Acid Detergent Insoluble Protein Research Articles

Determination of feed yield and quality parameters of whole crop durum wheat (Triticum durum Desf.) biomass under agrivoltaic system

AbstractAgrivoltaics represents a key technology for reaching sustainable development goals by reducing the competition between land used for food, for feed, and for electricity. It has been demonstrated that Agrivoltaics can increase land productivity and play a role in the expansion of renewable energy production. This work aimed to study the yield and nutritional characteristics, as well as feeding value for ruminants of Durum wheat biomass grown under agrivoltaic. Two years of controlled experiments revealed that the reduction in light moderately limited wheat yields in the phenological phase of soft dough in standard agrivoltaic trackers (i.e. with a Ground Coverage Ratio (GCR) = 13%), otherwise under extended trackers (i.e. GCR = 41%), the yields was reduced compared to control in whole light. The digestible neutral detergent fiber evaluated after 24 h of in situ rumen incubations increased with shading, resulting in different ratios of acid detergent lignin. More shaded theses also had superior crude and soluble proteins, acid detergent-insoluble protein, acid and neutral detergent fiber than the control. The biomass in the shaded treatments showed a better Ca: P ratio for ruminant nutrition. These characteristics are strategic in forage production, allowing a more flexible harvesting strategy. This additional contribution of the nutritional characteristics of Durum wheat produced on Agrivoltaic Systems could allow a better inclusion of the different types in diets and better management of silage or hay harvesting sites. These results might be helpful in improving biomass production and give valuable information on Durum Wheat under the Agrivoltaic System.

Synergistic effects of ferulic acid esterase-producing lactic acid bacteria, cellulase and xylanase on the fermentation characteristics, fibre and nitrogen components and microbial community structure of Broussonetia papyrifera during ensiling.

The high fibre content of whole plants of Broussonetia papyrifera limits its efficient utilization. Ferulic acid esterase (FAE), in combination with xylanase, can effectively cleave the lignin-carbohydrate complex, promoting the function of cellulase. However, little is known about the impact of these additives on silage. To effectively utilize natural woody plant resources, FAE-producing Lactiplantibacillus plantarum RO395, xylanase (XY) and cellulase (CE) were used to investigate the dynamic fermentation characteristics, fibre and nitrogen components and microbial community structure during B. papyrifera ensiling. Broussonetia papyrifera was either not treated (CK) or treated with FAE-producing lactic acid bacteria (LP), CE, XY, LP + CE, LP + XY or LP + CE + XY for 3, 7, 15, 30 or 60 days, respectively. In comparison with those in the CK treatment, the L. plantarum and enzyme treatments (LP + CE, LP + XY and LP + XY + CE), especially the LP + XY + CE treatment, significantly increased the lactic acid concentration and decreased the pH and the contents of acid detergent insoluble protein and NH3 -N (P < 0.05). Enzyme addition improved the degradation efficiency of lignocellulose, and a synergistic effect was observed after enzyme treatment in combination with LP; in addition, the lowest acid detergent fibre, neutral detergent fibre, hemicellulose and cellulose contents were detected after the LP + CE + XY treatment (P < 0.05). Moreover, CE, XY and LP additions significantly improved the microbial community structure, increased the relative abundance of Lactiplantibacillus and Firmicutes, and effectively inhibited undesirable bacterial (Enterobacter) growth during ensiling. FAE-producing L. plantarum and the two tested enzymes exhibited synergistic effects on improving the quality of silage, which indicates that this combination can serve as an efficient method for improved B. papyrifera silage utilization. © 2023 Society of Chemical Industry.

Solid-State Fermentation with White Rot Fungi (Pleurotus Species) Improves the Chemical Composition of Highland Barley Straw as a Ruminant Feed and Enhances In Vitro Rumen Digestibility.

Lignin degradation is important for enhancing the digestibility and improving the nutritive quality of ruminant feeds. White rot fungi are well known for their bioconversion of lignocellulosic biomass. The objective of this paper was to evaluate whether Lentinus sajor-caju, Pleurotus ostreatus, Phyllotopsis rhodophylla, Pleurotus djamor, Pleurotus eryngii, and Pleurotus citrinopileatus treatments altered the chemical compositions of highland barley straw constituents and enhanced their nutritional value as a ruminant feed. All white rot fungi significantly increased the relative crude protein (CP), ethyl ether extract (EE), starch, soluble protein (SP), and non-protein nitrogen (NPN) contents but decreased the ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and acid detergent insoluble protein (ADFIP) contents. In addition, L. sajor-caju treatment increased (p < 0.001) the levels of PA, PB2, PB3, CA, CB1, CB2, and CNSC, but reduced (p < 0.001) the PC and CC in the solid-state fermentation of highland barley straw. Maximum ligninlysis (50.19%) was optimally produced in the presence of 1.53% glucose and 2.29% urea at 22.72 ℃. The in vitro dry matter digestibility and total volatile fatty acid concentrations of fermented highland barley straw, as well as the fermentability, were optimized and improved with L. sajor-caju, which degraded the lignocellulose and improved the nutritional value of highland barley straw as a ruminant feed.

