabolism prompted this research aiming to study the relationship between the contents of γ-HCH and DDT, DDE, DDD, and ΣDDT, and fatty acid composition of milk fat.Material and MethodsThe material consisted of 50 samples of cow and mare milk, collected in 2015. Ludwicki’s and the Röse-Gottlieb and IDF Standard methods were used to prepare the samples. Statistical analyses were conducted using Statistica 12.0.ResultsThere was a negative correlation between the content of γ-HCH and C16:1, C17:1, C18:1c9, C18:1c9c12, and ΣMUFA in cow milk fat and C13:0, C14:0, and C10:1 in mare milk fat. A positive correlation was observed between γ-HCH and C6:0 to C12:0, C14:0, C18:1t16, and ΣSFA in cow milk fat, and between this compound and C14:0iso, C16:1, C17:1, C18:1c9,11, and ΣMUFA in mare milk fat. A negative correlation between the contents of ΣDDT and C16:1, C17:1, C18:1c9,11,13 and ΣMUFA in cow milk fat and C16:0iso, C17:0, and C18:3 in mare milk fat was noted. A positive correlation was found between the contents of ΣDDT and saturated and polyunsaturated fatty acids and ΣSFA and ΣPUFA in cow milk fat, and C18:2c9c12 in mare milk fat.ConclusionThe correlation between the content of selected organochlorine compounds and the composition of fatty acids in cow and mare milk fat indicates the strong influence of these environmental pollutants on the nutritional value of milk fat.

Relationship between the content of chlorinated hydrocarbons and fatty acid composition of milk fat

Abstract Introduction Reports that the presence of persistent organic pollutants in fat may affect fatty acid metabolism prompted this research aiming to study the relationship between the contents of γ-HCH and DDT, DDE, DDD, and ΣDDT, and fatty acid composition of milk fat. Material and Methods The material consisted of 50 samples of cow and mare milk, collected in 2015. Ludwicki’s and the Röse-Gottlieb and IDF Standard methods were used to prepare the samples. Statistical analyses were conducted using Statistica 12.0. Results There was a negative correlation between the content of γ-HCH and C16:1, C17:1, C18:1c9, C18:1c9c12, and ΣMUFA in cow milk fat and C13:0, C14:0, and C10:1 in mare milk fat. A positive correlation was observed between γ-HCH and C6:0 to C12:0, C14:0, C18:1t16, and ΣSFA in cow milk fat, and between this compound and C14:0iso, C16:1, C17:1, C18:1c9,11, and ΣMUFA in mare milk fat. A negative correlation between the contents of ΣDDT and C16:1, C17:1, C18:1c9,11,13 and ΣMUFA in cow milk fat and C16:0iso, C17:0, and C18:3 in mare milk fat was noted. A positive correlation was found between the contents of ΣDDT and saturated and polyunsaturated fatty acids and ΣSFA and ΣPUFA in cow milk fat, and C18:2c9c12 in mare milk fat. Conclusion The correlation between the content of selected organochlorine compounds and the composition of fatty acids in cow and mare milk fat indicates the strong influence of these environmental pollutants on the nutritional value of milk fat.

In vitro response to EPA, DPA, and DHA: Comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes.

The modulation of milk fat nutritional quality through fish oil supplementation seems to be largely explained by the action of n-3 very long chain polyunsaturated fatty acids (PUFA) on ruminal biohydrogenation (BH) of C18 fatty acids (FA). However, relationships among this action, disappearance of those PUFA in the rumen, and potential detrimental consequences on ruminal fermentation remain uncertain. This study compared the effect of 20:5n-3 (eicosapentaenoic acid; EPA), 22:5n-3 (docosapentaenoic acid; DPA), and 22:6n-3 (docosahexaenoic acid; DHA) on rumen fermentation and BH of C18 FA and was conducted simultaneously in cows and sheep to provide novel insights into interspecies differences. The trial was performed in vitro using batch cultures of rumen microorganisms with inocula collected from cannulated cows and ewes. The PUFA were added at a dose of 2% incubated dry matter, and treatment effects on ruminal C18 FA concentrations, PUFA disappearances, and fermentation parameters (gas production, ammonia and volatile FA concentrations, and dry matter and neutral detergent fiber disappearances) were examined after 24 h of incubation. A principal component analysis suggested that responses to PUFA treatments explained most of the variability; those of ruminant species were of lower relevance. Overall, EPA and DHA were equally effective for inhibiting the saturation of trans-11 18:1 to 18:0 and had a similar influence on ruminal fermentation in cows and sheep (e.g., reductions in gas production and acetate:propionate ratio). Nevertheless, DHA further promoted alternative BH pathways that lead to trans-10 18:1 accumulation, and EPA seemed to have specific effects on 18:3n-3 metabolism. Only minor variations attributable to DPA were observed in the studied parameters, suggesting a low contribution of this FA to the action of marine lipids. Although most changes due to the added PUFA were comparable in bovine and ovine, there were also relevant specificities, such as a stronger inhibition of 18:0 formation in cows and a greater increase in 18:3n-3 metabolites in sheep. No direct relationship between in vitro disappearance of the incubated PUFA and effect on BH (in particular, inhibition of the last step) was found in either cows or ewes, calling into question a putative link between extent of disappearance and toxicity for microbiota. Conversely, an association between the influence of these PUFA on ruminal lipid metabolism and fermentation may exist in both species. In vivo verification of these findings would be advisable.

