Acanthogobius Flavimanus Research Articles

A new and a known species of the Taeniacanthidae (Copepoda, Cyclopoida), recovered from Japanese fishes (Actinopterygii)

Abstract Anchistrotos kojimensis Do & Ho, 1983 is redescribed on the basis of a single female recovered from the original host, Acanthogobius flavimanus (Temminck & Schlegel, 1845) (Perciformes, Gobiidae), and Taeniacanthus uranoscopi sp. nov. is described based on specimens of both sexes recovered from Uranoscopus japonicus Houttuyn, 1782 (Perciformes, Uranoscopidae). The family Uranoscopidae is a new host group for the Taeniacanthidae.

Sequencing and Analysis of the Complete Mitochondrial Genome of Lentipes ikeae.

We sequenced and analyzed the complete mitochondrial genome of Lentipes ikeae and explored the phylogenetic relationships among Sicydiinae based on mitochondrial genome sequences. The complete mitochondrial genome sequence of L. ikeae was determined using the Illumina HiSeq X Ten sequencing platform, and the gene structural characteristics and base composition were analyzed. Based on the mitochondrial genome sequences of 28 Sicydiinae species published in GenBank and mitochondrial protein-coding genes (PCGs), Acanthogobius flavimanus (Gobionellinae) was selected as an outgroup to construct phylogenetic trees of Sicydiinae using the maximum likelihood and Bayesian inference methods. The mitochondrial genome of L. ikeae (GenBank number: OP764680) has a total length of 16,498 bp and encodes 13 PCGs, 22 transfer RNA genes, two ribosomal RNA genes, and a D-loop (control) region. Gene rearrangement is not observed. The mitochondrial genome of L. ikeae exhibits an AT preference, with AT skew > 0 and GC skew < 0 across the entire genome. The phylogenetic relationships of Sicydiinae based on 13 mitochondrial PCG sequences are Sicydium + (Stiphodon + (Sicyopus + Lentipes)) + Sicyopterus, indicating that Sicydium, Sicyopterus, Lentipes, and Stiphodon are all monophyletic groups.

Whole-Genome Survey Analyses of Five Goby Species Provide Insights into Their Genetic Evolution and Invasion-Related Genes.

As one of the most abundant groups in marine fish families, Gobiidae fish are important fishery resources in China, and some are also invasive species in certain regions worldwide. However, the phylogenetic relationships of Gobiidae fish remain ambiguous, and the study of their invasion-related genes is still scarce. This study used high-throughput sequencing technology to conduct a whole-genome survey of five Gobiidae fish species: Acanthogobius flavimanus, Acanthogobius stigmothonus, Favonigobius gymnauchen, Ctenotrypauchen microcephalus, and Tridentiger barbatus. De novo assembly of five fish genomes was performed, and genomic traits were compared through K-mer analysis. Among the five Gobiidae fish genomes, F. gymnauchen had the largest genome size (1601.98 Mb) and the highest heterozygosity (1.56%) and repeat rates (59.83%). Phylogenetic studies showed that A. flavimanus was most closely linked to A. stigmothonus, while Apogonidae and Gobiidae were closely related families. PSMC analysis revealed that C. microcephalus experienced a notable population expansion than the other four fish species in the Early Holocene. By using the KOG, GO, and KEGG databases to annotate single-copy genes, the annotated genes of the five fish were mainly classified as "signal transduction mechanisms", "cellular process", "cellular anatomical entity", and "translation". Acanthogobius flavimanus, A. stigmothonus, and T. barbatus had more genes classified as "response to stimulus" and "localization", which may have played an important role in their invasive processes. Our study also provides valuable material about Gobiidae fish genomics and genetic evolution.

Ascending Visual Pathways to the Telencephalon in Teleosts with Special Focus on Forebrain Visual Centers, Associated Neural Circuitries, and Evolution.

Visual pathways to the telencephalon in teleost fishes have been studied in detail only in a few species, and their evolutionary history remained unclear. On the basis of our recent studies we propose that there were two visual pathways in the common ancestor of teleosts, while one of them became lost in acanthopterygian fishes that emerged relatively recently. Our in-depth analyses on the connections of visual centers also revealed that there are connections shared with those of mammals, and retinotopic organization of the ascending connections is maintained at least to the level of the diencephalon in the yellowfin goby. The major visual telencephalic center, or the lateral part of the dorsal telencephalon (Dl), shows considerable species differences in the number of regions and cytoarchitecture. In particular, four highly specialized compartments are noted in the Dl of gobies, and we analyzed about 100 species of teleosts to investigate the evolution of the compartments in the Dl, which indicated that four compartments emerged only in Gobiiformes, while there are fewer specialized compartments in some other percomorph lineages. We also discuss the connections of forebrain visual centers with the cerebellum and other lower brain centers and infer possible functions of the circuitries.