Ensiling and thermic treatment effects on ruminal carbohydrate fermentation and post-ruminal crude protein concentration in partial-crop peas and faba beans

This study was conducted to examine effects of ensiling, toasting or ensiling plus toasting in partial-crop field peas and faba beans harvested each with 375 g dry matter/kg (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie (BBCH) scale 79 and 81, respectively) on gas production and post-ruminal crude protein (PRCP) concentration byin vitro incubation in ruminal fluid batch-cultures. The silages made from partial-crop field peas and faba beans (Rostock Model Silages) had a pH of 4.3 and 4.6, respectively, and were not typically lactic acid dominated. The silages remained stable after opening for 100 h (peas) and 168 h (faba beans). Toasting was simulated in a drying oven at 160 °C for 60 min. Post-incubation pH and gas accumulation profiles were little affected by treatment. Ensiling did not alter effective PRCP. Toasting and ensiling plus toasting increased effective PRCP up to 25 and 20%-points in peas and up to 35 and 11%-points in faba beans, respectively. Ensiling increased non-protein nitrogen and soluble protein concentration, whilst toasting decreased soluble protein. Significant correlations existed between protein fraction B3 (neutral detergent-insoluble protein) and effective PRCP (r≥0.84;P&lt;0.05) and fraction C (acid detergent-insoluble protein) and effective PRCP (r≥0.79;P&lt;0.05). Ensiling and toasting both decreased arginine and lysine levels. It was concluded that partial-crop peas and faba beans with BBCH 79 and 81, respectively, can provide readily available nutrients and high-quality fibre in the residual plant. However, preserving by ensiling required balance between the reduction of non-protein nitrogen and fermentability characteristics. Toasting reduced protein solubility and increased PRCP, but it was not clear if PRCP was usable for ruminants or was partially bound into Maillard polymers.

84 Relationships between feed protein fractions and the hindgut microbiome in the exercising horse

The effect of dietary protein on the microbiome in the horse has limited data. In this study, 16 horses (averaging 606 kg BW) were used to evaluate the effect of protein source (forage, F, or forage and concentrate, FC) on hindgut microbiome. Horses were fed grass hay (timothy/orchardgrass). Those in the FC groups were also fed concentrate (Legends Growth, Southern States) at a ratio of 30:70 concentrate to forage. Horses consumed 2.44, 2.26 and 3.19 g CP/kg BW/d respectively for the F, FC(low and high CP) groups. Diets were fed for 14 wk. Horses participated in light exercise (1–2 h/d, 5 d/wk). Forage, concentrate and fecal samples were collected every 2 wk. Forage and concentrate samples were analyzed for CP, ADICP (acid detergent insoluble protein) and NDICP (neutral detergent insoluble protein). Fecal samples were analyzed for bacterial community abundance (PCR and sequencing). Statistical analysis of fecal communities was done using the Krustal-Wallis analysis and correlations determined using Slidewrite Plus. At the phylum level, there were positive linear correlations between the amount of daily CP from forage (g/kg BW/d) for both the F and FC groups for Proteobacteria (r = 0.54, P < 0.05), and Firmicutes (r = 0.55 and 0.7 respectively, P < 0.05). There was a positive linear correlation between ADICP and Proteobacteria for the FC groups (r = 0.8, P < 0.05). There was a linear correlation with Firmicutes (r = 0.59 and 0.82 respectively, P < 0.05), and quadratic correlations withBacteroidetes (r = 0.58 and 0.8 respectively, P < 0.05) and Tenericutes (r = 0.59 and 0.92 respectively, P < 0.05) for the FC and F groups with abundances increasing then decreasing for Bacteroides and vice versa for Tenericutes. For NDICP, there was a positive linear correlation with Firmicutes (r = 0.62, P < 0.05) and Proteobacteria (r = 0.52, P < 0.05) for the FC groups and for Fibrobacteres (r = 0.95 and 0.89 respectively, P < 0.05), while Bacteroides (r = 0.57 and 0.8 respectively, P < 0.05) as well as Tenericutes (r = 0.62 and 0.94 respectively, P < 0.05) had quadratic correlations with abundances decreasing then increasing for Bacteroides and vice versa for Tenericutes for FC and F groups. Nitrogen is needed in the hindgut for the microbiome and the changes observed in the Firmicutes and Fibrobacteres support this. In swine, excess dietary CP changed the microbiome that negatively affect gut health. This study suggests N has importance to the hindgut microbiome but also that excessive protein may impact the microbiome as well. Changes in the microbiome in horses due to diet which may affect fiber digestibility, gut and host health warrant further investigation in the horse.

