Accutrend Plus Research Articles

Метаболічна характеристика організму спортсменок, які спеціалізуються в дзюдо, при виконанні навантаження анаеробного характеру

Judo is a physically demanding sport that requires a combination of strength, speed, agility, and endurance. While aerobic fitness is important for sustaining activity over a longer period of time, anaerobic fitness is also crucial for judo athletes, as the sport involves explosive movements and short bursts of intense activity. The purpose of the study: to evaluate the peculiarities of metabolic changes in the body of female athletes who specialize in judo, when performing anaerobic loads in different phases of the menstrual cycle Material and methods of research. The achievement of the goal envisaged solving the set tasks using a set of methods, including general scientific, practical and methods of mathematical statistics. The analysis of special scientific and methodical literature included printed literary sources and databases Scopus, SPORT Discus, Web of Science, Scholar. In total, more than 70 sources of information were analyzed, after which a final review was conducted and 30 literature sources were singled out, the material of which was processed using general scientific methods. Biochemical parameters of blood were studied – the content of glucose and lactate in the blood using the «Accutrend Plus» analyzer (Switzerland). The results. It was established that during the test load of anaerobic orientation in different phases of the menstrual cycle, a greater metabolic stress of the body of female athletes was noted both in the postovulatory phase, and at the same time, better results, which indicates a greater metabolic capacity of glycolysis, and in the menstrual and premenstrual phases, which are characterized by the stress of functioning regulatory mechanisms. Conclusions. The obtained results make it possible to purposefully influence the training process of female athletes who specialize in judo, to expand their reserve capabilities, to make adjustments to the training process in order to increase special work capacity.

Post-stress physioprophylaxis test in soccer players: effects of blood lactate concentrations

Objective: To determine the effects of physioprophylaxis (PP) on blood lactate (BL) concentrations after maximal incremental stress test, considering that this is the application of techniques in sports physiotherapy to reduce signs of muscle fatigue that can trigger injuries due to overload. Materials and Methods: Quantitative study, experimental type, longitudinal section in 12 university players. The group is divided into one control group (CG) with recovery at rest without PP and another experimental group (EG) to which PP is applied at the end of the test. Blood lactate is recorded with Accutrend Plus at the beginning of the test, (1) five minutes after finishing the test (2) and after the PP (3) at two different moments for intra-subject analysis. Results: The following data were obtained regarding blood lactate clearance, Moment 1: Without Plan (WoP) 4.86±1.4 and With Plan (WP) 8.85±1.25 (p<0.05), moment 2: (WoP) 5.6±1.76 and (WP) 7.8±1.3 (p<0.05) in mmol/L, and intra-subject: (WoP): 5.25±1.58; (WP): 8.35±1.33 (p<0.05). Conclusions: The clearance of lactate in the blood at 30 minutes post stress test in the EG is bigger than the CG, because they recovered with the physioprophylactic plan.

Applications of Biosensors for Meat Quality Evaluations.

The aim of this study was to apply biosensors in the assessment of meat quality. The research was carried out on 20 samples of the Longissimus muscle obtained from pork of Polish Landrace and Polish Large White hybrids of fattening pigs. In the samples, 48 h after slaughter pH values, color parameters in the CIE system (L* a* b*), the volume of natural drip loss and intramuscular fat content were measured. The commercially available biosensor Accutrend Plus was used to measure glucose, triglycerides and lactic acid in meat juice. Significant (p ≤ 0.05) relationships between glucose, triglycerides, lactic acid levels and pork quality characteristics, i.e., pH (r = −0.62; r = −0.78; r = −0.68 respectively), natural drip loss and (r = 0.57; r = 0.58; r = 0.49), color parameters as L*, a* and b* (r = from 0.47 to 0.79) were demonstrated. The study showed a negative correlation between the intramuscular fat content and acidification of muscle tissue (r = −0.49), and a positive one with the brightness of color (r = 0.46). The results of the canonical analysis show that the measurement of all three metabolites in muscle juice allows the evaluation of the technological quality of meat with an accuracy of 86.54% (Rc = 0.93, p < 0.01).

