Accumulation Of Macronutrients Research Articles

Influence of Bacterial Nanocellulose Consumption on the Content of Macronutrients and Trace Elements in the Organs of Rats.

Bacterial nanocellulose (BNC) prepared by the methods of "green" bionanotechnological synthesis is considered a promising food additive and food ingredient. At the same time, the risk of reducing the bioavailability of minerals due to their adsorption on BNC fibers having a high specific surface area and high adsorption and ion exchange capacity cannot be excluded. We studied the effect of oral administration of BNC on the accumulation of macronutrients and trace elements included in the diet in the liver and kidneys of laboratory animals. Male Wistar rats received BNC at doses of 0 (control), 1, 10, and 100 mg/kg body weight as part of their diet for 8 weeks. The content of 30 macronutrients and trace elements in the liver and kidneys was determined by inductively coupled plasma mass spectrometry. It was found that BNC at all doses did not significantly change the content of the main essential macronutrients and trace elements in the organs (Ca, Cr, Cu, Fe, K, Mg, Mn, Na, P, Se, and Zn), which indicates the absence of a negative effect on their bioavailability. Among other elements, a statistically significant decrease in the content of As, B, Cd, Co, and Pb in the liver and an increase in Al, B, Ba, Ni, and Pb in the kidneys were revealed (more than 20% of the control). The revealed decrease in the bioaccumulation of cobalt can indicate inhibition of assimilation of certain chemical forms of this trace element under the action of BNC.

Biofortifying multiple micronutrients and decreasing arsenic accumulation in rice grain simultaneously by expressing a mutant allele of OAS-TL gene.

Rice grains typically contain relatively high levels of toxic arsenic (As) but low levels of essential micronutrients. Biofortification of essential micronutrients while decreasing As accumulation in rice would benefit human nutrition and health. We generated transgenic rice expressing a gain-of-function mutant allele astol1 driven by the OsGPX1 promoter. astol1 encodes a plastid-localized O-acetylserine (thiol) lyase (OAS-TL) with Ser189Asn substitution (OsASTOL1S189N), which enhances cysteine biosynthesis by forming an indissociable cysteine synthase complex with its partner serine acetyltransferase (SAT). The effects on growth, As tolerance, and nutrient and As accumulation in rice grain were evaluated in hydroponic, pot and field experiments. The expression of OsASTOL1S189N in pOsGPX1::astol1 transgenic lines enhanced SAT activity, sulphate uptake, biosynthesis of cysteine, glutathione, phytochelatins and nicotianamine, and enhanced tolerance to As. The expression of OsASTOL1S189N decreased As accumulation while increased the accumulation of multiple macronutrients (especially sulphur, nitrogen and potassium) and micronutrients (especially zinc and selenium) in rice grain in a pot experiment and two field experiments, and had little effect on plant growth and grain yield. Our study provides a new strategy to genetically engineer rice to biofortify multiple essential nutrients, reducing As accumulation in rice grain and enhancing As tolerance simultaneously.

Accumulation and exportation of macronutrients by peanut crops under pulse irrigation with brackish water

Accumulation and exportation of macronutrients by peanut crops under pulse irrigation with brackish water

Targeting candidate genes for the macronutrient accumulation of wheat grains for improved human nutrition

Targeting candidate genes for the macronutrient accumulation of wheat grains for improved human nutrition

Grain productivity and nutrient accumulation in millet straw grown in the off-season

Given the growing demand for grains cultivation in the off-season, an experiment was conducted to evaluate grain yield and nutrient accumulation in straw, straw production, and decomposition of pearl millet varieties and hybrids cultivated at different sowing times in off-season in the municipality of Rio Verde, state of Goiás. This was a randomized block experimental design, with four replications and four planting times. The work was carried out on an Oxisol, using two forage varieties, ADR300 and ADR500, the dual-purpose pearl millet hybrid ADR8010 for grain and biomass production, and two grain hybrids, ADR9010 and ADR 9020. The accumulation of macronutrients (nitrogen, phosphorus, potassium, calcium, magnesium and sulfur) was quantified in the straw at grain harvest. Hybrids ADR 9010 and ADR 9020 had the highest grain yield in the first season, with 2,626 and 2,425 kg ha-1, respectively, while the ADR 9010 hybrid showed the highest accumulation of macronutrients (except phosphorus) in the straw. Hybrid ADR 9010 is the best option for planting in the off-season with the objective of grain production, with high biomass production and accumulation of nutrients in the straw.