Degradability of Vicia ervilia Grain Using In Situ and CNCPS Methods, and Model-Based Analysis of Its Ruminal Degradation

Using nylon bag techniques, Cornell net carbohydrates and protein systems (CNCPS), and scanning electron microscopy, the authors examined the digestibility and structure of Vicia ervilia (ervil, bitter vetch) after steam flaking, roasting, and microwave processing. During the in situ technique, the samples were incubated at 0, 2, 4, 6, 8, 12, 16, 24, 36, and 48 h. For the description of the ruminal DM (dry matter) and CP (crude protein) degradation kinetics of treated and untreated Vicia ervilia, different models were selected as the best fit for the dry matter (DM) and crude protein (CP) degradation parameters of steam flaked samples. The results showed that both the steam flaking and microwave treatment samples contained high levels of non-protein nitrogen and buffer soluble protein, respectively. In comparison with steam flaking and microwave treatment, roasting decreased and increased the buffer soluble protein and neutral detergent insoluble protein, respectively. The control treatments showed the highest levels of neutral detergent soluble protein and the lowest levels of acid detergent soluble protein. Moreover, steam flaking and roasting decreased and increased the amount of acid detergent insoluble protein, respectively. When using dry heat (microwave and roasting), the acid detergent soluble and insoluble protein fractions were increased. As a result of this experiment, the nitrogen fractions were altered using heat processing. Hence, protein fermented feed and ruminal fermentation conditions can be expressed using these results.

Effects of combined nitrogen and phosphorus application on protein fractions and nonstructural carbohydrate of alfalfa.

Nitrogen (N) and phosphorus (P) fertilization significantly affect alfalfa production and chemical composition; however, the effect of combined N and P application on protein fractions and the nonstructural carbohydrate content of alfalfa is not fully understood. This two-year study investigated the effects of N and P fertilization on the protein fractions, nonstructural carbohydrates (NSC), and alfalfa hay yield. Field experiments were carried out using two nitrogen application rates (N60, 60 and N120, 120 kg N ha - 1) and four phosphorus application rates (P0, 0; P50, 50; P100, 100; and P150, 150 kg P ha - 1), total 8 treatment (N60P0, N60P50, N60P100, N60P150, N120P0, N120P50, N120P100 and N120P150). Alfalfa seeds were sown in the spring of 2019, uniformly managed for alfalfa establishment, and tested in the spring of 2021-2022. Results indicated that P fertilization significantly increased the hay yield (3.07-13.43% ranges), crude protein (6.79-9.54%), non-protein nitrogen of crude protein (fraction A) (4.09-6.40%), and NSC content (11.00-19.40%) of alfalfa under the same treatment of N application (p < 0.05), whereas non-degradable protein (fraction C) decreased significantly (6.85-13.30%, p < 0.05). Moreover, increasing N application resulted in a linear increase the content of non-protein N (NPN) (4.56-14.09%), soluble protein (SOLP) (3.48-9.70%), and neutral detergent-insoluble protein (NDIP) (2.75-5.89%) (p < 0.05), whereas acid detergent-insoluble protein (ADIP) content was significantly decreased (0.56-5.06%, p < 0.05). The regression equations for nitrogen and phosphorus application indicated a quadratic relationship between yield and forage nutritive values. Meanwhile, the comprehensive evaluation scores of NSC, nitrogen distribution, protein fractions, and hay yield by principal component analysis (PCA) revealed that the N120P100 treatment had the highest score. Overall, 120 kg N ha - 1 coupled with 100 kg P ha - 1 (N120P100) promoted the growth and development of perennial alfalfa, increased soluble nitrogen compounds and total carbohydrate content, and reduced protein degradation, thus improving the alfalfa hay yield and nutritional quality.

