Soil Organic Matter Accumulation Research Articles

Molecular Weight Distribution of Humic Acids Isolated from Calcic Cryosol in Central Yakutia, Russia.

The transition of soils into fallow state has a significant impact on the accumulation and transformation of soil organic matter (SOM). However, the issue of SOM transformation as a result of soil transition to fallow state in cryolithozone conditions is insufficiently studied. The aim of this study is to investigate the molecular weight (MW) distribution of humic acids (HAs) isolated from soils of central Yakutia. Native, fallow and agricultural soils in the vicinity of Yakutsk city were studied. MW distributions of HA preparations were obtained on an AKTAbasic 10 UPS chromatographic system (Amersam Biosciences, Uppsala, Sweden) using a SuperdexTM 200 10/300 GL column (with cross-linked dextran gel, fractionation range for globular proteins 10-600 kDa). The data on the molecular-mass distribution of HAs of fallow and agricultural soils of Central Yakutia were obtained for the first time. According to the obtained data, it was found that the highest carbon content in the structure of HAs was observed in agricultural soils (52.56%), and is associated with soil cultivation and fertilizer application. Among the HAs of fallow soils, we note that those soils that are in the process of self-vegetation have a relatively high carbon content in the HAs (45.84%), but the highest content was observed in fallow soils used as hayfields (49.98%), indicating that the reinvolvement of agriculture in fallow soils leads to an increase in the carbon content of HAs. According to the data of the MW distribution of HAs, it was found that the highest content of a high MW fraction of HAs was recorded in native soil (18.8%); this is due to the early stages of humification and the low maturity of organic matter. The highest content of a low MW fraction of HAs was recorded in agricultural soil (73.3%); this is due to the formation of molecular complexes of a "secondary" nature, which are more stable in the environment than the primary transformation products of humification precursors. The molecular composition of the HAs of fallow soils in the process of self-overgrowing is characterized by values closer to the HAs of native soils, which indicates their transformation towards HAs of native soils. The obtained results indicate that the reinvolvement of fallow soils leads to the transformation of the molecular composition of HAs towards HAs of agricultural soils, and to an increase in the resistance of SOM to biodegradation.

Balanceando el nitrógeno en la portera del predio

Uruguay's dairy can potentially enhance milk productivity competitively, but intensification risks elevating nitrogen (N) surplus, heightening environmental concerns. This study quantified farm-gate N inputs and outputs, calculating N surplus (input-output) and N use efficiency (NUE=output/input) for 17 commercial modal dairy systems identified in the 2014 and 2019 national surveys and 6 prospective intensified systems based on experimental pastoral farmlets achieving near-maximal rainfed productivity. Current dairy systems maintained N surplus at 71 kg N ha-1 between 2014 and 2019 (range: 44-97 kg N ha-1) while improving NUE from 28.3 to 30.5% (range: 20-35%). Intensification increased N surplus without necessarily reducing NUE. Our analyses highlight three aspects: (i) comparatively low N surplus of current Uruguayan dairy, (ii) nonlinear links between N surplus and stocking rate, feed intake, milk productivity and operating profit, and (iii) inequality between dairy systems in their contribution to national dairy N surplus reflects mainly disparity in farm size. These insights underscore the crucial need for understanding the actual fate of N surpluses: nitrate leaching, ammonia volatilisation, N2 denitrification, or N accumulation in soil organic matter. This is an unavoidable requisite for designing management practices and policies able to effectively optimise the economic and environmental sustainability of Uruguayan dairy.

Soil organic carbon and nutrient characteristics of Anogeissus groves in old Opara forest reserve, Nigeria.

Forest-savanna transition zones of West Africa are uniquely characterized by forest groves, forest patches, or forest islands, the importance of which for sustainable resource management and their potential for carbon sequestration and nutrient cycling is often underrated. Our study conducted a comparative analysis of the soil organic carbon and nutrient characteristics of the Anogeissus groves in the old Opara forest reserve and their adjoining arable lands. We established 30 sampling frames of 100m × 100m plots with 15 frames per land use type. For each sampling frame, six observation points were randomly selected, and composite soil samples were collected at soil depths of 0-20cm and 20-50cm per observation point. Our results showed Anogeissus groves and their adjoining arable lands to exist on similar landscapes while the groves have enriched soil morphological characteristics (e.g., soil color), higher soil organic carbon (SOC), and better nutrient characteristics. There were strong positive relationships between SOC, effective cation exchange capacity, total nitrogen, calcium, magnesium and calcium, zinc, electrical conductivity, and copper. The significant soil organic matter accumulation in the groves accounts for the overall improved soil characteristics over the adjoining arable lands. Preserving the groves and similar Africanecosystems may be important in climate regulation, resources and biodiversity conservation, and ethnopharmacology for rural communities. Thus, a question arises: should more land be set aside for ecological conservation or for agricultural productivity?