Genomic insights into organic acid production and plant growth promotion by different species of phosphate-solubilizing bacteria.

Bacteria can solubilize phosphorus (P) through the secretion of low-molecular-weight organic acids and acidification. However, the genes involved in the production of these organic acids are poorly understood. The objectives of this study were to verify the calcium phosphate solubilization and the production of low-molecular-weight organic acids by diverse genera of phosphate solubilizing bacterial strains (PSBS); to identify the genes related to the synthesis of the organic acids in the genomes of these strains and; to evaluate growth and nutrient accumulation of maize plants inoculated with PSBS and fertilized with Bayóvar rock phosphate. Genomic DNA was extracted for strain identification and annotation of genes related to the organic acids production. A greenhouse experiment was performed with five strains plus 150mg dm- 3 P2O5 as Bayóvar rock phosphate (BRP) to assess phosphate solubilization contribution to maize growth and nutrition. Paraburkholderia fungorum UFLA 04-21 and Pseudomonas anuradhapurensis UFPI B5-8A solubilized over 60% of Ca phosphate and produced high amounts of citric/maleic and gluconic acids in vitro, respectively. Eleven organic acids were identified in total, although not all strains produced all acids. Besides, enzymes related to the organic acids production were found in all bacterial genomes. Plants inoculated with strains UFPI B5-6 (Enterobacter bugandensis), UFPI B5-8A, and UFLA 03-10 (Paenibacillus peoriae) accumulated more biomass than the plants fertilized with BRP only. Strains UFLA 03-10 and UFPI B5-8A increased the accumulation of most macronutrients, including P. Collectively, the results show that PSBS can increase maize growth and nutrient accumulation based on Bayóvar rock phosphate fertilization.

Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency

Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency

An Evaluation of the Biometric Parameters and Chemical Composition of the Florets, Leaves, and Stalks of Broccoli Plants Grown in Different Soil Types

Soil affects plant growth and development, and it is one of the factors that determine crop yields and quality. Broccoli (Brassica oleracea var. italica Plenck) plants cv. Cezar were grown in an experiment conducted in 2021–2022 on a horticultural farm. The biometric parameters of broccoli plants were determined in the first stage. The chemical composition of the edible parts of broccoli was determined in the second stage, which involved two experimental factors. The first factor was the edible parts of broccoli: florets, leaves, and stalks. The second factor was the effect of soil type on the chemical composition of the edible parts of broccoli. Albic Luvisol (II) had a significant positive effect on floret height and the number of florets. Leaf yield was significantly lower when broccoli plants were grown in Eutric Cambisol (I) compared with Albic Luvisol (II) and degraded chernozem (III). Soil type had no significant influence on the macronutrient content of broccoli florets, leaves, and stalks, but the accumulation of macronutrients varied across the edible plant parts. The content of iron, zinc and manganese in broccoli plants was not significantly affected by soil type, but soil type exerted a significant effect on copper content.

The Ballast Effect of Terrigenous Lithogenic Particles From Rivers and Its Influence on POC Fluxes in the Ocean

