Accumulation Of Secondary Metabolites In Plants Research Articles

NtWIN1 regulates the biosynthesis of scopoletin and chlorogenic acid by targeting NtF6′H1 and NtCCoAMT genes in Nicotiana tabacum

Scopoletin and chlorogenic acid (CGA) are important polyphenols that regulate plant growth, development, and stress resistance. The ERF transcription factor WAX INDUCER1 (WIN1) promotes the biosynthesis of cutin, suberine, and wax. However, its full roles in regulating the accumulation of plant secondary metabolites still remain to be further clarified. In this study, NtWIN1 gene encoding a SHINE-type AP2/ERF transcription factor of the Va subgroup was identified from N. tabacum. NtWIN1 showed high expression levels in tobacco stems, sepals, and pistils. Overexpression (OE) and knock-out of NtWIN1 showed that it promoted the accumulation of total polyphenols and altered their composition. Compare to that of WT plants, the CGA contents significantly increased by 25%–50% in the leaves, flowers, and capsules of OE lines, while the scopoletin contents in the OE plants significantly decreased by 30%–67%. In contrast, the CGA contents in ntwin1 lines reduced by 23%–26%, and the scopoletin contents in ntwin1 increased by 38%–75% compare to that of WT plants. Chromatin immunoprecipitation and Dual-Luc transcription activation assays showed that NtWIN1 could bind to the promoters of NtF6′H1 and NtCCoAMT, thereby modulating their expression. The scopoletin content in ntwin1/ntf6′h1 double mutant was significantly lower than that in ntwin1 and WT plants, but showed no significant differences with that in ntf6′h1 mutant, further indicating that the inhibition of NtWIN1 on scopoletin accumulation depends on the activity of NtF6′H1. Our study illustrates the new roles of NtWIN1, and provides a possible target for regulating the synthesis of polyphenols in tobacco.

Secondary Metabolite Production In Plants: In Response To Biotic And Abiotic Stress Factors

Secondary metabolites (SMs) play vital roles in plant defence mechanisms, adaptation to environmental conditions, and interactions with other organisms. Biotic and abiotic stress factors can significantly influence the production, accumulation, and composition of SMs in plants. Understanding the intricate relationship between stress and SM production is crucial for enhancing plant resilience, agricultural productivity, and the development of novel phytopharmaceuticals. This research provides current knowledge regarding the impact of biotic and also abiotic stress on SMs in plants. Biotic stress factors such as pathogen infection, and herbivore attacks, as well as abiotic stress factors like drought, along with temperature extremes, and also salinity, can profoundly influence the biosynthesis and accumulation of SMs in plants. We discussed the methodology based on secondary sources underlying physiological, biochemical, and molecular mechanisms involved in stress-induced SM synthesis and highlight the potential implications for plant biology, agriculture, and human health. The study also emphasizes the functions of SMs in plants including defence against herbivores, pathogens, and abiotic stresses. The mechanism by which thesecompounds act as allelochemicals and signalling molecules is also discussed.

Nutritional and Physicochemical Properties of Wild Lingonberry (Vaccinium vitis-idaea L.)-Effects of Geographic Origin.

In recent years, much attention has been devoted to Vaccinium L. berries because of their substantial potential to be adapted for the development of innovative food and pharmaceutical applications. The accumulation of plant secondary metabolites is extremely dependent on climate and other environmental conditions. In order to increase the reliability of the findings, this study was conducted with samples collected in four regions in Northern Europe (Norway, Finland, Latvia, and Lithuania) and analyzed in a single laboratory using a standardized methodology. The study aims to provide a comprehensive understanding of the nutritional (biologically active compounds (phenolic (477-775 mg/100 g fw), anthocyanins (20-57 mg/100 g fw), pro-anthocyanidins (condensed tannins (141-269 mg/100 g fw)) and antioxidant activity in different systems (ABTS•+, FRAP). Physicochemical properties (acidity, soluble solids, color) of wild Vaccinium vitis-idaea L. were also evaluated. The results may contribute to the development of functional foods and nutraceuticals with potential health benefits in the future. To the best of our knowledge, this is the first comprehensive report on the evaluation of the biologically active compounds of wild lingonberries from different Northern European countries based on one laboratory's validated methods. The results indicated a geomorphological influence on the biochemical and physicochemical composition of wild Vaccinium vitis-idaea L. depending on their place of geographical origin.