Nutrient supplementation changes chemical composition of soil organic matter density fractions in desert steppe soil in northern China

The influence of reactive nitrogen (N) and phosphorus (P) supplementation on ecosystem dynamics of soil organic matter (SOM) shows extensive variations. The underlying mechanisms of regulation of the chemical composition and dynamics of different SOM density fractions (DFs) in aggregates in a desert steppe by N and P supplementation are unclear. Here, the chemical composition and organic carbon (C) and N dynamics of various SOM DFs under N and P addition were assessed. Surface soil samples taken at 0–10 cm depth were obtained from 4-year (2017–2021) experiments under four conditions, namely N treatment, P treatment, combined N and P treatment (NP), and no nutrient supplementation (CK), in a desert steppe located in northern China. Nutrient supplementation significantly increased (P < 0.05) SOM content by 8.89–35.4%; however, the total N content remained constant. N addition mainly increased SOM content in the macroaggregates (>0.25 mm), while P enhanced SOM in microaggregates (0.25–0.053 mm) and silt-clay (<0.053 mm). The fine particulate organic matter (fPOM) proportion was increased significantly (P < 0.05) by 18.4% and 16.4% in macro- and microaggregates under N addition, respectively; P addition mainly enhanced mineral-associated SOM (mSOM) proportion in aggregates. N supplementation remarkably enhanced (P < 0.05) fPOM’s organic C content by 27.3% and 32.0%, and N content by 40.9% and 145.7% in macro- and microaggregates, respectively. Single N and P addition promoted the enrichment of labile and resistant organic components in light fraction (LF), respectively. N promoted the enrichment of stable organic components in the mSOM of microaggregates. Nutrient inputs drive mSOM formation through the interaction of carboxyl-C and mineral surface. Based on a stronger δ13C and δ15N signal, N addition enhanced SOM decomposition in aggregates. Nutrient supplementation promoted SOM accumulation through the formation of more stable old C in POM and silt-clay. Our findings suggest that SOM accumulation in desert steppe probably increases following nutrient addition, but the mechanism is different through which N or P enrichment affects soil dynamics and chemical composition of SOM DFs in desert steppe.

Interaction between ectomycorrhizal and ericoid mycorrhizal plants decelerates stable soil organic matter decomposition

Boreal forests are N-limited ecosystems storing globally significant amounts of carbon (C) belowground as soil organic matter (SOM). The significant role of mycorrhizal fungi, both ericoid (ERM) and ectomycorrhizal (ECM) in decomposition and building up SOM has been proposed, but it is still largely unknown how these two types of mycorrhiza interact in terms of SOM changes. Here we try to elucidate the combined effect of these mycorrhizal types on the SOM decomposition and accumulation in a pot study with ECM-pine (Pinus sylvestris L.) and ERM-heather (Calluna vulgaris L.). Experimental design included plants growing alone or in a mixture, and a non-planted control. In contrast to the mixture, heather growing alone exerted higher biomass, higher uptake of N from stable SOM pool, slightly higher microbial biomass C and fungal biomass. Moreover, heather soil had the highest dissolved organic carbon and nitrogen. On the contrary, mixed cultures showed lower growth of heather and stable SOM decomposition. Our results suggest that interaction between ERM and ECM plants may lead to stable SOM accumulation in boreal forest soils.