Capillary Lactate Level in Non-Severe and Severe CommunityAcquired Pneumonia Patients

Background: Community-acquired pneumonia is an inflammatory disease of the lung caused bymicroorganisms acquired from a non-hospital environment. The pneumonia severity index and ATS/IDSAseverity criteria are widely used to predict the severity of CAP. Lactate is a biomarker that can be measuredby point-of-care devices that provide results in a short of time. This study aimed to determine the differencein capillary lactate level between non-severe and severe pneumonia to provide an additional method toquickly stratify pneumonia severity and treat it accordingly.Methods: This cross-sectional study was performed in the emergency room of a regional research hospital.The pneumonia diagnosis was determined by the symptoms, physical examination and radiological findings.Capillary lactate level was measured with Accutrend Plus lactate point-of care device. Severity stratificationwas done according to ATS/IDSA criteria. The mean of lactate level in all subjects was 3.40±1.52, in nonsevere pneumonia subgroup was 2.25±0.94, and 4.56±1.01 in severe pneumonia subgroup. Lactate issignificantly higher in severe pneumonia subgroup. The cut-off point of lactate level for severity group was3.2 mmol/L (95.5% sensitivity, 86.4% specificity).Conclusion: The early prediction of CAP severity is essential to determine the need for admission in theintensive care unit and close follow up. The lactate level can be used for immediate severity stratification inemergency departments.

Evaluation of Blood Glucose and Lactate Concentrations in Mule and Equine Foals

Information about mule physiology is scarce. Glucose and lactate serve as prognostic tools in neonates; thus, real-time evaluations would be beneficial. Our main objective was to measure glucose and lactate concentrations from healthy mule and equine foals from birth to 720 hours. Glucose and lactate concentrations were evaluated with a benchtop Randox Daytona analyzer (LAB) using plasma and with an Accutrend Plus system (ACP) using whole blood. Data were analyzed using PROC MIXED (P < .05), intraclass coefficient correlation and Bland-Altman analysis. Glucose and lactate concentrations from mule and equine foals were different when evaluated with LAB, but there was no difference when evaluated with ACP. Glucose pattern of variation, evaluated with both analyzers for mule and equine foals, had an increase, with subsequent decrease, whereas lactate pattern of variation had initial higher values with subsequent decrease. Intraclass coefficient correlation for glucose was low and moderate for lactate. Results of glucose and lactate with ACP from our experimental neonates were not highly correlated with LAB. However, the ACP had the same pattern of variation for glucose and lactate, thus can still be used clinically for immediate evaluations if the technique is standardized with the specific samples that will be used.

Performance of commercially-available cholesterol self-tests

Background Hypercholesterolemia (plasma cholesterol concentration ≥5.2 mmol/L) is a risk factor for cardiovascular disease and stroke. Many different cholesterol self-tests are readily available at general stores, pharmacies and web shops. However, there is limited information on their analytical and diagnostic performance. Methods We included 62 adult patients who required a lipid panel measurement (cholesterol, high-density lipoprotein (HDL), triglycerides and LDLcalc) for routine care. The performance of five different cholesterol self-tests, three quantitative meters ( Roche Accutrend Plus, Mission 3-in-1 and Qucare) and two semi-quantitative strip tests ( Veroval and Mylan MyTest), was assessed according to the manufacturers’ protocol. Results The average plasma cholesterol concentration was 5.2 ± 1.2 mmol/L. The mean absolute relative difference (MARD) of the five cholesterol self-tests ranged from 6 ± 5% ( Accutrend Plus) to 20 ± 12% ( Mylan Mytest). The Accutrend Plus cholesterol meter showed the best diagnostic performance with a 92% sensitivity and 89% specificity. The Qucare and Mission 3-in-1 are able to measure HDL concentrations and can thus provide a cholesterol:HDL ratio. The Passing-Bablok regression analyses for the ratio showed poor performance in both self-tests ( Mission 3-in-1: y = 1.62x–1.20; Qucare: y = 0.61x + 1.75). The Accutrend Plus is unable to measure the plasma high-density lipoprotein concentration. Conclusions/interpretation: The Accutrend Plus cholesterol meter (Roche) had excellent diagnostic and analytic performance. However, several of the commercially-available self-tests had considerably poor accuracy and diagnostic performance and therefore do not meet the required qualifications, potentially leading to erroneous results. Better regulation, standardization and harmonization of cholesterol self-tests is warranted.