AbstractLithogenic materials such as terrigenous lithogenic particles (TLP) can efficiently promote the formation and sinking of mineral‐associated marine organic matter, acting as important ballast and potentially playing an important role in the global carbon cycle. To assess the influence of TLP on fluxes of particulate organic carbon (POC) and other biogeochemical cycles, we construct TLP forcing fields based on global riverine suspended sediment data and then apply them to the Community Earth System Model, version 2 (CESM2) modified with the TLP ballasting effect term. Simulations forced by different concentrations of TLP transported in the surface ocean or along the bottom of continental shelves and slopes are conducted. When the TLP transports seaward along the bottom, simulated POC fluxes at 100 and 2,000 m decrease about 11% and 19%, respectively, for the global ocean, and about 9% and 12%, respectively, for the oceanic regions of continental margins. The initial abiotic ballast processes triggered by TLP input increase POC fluxes, causing additional removal and burial of dissolved iron in continental margins. This further enhances the accumulation of macronutrients in the upwelling regions and their advection transport to neighboring subtropical gyres, thus altering regional productivity when simulations reach quasi‐equilibrium. When consider the impacts of TLP in simulations, the simulated POC flux exhibits an increase in subtropical gyres but a decrease in tropical Pacific and mid‐high latitude regions. The present work highlights the importance of TLP in global biogeochemical cycles, suggesting that the amount of carbon sequestration might be overestimated without TLP in models.

Forage and macronutrient accumulation in grass-legume intercropping in a warm climate

Forage and macronutrient accumulation in grass-legume intercropping in a warm climate

The Effect of Various Tillage Methods and Meliorants Application against their Background on the Dynamics of Macronutrients Accumulation in the Winter Wheat Plants and their Output via Crop Yield

The article presents the results of two-year investigations related to the effect of different soil cultivation methods, application times of equal doses of mineral and organic fertilizers and bio-humus with bentonite application against their background on the dynamics of macronutrients accumulation and their removal/ output through the crop yield. Considering the changes in the winter wheat growth, development, and yield capacity under the effect of different soil tillage methods, as well as the applied fertilizers and bentonite in rainfed conditions, and also the nutrition characteristics, to get high and sustainable yield the cultivation of winter wheat should be implemented only through loosening method, while fertilization should be implemented by introducing natural rock bentonite into the soil either on the background of mineral fertilizers (N60P60K60) or bio humus (3.5 t/հa) in autumn, as a result of which the soil air, nutrition and humidity regimes improve, favorable conditions are created for the regular growth and development of the plant and 48.8-52 c/ha grain and 89-96 c/ha straw yield is ensured.

Agronomic and Genetic Strategies to Enhance Selenium Accumulation in Crops and Their Influence on Quality.

Selenium (Se) is an essential trace element that plays a crucial role in maintaining the health of humans, animals, and certain plants. It is extensively present throughout the Earth's crust and is absorbed by crops in the form of selenates and selenite, eventually entering the food chain. Se biofortification is an agricultural process that employs agronomic and genetic strategies. Its goal is to enhance the mechanisms of crop uptake and the accumulation of exogenous Se, resulting in the production of crops enriched with Se. This process ultimately contributes to promoting human health. Agronomic strategies in Se biofortification aim to enhance the availability of exogenous Se in crops. Concurrently, genetic strategies focus on improving a crop's capacity to uptake, transport, and accumulate Se. Early research primarily concentrated on optimizing Se biofortification methods, improving Se fertilizer efficiency, and enhancing Se content in crops. In recent years, there has been a growing realization that Se can effectively enhance crop growth and increase crop yield, thereby contributing to alleviating food shortages. Additionally, Se has been found to promote the accumulation of macro-nutrients, antioxidants, and beneficial mineral elements in crops. The supplementation of Se biofortified foods is gradually emerging as an effective approach for promoting human dietary health and alleviating hidden hunger. Therefore, in this paper, we provide a comprehensive summary of the Se biofortification conducted over the past decade, mainly focusing on Se accumulation in crops and its impact on crop quality. We discuss various Se biofortification strategies, with an emphasis on the impact of Se fertilizer strategies on crop Se accumulation and their underlying mechanisms. Furthermore, we highlight Se's role in enhancing crop quality and offer perspective on Se biofortification in crop improvement, guiding future mechanistic explorations and applications of Se biofortification.