No-tillage farming for two decades increases plant- and microbial-biomolecules in the topsoil rather than soil profile in temperate agroecosystem

The impact of conservation tillage on the composition, stability, and origin (plant- or microbial-derived) of soil organic matter (SOM) within soil profiles remains unclear. In this study, we characterized the impact of different tillage practices on the molecular composition and status of SOM. These tillage practices included conventional tillage (CT) and conservation tillage such as rotary tillage (RT) and no-tillage (NT). Our investigation spanned a two-decade period within a temperate agroecosystem. Soil samples were collected down to a 30 cm profile, and four biomarkers, namely free lipids, lignin phenols, neutral sugars, and amino sugars, in conjunction with 13C NMR techniques were used to quantify SOM characteristics. The results revealed that conservation tillage (especially NT) led to improvements in both the quantity and composition of SOM when compared to CT in the topsoil (0–5 cm), but not in deeper layers (5–30 cm). More precisely, the NT topsoil, as opposed to CT, exhibited enhancements in two categories: (1) an increase in plant-derived compounds such as long-chain lipids (≥ C20) and steroids by 51%, lignin phenols by 106%, and plant-derived neutral sugars by 61%, and (2) an augmentation in microbial-derived biomolecules, which includes microbial-derived neutral sugars by 49% and microbial necromass carbon (MNC) by 94%. Furthermore, NT, relative to CT, increased the contribution of MNC to soil organic carbon in topsoil (up to 64%, mainly fungal necromass). This highlighted the predominant role of microbial-derived biomolecules in SOM formation. Similarly, RT treatments enhanced the microbial-derived neutral sugars by 18%, plant-derived neutral sugars by 14%, and lignin phenols by 43% relative to CT in the topsoil. Collectively, our study suggests that NT practice could be considered an effective strategy for enhancing SOM accumulation and persistence by fostering the accrual of plant- and microbial-derived biomolecules in the topsoil.

Effects of long-term no-tillage and different stover mulching amounts on soil carbon and nitrogen contents and enzyme activities of carbon and nitrogen cycle in Mollisols.

To understand the effects of different stover mulching amounts in no-tillage on soil carbon and nitrogen contents and enzyme activities, finding a stover mulching amount which can meet the requirement of soil carbon and nitrogen accumulation while maximizing economic benefits, we conducted a long-term conservation tillage field experiment since 2007 in Mollisols area of Northeast China. We analyzed soil carbon and nitrogen contents, enzyme activities and economic benefits under conventional tillage (Control, CT), no-tillage without stover mulching (NT0), no-tillage with 33% stover mulching (NT33), no-tillage with 67% stover mulching (NT67), and no-tillage with 100% stover mulching (NT100) before planting in May 2020. The results showed that compared with CT, NT0 did not affect soil organic carbon (SOC) and total nitrogen (TN) contents, but increased soil organic carbon recalcitrance and decreased the availability of dissolved organic nitrogen (DON) and ammonium nitrogen. Compared with NT0, no-tillage with stover mulching significantly increased SOC contents in 0-10 cm layer and increased with the amounts of stover. In addition, NT67 and NT100 significantly increased SOC stocks, facilitating the accumulation of soil organic matter. The effects of different stover mulching amounts on soil nitrogen content in 0-10 cm layer were different. Specifically, NT33 increased DON content and DON/TN, NT67 increased DON content, while NT100 increased TN content. Compared with CT, NT0 decreased peroxidase (POD) activity in 0-10 cm layer. Compared with NT0, NT33 increased β-glucosidase (βG), cellobiase (CB), 1,4-β-N-acetylglucosaminidase (NAG), polyphenol oxidase (PPO) and POD activities, while NT67 only increased CB, NAG and POD activities in 0-10 cm soil layer, both alleviated microbial nutrient limitation. NT100 increased PPO activity in 10-20 cm layer. NT33 increased carbon conversion efficiency of stover compared with NT100, and had the highest economic benefit. In all, no-tillage with 33% stover mulching was the optimal strategy, which could promote nutrient circulation, boost stover utilization efficiency, improve the quality of Mollisols, and maximize guaranteed income.