THE EVALUTION OF THE AMOUNT OF TRIGLYCERIDESIN THE BLOOD OF ELDERLY POPULATION (IN THE EXAMPLE OF BAKU)

The purpose of the work. Learning the features of spreading of hypertriglyceridemia among elderly population and its connection with hypercholesterolemia (HXS).Materials and methods. At the same time a survey questionnaire (SF-36 uestionnaire) was conducted and finger-prick blood was tested. The blood test trial was carried out with the help of «Accutrend Plus» portable device. The concentration of triglycerides and cholesterol appeared on the screen within 1-2 minutes with the help of the test system.Results. TG was found in 33,7±1,1% of the population, it was 1,8±2,2 mmol/l in 24,5±1,0%, correspondingly 2,3±5.6 mmol/l (Р&lt;0,005) in 7,7±0,6%, more than 5,7 mmol/l (Р&lt;0,001) in 1,5±0,7%. The random frequency of HTG is rising according to population age increase (q=+0,88±0,09). HTG was 37.0±1,6% among men, and 31,2±1,4% among women (P&lt;0,01). In 25,6±1,0% of cases HTG was observed with HCS, but in 3,1±0,4% of cases it was observed independently.Consequences. Research is important to clarify the role of HTG in the development of cardiovascular diseases in the background of a fairly wide distribution of HTG among the population.

HIGH PREVALENCE OF SUBJECTS WITH HIGH CARDIOVASCULAR RISK IN COMMUNITY PHARMACIES – NEGATIVE TREND IN 2018

Objective: Our aim is to analyze characteristics and global cardiovascular (CV) risk in a sample of subjects who visited pharmacies in Zagreb County on World Hypertension Day 2017 and 2018. This is part of an ongoing collaboration of pharmacist from Zagreb County and Croatian Society of Hypertension. Design and method: Blood pressure (BP) and total serum cholesterol (TC) (Roche Accutrend Plus; not always fasting) were measured by local pharmacists. In 2017, 906 subjects (369 M, 546 F, mean age 53.7, range 21–74) and in 2018, 901 subjects (327 M 574F) who visited pharmacies for various reasons and agreed to participate, were enrolled. BP was measured twice after rest of 5 minutes in a seated position using Omron M6 device and appropriate cuff. Subjects were divided into 2 subgroups depending on smoking habits. There is a risk of sampling bias as only those who agreed to participate were included. Demographic and anthropometric data; cardiovascular risk factors; data on salt intake and opinion about the usefulness of smart-phone applications in hypertension were collected. Results: In 2017 and 2018 BP > 140/90 mmHg was found in 34.7% and 39.7 % (M vs. F; p < 0.0001,), TC > 5 mmol/l in 29.1% and 55.2% (M vs. F; p < 0.0001), and there were 34.9% and 28.4% smokers (M vs. F; p > 0.05), respectively. Heart Score >5% was determined in 20.5% and 17%, >10 % in 6.3% and 6.3%, respectively. Conclusions: In this study, we confirmed our previous findings that subjects with high BP, TC and high global CV risk are regularly visited pharmacies. Unfortunately, there is no positive trend as we failed to find differences between 2017 and 2018. It is of utmost importance to include pharmacists as collaborators in everyday work where they can help with education about healthy lifestyle and adherence, while during public health actions they could identify subjects with high global CV risk who should be transferred to physicians.

PENINGKATAN KADAR ASAM LAKTAT DALAM DARAH SESUDAH BEKERJA

Fatigue is a protective mechanism of the body to avoid further damage resulting in the recovery after the break. It can occur because of a accumulation of lactic acid due to continuous muscle contraction. One of the jobs in Gunawangsa Tidar Superblock Apartment Project at PT. PP (Persero) Tbk. which can lead to fatigue is formwork jobs. The purpose of the study was to analyze the increased concentration of lactic acid in the blood after work on the formwork workers Gunawangsa Tidar Superblock Apartment Project at PT. PP (Persero) Tbk. This study was an observational study with cross-sectional study design. Data obtained by using interviews with questionnaires about sexs, ages, length of work, duration of work, duration of rest, work day and perception of heat exposure. Beside that, data wa collected by measuring lactic acid consentration in the blood with the Accutrend Plus Brand Roche to 30 formwork workers. The independent variable was working while dependent variable was concentration of lactic acid in the blood. Data were tested using Wilcoxon Signed Ranks Test. The results showed that the average concentration of lactic acid in the blood before and after work, namely 0.263 mmol/l and 0.883 mmol/l. Based on statistical test of Wilcoxon Signed Ranks Test, was obtained N Positive Ranks = 16 with Asymp. Sig. (2-tailed) = 0.001. The conclusion of this study that there is an increase in lactic acid consentration in the blood after work.Keywords: blood, fatigue, lactic acid