Comparative study of Japanese eel and yellowfin goby brains using a new brain size index

Comparative study of Japanese eel and yellowfin goby brains using a new brain size index

Microbiome and Metabolome Analyses in Different Closed-Circulation Aquarium Systems and Their Network Visualization.

Understanding the causes of microbiome formation and its relationship to environmental conditions is important to properly maintain recirculating aquaculture systems (RASs). Although RAS has been applied to numerous fish types and environmental conditions (e.g., loading intensity), the effects of these environmental conditions (i.e., fish type and loading intensity) on microbiome composition are limitedly known. Therefore, we established three experimental aquarium tanks to explore the effects of fish type, loading intensity, filter pore size, and rearing day on microbiome compositions: (1) a tank for Acanthogobius flavimanus, (2) for Girella punctata, and (3) for G. punctata with higher loading intensity. Multivariate analysis showed that the microbial community composition differed among the tanks, indicating that the fish type and loading intensity significantly affected microbiome formation in rearing water. Some microbes, such as Sediminicola and Glaciecola, were detected at a higher loading intensity, indicating that these microbes might be an indicator of eutrophic conditions in the aquacultural systems. In addition, a partial correlation network revealed a connection between microbes and metabolites in the aquarium tanks. Such a microbe–metabolite network might be a clue to control the microbiome by adjusting the molecule abundance in the aquacultural environment.

Low level of microplastic contamination in wild fish from an urban estuary

Microplastic accumulation in estuarine environments is considered the dominant input of land-based plastics into the oceans. In this study, the level of microplastic contamination was evaluated in 26 species of wild fish from the Pearl River Estuary, South China. Results showed that microplastics abundance ranged from 0.17 items individual−1 (Boleophthalmus pectinirostris &Acanthogobius flavimanus) to 1.33 items individual−1 (Plectorhynchus cinctus) among different species. The distribution of microplastic abundance in the gills and gastrointestinal tracts was not significantly different. Microplastics in gills are strongly related to the filtration area of gills in 15 fish species. Fibers were the dominant shapes accounting for 93.45% of the total shapes. The majority of microplastics were <3 mm in size. The most common polymer composition was polyethylene terephthalate (38.2%) and the most common color was black (30.36%). The findings of this study provide baseline data for microplastic contamination in wild fish from an urban estuary.

Afferent and efferent connections of the nucleus prethalamicus in the yellowfin goby Acanthogobius flavimanus.

The nucleus prethalamicus (PTh) receives fibers from the optic tectum and then projects to the dorsal telencephalon in the yellowfin goby Acanthogobius flavimanus. However, it remained unclear whether the PTh is a visual relay nucleus, because the optic tectum receives not only visual but also other sensory modalities. Furthermore, precise telencephalic regions receiving prethalamic input remained unknown in the goby. We therefore investigated the full set of afferent and efferent connections of the PTh by direct tracer injections into the nucleus. Injections into the PTh labeled cells in the optic tectum, ventromedial thalamic nucleus, central and medial parts of the dorsal telencephalon, and caudal lobe of the cerebellum. We found that the somata of most tecto-prethalamic neurons are present in the stratum periventriculare. Their dendrites ascend to reach the major retinorecipient layers of the tectum. The PTh is composed of two subnuclei (medial and lateral) and topographic organization was appreciated only for tectal projections to the lateral subnucleus (PTh-l), which also receives sparse retinal projections. In contrast, the medial subnucleus receives fibers only from the medial tectum. We found that the PTh projects to nine subregions in the dorsal telencephalon and four in the ventral telencephalon. Furthermore, cerebellar injections revealed that cerebello-prethalamic fibers cross the midline twice to innervate the PTh-l on both sides. The present study is the first detailed report on the full set of the connections of PTh, which suggests that the PTh relays visual information from the optic tectum to the telencephalon.