Study in a long-term laboratory experiment of the potential susceptibility to mineralization of organic matter in post-agrogenic light gray soils

Relevance. Since 2021, work has begun in Russia to assess the fertility of unused arable land and its involvement in agricultural circulation. Changes in the farming system of post-agrogenic soils can lead to uncontrolled mineralization of newly formed soil organic matter (SOM) under fallow vegetation and significant CO2 emissions into the atmosphere. Studies of the nature of SOM accumulation under fallow vegetation and assessment of its potential susceptibility to mineralization are relevant, since they can become the basis for the development of agrotechnical methods for returning unused lands to arable circulation with the maximum preservation of their fertility.Methods. In a long-term laboratory incubation experiment, we studied the dynamics of changes in the intensity of basal (BR) and substrate-induced respiration (SIR) in postagrogenic soils to assess the potential susceptibility to SOM mineralization with a change in land use. Layered samples (0–10 and 10–20 cm) were used from the old arable horizons of two fallow plots with different humus conditions. The results of the incubation experiment were compared with the results of assessing the quantitative content and qualitative composition of SOM.Results. In the 0–10 cm layer, the respiration rates are higher than in the 10–20 cm layer. The results of the assessment of the respiration intensity are consistent with the assessment of the qualitative composition of SOM. The accumulation of SOM occurs mainly in the upper part of postagrogenic soils due to mobile easily oxidized organic compounds of a fulvic nature. When developing agrotechnical methods for returning fallow lands to arable circulation, it is necessary to focus primarily on basic processing technologies that ensure maximum preservation of potentially easily mineralized material accumulated in the upper layer.

Carbon reserves on the soils of the Dmitrievsky village council of the Ufa district of the Republic of Bashkortostan

The research was carried out within the framework of the state task of the Ministry of Education and Science of the Russian Federation “Program for the creation and operation of a carbon landfill in the Republic of Bashkortostan “Eurasian carbon landfill” for 2022-2023 (Publication number: FEUR-2022-0001). Carbon deposition in the soil is an essential function arising from the interaction of ecological processes. Human activity influencing these processes leads to dehumidification or, conversely, deposition of soil carbon. The article presents the materials of the analysis of literary sources on the subject of carbon farming, as well as the content of organic matter in the soils of agricultural lands of the southern forest-steppe of the Republic of Bashkortostan in the conditions of a carbon landfill in the Ufa district (Russian Federation). This study aims to analyse carbon reserves in the humus-accumulative soil horizon of a carbon landfill on agricultural lands in the Dmitrievsky Village Council of the Ufa district of the Republic of Bashkortostan. An agrochemical analysis of the accumulation of soil organic matter was carried out, and the capacity of the humus horizon of the soils of this site was determined. Carbon reserves in the studied soils were determined according to the methodology for quantifying greenhouse gas emissions and uptake [Order of the Ministry of Natural Resources of Russia No. 371 dated 05/27/2022, Guidelines for Quantifying the volume of greenhouse gas uptake]. The carbon content in the organic matter of soils is assumed to equal 58%. The conversion to the stock of soil carbon was carried out, considering the density of the soil composition. The degree of saturation of the soils of the studied sites, including the carbon landfill, is calculated. The reserves of organic carbon under individual varieties of soils in the humus-accumulative horizon - 4616360 tons were estimated.

Three years of cover crops management increased soil organic matter and labile carbon pools in a subtropical vegetable agroecosystem

AbstractCover crops have been widely adopted to improve soil functions in agroecosystems, including providing carbon (C) inputs that can contribute to soil C sequestration. However, soil C changes may be slow after introducing cover crops in unfavorable environments for soil organic matter (SOM) accumulation, like the Southeast United States subtropical region characterized by a warm humid climate, and coarse‐textured soils. We examined labile C pools as potential early indicators of SOM changes after cover crop introduction in a sandy subtropical vegetable production system. We compared the effects of four cover crop monocultures namely two grasses [sorghum sudangrass, Sorghum bicolor × S bicolor var. Sudanese and pearl millet, Pennisetum glaucum (L.) R. Br.], two legumes (sunn hemp, Crotalaria juncea L., and cowpea, Vigna unguiculata Walp.), and one four‐species mixture on soil organic carbon pools for 3 years. Soil samples were collected at a 15‐cm depth before cover crop planting and post cover crop incorporation to assess changes in SOM, permanganate‐oxidizable carbon (POX‐C), mineralizable carbon (Cmin), and water extractable organic carbon (WEOC). The incorporation of cover crops increased concentrations of SOM, POX‐C, and Cmin in year 3 relative to their baseline values in year 1. Concentration of SOM increased by 0.24 ± 0.05% (mean ± standard error) after 3 years of cover crop management. However, concentrations of WEOC significantly decreased in years 2 and 3 relative to the baseline. Monocultures and the mixture had similar effects on measured C pools, likely due to comparable aboveground biomass production. Our findings highlight the potential of POX‐C and Cmin as early indicators of SOM accumulation driven by cover crops use, as well as the capacity of cover crops to build SOM in similar subtropical systems and coarser textured soils.