Gernik (Gacer, Kavılca) Buğdayı (Triticum dicoccum) ve Yan Ürünlerinin Ruminant Hayvanlar İçin Yem Değeri

Ancient wheat species attracts more attention recently due to their health benefits and suitability for organic farming. With this attention, the possibilities of using ancient wheat species and especially their by-products in animal nutrition are emerging. Unlike modern wheat varieties, emmer is known as one of the ancient wheat varieties, has hull covering its grains. Approximately 70% of the total plant weight consists of hulls and stalks. Looking at the literature, limited studies has been conducted regarding the evaluation of the hull and stalk parts of emmer wheat as feed. Herein, this study aims to determine the nutritional values of emmer wheat and its by-products. The feed value of the plant was analyzed in five parts (hulled grain, stalk of plant, hull, naked grain and flour). For each part, dry matter (DM), crude protein (CP), ether exract (EE), crude ash (CA), starch, crude fiber (CF), acid detergent fiber (ADF), neutral detergent fiber (NDF), acid detergent insoluble crude protein (ADICP), neutral detergent insoluble crude protein (NDICP), lignin and mineral analyzes were made. In addition, non-fiber carbohydrate (NFC), digestible dry matter (DDM), dry matter intake by animal body weight (DMIBW), total digestible nutriton (TDN1X), net energy lactation (NEL3X), metabolic energy (ME), net energy maintanence (NEM), net energy gain (NEG), energy and digestibility calculations were performed. The protein content of emmer grain was observed higher than that of modern wheat. ADF and NDF values of the emmer wheat stalks are lower than modern wheat stalks and therefore have better digestibility values. Additionally, the higher resistance to starch digestion compared to modern wheat varieties may be a reason for preference in ruminant feeding.

Nutritional Values of Industrial Hemp Byproducts for Dairy Cattle.

The objective of this experiment was to explore the nutritional components of industrial hemp byproducts (industrial hemp ethanol extraction byproduct, IHEEB; industrial hemp stalk, IHS; industrial hemp seed meal, IHSM; industrial hemp oil filter residue, IHOFR) and provide theoretical support for the application of industrial hemp byproducts in dairy cattle production. This experiment used a combination of a wet chemical method with Cornell Net Carbohydrate and Protein System, in situ nylon bag technique, and three-step in vitro method to compare the chemical composition, carbohydrate and protein composition, in situ ruminal degradability and intestinal digestibility of industrial hemp byproducts and conventional feeds (alfalfa hay, AH; soybean meal, SBM). Available energy values were estimated based on the National Academies of Sciences, Engineering, and Medicine. The results showed that the nutritional composition of different feeds varied greatly. The two types of IHEEB were enriched with ash, crude protein (CP), neutral detergent fiber (NDF), and calcium, while the contents of neutral detergent insoluble crude protein, acid detergent insoluble crude protein, and acid detergent lignin were higher. As a result, the non-degradable carbohydrate and protein components were higher, and the effective degradation rate of rumen dry matter and protein was lower. IHS contains higher non-protein nitrogen and NDF, which enables it to provide more CP rumen effective degradation rate and carbohydrates, but the high acid detergent fiber also limits its application. IHSM possesses 296 g/kg CP and high rumen undegradable protein and intestinal digested protein, which can provide rumen bypass protein in dairy cows, making it a potentially good protein source. IHOFR had higher ether extract, rumen available protein degradation rate, and total tract digested protein, which can provide more energy and easily degradable protein for lactating cows. The available energy value of IHEEB and IHS was lower than AH, while SBM is between IHFOR and IHSM. In addition, the tetrahydrocannabinol of three industrial hemp byproducts that have not been assessed by the European Food Safety Authority (EFSA) was tested to evaluate their safety, and all of them were less than the limit set by ESFA. In conclusion, industrial hemp byproducts can be considered for inclusion in dietary formulations as unconventional feed sources for dairy cattle, but the purpose of use needs to be properly considered.

Effects of intrinsic tannins on proteolysis dynamics, protease activity, and metabolome during sainfoin ensiling.