Regulation of the regulators: Transcription factors controlling biosynthesis of plant secondary metabolites during biotic stresses and their regulation by miRNAs.

Biotic stresses threaten to destabilize global food security and cause major losses to crop yield worldwide. In response to pest and pathogen attacks, plants trigger many adaptive cellular, morphological, physiological, and metabolic changes. One of the crucial stress-induced adaptive responses is the synthesis and accumulation of plant secondary metabolites (PSMs). PSMs mitigate the adverse effects of stress by maintaining the normal physiological and metabolic functioning of the plants, thereby providing stress tolerance. This differential production of PSMs is tightly orchestrated by master regulatory elements, Transcription factors (TFs) express differentially or undergo transcriptional and translational modifications during stress conditions and influence the production of PSMs. Amongst others, microRNAs, a class of small, non-coding RNA molecules that regulate gene expression post-transcriptionally, also play a vital role in controlling the expression of many such TFs. The present review summarizes the role of stress-inducible TFs in synthesizing and accumulating secondary metabolites and also highlights how miRNAs fine-tune the differential expression of various stress-responsive transcription factors during biotic stress.

Plant Secondary Metabolites as Defense Tools against Herbivores for Sustainable Crop Protection.

Plants have evolved several adaptive strategies through physiological changes in response to herbivore attacks. Plant secondary metabolites (PSMs) are synthesized to provide defensive functions and regulate defense signaling pathways to safeguard plants against herbivores. Herbivore injury initiates complex reactions which ultimately lead to synthesis and accumulation of PSMs. The biosynthesis of these metabolites is regulated by the interplay of signaling molecules comprising phytohormones. Plant volatile metabolites are released upon herbivore attack and are capable of directly inducing or priming hormonal defense signaling pathways. Secondary metabolites enable plants to quickly detect herbivore attacks and respond in a timely way in a rapidly changing scenario of pest and environment. Several studies have suggested that the potential for adaptation and/or resistance by insect herbivores to secondary metabolites is limited. These metabolites cause direct toxicity to insect pests, stimulate antixenosis mechanisms in plants to insect herbivores, and, by recruiting herbivore natural enemies, indirectly protect the plants. Herbivores adapt to secondary metabolites by the up/down regulation of sensory genes, and sequestration or detoxification of toxic metabolites. PSMs modulate multi-trophic interactions involving host plants, herbivores, natural enemies and pollinators. Although the role of secondary metabolites in plant-pollinator interplay has been little explored, several reports suggest that both plants and pollinators are mutually benefited. Molecular insights into the regulatory proteins and genes involved in the biosynthesis of secondary metabolites will pave the way for the metabolic engineering of biosynthetic pathway intermediates for improving plant tolerance to herbivores. This review throws light on the role of PSMs in modulating multi-trophic interactions, contributing to the knowledge of plant-herbivore interactions to enable their management in an eco-friendly and sustainable manner.

The Influence of Environmental Conditions on Secondary Metabolites in Medicinal Plants: A Literature Review.