Infection Status with Digenetic Trematode Metacercariae in Fishes from Coastal Lakes in Gangwon-do, Republic of Korea.

The infection status of digenetic trematode metacercariae (DTM) was investigated in fishes from coastal lakes in Gangwon-do, the Republic of Korea (Korea). All fishes collected in 5 lakes were examined with the artificial digestion method. More than 10 species, i.e., Metagonimus spp., Pygidiopsis summa, Centrocestus armatus, Metorchis orientalis, M. taiwanensis, Clinostomum complanatum, Echinostoma spp., Stictodora spp., Diplostomum sp. and Diplostomid No. 1. by Morita (1960), of DTM were detected in fishes from 5 coastal lakes in Gangwon-do. Metagonimus spp. metacercariae were found in 52 (41.3%) out of 126 sea rundace, Tribolodon hakonensis, from 5 lakes, and their density was 14.6 per fish infected. P. summa metacercariae were detected in 48 (84.2%) out of 57 mullets from 5 lakes, and their density was 316 per fish infected. C. armatus metacercariae were detected in 7 (14.6%) T. hakonensis and 3 (15.0%) Tridentiger brevispinis from Hyang-ho, and 5 (19.2%) Acanthogobius flavimanus from Gyeongpo-ho. Stictodora spp. metacercariae were found in 4 fish species, i.e., Tridentiger obscurus, Tridentiger trigonocephalus, Chelon haematocheilus, and Acanthogobius lactipes, from Gyeongpo-ho. Total 15 C. complanatum metacercariae were detected in 2 (9.1%) crucian carp, Carassius auratus, from Songji-ho. M. taiwanensis metacercariae were found in T. hakonensis from Hyang-ho and Gyeongpo-ho and in Pseudorasbora parva from Gyeongpo-ho. Total 11 M. orientalis metacercariae were detected in 3 (6.3%) T. hakonensis from Hyang-ho. From the above results, it was confirmed that various species of DTM are infected in fishes from coastal lakes in Gangwon-do, Korea.

Field survey of environmental estrogen pollution in the coastal area of Tokyo Bay and Nagasaki City using the Japanese common goby Acanthogobius flavimanus

Endocrine disrupting chemicals (EDCs) are common pollutants in coastal waters. To investigate the estrogen risk of EDCs in the coastal areas of Japan, the Japanese common goby, which is a commonly observed species in these waters, was used as the target fish. Plasma 17β-estradiol (E2) and vitellogenin (VTG) levels were analyzed and the gonads of fish collected from the Taira River (northern Nagasaki, reference site), Nagasaki Port, and two sites in Tokyo Bay were observed. Abnormal levels (>150 ng/mL, p < 0.05) of plasma VTG and high levels of plasma E2 were detected in the fish from Nagasaki Port and Tokyo Bay, whereas the levels of both were low in the fish from the Taira River. The target EDCs, including natural estrogen [estrone (E1), and E2] and alkylphenols [4-t-octylphenol (4-t-OP), 4-nonylphenol (4-NP), and bisphenol-A (BPA)] in water samples were quantified using gas chromatography tandem mass spectrometry (GC/MS/MS), respectively. It was observed that the E2-equivalent (EEQ) in Nagasaki Port and Tokyo Bay, which was calculated from the actual EDC measurement value, were almost 20- and 150-fold higher, respectively, than that at the reference site (Taira River, 0.021 ng/L). The EEQs mostly comprised natural estrogen in the sampling sites, although there was some influence of alkylphenols. There was an association between the EEQ and the E2 in environmental water, suggesting a high estrogen risk in Japan coastal waters. Moreover, the results indicated that abnormal VTG synthesis was induced by environmental estrogen (EE) pollution in Nagasaki Port and Tokyo Bay.