Condensed tannins (CT) from sainfoin have a high capacity to inhibit proteolysis. A previous study reported that CT from sainfoin can inhibit lactic acid bacteria activity and decrease ammonium-nitrogen (N) content during sainfoin ensiling; however, no study has focused on the metabolome of ensiled sainfoin. The objective of the present study was to investigate the effects of CT [following supplementation of deactivated CT with polyethylene glycol (PEG)] on protease activity, keystone bacteria, and metabolome during sainfoin ensiling. According to the results, PEG amendment increased non-protein N, amino acid, and soluble protein contents significantly (in the 49.08–59.41, 116.01–64.22, and 23.5–41.94% ranges, respectively, p < 0.05) during ensiling, whereas neutral detergent-insoluble protein and acid detergent-insoluble protein were decreased significantly (in the 55.98–64.71 and 36.58–57.55% ranges, respectively, p < 0.05). PEG supplementation increased aminopeptidase and acid protease activity after 3 days of ensiling (p < 0.05) and increased carboxypeptidase activity during the entire ensiling process (p < 0.05). The keystone bacteria changed following PEG addition (Stenotrophomonas, Pantoea, and Cellulosimicrobium in the control vs. Microbacterium, Enterococcus, and Brevundimonas in the PEG-treated group). In total, 510 metabolites were identified after 60 days of sainfoin ensiling, with 33 metabolites annotated in the Kyoto Encyclopedia of Genes and Genomes database. Among the metabolites, phospholipids were the most abundant (72.7% of 33 metabolites). In addition, 10 upregulated and 23 downregulated metabolites were identified in the PEG-treated group when compared with the control group, after 60 days of ensiling (p < 0.05). Pediococcus (correlated with 20 metabolites, R2 > 0.88, p < 0.05) and Lactobacillus (correlated with 16 metabolites, R2 > 0.88, p < 0.05) were the bacteria most correlated with metabolites. The results suggested antagonistic effects between Lactobacillus and Pediococcus during ensiling. The decreased proteolysis during sainfoin ensiling was mainly attributed to the inhibition of protease activity by CT, particularly carboxypeptidase activity. In addition, proteolysis decreased partly due to CT inhibiting Pediococcus activity during ensiling, with Pediococcus being significantly and positively correlated with dopamine after 60 days of ensiling (R2 = 0.8857, p < 0.05).

In Vitro Measurements of True Digestibility and Products of Digestion Using Multiple Cultivars of Non-Extracted and CBD-Extracted Industrial Hemp Biomass (Cannabis sativa)

Since 2018, the growth of industrial hemp (Cannabis sativa) for extraction of cannabidiol (CBD) oil has increased in popularity. By-products resulting from the extraction of the oil have become more available but remain largely unutilized due to their novelty and current restrictions on animal feeding. One potential use for the leftover inflorescence may be as a feedstuff, but reports examining its nutrient quality are limited. Therefore, experiments were conducted to evaluate 9 cultivars of non-extracted and 9 separate cultivars of CBD-extracted foliage. Ground alfalfa cubes were included in experiments as a control. All samples were analyzed for ash, ether extract, crude protein, lignin, ash-free neutral detergent fiber, acid detergent insoluble crude protein, and neutral detergent insoluble crude protein. Total digestible nutrients (TDN) were calculated using summative equations. In vitro true digestibility (IVTD) was determined using a filter bag technique and 48-h digestion period. Cumulative gas production was measured in batch culture over a 48-h period, with methane and ammonia production, volatile fatty acid (VFA) formation, and dry matter disappearance (DMD) measured following this period. Substrates for gas production measurements were composed of the hemp sample, rye silage, and ground corn mixed at a 30:35:35 ratio. Grind size (0.5 vs. 2.0 mm) was evaluated using non-extracted hemp and did not influence IVTD. Nutrient composition ranges of non- and CBD-extracted samples were similar, except for a numerically higher mean ether extract (17.66% vs. 9.29%) and TDN (73.01% vs. 54.03%) in non-extracted hemp. None of the measured nutrients for alfalfa cubes fell within the observed concentration ranges of either hemp group, but TDN was numerically similar to that of CBD-extracted hemp. IVTD was numerically similar between both hemp groups and alfalfa cubes. In comparison to alfalfa cubes, VFA (both concentration and molar proportions), ammonia concentrations, pH, DMD, methane, and total gas production were numerically similar with concurrently run non- and CBD-extracted hemp groups. The results of this study serve as a foundation from which a larger database of nutrient component values for hemp may be compiled. Such databases are necessary to fully evaluate the feasibility of a feedstuff for inclusion in animal diets.

Chemical composition and fatty acid profile of a seedpropagated elephant grass genotype, ensiled at different regrowth ages

This study aimed to evaluate the chemical composition and fatty acid (FA) profile of forage and silage of a seed-propagated elephant grass genotype called “PCEA” harvested at 75, 90, 105 and 120 days of regrowth. A randomized block design with five replications was used. The results were analyzed by mixed models (P &lt; 0.05) that included treatment (regrowth age) as a fixed effect and block as a random effect. Linear and quadratic effects of the treatments were analyzed using orthogonal contrasts. There was linear increase on total dry matter (DM) forage production and linear decrease on leaf:stem ratio as a function of the increase in regrowth age (P &lt; 0.05). In response to the advance of regrowth age, “PCEA” forage and silage showed linear decreases on crude protein (CP) content (g kg-1 DM) and in vitro DM digestibility (g kg-1); and linear increases on DM (g kg-1), lignin (g kg-1 DM), and acid detergent insoluble protein (%CP) contents (P &lt; 0.05). All “PCEA” silages had DM content &lt; 200 g kg-1, pH &gt; 4.0, and ammonia N content &gt; 10% total N. In response to the advance of regrowth age, linear decreases (P &lt; 0.05) were observed on forage and silage linoleic, α-linolenic, and total FA contents (g kg-1 DM). From 75 to 120 days of regrowth, the seed-propagated elephant grass genotype “PCEA” presents forage and silage with low nutritional quality for feeding dairy cattle, moderate to high linoleic acid contents, and moderate to low α-linolenic acid contents. The “PCEA” forage and silage obtained from 75 to 120 days of regrowth presents low potential for production of milk naturally enriched with bioactive FAs beneficial to human health.