Maternal Postprandial Triglycerides in the Home Setting

Background and Aims: Elevated maternal triglycerides (TG) have been associated with adverse pregnancy outcomes including an increased risk of preeclampsia and macrosomia. Maternal TG are primarily measured in the fasting state and at single time points during pregnancy. Temporal and prandial changes in TG levels during pregnancy are not well described. The purpose of this study was to examine flux of capillary glucose and TG in late pregnancy, how they are influenced by high and low fat meals, and whether they relate to infant body composition. Materials and Methods: Women in the third trimester of pregnancy completed 4 days of home capillary glucose and TG monitoring (4 times a day (fasting and two hours post each meal)). Using the Roche Accutrend Plus meter. Each woman consumed an isocaloric high fat (41%) and a low fat (25%) breakfast meal on different days during the study period. Area under the curve (AUC) for the capillary glucose and TG measures after the standardized meals was calculated using trapezoidal approximation. AUC for women with and without GDM was compared for each meal and metabolic measure. Infant body fat % was measured by air displacement plethysmography. Results: To date, thirteen women, 7 with GDM and 6 normoglycaemic have completed the study. Median capillary glucose levels: fasting 5.0 (95% CI 4.6-5.2) mmol/L and postprandial 6.0 (95% CI 5.7-6.2) mmol/L. Median capillary TG: fasting 3.3 (95% CI 2.9-3.8) mmol/L and postprandial 3.6 (95% CI 3.2-3.8) mmol/L. Mean infant body fat % 10.6 (SD4.9)%. There were no significant differences in capillary glucose or triglycerides between the meals although capillary glucose trended higher with the low fat meal. GDM status did not affect the AUC glucose or AUC triglycerides for either meal. Conclusion: This study demonstrates that capillary triglycerides can be monitored at home in a similar fashion to capillary glucose. Capillary triglyceride levels were not related to infant anthropometry and did not vary significantly with meal fat content. Recruitment is ongoing. Disclosure H.L. Barrett: None. M. Dekker: Speaker's Bureau; Self; Abbott. Other Relationship; Self; Nestlé. S.J. de Jersey: None. M.C. d'Emden: Advisory Panel; Self; AstraZeneca. Speaker's Bureau; Self; AstraZeneca. Advisory Panel; Self; Eli Lilly and Company. Speaker's Bureau; Self; Eli Lilly and Company, Boehringer Ingelheim GmbH. Advisory Panel; Self; Sanofi. Speaker's Bureau; Self; Sanofi, Novo Nordisk A/S, Novartis Pharmaceuticals Corporation. L. Callaway: None. H. McIntyre: Speaker's Bureau; Self; Novo Nordisk Inc.. Research Support; Self; Danish Diabetes Academy.

Capillary and venous lactate measurements with a handheld device compared to venous blood-gas analysis for emergency patients

Background/aimEarly identification of lactate levels may have a large impact on triage classification and assist in identifying critically ill patients. A handheld device provides a rapid and timesaving measurement of lactate levels adapted to work in a prehospital care setting. I.e., the device is small, fast, and easy-to-use. The aim of this study was to evaluate the Accutrend Plus handheld lactate analyzer in comparison to the reference in-hospital method.MethodsPatients triaged as minimum yellow according to the RETTS System (Rapid Emergency Triage and Treatment System) and transported to hospital by ambulance were selected and a written consent to participate was obtained prior to inclusion in the study. Capillary (CAP) and venous (VEN) blood were analyzed with Accutrend Plus (AP). Venous blood samples were analyzed at the local hospital laboratory (GEM premier 4000) within 20 min from sampling. All sampling was conducted by two registered nurses specially trained in prehospital care.Results480 lactate measurements were performed in 160 patients. The mean difference between measurements in capillary blood compared with the reference method was 0.7 mmol/L and for venous blood 0.9 mmol/L. The limits of agreement from the Bland-Altman plot was − 0.9 to + 2.5 mmol/L and and − 0.1 to + 1.9 mmol/L, for CAP and VEN compared with GEM.ConclusionOur results shows low accuracy and low precision with VEN / CAP measurements of lactate compared to reference GEM.