Medicinal plants, a source of different phytochemical compounds, are now subjected to a variety of environmental stresses during their growth and development. Different ecologically limiting factors including temperature, carbon dioxide, lighting, ozone, soil water, soil salinity and soil fertility has significant impact on medicinal plants' physiological and biochemical responses, as well as the secondary metabolic process. Secondary metabolites (SMs) are useful for assessing the quality of therapeutic ingredients and nowadays, these are used as important natural derived drugs such as immune suppressant, antibiotics, anti-diabetic, and anti-cancer. Plants have the ability to synthesize a variety of secondary metabolites to cope with the negative effects of stress. Here, we focus on how individual environmental variables influence the accumulation of plant secondary metabolites. A total of 48 articles were found to be relevant to the review topic during our systematic review. The review showed the influence of different environmental variables on SMs production and accumulation is complex suggesting the relationship are not only species-specific but also related to increases and decline in SMs by up to 50 %. Therefore, this review improves our understanding of plant SMs ability to adapt to key environmental factors. This can aid in the efficient and long-term optimization of cultivation techniques under ambient environmental conditions in order to maximize the quality and quantity of SMs in plants.

Cannabinoids and Terpenes: How Production of Photo-Protectants Can Be Manipulated to Enhance Cannabis sativa L. Phytochemistry.

Cannabis sativa L. is cultivated for its secondary metabolites, of which the cannabinoids have documented health benefits and growing pharmaceutical potential. Recent legal cannabis production in North America and Europe has been accompanied by an increase in reported findings for optimization of naturally occurring and synthetic cannabinoid production. Of the many environmental cues that can be manipulated during plant growth in controlled environments, cannabis cultivation with different lighting spectra indicates differential production and accumulation of medically important cannabinoids, including Δ9-tetrahydrocannabinol (Δ9-THC), cannabidiol (CBD), and cannabigerol (CBG), as well as terpenes and flavonoids. Ultraviolet (UV) radiation shows potential in stimulating cannabinoid biosynthesis in cannabis trichomes and pre-harvest or post-harvest UV treatment merits further exploration to determine if plant secondary metabolite accumulation could be enhanced in this manner. Visible LED light can augment THC and terpene accumulation, but not CBD. Well-designed experiments with light wavelengths other than blue and red light will provide more insight into light-dependent regulatory and molecular pathways in cannabis. Lighting strategies such as subcanopy lighting and varied light spectra at different developmental stages can lower energy consumption and optimize cannabis PSM production. Although evidence demonstrates that secondary metabolites in cannabis may be modulated by the light spectrum like other plant species, several questions remain for cannabinoid production pathways in this fast-paced and growing industry. In summarizing recent research progress on light spectra and secondary metabolites in cannabis, along with pertinent light responses in model plant species, future research directions are presented.

Study on Quality Response to Environmental Factors and Geographical Traceability of Wild Gentiana rigescens Franch.

Gentiana rigescens Franch. ex Hemsl. is an important medicinal plant in China and the over exploitation of wild resources has affected its quality and clinical efficacy. The accumulation of plant secondary metabolites is not only determined by their genetic characteristics but also influenced by environmental factors. At present, many studies on evaluating the environmental conditions of its planting area are still in the qualitative stage. Therefore, it is necessary to establish a systematic evaluation method to deeply analyze the impact of environmental factors on the quality of medicinal materials and quickly verify the geographical origin. In this study, the contents of five iridoids (loganic acid, swertiamarin, sweroside, gentiopicroside and 6'-O-β-D-glucopyranosylgentiopicroside) of G. rigescens from 45 different origins (including 441 individuals) of Yunnan Province in China were analyzed by high performance liquid chromatography. Analytical procedures of one-way analysis of variance, correlation analysis, principal components analysis, and hierarchical cluster analysis were employed to interpret the correlation of iridoid content and environmental factors. Fourier transform infrared spectroscopy (FT-IR) combined with two multivariate analysis methods (partial least squares discriminant analysis; support vector machines, SVM) was used to discriminate four major producing areas (158 individuals). The combination of SVM with grid search algorithm achieved an accuracy of 100% in the test set. One-way analysis of variance showed that the contents of five iridoids in root tissues of G. rigescens varied significantly among different origins, which was also verified by the chemometrics analysis results of hierarchical cluster analysis. The results of correlation analysis indicated that the high value of altitude and precipitation were unfavorable for the accumulation of these five iridoids. A correlation between increase of temperature and iridoid accumulation was observed. This study provided a certain theoretical basis for the resource protection and development of G. rigescens based on the correlation analysis between the ecological environment factors and quality.