Nutritional value of Tifton 85 grass haylage under different layers of polyethylene film and storage times

Forage conservation processes are fundamentally aimed at maintaining the nutritional quality of forages so that they can overcome the low availability and quality of roughage feed in unfavorable times. The objective of this study was to examine the effect of the number of layers of polyethylene film and storage time on the nutritional value of haylage of Tifton 85 grass (Cynodon spp cv. Tifton 85). Four and six layers of milky white polyethylene film were used in the wrapping of silage bales with a capacity of 400 kg (100 and 150 μm in total thickness, respectively), for four different storage times (at baling and 30, 60, and 90 days). The experiment was laid out in a randomized-block design with four replicates, in a split-plot arrangement in time, in which the number of layers of polyethylene film that wrapped the haylage bales constituted the plots and the storage times the sub-plots. Neutral detergent insoluble protein (NDIP) levels were higher in the haylage wrapped with four layers of polyethylene film (35.8 g kg-1 CP). Storage time affected the neutral detergent fiber (NDF), acid detergent fiber (ADF), mineral matter (MM), NDIP, and lignin contents and in vitro dry matter digestibility (IVDMD). The ADF and lignin contents increased linearly, whereas IVDMD decreased linearly. Mineral matter showed a negative quadratic response. The NDIP and NDF contents exhibited a positive quadratic response. Storage time did not influence the dry matter (550.3 g kg-1), crude protein (102.3 g kg-1 DM), or acid detergent insoluble protein (28.9 g kg-1 CP) contents or in vitro NDF digestibility (582.1 g kg-1 DM). In conclusion, the number of layers of polyethylene film does not influence the nutritional value of Tifton 85 grass haylage. Storage for 90 days reduces the nutritional value of Tifton 85 grass haylage.

A novel sub-pilot-scale direct-contact ultrasonic dehydration technology for sustainable production of distillers dried grains (DDG)

DDG is a major source of protein, calcium, phosphorus, and sulfur is arguably the most important byproduct of the bioethanol industry with increasing demand over the past few years. Reducing energy consumption in the DDG production process and energy recovery from DDG is vital for sustainable bioethanol productions. In this paper, a novel direct-contact multi-frequency, multimode, and modulated (MMM) ultrasonic dryer (US) was developed for the first time and has been applied in dehydration of wet distillers’ grain (WDG). Ultrasonic drying (US) was combined with a convective airflow (HA) at different temperatures of 25 (room temperature), 50 and 70 °C to evaluate the impact of US, HA, and US + HA on drying kinetics, activation energy, chemical compositions, microstructure, and color of DDG. Semi-empirical kinetic models were developed and evaluating drying performances showed that the application of ultrasound significantly enhanced the drying rate and decreased the drying time (by 46%), especially at low drying temperatures. The activation energy for moisture removal in the presence of ultrasound was about 50% of that without ultrasound. The final dried distillers' grains product processed by ultrasonic drying had a brighter color, a higher available protein, a higher digestible protein (the lowest acid detergent insoluble crude protein), and a better surface profile with no compromise on minerals and fiber contents.

Effects of different moist orange pulp inclusions in the corn grain rehydration for silage production on chemical composition, fermentation, aerobic stability, microbiological profile, and losses.

This study evaluated the effects of different moist orange pulp (MOP) inclusions in corn grain rehydration for silage production as a strategy to store and use MOP and whether these inclusions result in adequate fermentation and aerobic stability (AE) and acceptable losses. Ground corn grain and MOP were weighed separately and mixed to obtain MOP inclusions of 21%, 34%, or 42%. The control treatment was obtained with corn rehydrated with distilled water and MOP inclusion of 0%, with five laboratory silos (15-L buckets) per treatment. Acid detergent fiber, acid detergent insoluble protein, acid detergent lignin, lactic acid, acetic acid, and ethanol contents increased linearly with increasing MOP inclusion, whereas starch, in vitro dry matter (DM) digestibility and butyric acid contents and pH decreased linearly. NH3 -N/TN content reduced quadratically and was minimal with 8.34 g/kg DM at 14% MOP inclusion. Total DM losses increased quadratically with maximal losses of 4.26% DM at 25% MOP inclusion. AE reduced by 2.8h for each 1% increase in MOP inclusion. Rehydration of corn grain with MOP results in an adequate fermentation process. The inclusion of 34% MOP was the best because DM losses were lower and AE was not drastically reduced.