EFFECT OF RESPIRATORY DEPRESSION ON ORGANISM FUNCTIONALITY IN CYCLING IN TRIATHLON

“Oxygen intake”, “Hypoxia”, “Respiratory muscles training”: are very well known terms in now-a-day sports. That is why the goal of the research is to investigate the effects of the lack of oxygen on an organism’s functionality in the low season preparation term within the cycling stage of triathlon. Nine healthy active triathletes (males n=9), age (20 ± 7 years), competitive at national and international level, were trained in the equal conditions using Spinning bikes in the same room (22 °C ± 2.4 °C and 82 ± 4 % RH), at the same time, at the same cadence (100 revolutions ± 5 rev.) and performing the same exercises. All participants had 4 monocycles pre-experimental preparation (PP). Followed by 12 monocycles (hypoxia training – HT) as a part of a monthly microcycle. During a 60 min session one group was using Ultrabreathe, another group was using the Elevation Mask 2.0 and the last group didn’t use any device performing as a control group. After another 4 monocycles for recovery (R). Data was collected at the end of each phase-PP (4 monocycles in one week), in the middle and at the end of phase-HT (8-monocycles using Elevation Mask 2.0 and Ultrabreathe devices) and the end of phase-R (4-monocycles with no respiration effort). Maximal oxygen consumption (VO2max), Lactate (LA), cadence power (W) and heart rate (HR) was recorded and collected as part of the research. All were measured in laboratory conditions using KORR CardioCoach gas analyzing system for VO2max, cadence power was measured by the Monark LC4R ergometer bike, whereas blood samples were collected for lactate using the COBAS Accutrend Plus device, heart rate data was measured by the POLAR H7 heart rate belts and POLAR Power Flow system. The performances expressed changes in all four parameters on all stages of the experiment. Maximal oxygen consumption showed an increase in two groups who were using the respiratory depression devices after 8 monocycles –by 4,35 and 3,01% respectively, and by the end of the experiment the total difference was - 3,74 and 0,82 % respectively. Improvements were also defined in the level of increase in lactate and maximum cadence power. There was a power increase in the two groups who were using the respiratory depression devices after 8 monocycles - 3,92 and 1,57% respectively, and by the end of experiment the total difference was - 3,57 and 0,87 % respectively. All this data shows us the positive effects of hypoxial training.

Validation of the Accutrend Plus point-of-care triglyceride analyzer in horses, ponies, and donkeys.

To assess the accuracy and reliability of a point-of-care (POC) triglyceride analyzer and to establish reference intervals for blood ([TRIG]POC/WB ) and plasma triglyceride concentrations ([TRIG]POC/PL ) in horses, ponies, and donkeys. Prospective study. University teaching hospital. 120 adult healthy equids (78 horses and ponies, 42 donkeys) and 79 equids suffering from hypertriglyceridemia (73 horses and ponies, 6 donkeys). None. [TRIG]POC/WB and [TRIG]POC/PL were measured using a POC analyzer and plasma triglyceride concentrations were measured using a standard laboratory assay ([TRIG]LAB/PL ). Reference intervals were determined. Test accuracy was assessed by Bland-Altman comparison of POC measurements with standard laboratory measurements and by evaluating linearity of dilutional series. Test reliability was assessed by repeated serial measurements. [TRIG]POC/WB and [TRIG]POC/PL were below the analytic range of the POC assay (<0.8 mmol/L [<70 mg/dL]) in healthy horses and ponies, whereas the reference intervals were 0.82-3.14 mmol/L (73-278 mg/dL) and 0.87-3.02 mmol/L (77-267 mg/dL), respectively, in donkeys. The POC analyzer systematically overestimated triglyceride concentrations when compared to a standard laboratory assay. The difference between [TRIG]POC/WB and [TRIG]POC/PL was small and clinically negligible. The coefficient of variation of repeated measures performed on the POC analyzer was below 10% for [TRIG]POC/WB and [TRIG]POC/PL , both in horses and donkeys and at all concentration ranges. The POC analyzer allows accurate and reliable measurement of [TRIG]POC/WB and [TRIG]POC/PL in horses, ponies, and donkeys in clinical settings. Assay-specific reference intervals should be determined for diagnosis and clinical monitoring of hypertriglyceridemia in equids.