Parsley production using organic fertilizers before planting and in top dressing

The objective of this research was to evaluate the effect of two organic fertilizers used before planting and in top dressing in the production of parsley. Seven treatments were evaluated, resulted from the factorial 2 x 3 + 1: two organic fertilizers (castor bean cake, and hoof and horn powder) x 3 modes of application (100% before planting; 100% in top dressing; 50% before planting and 50% in top dressing) + 1 control. The experimental design was in randomized blocks, with five replications and plots of 1 m2. Two harvests were done and in both the following characteristics were evaluated: the relative chlorophyll index ("Spad''), plant height, fresh (FW) and dry (DW) matter weight of shoot and macronutrients accumulation. There was no significant difference for the ‘’Spad” index in both harvests. For the other characteristics, the control was inferior to the other treatments. In the comparison among application modes, the treatment with castor bean cake, 100% before planting, was inferior to the other applications modes of this fertilizer for FW and DW. For hoof and horn powder, the 100% in top dressing application mode was superior to other applications. In the comparison between fertilizers, the hoof and horn powder was superior to castor bean cake in both harvests when application was done 100% before planting. The descending order of macronutrients accumulation was: K > N > Ca > P > Mg. Therefore, it is recommended to apply hoof and horn powder, 100% in top dressing application.

Relative role of soil nutrients vs. carbon availability on soil carbon mineralization in grassland receiving long-term N addition

High nitrogen (N) input often induces soil carbon (C) limitation, eutrophication of macronutrients, deficiency of base cations, and accumulation of toxic micronutrients. These changes are perceived to be critical factors in regulating soil C mineralization. Previous studies primarily focused on the individual effects of C, macronutrients, exchangeable base cations, and micronutrients on soil C mineralization. However, the relative importance of those factors in regulating soil C mineralization, especially in N-enriched ecosystems, remains unclear. To disentangle the relative contributions of aforementioned factors, lime and/or glucose were added to soils that were collected from a field experiment with historical N addition (6 years) at seven rates (0–50 g N m−2 year−1) in a grassland ecosystem. Lime and glucose were added to improve the soil C and key nutrient conditions. The responses of soil C mineralization rate to changes in soil C and macronutrients (N and P), exchangeable base cations (K+, Na+ and Mg2+), and micronutrients (Fe2+, Mn2+, Cu2+ and Zn2+) were examined. We found that lime addition decreased soil micronutrients, while glucose addition improved the soil available P and exchangeable base cations, especially at high historical N addition rates. The soil C mineralization was weakly associated with changes in soil nutrients, including the availability of N, P, exchangeable base cations, and micronutrients, which were conventionally and previously considered as the vital drivers of soil C mineralization. However, soil C mineralization strongly increased with glucose-induced enhancement of C availability and the subsequent enhancement of microbial biomass under increasing N addition rates. Based on the Structural Equation Model, the standardized total effects of C, macronutrients (N and P), base cations and micronutrients on soil C mineralization were 0.86, − 0.29, 0.15 and − 0.08, respectively. Findings from this study demonstrated that the N-induced significant changes in soil nutrients (e.g., eutrophication of N and P, base cations deficiency and accumulation of toxic macronutrients) mediated soil C mineralization, with C availability being the most critical driver for C mineralization in N-enriched soil. This study provides insight into the mechanistic understanding of the relationship between N input and terrestrial C cycling.

The effect of substances isolated from an aqueous extract of oak bark on the concentration of chemical elements in the body of broiler chickens

Relevance. A comprehensive study of the properties of plants containing phytobiotic components will make it possible to widely use plant extracts in animal feeding as biologically active additives of the latest generation based on raw materials of natural origin. The aim of the work is to study the effect of substances isolated from an aqueous extract of oak bark (BBVECD) on mineral metabolism in the body of broiler chickens.Methodology. The object of the study are broiler chickens of the Smena-8 cross. 120 heads of broiler chickens were selected for the experiment, which were divided into 4 groups by the method of analogues (n = 30). The control group received the basic ration (RR), I experimental — RR + BBVECD (1 ml/kg of live weight.), II experimental — RR + BBVECD (2 ml/kg of live weight.), III experimental — RR + BBVECD (3 ml/kg of live weight). The analysis of chemical elements in the obtained ash of the studied samples was carried out using the «Elan 9000» mass spectrometer and the «Optima 2000 V» atomic emission spectrometer.Results. The results obtained in the context of the effect of BBECD on mineral metabolism in broiler chickens should be discussed in the context of already available data that a number of compounds found in plant extracts may have the ability to chelate transition metal ions, especially Fe (II) and Cu (II), which is important. During the experiment, it was shown that the introduction of oak bark extract into the diet of broiler chickens mainly led to the accumulation of macronutrients (potassium — from 0.8 to 6.24%, magnesium — from 1.02 to 7.14%, phosphorus — from 5.31 to 7.9%) and essential trace elements in muscles (cobalt — 1.5 times, chromium — 3.67 times, lithium — 1.67 times, nickel — 4 times, silicon — 1.34 times), as well as to reduce the content of toxic and conditionally toxic elements in them: aluminum — 1.62 times, lead — 2 times. In this case, the most appropriate is the use of oak bark extract in a dose of 1 ml/kg of live weight of poultry.