​​In vitro Evaluation and Methane Production Potential of Rice Gluten Meal based Concentrate Feeds in Buffalo Inoculum

Background: There is shortage of conventional feedstuffs to feed livestock in the country. Thus, this study was intended to evaluate the chemical composition and in vitro nutrient utilization of concentrate feeds containing graded levels of rice gluten meal (RGM). Methods: Five concentrate feeds containing graded levels of RGM at 0, 6.25, 12.50, 18.75 and 25% replacing soybean meal @ 0, 25, 50, 75 and 100% on w/w basis were prepared and designated as conc-1, conc-2, conc-3,conc-4 and conc-5. In vitro evaluation of feeds was carried out by in vitro gas production technique. Data were analysed by one way ANOVA using SPSS (2012) version 21. Result: The total ash, acid detergent fibre (ADF) and acid detergent lignin (ADL) content decreased while organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), hemicellulose, acid detergent insoluble crude protein (ADICP), neutral detergent insoluble crude protein (NDICP) and total carbohydrate (TCHO) content increased with increase in the inclusion of RGM in the concentrate feeds. The OM digestibility was lower (P less than 0.05) in concentrate feeds 3, 4 and 5 than concentrate feed 1 and 2. Microbial mass production (MMP), efficiency of MMP and ME availability were not affected by including RGM in feeds. NH3-N showed a declining trend with increasing level of RGM in concentrate feeds. The relative proportion of acetate decreased (P less than 0.05) while that of propionate increased (P less than 0.05) with increasing level of RGM in the concentrate feeds. Methane production varied non-significantly among the concentrate feeds. The fermentation efficiency was higher (P less than 0.05) in concentrate 4 and 5 than other concentrates.

Feed-value of post-harvest quinoa plant sections grown by different cultural applications

The aim of the study, the grains of the quinoa plant grown in the Kahramanmaraş region, Turkey, which was sown at different times (March 26, April 2, 13 and 26, and May 11) in various row spacing applications (20, 40 and 60 cm) was separated, and the feed-worthiness of the remaining plant sections were analyzed. The study findings demonstrated that crude protein content was 9.70-19.30%, dry matter ratio was 86.18-88.20%, acid detergent fiber content was 42.95-55.95%, neutral detergent fiber content was 51.23-64.27%, acid detergent insoluble protein content was 0.88-1.37%, digestible dry matter content was 45.3-55.4%, drymatter intake rate was 1.87-2.34%, relative feed value was 66.88-96.49%, and quality standard value varied between III and V. Mineral content was determined as follows: Ca: 0.96-1.96%, K: 1.47-2.08%, Mg: 0.17-0.74%, P: 0.18-0.37%, Tetany: 1.51-1.99, milk fever: 3.69-9.49. It was determined that the sowing time with the highest feed values for quinoa straw was May 11, while the ideal row spacing was 40 cm. Thus, a feed with higher protein and mineral content but low indigestible nutrient content could be obtained. However, it was concluded that it would be more adequate to employ the feed in composite form with other feed plants for feed quality.

Levels of acid detergent insoluble protein in grasses and feeds made from them

The purpose of this research is to assess the level of thermal damage by determining the content of acid detergent insoluble crude protein (ADICP) in perennial cereal forage grasses Bromus inermis, Festuca pratensis, Phleum, depending on the phase of their vegetation (at the beginning of shooting, during the earing and flowering phases), as well as in silage and haylage prepared from them during the same phases. Samples for analyzes were dried at the temperature of 60-65 °C. Nitrogen of ADICP was determined in the residue of ADF (acid detergent fiber) obtained by filtering a solution of acid detergent through a paper filter. With the growth of grasses, an increase in the content of ADF in them was observed, followed by an increase in ADICP in Phleum and especially in Festuca pratensis which was characterized by higher rates of ADF accumulation. In Bromus inermis, the increase in ADF did not lead to an increase in the concentration of ADICP in the grass due to a decrease of its portion in ADF. The content of ADICP in green grass ranged from 0.85 to 1.58 % in dry matter, ADF ‒ from 27.0 to 45.8 %, no correlation was found between them. Conservation of grasses in all phases of their growth caused an increase in the content of ADF compared to the original grass, but the mass fraction of ADICP in the dry matter of silage and haylage was not higher than in the original grass, due to a lower mass fraction of ADICP in the ADF compared to grasses. In this regard, it is noted that if the technology of harvesting silage and haylage is observed, there is no increase in the level of thermal damage to these feeds. In this regard, it is noted, that when the preparation of silage and haylage is carried out according to the required technology, there is no increase in the level of thermal damage to these feeds. There is close correlation between the percentage of ADICP in CP (crude protein) and the contents of CP. The correlation coefficients were -0.83; -0.88 and -0.92 for grasses, silage and haylage, respectively. The lowest percentage of ADICP in CP was observed in the early phases of grass growth. In this regard, there is a need to harvest them in earlier growth phases, since the content of CP decreases with growth and the proportion of indigestible protein in it increases. If there are organoleptic signs of thermal damage to feeds given in the article, it is recommended to make an amendment to CP content on the basis of the result of the analysis for the content of ADICP.