Plant growth-promoting rhizobacteria for in vitro and ex vitro performance enhancement of Apennines’ Genepì (Artemisia umbelliformis subsp. eriantha), an endangered phytotherapeutic plant

Apennines genepi (Artemisia umbelliformis Lam. subsp. eriantha), is a high-altitude plant endangered by illegal and indiscriminate harvesting to produce the homonymous liqueur. Even if synthetic seeds represented a valid propagation technique to respond to this threat, this technology still requires methods to improve the low germination and plantlet development rates. The aim of the present study was to evaluate the suitability of the bacterial consortium formed by Azospirillum brasilense, Gluconacetobacter diazotrophicus, Burkholderia ambifaria, and Herbaspirillum seropedicae, as an enrichment agent of Apennines Genepi synthetic seeds. To evaluate the effectiveness as an improving agent, the consortium was incorporated in the synthetic seeds, compared to a control, and followed until ex vitro cultivations. Plants were evaluated for their growth and development parameters, total phenolic and flavonoid contents, antioxidant properties, polyphenolic compounds concentrations, and volatile fractions. The results demonstrated that the investigated consortium is a good candidate as an enrichment agent in synthetic seed technology. The rhizobacterial presence induced an improved response in plant propagation and the acclimatization process. Plant quality was also enhanced, with an increased accumulation of plant secondary metabolites and higher antioxidant capacity. The investigated bacterial consortium is a good candidate as an enrichment agent in synthetic seed technology. Plant clones can be translocated for species conservation and also commercialized to reduce the illegal and undiscerning collections.

Plant growth-promoting rhizobacteria (PGPR) in Cannabis sativa ‘Finola’ cultivation: An alternative fertilization strategy to improve plant growth and quality characteristics

The massive employment of chemical fertilizers entails substantial costs for agriculture and leads to significant environmental pollution, soils depletion and crop productivity declines. The aim of this preliminary study was to evaluate the suitability of plant growth-promoting rhizobacteria (PGPR) as an alternative fertilization approach in Cannabis sativa L. ‘Finola’, one of the low-psychoactive substances industrial hemp varieties cultivated in the Abruzzo territory. The PGPR inoculum was first studied in a model system by monitoring the colonization and survival of bacteria in roots of hemp seedling grown in vitro. Following a complete randomized block design with three replicates, female plants were also cultivated in greenhouse and subjected to different cultivation conditions: (i) two different PGPR inoculum concentrations, (ii) nitrogen fertilization, and (iii) unfertilized control. At the flowering stage, plant growth parameters, main cannabinoid content, antioxidant, and total phenolic content, were assessed. In the model system experiment, scanning electron microscope (SEM) imaging revealed an excellent ability of bacteria to adhere to the surface of roots, and to colonize root vascular tissues of hemp seedlings. Under greenhouse conditions PGPR favored plant growth and development as well as plant secondary metabolites accumulation and, consequently, antioxidant capacity. In particular, the lowest PGPR concentration allowed obtaining results comparable with those induced by the recommended nitrogen fertilization. These results underline the potentiality of PGPR application in hemp plants in terms of both higher biomass accumulation and chemical composition, also meeting environmental goals such as an increase in soil biodiversity and a reduction in chemical inputs. This study represents the first step toward the potential application of PGPR in hemp cultivation and could be the base for future extensive evaluations.

Response of Plant Secondary Metabolites to Environmental Factors.