Cover plants can contribute on macronutrient accumulation in agroforestry systems during off-season

Cover plants can contribute on macronutrient accumulation in agroforestry systems during off-season

Dry matter accumulation and macronutrient uptake in sugarcane varieties

The increase of the area cultivated with sugarcane has demanded information about the culture, mainly in relation to its nutritional needs. There are few studies that report the nutrient uptake in sugarcane, in addition, there are no reports of studies focused on the different periods of sugarcane growth and development. In view of the above, the objective of this research was to determine the macronutrient uptake and dry matter production in the shoots of two sugarcane varieties. The experiment was carried out in Vicentina, Mato Grosso do Sul, Brazil, during the 2017/2018 harvest, using two varieties of sugarcane (RB92579 and SP832847). The experimental design was in randomized blocks with two sugarcane varieties, nine collecting times (90, 120, 150, 180, 210, 240, 270, 300 and 330 days after planting (DAP) and 4 replications. Samples were collected from the shoots of 10 plants, which were divided into leaves, sheaths and stalks, then dried and weighed, and finally the samples were submitted to laboratory analysis to determine the nutrient concentrations in each organ. The accumulation of macronutrients in the shoots of both varieties followed the order: K > N > Ca > S>Mg > P. The export by the stalk followed the order K > N > Ca > S > P > Mg for RB92579 and K > N > Ca = P > S > Mg for SP832847. Maximum accumulation of nutrients and the maximum dry matter production occurred in the interval between 240 and 300 DAP. The variety SP832847 accumulated higher amounts of nutrients and dry matter in relation to RB92579, as well as the time required for the maximum accumulation was shorter.

Short-term cultivation limiting soil aggregate stability and macronutrient accumulation associated with glomalin-related soil protein in Eucalyptus urophylla × Eucalyptus grandis plantations

The relationship between glomalin-related soil protein (GRSP) and soil aggregation has been a hot topic of research for its close link to soil stability and quality. However, the short-term cultivation of Eucalyptus poses serious threats to soil stability and nutrient stocks, and the effects of GRSP on soil aggregate stability and macronutrient accumulation remain unclear. The aim is to clarify the potential mechanisms affecting soil aggregate stability and macronutrient accumulation in short-term Eucalyptus plantations. Five Eucalyptus urophylla × Eucalyptus grandis plantations with different cultivation periods (1–5 years) in this study were investigated, and a native evergreen broadleaf forest (0 year) was selected as control. The mean weight diameter index increased in the first 3 years and then significantly decreased during 5 years cultivation of Eucalyptus. Soil organic carbon (SOC) and total nitrogen also decreased after planting Eucalyptus for 3 years, but variation in total phosphorus was not obvious. The relative abundance of Glomeraceae and Claroideoglomeraceae decreased in the 5-year-old Eucalyptus plantations and was positively correlated with GRSP content. In pathway modeling, nutrient-acquisition enzyme activities positively affected GRSP and macronutrient content. Total GRSP (T-GRSP) had higher total effects than easily extractable GRSP on soil aggregate stability, and positively correlated with SOC in macroaggregates. Both T-GRSP and SOC had positive and direct effects on soil aggregate stability. Variance partitioning analysis further explained the contribution of GRSP and SOC to aggregate stability, particularly in >2 and 2–0.25 mm macroaggregates. Our results suggested that GRSP was directly associated with SOC content and soil aggregate stability, and was a potential key factor affecting soil aggregate stability in Eucalyptus plantations. Improving T-GRSP and SOC are efficient approaches for preventing the gradual deterioration of soil aggregate stability. Short-term cultivation should be carefully used in Eucalyptus plantations, and a new cultivation period is needed.