낙동강 하구역에 출현하는 문절망둑 (Acanthogobius flavimanus) 자치어의 식성

낙동강 하구역에 출현하는 문절망둑 (Acanthogobius flavimanus) 자치어의 식성

Genetic isolation by distance in the yellowfin goby populations revealed by RAD sequencing

Population genetic studies of marine species sometimes find no clear patterns of genetic differentiation, but signals of geographic gene flow typified by isolation by distance (IBD) due to high dispersal ability. A previous study of mitochondrial DNA of the yellowfin goby Acanthogobius flavimanus indicated that this species also does not have clearly differentiated population groups around the Japanese archipelago, in contrast with other coastal goby species that have highly differentiated groups between the Sea of Japan and Pacific Ocean populations. Here, to clearly reveal phylogeographic structures of the yellowfin goby around the Japanese archipelago, we conducted large-scale RAD-sequencing analysis of their nuclear genomic SNP loci. As a result, IBD patterns were confirmed across the yellowfin goby populations, and the larval dispersal distance of this species was revealed to be approximately 19 km on average. In addition, different larval dispersal distances were estimated for its Sea of Japan and Pacific Ocean populations, suggesting that different coastal environments and/or ecological features may affect its dispersal rates. These results would provide foundations for ecological and conservation studies of the yellowfin goby and coastal fishes having similar dispersal strategy to this species.

거문도 연안에서 저인망에 어획되는 어류의 종조성 및 양적 변동

The fishes were caught by otter trawl from November, 2007 to October, 2008 in coastal waters off Geomundo. A total of 4,645 fishes were sampled and classified into 76 species, 40 families, and 12 orders. Of the 12 orders, Clupeiformes, Gadiformes, Perciformes accounted for approximately 83.7% of the fishes in this area. Of 76 species identified, Argyrosomus argentatus, Acanthogobius flavimanus, Nuchequula nuchalis, Coelorinchus japonicus, Engraulis japonicus accounted for 54.6% of the fishes collected. The highest number of individuals was found in summer, while the lowest in autumn. The richness index was highest in spring(RI=7.934) and lowest in winter(RI=6.701). The eveness index was highest in spring(E=0.740) and lowest in winter(E=0.690). The diversity index was highest in spring(H’=3.005) and lowest in winter(H’=2.671).

A lambda‐shaped retractor lentis muscle in the yellowfin goby Acanthogobius flavimanus

We identified a morphologically uncommon piscine retractor lentis muscle in the yellowfin goby Acanthogobius flavimanus. This lentis muscle has a shape similar to the Greek small letter lambda (λ). The two legs of the muscle are attached to the retinal periphery at the ventral eyecup, while the tip is connected to the lens surface by a ligament. Scanning electron microscopy showed that the fibers of the lentis muscle run along the length of both the anterior and posterior legs. Immunolabeling with antiacetylated tubulin antibody and neuronal tracing with DiI of the whole lentis muscle revealed that the anterior leg is innervated by one or more nerves. The topographic distribution of ganglion cells in the retina was investigated to identify the visual axis. Three high cell density areas were observed in the dorso-temporal, ventro-nasals and ventro-temporal retina. These findings suggest that the λ-shaped lentis muscle may enable accommodatory movement of the lens toward the temporal as well as the nasal and/or ventral retina.

Changes in plasma vitellogenin and estradiol-17β levels during the gonadal development of the female Japanese common goby Acanthogobius flavimanus

Changes in plasma vitellogenin and estradiol-17β levels during the gonadal development of the female Japanese common goby Acanthogobius flavimanus

경포호도립공원 어류상

Gyeongpoho Provincial Park some off after to clarify change fish fauna and fish community in the lagoon Gyeongpoho Provincial Park, the author surveyed fish fauna at 5 stations from August to October, 2018. The collected species during the surveyed period were 13 species of 7 families in the lagoon Gyeongpoho, 8 species of 4 families in the Sunpo swamp, and total 18 species and 324 individual belonging to 9 families. National monument species, endangered species and Korean endemic species were not found. The fishes distributed in the blackish water and sea water were abundant in 7 (38.9%) species such as <italic>Thryssa kammalensis</italic>, <italic>Tribolodon hakonensis</italic>, <italic>Mugil cephalus</italic>, <italic>Acanthogobius lactipes</italic>, <italic>Acanthogobius flavimanus</italic>, <italic>Tridentiger brevispinis</italic>, and <italic>Takifugu niphobles</italic>. The exotic species, <italic>Micropterus salmoides</italic>, first appeared in the lagoon Gyeongpoho. Dominant species were <italic>Carassius auratus</italic> (St. 1), <italic>Tridentiger brevispinis</italic> (St. 2), and <italic>Acanthogobius flavimanus</italic> (St. 3) in the lagoon Gyeongpoho amd <italic>C. auratus</italic> (St. 4, 5) in the Sunpo swamp. The each station per fish community diversity index was low, the condition was unstable dominant index and evenness index increases.