Plant secondary metabolites (SMs) are not only a useful array of natural products but also an important part of plant defense system against pathogenic attacks and environmental stresses. With remarkable biological activities, plant SMs are increasingly used as medicine ingredients and food additives for therapeutic, aromatic and culinary purposes. Various genetic, ontogenic, morphogenetic and environmental factors can influence the biosynthesis and accumulation of SMs. According to the literature reports, for example, SMs accumulation is strongly dependent on a variety of environmental factors such as light, temperature, soil water, soil fertility and salinity, and for most plants, a change in an individual factor may alter the content of SMs even if other factors remain constant. Here, we review with emphasis how each of single factors to affect the accumulation of plant secondary metabolites, and conduct a comparative analysis of relevant natural products in the stressed and unstressed plants. Expectantly, this documentary review will outline a general picture of environmental factors responsible for fluctuation in plant SMs, provide a practical way to obtain consistent quality and high quantity of bioactive compounds in vegetation, and present some suggestions for future research and development.

Effects of genotype and temperature on accumulation of plant secondary metabolites in Canadian and Australian wheat grown under controlled environments

Predictions of global increased temperature are for 1.8–4 °C by 2100. Increased temperature as an abiotic stress may exert a considerable influence on the levels of secondary metabolites in plants. These secondary metabolites may possibly exert biological activities beneficial in prevention or treatment of disorders linked to oxidative stress in human. Wheat secondary compounds in three Canadian and three Australian genotypes grown under controlled environments, in which the only changing parameter was temperature, were investigated. Kennedy and AC Navigator contained the highest amount of total phenolic acids among Australian and Canadian wheat genotypes, respectively. The total phenolic acids and total flavonoid contents of wheat genotypes increased following the increase of the growing temperature. In all the wheat genotypes, regardless of their growing temperatures, linoleic acid (C18:2n6) was measured as the main fatty acid. Significant increases in palmitic acid (C16:0) and oleic acid (C18:1n9) and significant decreases in linoleic acid (C18:2n6) and linolenic acid (C18:3n3) were observed at increased of growing temperature for all wheat genotypes. Growing temperature decreased campesterol content of wheat genotypes. Genotype and growing temperature significantly shifted the production of wheat secondary metabolites. This information might be used as a guide for breeding wheat varieties with higher antioxidant properties.

Dynamic changes in plant secondary metabolites during UV acclimation in Arabidopsis thaliana

Plants respond to environmental stress by synthesizing a range of secondary metabolites for defense purposes. Here we report on the effect of chronic ultraviolet (UV) radiation on the accumulation of plant secondary metabolites in Arabidopsis thaliana leaves. In the natural environment, UV is a highly dynamic environmental parameter and therefore we hypothesized that plants are continuously readjusting levels of secondary metabolites. Our data show distinct kinetic profiles for accumulation of tocopherols, polyamines and flavonoids upon UV acclimation. The lipid-soluble antioxidant α-tocopherol accumulated fast and remained elevated. Polyamines accumulated fast and transiently. This fast response implies a role for α-tocopherol and polyamines in short-term UV response. In contrast, an additional sustained accumulation of flavonols took place. The distinct accumulation patterns of these secondary metabolites confirm that the UV acclimation process is a dynamic process, and indicates that commonly used single time-point analyses do not reveal the full extent of UV acclimation. We demonstrate that UV stimulates the accumulation of specific flavonol glycosides, i.e. kaempferol and (to a lesser extent) quercetin di- and triglycosides, all specifically rhamnosylated at position seven. All metabolites were identified by Ultra Performance Liquid Chromatography (UPLC)-coupled tandem mass spectrometry. Some of these flavonol glycosides reached steady-state levels in 3-4 days, while concentrations of others are still increasing after 12 days of UV exposure. A biochemical pathway for these glycosides is postulated involving 7-O-rhamnosylation for the synthesis of all eight metabolites identified. We postulate that this 7-O-rhamnosylation has an important function in UV acclimation.

Nitric Oxide and Brassinosteroids Mediated Fungal Endophyte‐Induced Volatile Oil Production Through Protein Phosphorylation Pathways in Atractylodes lancea Plantlets

Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide (NO) and brassinolide (BL) in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. AL12 enhanced the activities of total protein phosphorylation, Ca²⁺-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte-induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.