Chitosan and microbial inoculants in whole-plant soybean silage

AbstractWhole-plant soybean silage (WPSS) is a potential high-protein roughage source for ruminant diets. However, WPSS can be difficult to ensile and fermentation is a challenge. This study was conducted to evaluate the effect of chitosan and microbial inoculants on fermentation profile, fermentation losses, chemical composition, and in vitro degradation of WPSS. Forty experimental silos (PVC tubing with 28 cm i.d. and 25 cm height) were produced. Soybean plants from 10 plots were ensiled in a completely randomized block design to evaluate the following treatments: (1) control (CON): WPSS without additives; (2) chitosan (CHI): WPSS additive with 6 g/kg DM of chitosan; (3) LBB: WPSS treated with 5.0 × 107 colony-forming units (CFU) of Lactobacillus buchneri (NCIM 40788) per kg of fresh matter and (4) LPP: WPSS treated with 1.6 × 108 CFU of Lactobacillus plantarum and 1.6 × 108 CFU of Pediococcus acidilactici per kg of fresh matter. Silos were opened 120 days after ensiling. Microbial inoculants reduced silage pH, whereas LPP-treated silos showed the lowest concentration of NH3-N, ethanol, butyric, acetic, branched-chain, and propionic organic acids. LBB-treatment decreased lactic acid bacteria (LAB) count relative to other treatments, and LPP-treatment showed the lowest fermentation losses, improving dry matter (DM) recovery. Relative to other treatments, LPP increased silage DM, organic matter, and decreased acid detergent insoluble crude protein (CP), improving DM and neutral detergent fibre in vitro degradation. Treatments showed no effect on silage aerobic stability. Thus, LPP-treatment improves fermentation profile, reduces fermentation losses, and increases the nutritional value of WPSS.

Chemical composition, fractionation of carbohydrates and nitrogen compounds, ruminal degradation kinetics, and in vitro gas production of cactus pear genotypes

Abstract The aim of this study was to evaluate the chemical composition, fractionation of carbohydrates and nitrogen compounds, degradation parameters, and in vitro ruminal fermentation of cactus pear genotypes. The experiment was conducted in a completely randomized design with 4 cactus pear genotypes and 4 replicates. The evaluated cactus pear genotypes were: Miúda (Nopalea cochenillifera Salm Dyck), IPA Sertânia (Nopalea cochenillifera), Gigante (Opuntia ficus indica Mill), and Orelha de Elefante Mexicana (Opuntia stricta Haw), all in natura. Samples were randomly collected at different points in the experimental area. Whole plants were collected 24 months after field crop establishment. N. cochenillifera Salm Dyck presented the highest dry matter, acid detergent insoluble protein, non-fibrous carbohydrate, total digestible nutrients, digestible energy, total carbohydrates, and fractions A + B1 (P&lt;0.05), while presenting lower neutral detergent fiber corrected for ash and protein, acid detergent fiber, and cellulose in relation to the other genotypes studied (P&lt;0.05). The in vitro true digestibility of neutral detergent fiber was high for the genotypes N. cochenillifera Salm Dyck and N. cochenillifera Dyck, which also presented high in vitro total gas production (P&lt;0.05). The cactus pear genotypes show adequate chemical characteristics to be composed part of diets offered to ruminants. However, supplementation is necessary to increase the dry matter and fiber contents. The Nopalea cochenillifera Salm Dyck genotype presented the highest proportions of total digestible nutrients, non-fibrous carbohydrates, non-protein nitrogen, unavailable nitrogen fraction total, and high gas production in relation to the other analyzed genotypes 24 months after field crop establishment.