Long‐term application of different organic and inorganic fertilizers in no‐tillage crops changes the soil microstructural viscoelasticity and shear resistance to transient stresses

AbstractThe processes involved in deformation, internal strength and stability of soils with long‐term application of fertilizers (organic and inorganic sources) remain poorly investigated and hence understood, particularly in agricultural systems under subtropical climatic conditions. We investigated how long‐term fertilizer management with organic and inorganic amendments in no‐till crops affects the microstructural stability of a sandy Alfisol under oscillatory shear. The study was conducted in southern Brazil on a 17‐year completely randomized block experiment with five fertilizer treatments: pig slurry (PS), cattle slurry (CS), pig deep litter (PDL), mineral fertilizer (MF) and control, i.e. unfertilized (CL). Soil samples were collected from two layers (0–5 and 5–15 cm) for physical and chemical analyses and evaluation of soil rheological properties under oscillatory shear at two matric potentials (0 and −10 kPa). Organic matter accumulation in soil provided by the PDL and CS fertilizers resulted in higher soil stability and elasticity under oscillatory shear, especially in the 0–5 cm layer. Conversely, MF and PS enhanced the soil susceptibility towards deformation under transient stresses, mainly in the 0–5 cm layer under saturated conditions. The PDL significantly increased soil shear resistance under low‐shear strain conditions. Significant differences ceased under high‐shear strain conditions, though PS and MF yielded at significantly lower strains. Hence, under subtropical conditions, long‐term application of organic fertilizers with fibrous components promoted soil microstructure strengthening, reducing soil susceptibility to erosive processes and compaction.

Assessing the Impact of Erosion and Farming Practices on the Spatial Distribution of Topsoil Characteristics in a Sloping Vineyard Using an Open-source QGIS Software

Erosion is one of the major threats that negatively affect agricultural soils. Several studies indicated that sloping vineyards are prone to soil erosion due to the soil management method. This study was conducted in a sloping vineyard planted in 2019 (with a slope of 100 and a slope length of 38 m) in Thu Cuc commune, Tan Son district, Phu Tho province, Vietnam. The main objective of the study is to evaluate soil characteristics (pH, particle-size distribution, organic matter, cation exchange capacity (CEC), N, P, and K macronutrients) related to terrain morphology, soil erosion, and farming practices. The open-source QGIS software was used to evaluate the spatial distribution of the soil characteristics. We found that the organic matter content, CEC, and clay content in the soils taken at the footslope zones were higher than those at the top of the hill as a result of soil erosion. In contrast, higher contents of K and silt were observed at the top of the hill compared to the footslope, indicating an unapparent impact of soil erosion on these two factors in the studied vineyard. Phosphorus was evenly distributed throughout the plot and influenced by the soil characteristics. Indeed, a strong correlation between total P content and soil pH, organic matter, and silt content was revealed in our study. On the other hand, cultivation practices exerted a significant impact on the prevailing accumulation of soil organic matter, CEC, total N, and clay particle size at the lower part of the vine rows following the horizontal flow. The vine rows were planted along the contour lines rather than the dominant slope of the vineyard can explain this tendency. In addition, no cover crops sown between the vine rows probably resulted in intense soil erosion at the steepest part of the terrain. Overall, the terrain morphology and farming practices played an essential role in the spatial distribution of soil characteristics. Therefore, integrating geographic information systems into building a digital database and predicting the spatial distribution of soil parameters is necessary to perform soil quality monitoring and serve sustainable land management.&#x0D;

Stabilization of organic matter in soils: drivers, mechanisms, and analytical tools – a literature review

