The study aimed to assess the agreement between OptoTab SERIES, alternating Cover Test, Modified Thorington test, and Von Graefe method in measuring heterophoria and accommodative convergence over accommodation (AC/A) ratio. In an initial step, heterophoria was assessed at both distance and near in a cohort of 76 healthy young volunteers using the previously described tests. Subsequently, to determine the AC/A ratio, near-vision measurements were repeated with +1.00 D and -1.00 D lenses. All tests were performed in a randomized order across participants under consistent conditions. Significant differences were found between the Modified Thorington test and all other tests at distance (Wilcoxon test, all p ≤ 0.001) and between Von Graefe and all other tests at near (Wilcoxon test, all p ≤ 0.005). Regarding the AC/A ratio, significant differences were observed between all methods in +1.00 D AC/A ratio, except for the Modified Thorington test vs. the alternating Cover Test (Wilcoxon test, p = 0.024). In the -1.00 D AC/A ratio, differences were observed between OptoTab POCKET and all the other tests (Wilcoxon test, all p ≤ 0.001). The results indicate that all methods are interchangeable except the Modified Thorington test at distance and Von Graefe at near. For the AC/A ratio, only the Modified Thorington test is interchangeable with the alternating Cover Test using +1.00 D lenses and all are interchangeable using -1.00 D lenses except OptoTab POCKET.
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