Desventajas de la introducción de la leche de vaca en el primer años de vida

El costo de las fórmulas infantiles suele ser una de las principales razones por las que las madres prefieren el uso de leche entera de vaca en menores de un año; sin embargo, su empleo en niños de esa edad puede causar daños en la salud. El objetivo de este trabajo es revisar la evidencia científica sobre los riesgos y efectos adversos de alimentar con leche entera a los lactantes antes del primer año de vida. La leche entera de vaca no es una fuente significativa de ácido ascórbico y hierro; este hecho y los microsangrados intestinales que elevan considerablemente el riesgo de causar anemia. La leche de vaca tiene menor contenido de ácidos grasos esenciales que son necesarios para el neurodesarrollo y para la función visual de lactante; por esta razón la leche descremada es menos recomendable. El alto contenido de proteína y nutrimentos inorgánicos da una alta carga de solutos renales, la que en ciertos casos puede causar deshi- dratación. En ocasiones los lactantes pueden tener alergia a la proteína de la leche. Si bien hacen falta más estudios, existe un número creciente de evidencias que vinculan la introducción temprana de la leche de vaca con algunas enfermedades crónico-degenerativas en los niños tales como la diabetes y la obesidad.

Development of an improved extraction and HPLC method for the measurement of ascorbic acid in cows' milk from processing plants and retail outlets

SummaryAn improved extraction (2.5% HPO3, 5 mm dithiothreitol) and HPLC quantification methodology using a C–18 column at 35 °C and 0.1 m acetic acid (98%) and acetonitrile (2%) mobile phase was developed to quantify total ascorbic acid (AA) in commercial whole/semi‐skim/skim raw/pasteurised/UHT milk packaged in opaque bags, transparent plastic, cardboard and Tetra Brik™. AA content ranged from 0.21 to 10 and from 3.4 to 16 mg L−1 in milk from retail outlets and processing plants, respectively, and was higher in organic milk. For same processor/lot samples, pasteurised milk showed higher AA content than UHT milk. This was not true for retail outlets samples. AA content was similar for whole/semi‐skim and semi‐skim/skim milk, but not for whole/skim comparisons. Among UHT samples, the AA content trend was whole&lt;semi‐skim&lt;skim and lower for UHT milk in opaque plastic and Tetra Brik™ container. After 14 days at 4 °C in the dark, AA losses ranged 35–83% depending on milk type and preservation method with a higher AA retention in unopened containers.

Hypothesis: Is the Omega-3 PUFA Content of Milk Related to Type 1 Diabetes?

AbstractThe incidence of Type 1 Diabetes (T1D) has been increasing over the past 50 years. The geographical variation in the incidence of T1D varies 400 fold from a low rate in China to a very high incidence in Finland, Sardinia and Yemenite Jews in Israel. The reason behind these epidemiologic findings is unclear at this time and further elucidating the factors related to this intriguing epidemiology of T1D can help us better understand certain risk factors, the pathogenesis of the disease, and help reduce the incidence of T1D. We hypothesize that the variation of the omega-3 polyunsaturated fatty acid (PUFA) content of cow’s milk and human breast milk may play a very important role in the development of Type 1 Diabetes in genetically predisposed individuals and may also be an important confounder in many previously published and ongoing studies. Interestingly, the amount of omega 3 fatty acids in cow’s milk and human breast milk has been shown to vary widely depending on the diet of the cows and nursing mothers. This hypothesis could possibly help explain the conflicting results from various studies regarding cow’s milk exposure, breastfeeding and T1D and may also help explain some of the findings related to other environmental risk factors for the development of T1D through the beneficial effects of omega-3 fatty acids on this autoimmune disease.