Assessment of plant secondary metabolites in oil palm seedlings after being treated with calcium, copper ions and salicylic acid

The effect of calcium, copper ions and salicylic acid (SA) amendment on the incidence of basal stem rot and activity of secondary metabolites in oil palm seedlings were investigated in glasshouse study. Disease incidence (DI) in positive control (T8) was 75% at nine months after inoculation (9 MAI). However, weekly pre-immunisation with Ca2+ + Cu2+ + SA prior to inoculation significantly suppressed DI and delayed disease onset as noted in T7. In the present study, the lowest %DI was observed in T7 (15%) followed by T1, T5, T6, T3, T4 and T2. The Ca2+, Cu2+ and SA amendments were resulted in earlier and higher accumulation of plant secondary metabolites as noted in leaves, stems and root tissues in response to invasion by Ganoderma boninense. High total phenolic content concentration was detected in T7 (leaf: 233.38 ± 0.12 mg/g; stem: 132.78 ± 0.04 mg/g and root: 86.98 ± 0.28 mg/g). Similar trend was obtained in peroxidase activity, total lignin content and hydrogen peroxide scavenging activity. These results suggested that it could be due to the accumulation of phenolics, peroxidase activities, lignin content and hydrogen peroxide scavenging activities in oil palm seedling tissues which might have collectively contributed to induce resistance against G. boninense.

Advances in influence of UV-B radiation on medicinal plant secondary metabolism

Stratospheric ozone depletion results in an increased level of solar UV-B radiation (UV-B, 280-320 nm) reaching the earth surface. By the effect of UV-B radiation, various medicinal active ingredients changed because of the change of gene expression, enzyme activity and secondary metabolism, clinical effect is also changed. The research status of UV-B radiation and the accumulation of plant secondary metabolites in the past 10 years were summarized in this paper to supply reference for cultivation and exploitation of the medicinal plants.

Spectral dependence of flavonol and betacyanin accumulation in Mesembryanthemum crystallinum under enhanced ultraviolet radiation

Abstract Mesembryanthemum crystallinum L. (Aizoaceae) is a drought‐ and salt‐tolerant halophyte that is able to endure harsh environmental conditions. Upon irradiation with high light irradiance (1200–1500 µmol m−2 s−1) it displays a rapid cell‐specific accumulation of plant secondary metabolites in the upper leaf epidermis; a phenomenon that is not detectable with salt or drought treatment. The accumulation of these compounds, the betacyanins and acylated flavonol glycosides, increases if the plants are exposed to polychromatic radiation with a progressively decreasing short‐wave cut‐off in the ultraviolet range. The response is localized in the epidermal bladder cells on the tips of young leaves and epidermal layers of fully expanded leaves. It is demonstrated that the accumulation of flavonols and betacyanins can be described by a weakly sigmoid dose function in combination with an exponential decrease of the response function of the plant with increasing wavelength.

Are Spinach Chloroplasts Involved in Flavonoid O-Methylation?

Abstract Intact spinach chloroplasts, which were isolated on a cushion of Percoll and purified on discon­tinuous sucrose gradients, were found to adsorb appreciable amounts of high specific activity, cytosolic O-methyltransferases. The latter activity was readily eliminated after washing the chloroplast pellet with 0.1 ᴍ phosphate in sucrose gradient buffer and finally with 0 .1% nonidet. Furthermore, the OMT activity of spinach leaves was resolved by chromatography on DEAE-Sepharose CL-6B into caffeic acid (COMT) and flavonoid (FOMT) O-methyltransferase ac­tivities, and purified to 30-and 50-fold, respectively. The similarities between the FOMT/COMT activity ratios and the methylation patterns of intact chloroplasts and their supernatants, as well as those of purified leaf preparations, suggest non-specific binding of cytosolic OMTs to chloroplast envelopes. It is concluded, therefore, that spinach chloroplasts are not involved in the methylation of phenylpropanoid or flavonoid compounds. These results call attention for the re-evaluation of the role of this organelle in the biosynthesis or accumulation of plant secondary metabolites.