ABSTRACT Soils are the largest terrestrial carbon (C) reservoir, and most of this C is retained as soil organic matter (SOM). Due to its ability to capture, stabilize, and store C for extended periods, soils are considered important allies in decarbonizing the atmosphere. The term ‘C stabilization’ includes a series of mechanisms or processes by which soil C is protected within soils and its losses are reduced through microbial decomposition or leaching. Due to their relevance in the global C cycle, C stabilization mechanisms have received intensive attention from the scientific community. As new analytic technologies push the boundaries of what was previously possible to know, new paradigms emerge. This literature review summarizes the current knowledge of the main mechanisms that may promote SOM stabilization. Factors that govern accumulation of SOM are also addressed. We highlight the role of organo-mineral associations and spatial inaccessibility of SOM due to occlusion within soil aggregates to understand the relative contribution of these mechanisms in different soil conditions (e.g., soil texture, mineralogy, and land- use). In addition, the contribution of cutting-edge approaches and analytical techniques to advance the understanding of SOM protection is presented. Modern techniques to evaluate SOM on a micro, nano, and molecular scale can contribute to the mechanistic understanding of SOM stabilization and the study and adoption of management strategies that maintain and increase C stocks in soils.

Effect of organic amendments on soil structure, microbial community and water transport in the Qinghai Lake watershed, North-Eastern Qinghai–Tibet Plateau

ABSTRACT The effect of organic amendments on soil structure, microbial community and water transport in alpine ecosystems remains uncertain. Six fertilization regimes from a 2-year field experiment were selected to study this mechanism, i.e. plant litter (PL), organic fertilizer (OF), low concentration of glucose (LCG), medium concentration of glucose (MCG), high concentration of glucose (HCG) and the control (CT). Our results indicated that the soil macroporosity, branch density, length density and node density of soil macropores treated with OF, PL, HCG, MCG and LCG were larger than those of soils treated with CT. The phospholipid fatty acids (PLFAs), bacteria, fungi, actinomycetes, gram-positive bacteria (G+) and gram-negative bacteria (G−) of soils treated with OF, PL, HCG and MCG were larger than those of soils treated with LCG and CT. In contrast, the dyed area ratio and the maximum wetting depth of soils treated with OF, PL, LCG, HCG and MCG were smaller than those treated with CT. There were significant positive correlations between soil organic matter (SOM) and connectivity of soil macropores. Therefore, SOM accumulation significantly improved the soil pore structure, induced the development of the soil microbial community, increased the soil water retention ability and decreased the generation of preferential flow.

Impact of the sustainable agricultural practices for governing soil health from the perspective of a rising agri-based circular bioeconomy

Soil is a precious and nonrenewable source for agroecosystems of which own health state over time is becoming a focus of global concern. A healthy soil is a harmonious and very complex social system with a good structure, an optimal functional state, and an efficient buffering capacity to maintain a dynamic balance among all productivity factors. There is an urgent need to develop new approaches for the sustainable management of soil quality in depleted soils from a long-term perspective for increasing agricultural productivity and maintaining food security due to global population expansion. Since high crop yield mainly depends on the improvement of soil quality, further efforts must be addressed to develop advanced technologies/processes in reusing waste biomass accordingly with the circular bioeconomy principles. Although the knowledge of relationships between intrinsic factors of soil fertility and crop productivity is the baseline for the optimal soil management, this issue is nevertheless overlooked by stakeholders. Soil organic matter (SOM) associates carbon availability with the plant nutrients (mainly nitrogen, phosphorus, and potassium) leading to the strongest positive impacts of the environmental functions and food production. Unfortunately, there is a progressive trend to lose the SOM stock, so altering the biological functions of the soils. Despite, farmer overlooks the mechanisms for preserving SOM accumulation by adopting inappropriate practices to counteract soil decline consequently to climate change and deterioration of agroecosystems. A wide spread of microplastic and nanoplastic pollutants (MNPs) in the environment have added further concerns due to their potentially hazardous risks for soil health thanks to their ubiquitousness and persistence. Nonetheless, the interactions of MNPs with the soil components, microbial communities, plants, and fauna that could determine the strongest impacts on the nutrient availability and food security are still poorly explored. This review work launches some challenges to reader by providing practical solutions, viewpoints, future challenges, and new perspective for restoring soil health by contrasting soil decline from a long-term perspective in organic farming systems in a sustainable agri-based circular bioeconomy system.