Morphological and molecular data for species of Lecithaster Lühe, 1901 and Hysterolecithoides Yamaguti, 1934 (Digenea: Lecithasteridae) from fish of East Asia and phylogenetic relationships within the Hemiuroidea Looss, 1899.

Four representatives of the genus Lecithaster and one representative of the genus Hysterolecithoides were found during investigation of the trematode fauna of fish species in Vietnamese, Japanese and eastern coastal waters of the Russian Far East. Based on morphometric data, adult trematodes from Vietnamese Strongylura strongylura and Russian Acanthogobius flavimanus were identified as Lecithaster confusus, trematodes from Vietnamese Hemirhamphus marginatus as L. sayori and from osmerid fishes as L. salmonis. Further, a single specimen of Lecithaster sp. and representatives of Hysterolecithoides epinepheli were found in Vietnamese Siganus fuscescens. Morphological and molecular data, including 18S ribosomal DNA (rDNA) V4 fragment, 28S rDNA D1-D3 fragment, internal transcribed spacers (ITS) and a mitochondrial COI gene fragment were analysed for Lecithaster spp. The results revealed that L. sayori and L. salmonis are not synonyms of L. stellatus and L. gibbosus, respectively, but that Hysterolecithoides frontilatus and H. guangdongensis are junior synonyms of H. epinepheli. The 28S-rDNA-based phylogenetic tree of Hemiuroidea showed a distinct position for the genus Lecithaster with internal differentiation into three subclades, including L. confusus, L. sayori and Lecithaster sp. within the first subclade, L. mugilis and L. sudzuhensis within the second subclade and L. salmonis and L. gibbosus within the third subclade. Bayesian phylogenetic reconstructions of Hemiuroidea showed four clades for members of Hemiuridae and Lecithasteridae. The first clade consisted of Hemiuridae representatives and the second clade represented the genus Lecithaster. The third clade included genera Aponurus and Lecithophyllum (Lecithasteridae) and the fourth clade combined members of lecithasterid Quadrifoliovariinae and Hysterolecithinae and hemiurid Opisthadeninae and Bunocotylidae with high statistical support.

CHANGES IN COMPOSITION OF FISH AND DECAPODS CATCHES ALONG SALINITY GRADIENT IN THE RAZDOLNAYA RIVER AND TOP OF THE AMUR BAY IN THE WARM PERIOD OF YEAR

Quantitative samples of fish, crabs and shrimps from the Razdolnaya estuary are analyzed. The area surveyed in May-September of 1990–2014 includes the internal estuary of this largest river of southern Primorye and its external estuary — the top of the Amur Bay with the depth &lt; 13 m. In total, 478 samples were collected: 244 in the external estuary by trawl and 234 in the internal estuary by fry seine. Distribution density and biomass are calculated using the «areal» method with certain coefficients of catchability (1.0 for fry seine). The upper freshwater layer (salinity 0–5 ‰) was observed in the lower stream of the river till sandy bar, the brackish-water cline was traced at the river bottom from the mouth to the distance 10–15 km upstream. The seawater with salinity &gt; 30 ‰ did not penetrate over sand bar to the river. Species diversity of fish and decapods was presented by 108 taxa (fishes — 88, decapods — 20) belonged to 43 families, among them 84 species were caught in the Amur Bay and 41 species in the Razdolnaya, but no more than 67 species were caught in the Amur Bay only and 24 species — in the Razdolnaya only, other 17 species were found in both external and internal estuaries (Acanthogobius flavimanus, Acanthogobius lactipes, Crangon septemspinosa, Crangon spp., Eriocheir japonica, Hypomesus nipponensis, Giporhamphus sajori, Konosirus punctatus, Liopsetta pinnifasciata, Oncorhynchus masou masou, Palaemon sp., Pholis nebulosa, Planiliza haematocheila, Pugettia quadridens, Salangichthys microdon, Syngnathus schlegeli and Tribolodon spp.). Total biomass was higher (9.1 g/m2 ) in the external estuary than in the internal estuary (6.7 g/m2 ) with the mean value 9.0 g/m2 . It was almost uniform over the internal estuary with only slight increasing on the distance about 5 km from the mouth. Simpson index of dominance increases from external to internal estuary but decreases in the river in the order: 0.127 at Peschany Peninsula — 0.168 in the northwestern Amur Bay — from 0.193 to 0.252 in the internal estuary — 0.164 in the river. Six clusters with similar species composition are defined, they change in direction from the sea to the river in the following order of the dominant species: L. pinnifasciata in the sea — prawns gen. Palaemon at the bar (upper part of the external estuary) — P. haematocheila at the river mouth (lower part of the internal estuary) — P. haematocheila and E. japonica in the main part of the internal estuary — Acanthorhodeus chankaensis and Gobio macrocephalus (with the highest biomass up to 22 g/m2 ) in the minor adjoining river stream — G. macrocephalus in the upper part of the internal estuary.