Evaluation of Three Handheld Lactate Meters for Measuring Hemolymph L-Lactate in Decapod Crustaceans

The aim of this study was to determine the reliability and accuracy of 3 commercially available handheld lactate meters when measuring hemolymph L-lactate in decapod crustaceans. The instruments tested were Accutrend Plus (AP), Lactate Scout (LS), and Lactate Plus (LP), and all models were tested in triplicate and compared with a commonly used enzymatic kit for L-lactate quantification (TB-kit). The Norway lobster Nephrops norwegicus and the European lobster Hommarus gammarus were used as model organisms, with emersion as an invoker of hemolymph L-lactate. Between-method comparisons were investigated by determining correlations (Pearson's r) and concordance (concordance correlation coefficient (CCC) and Bland-Altmand's 95% limit of agreement (95% LOA)) between handheld instruments and TB-kit. Within-instrument variation was tested by calculating the coefficient of variation (CV), and the average absolute deviation from the mean (AD). The within-instrument variation was low for all models (CV, 3.8–6.1%; AD, <0.3 mM), showing high reproducibility of technical replicates. A significant correlation with TB-kit was found for all handheld instruments (AP, r = 0.970; LS, r =; 0.956; LP, r = 0.950). For both AP and LS, there was a moderate and significant concordance with TB-kit (AP, CCC = 0.945; LS, CCC = 0.940). The mean difference from TB-kit was -1.42 mM (95% LOA,-4.50–1.66 mM) for AP and 1.19 mM (95% LOA,-3.13–5.51 mM) for LP. Emersion experiments with the European lobster showed that AP and LS were in line with TB-kit in terms of detecting differences in L-lactate levels between groups of animals. As a result of the high frequency of error readings from LP, this instrument was not considered reliable when measuring L-lactate in decapod crustaceans. In conclusion the handheld instruments AP and LS are found to be reliable instruments when measuring hemolymph L-lactate in decapod crustaceans. Comparison of results from different methods should be avoided, and in cases when accurate absolute measurement of L-lactate is needed, methods allowing for greater accuracy and resolution should be used.

Evaluation of 4 Point‐of‐Care Units for the Determination of Blood l‐Lactate Concentration in Cattle

Despite being used commonly in bovine medicine, information on reliability of point-of-care (POC) lactate meters is limited. To determine the validity of 4 commercially available POC lactate meters in cattle. Cattle with various diseases (n = 120). Blood samples collected from the jugular vein were processed immediately on POC lactate meters. Plasma l-lactate concentration was measured by the enzymatic-colorimetric method (ELISA). Data were subjected to Friedman's test for comparison, Passing-Bablok regression and Bland-Altman plot analyses for reliability, and receiver operating characteristics analysis for sensitivity (Se) and specificity (Sp). The POC lactate meters were highly correlated with ELISA (r = 0.98-0.99) despite disagreements among units. When regressed on ELISA, blood l-lactate concentrations generated from Accutrend Plus and Lactate Pro were linear up to 16.6 and 15.7 mmol/L, respectively, whereas those generated from i-STAT and Lactate Scout were linear up to 19.5 and 19.7 mmol/L, respectively. All POC lactate meters had a Se of 100% with Sp of 95.7-98.6% at a plasma l-lactate cut-off concentration of 4 mmol/L. i-STAT had the best accuracy (99.0%) and precision (99.8%), the best linear fit (y = -0.13 + 1.04X) yielding the lowest bias (-6.6%) as well as the highest Se (100%) and Sp (98.6%). Despite high correlation with the reference method, dilution is needed for Accutrend Plus/Lactate Pro and i-STAT/Lactate Scout if concentrations >15 and 20 mmol/L, respectively. i-STAT provided the most accurate and precise results.