Utilization of bagasse as a soil amendment in sugarcane production on mineral soils in Florida

A 4-year study tested the feasibility of using bagasse as a soil amendment in sugarcane production on a commercial field with mineral soil in Florida. The experiment was established as a completely randomized design trial with three rates of fresh bagasse applications: approximately 5 cm of bagasse, 10 cm of bagasse, and 10 cm of bagasse + nitrogen (N), which are equivalent to 85 t ha–1, 170 t ha–1, and 170 t ha–1 + 336 kg ha–1 ammonium nitrate, respectively. A control (no bagasse and no N added) was also included. As each treatment and the control had three replicates, there were 12 plots (6.5 ha each in size) totaling over 77 ha. The high pH of the soils is a result of years of mixing underlying limestone (calcium carbonate) bedrock with the topsoil. Overall results indicate that bagasse application positively affected the accumulation of soil organic matter during a short-term period, resulting in higher water-holding capacity and lower bulk density. The high rate of bagasse application had a positive effect on N accumulation, extractable phosphorus (P) and potassium (K) contents during the short term. One single application of bagasse significantly increased the first-year sugarcane biomass and sugar yield by approximately 23%. An overall higher application rate of bagasse (10 cm) was recommended as it showed significantly positive effects on soil health indicators and had a longer effect on increasing sugarcane biomass and sugar yield. There is potential to incorporate bagasse into commercial sugarcane production grown on mineral soils in Florida.

Unexpected high alkyl carbon contents in organic matter-rich sandy agricultural soils of Northwest Central Europe

Carbon sequestration in Plaggic Anthrosols is most often investigated by bulk soil carbon inventories, without considering the form in which the carbon is stored (e.g., particulate or mineral-associated organic matter (OM), its capacity, or its chemical composition). Here, we focus on the unusual high organic carbon (OC) accumulation in sandy Plaggic Anthrosols and adjacent reference soils under agricultural use. In these soils, the mineral fraction ≤20 µm which is commonly assumed to be the major factor for OC stabilization, are very low in mass proportion. Soil organic matter (SOM) physical fractionation was done to evaluate the quantity and quality of OC in the topsoil (Ap horizon). For the fraction ≤20 µm (medium and fine silt-, and clay-sized particles), we measured the concentration of OC and calculated its OC storage capacity and contribution. The OC of the fraction ≤20 µm was radiocarbon-dated and analyzed for its chemical composition by solid-state 13C NMR spectroscopy. The highly sandy (∼90 % sand and coarse silt) soils showed an accumulation of OC much higher than the conventionally calculated saturation level controlled by the proportion of the fraction ≤20 µm. Unexpectedly, Plaggic Anthrosols and the respective reference soils showed similar fractional OC concentrations, radiocarbon ages, and OM composition. The isolated fraction ≤20 µm contained, on average, 81 % of the total soil OC in only 9 % of the corresponding soil mass. All soil fractions ≤20 µm are characterized by a high mean OC concentration in the topsoil (reference soils: 226 ± 66.5 mg OC g−1, Plaggic Anthrosols: 202 ± 59.0 mg OC g−1) with a C/N ratio of 15 on average for both soils. The OM composition of the fraction ≤20 µm was specifically rich in alkyl-C, with unusually low proportions of O-alkyl-C and low contents of aryl-C. The radiocarbon concentration (F14C) indicated that topsoil OM of the ≤20 µm fraction is stored for long time periods with high mean conventional radiocarbon ages (14C) not only for Plaggic Anthrosols (F14C: 0.92 ± 0.04; 14C: 639 yBP) but also for the reference soils (F14C: 0.93 ± 0.04; 14C: 575 yBP) and received low inputs of OC derived from recent photosynthesis. Our data indicate the existence of specific SOM accumulation processes in the investigated sandy agricultural soils, resulting particularly large SOM stocks which cannot be explained by mechanistic association of OM with mineral surfaces. It is not clear, if this inherited OM is stable under present-day soil and management conditions.