Seagrass fish assemblages in the Namhae Island, Korea: The influences of seagrass vegetation and biomass

Seagrass habitats are highly productive areas that support vegetative habitats and provide shelter for various marine animals. In seagrass beds, vegetation patterns are important factors that control faunal assemblage structure. In this study, we surveyed fish assemblage structures in Zostera marina beds and in adjacent unvegetated habitats to assess the influences of seagrass vegetation and biomass on fish assemblage structures. In total, fifty-four fish species belonging to 30 families were collected using a small beam trawl at both seagrass beds and unvegetated habitats from two different areas off the coast of Namhae Island, South Korea. The dominant fish species in seagrass beds were Sebastes inermis and Rudarius ercodes, whereas Acanthogobius flavimanus and Takifugu niphobles were the most abundant fish species in unvegetated habitats. The number of species, their abundance, and the diversity of fish assemblages varied greatly by habitat type, study site, and season. Abundance peaked in seagrass beds and during the spring, and were lowest catch rate in unvegetated habitats and during the winter months. Multivariate analyses revealed that habitat type, site, and season affected the structure of fish assemblages. Seagrass vegetation supported higher densities and greater fish species richness, but not species diversity. Among the dominant species, Lateolabrax japonicus, Sebastes schlegelii, Syngnathus schlegeli, and Rudarius ercodes characterized the fish assemblages in seagrass beds, with the former two more abundant under conditions of higher seagrass biomass. Gobiid species were significantly correlated with unvegetated habitats.

Infections with Zoonotic Trematode Metacercariae in Yellowfin Goby, Acanthogobius flavimanus, from Coastal Areas of Republic of Korea.

To investigate the infection status of zoonotic trematode metacercariae in yellowfin goby, Acanthogobius flavimanus, from coastal areas of the Republic of Korea (Korea), we examined total 344 gobies from 21 localities with an artificial digestion method from 2013 to 2017. The metacercariae of Stictodora lari were most frequently detected in 45.3% goby from 7 localities, i.e., Taean-gun (Chungcheongnam-do), Gochang-gun (Jeollabuk-do), Muan-gun, Shinan-gun, Haenam-gun (Jeollanam-do), Hadong-gun and Goseong-gun (Gyeongsangnam-do). Their infection rates were 90.0%, 66.7%, 46.7%, 8.0%, 3.3%, 26.7%, and 86.7% and intensities were 1,090, 6.2, 1.6 1.0, 2.0, 2.0, and 7.2 metacercariae per fish infected respectively. Heterophyopsis continua metacercariae were found in 38.2% goby from 6 localities, i.e., Gochang-gun, Muan-gun, Shinan-gun, Gangjin-gun, Boseong-gun (Jeollanam-do) and Goseong-gun, and their intensities were relatively low, 1–21 metacercariae. Stictodora fuscata metacercariae were detected in 61.3% goby from 4 localities, i.e., Taean-gun, Gochang-gun, Hadong-gun and Goseong-gun. Their infection rates were 90.0%, 53.3%, 5.9%, and 73.3% and intensities were 1,081, 3.1, 3.0, and 10.2 metacercariae per fish infected respectively. Heterophyes nocens metacercariae were found in 55.0% goby from Muan-gun and Shinan-gun. Total 3 metacercariae of Isthmiophora hortensis were detected in 2 (8.0%) gobies from Shinan-gun. Total 15 metacercariae of Centrocestus armatus were detected in 5 gobies (33.3%) from Gyeongpo-ho (ho means lake) in Gangneung-si, Gangwon-do. The present study suggests that yellowfin goby, A. flavimanus, acts as the infection sources of zoonotic intestinal flukes in western and southern coastal areas than in eastern coastal areas of Korea.