Validity of a Portable Glucose, Total Cholesterol, and Triglycerides Multi-Analyzer in Adults

This study investigated the accuracy and precision of the Accutrend Plus system to determine blood glucose, total cholesterol, and plasma triglycerides in adults and evaluated its efficiency in measuring these blood variables. The sample consisted of 53 subjects (≥ 18 years). For blood variable laboratory determination, venous blood samples were collected and processed in a Labmax 240 analyzer. To measure blood variables with the Accutrend Plus system, samples of capillary blood were collected. In the analysis, the following tests were included: Wilcoxon and Student's t-tests for paired samples, Lin's concordance coefficient, Bland-Altman method, receiver operating characteristic curve, McNemar test, and k statistics. The results show that the Accutrend Plus system provided significantly higher values (p ≤ .05) of glucose and triglycerides but not of total cholesterol (p > .05) as compared to the values determined in the laboratory. However, the system showed good reproducibility (Lin's coefficient: glucose = .958, triglycerides = .992, total cholesterol = .940) and high concordance with the laboratory method (Lin's coefficient: glucose = .952, triglycerides = .990, total cholesterol = .944) and high sensitivity (glucose = 80.0%, triglycerides = 90.5%, total cholesterol = 84.4%) and specificity (glucose = 100.0%, triglycerides = 96.9%, total cholesterol = 95.2%) in the discrimination of high values of the three blood variables analyzed. It could be concluded that despite the tendency to overestimate glucose and triglyceride levels, a portable multi-analyzer is a valid alternative for the monitoring of metabolic disorders and cardiovascular risk factors.

Use of the Accutrend Plus point-of-care monitor for blood triglyceride measurement in horses

ELEVATED triglyceride concentrations are a common consequence of negative energy balance in equids (Naylor and others 1980). They are most often observed in overweight ponies, miniature horses and donkeys experiencing...

Reproducibility, accuracy and concordance of Accutrend® Plus for measuring circulating lipid concentration in adults

The determination of lipid biomarkers by capillary sampling may be useful in the screening, diagnosis and/or personal management of hyperlipidemia and cardiovascular risk. It remains unclear whether the use of the Accutrend Plus system is appropriate. This study aimed to assess its reproducibility, accuracy and concordance for blood lipid profiling in adults. Fasting capillary total cholesterol (TC) and triglyceride (TG) concentration on Accutrend Plus were compared with their venous analogues obtained by a laboratory reference method in sixty-one adults (27 men and 34 women, aged 33.0 years). Supplementary capillary sampling was performed at two consecutive days taking into account macro-nutrient intake. The day-to-day reproducibility of the Accutrend Plus system proved to be high for TC (ICC = 0.85, P < 0.001), but moderate for TG (ICC = 0.68, P < 0.001). Strong correlations (r > or = 0.80, P < 0.001) with the reference method were found for TC and TG. Mean difference (limits of agreement) were: 0.26 mmol/L (-0.95, 1.47) for TC, and -0.16 mmol/L (-1.29, 0.98) for TG. The concordance for subject classification according to the National Cholesterol Education Program (NCEP) guidelines was significant (P < 0.001), with substantial agreement for TC (K(w) = 0.67), and moderate agreement for TG (K(w) = 0.50). Day-to-day reproducibility of the Accutrend Plus device for TC and TG is not optimal and lacks accuracy when compared to the reference laboratory method. The concordance between both methods for classifying subjects according to the NCEP is inadequate. Accutrend Plus device should not be interchangeably used as a substitution for the standard laboratory methods in the diagnosis of hyperlipidemia.

Effects Of Exercise Prescription Program Among Sedentary, Overweigh Or Obese Adults With Hypertension Or Diabetes.

PURPOSE: To explore the effects of an 8-week exercise prescription program based on high intensity (9 METs or greater) cardio-respiratory fitness and resistance training among sedentary, overweight or obese adults with hypertension or diabetes METHODS: seventy adults (28 women, 42men) age 47-64 recieved the prescribed program (8 week high intensity cardiovascular and resistance training two times per week) other 60 adults recieved another program (couselling): cholesterol, triglycerides and blood glucose were measured at the begining and at the end of the program and one month after the end of the program with the Accutrend Plus (roche), also physical fitness was measured with the new ALPHA fitness battery. Blood presure was measured with omron MIT elite plus. RESULTS: experimental group has a lot increased health levels respect to control group and also comparing the experimental group at the begining of the program. physical fitness has increased 16% in cardiovascular fitness, 50% in strength and almost 120% in flexibility. CONCLUSIONS: 8 week prescription program based on high intensity cardio vascular and resistance training present very high benefits in terms of cholesterol, triglycerides and blood glucose and also in physical fitness in overweight adults.