Short-Term PE Generation Processes in the Soils of a Farmer Plots Network in the Madagascar Highlands: Actors and Drivers

Carbon sequestration in agricultural soils, through the accumulation of high-quality organic matter, represents great potential to mitigate climate change and simultaneously improve soil fertility. Such a challenge is particularly important and relevant in developing tropical countries like Madagascar, where soil carbon storage is vulnerable to climatic variations and where fertilization is generally applied through amendments in organic matter of various origins. The priming effect (PE) is considered here as the stimulation of the mineralization of soil organic matter (SOM) by a supply of fresh organic matter (FOM). PE results from different microbial processes driven by specific biotic and abiotic parameters. Depending on the processes involved, it has been suggested that PE could either counteract SOM accumulation or promote it. The objective of the present study was to explore the relationships between certain agricultural practices (type of crop, quality of fertilization, association with trees), the potential intensity of PE, as well as several abiotic (texture, quantity and quality SOM, nutrient enrichment) a1nd biotic (biomass and phylogenetic composition of microbial communities) factors which have been proposed in the literature as specific determinants of the different PE generation mechanisms. The soils for this study come from a network of farms in a commune in the Highlands of Madagascar. The PE, generated by a supply of 13C-enriched wheat straw, could not directly correlate with agricultural treatments. However, several indirect correlations could be found via several specific abiotic and microbial determinants that are discussed in terms of soil fertility restoration.

Soil color mapping based on Munsell system in the northeast of China

Soil color is the most readily available soil property, soil scientists often use the Munsell system to describe soil color and to quickly understand the physical, chemical, and biological properties of soil through soil color variation. Currently, soil color mapping studies often rely on indirect acquisition of Munsell color data from soil spectral information. As a result, there may be some discrepancies between the mapped colors and the colors perceived by humans. In this study, we collected 880 dry and 827 moist Munsell samples from the Northeast China to test the method developed for soil color mapping. We selected a set of covariates (climate, topography, evapotranspiration, regolith thickness, and remote sensing images) for digital soil color mapping, these covariates were related to soil formation or organic matter accumulation processes. Finally, the dry and moist topsoil color mapping with 30-m resolution was obtained for cropland in Northeast China. These covariates all played positive roles in the soil classification process, among which annual temperature mean (MAT), mean annual precipitation (MAP), land surface temperature (LST), and mean annual potential evapotranspiration (MAPET) had the highest contribution. We summarized the difficulties that usually occur in soil color mapping, and put forward a ‘stepwise classification’ method. According to the three-dimensional attributes of the Munsell system, the measured color data were divided into seven color groups (four dry and three moist color groups). Among all color groups, the highest accuracy of mapping validation was 0.69 and the lowest was 0.58. The kappa coefficient, usually affected by classification bias, was small (0.3). The color differences between measured and predicted colors were found to be 1.21 (dry color) and 1.36 (moist color). Compared to direct classification, the stepwise classification method improved classification accuracy and reduced mapping color differences. Our soil color mapping for two cases of dry and moist corresponded well with spectral color maps. The correlation between soil organic matter (SOM) content and soil color (dry and moist) was analyzed in 807 samples. The darker the soil color was, the higher the SOM content was. In summary, soil color holds the potential to serve as a surrogate for assessing cropland quality, particularly in evaluating SOM.

Drivers of microbially and plant-derived carbon in topsoil and subsoil.

Plant- and microbially derived carbon (C) are the two major sources of soil organic matter (SOM), and their ratio impacts SOM composition, accumulation, stability, and turnover. The contributions of and the key factors defining the plant and microbial C in SOM along the soil profile are not well known. By leveraging nuclear magnetic resonance spectroscopy and biomarker analysis, we analyzed the plant and microbial C in three soil types using regional-scale sampling and combined these results with a meta-analysis. Topsoil (0-40 cm) was rich in carbohydrates and lignin (38%-50%), whereas subsoil (40-100 cm) contained more proteins and lipids (26%-60%). The proportion of plant C increases, while microbial C decreases with SOM content. The decrease rate of the ratio of the microbially derived C to plant-derived C (CM:P ) with SOM content was 23%-30% faster in the topsoil than in the subsoil in the regional study and meta-analysis. The topsoil had high potential to stabilize plant-derived C through intensive microbial transformations and microbial necromass formation. Plant C input and mean annual soil temperature were the main factors defining CM:P in topsoil, whereas the fungi-to-bacteria ratio and clay content were the main factors influencing subsoil CM:P . Combining a regional study and meta-analysis, we highlighted the contribution of plant litter to microbial necromass to organic matter up to 1-m soil depth and elucidated the main factors regulating their long-term preservation.