Foliar Selenate and Zinc Oxide Separately Applied to Two Pea Varieties: Effects on Growth Parameters and Accumulation of Minerals and Macronutrients in Seeds under Field Conditions.

Though selenium (Se) and zinc (Zn) constitute essential nutrients for human health, their deficiencies affect up to 15% and 17% of the global population, respectively. Agronomic biofortification of staple crops with Se/Zn may alleviate these challenges. Pea (Pisum sativum L.) is a nutritious legume crop that has great potential for Se/Zn biofortification. Herein, two varieties of pea (Ambassador, Premium) were biofortified via foliar application of sodium selenate (0/50/100 g of Se/ha) or zinc oxide (0/375/750 g of Zn/ha) during the flowering stage under field conditions. While no significant differences were found in Se accumulation between seed varieties upon Se treatments, selenate enhanced the accumulation of Se in the two seed varieties in a dose dependent manner. Selenium concentration was most elevated in seeds of Ambassador exposed to 100 g of Se/ha (3.93 mg/kg DW compared to the control (0.08 mg/kg DW), p < 0.001). 375 g of Zn/ha (35.7 mg/kg DW) and 750 g of Zn/ha (35.5 mg/kg DW) significantly and similarly enhanced Zn concentrations compared to the control (31.3 mg/kg DW) in Premium seeds, p < 0.001. Zinc oxide also improved accumulations of Fe, Cu, Mn, and Mg in Premium seeds. Se/Zn treatments did not significantly affect growth parameters and accumulations of soluble solids and protein in seeds. Positive and significant (p < 0.01) correlations were observed between Zn and Fe, Cu, Mn and Mg levels in Premium seeds, among others. Consuming 33 g/day of pea biofortified with Se at 50 g/ha and 266 g/day of pea biofortified with 375 g of Zn/ha could provide 100% of the RDA (55 μg) for Se and RDA (9.5 mg) for Zn in adults, respectively. These results are relevant for enhancing Se/Zn status in peas by foliar biofortification.

Are anthropogenic soils from dumpsites suitable for arable fields? Evaluation of soil fertility and transfer of potentially toxic elements to plants

Background and purposeThe fertility of anthropogenic soils developed from dumpsites used for arable fields is not well-studied. The study aimed to evaluate the fertility of anthropogenic soils from an abandoned dumpsite in Awotan, Nigeria, by measurable indicators and the bioaccessibility of elements of selected plant species.MethodsThe study adopted multi-analytical approaches to determine the signatures of the soils and further parameterized the bioaccessibility of elements to plants.ResultsThe comparatively high content of Ca and Na in the anthropogenic soil contributed to the slightly alkaline soil reaction against the slightly acidic control. The high amount of organic matter is well-indicated by the enrichment of organic C and N in the anthropogenic soil. Waste deposition significantly contributed to the high accumulation of macronutrients (P, Ca, K, S) and micronutrients (Mn, Na, Fe) sufficient for maximum plant growth and yields, with an adequate C/N ratio supporting effective mineralization. The high cation exchange capacity of the anthropogenic soil contributed to cations binding. Indiscriminate waste deposition resulted in a high accumulation of potentially toxic elements (PTEs; Cu, Zn, As, Cd, Pb) above permissible limits in agricultural soils following WHO limits. Potential effects on lives are evident in the high PTEs accumulation in roots and leaves of Chromolaenaodorata, Saccopetalumtectonum, Passiflorafoetida, and Sennasiamea. These plant species exhibited various PTEs accumulation, especially for Cd and Pb.ConclusionAlthough anthropogenic soils remained fertile, the bioaccessibility of PTEs by plants indicates potential threats to consumers of crops and herbs produced from such sites.