Academy Of Sciences Research Articles

A Bibliometric Analysis on Research Progress of Earthworms in Soil Ecosystems

The earthworm, as a soil engineer, plays highly important roles in the soil ecosystem for shaping soil structure, promoting soil fertility, regulating microbial community composition and activities and decomposing soil pollutants. However, the research progresses on this important soil fauna have rarely been reviewed so far. Therefore, we conducted a bibliometric analysis of the literature published during 1900–2022, which was collected from the Web of Science Core Collection (WoS). The results showed that three periods (1900–1990, 1991–2005 and 2006–2022) could be identified in terms of the intensity of publications on the topic, and the number of publications kept increasing since 2006. The United States produced the highest publication record at the country scale, whereas Chinese Academy of Sciences was the most productive institution. Chinese institutions and authors played an active and prominent role during 2018–2022. Soil Biology & Biochemistry was the most popular journal for the topic-related research. In these publications, Professor Lavelle P was the most influential author. Based on a citation network of the top 50 cited papers, four hotspots were identified, i.e., the ecological effects of earthworms, the impact of agricultural activities on earthworms, earthworm ecotoxicology and earthworm invasion. Moreover, “impact”, “biodiversity”, “oxidative stress”, “diversity”, “response”, “Eisenia fetida” and “exposure” were the emerging and active topics in recent years. This study can help us to better understand the relevant subject categories, journals, countries, institutions, authors and articles and identify the research hotspots and emerging trends in the field of soil earthworm research.

Mesenchymal-epithelial transition factor amplification correlates with adverse pathological features and poor clinical outcome in colorectal cancer

BACKGROUND Colorectal cancer (CRC) is the third most common cancer and the second most common cause of cancer-related mortality worldwide. Mesenchymal-epithelial transition factor (MET ) gene participates in multiple tumor biology and shows clinical potential for pharmacological manipulation in tumor treatment. MET amplification has been reported in CRC, but data are very limited. Investigating pathological values of MET in CRC may provide new therapeutic and genetic screening options in future clinical practice. AIM To determine the pathological significance of MET amplification in CRC and to propose a feasible screening strategy. METHODS A number of 205 newly diagnosed CRC patients undergoing surgical resection without any preoperative therapy at Shenzhen Cancer Hospital of Chinese Academy of Medical Sciences were recruited. All patients were without RAS/RAF mutation or microsatellite instability-high. MET amplification and c-MET protein expression were analyzed using fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), respectively. Correlations between MET aberration and pathological features were detected using the chi-squared test. Progression free survival (PFS) during the two-year follow-up was detected using the Kaplan-Meier method and log rank test. The results of MET FISH and IHC were compared using one-way ANOVA. RESULTS Polysomy-induced MET amplification was observed in 14.4% of cases, and focal MET amplification was not detected. Polysomy-induced MET amplification was associated with a higher frequency of lymph node metastasis (LNM) (P < 0.001) and higher tumor budding grade (P = 0.02). In the survival analysis, significant difference was detected between patients with amplified- and non-amplified MET in a two-year follow-up after the first diagnosis (P = 0.001). C-MET scores of 0, 1+, 2+, and 3+ were observed in 1.4%, 24.9%, 54.7%, and 19.0% of tumors, respectively. C-MET overexpression correlated with higher frequency of LNM (P = 0.002), but no significant difference of PFS was detected between patients with different protein levels. In terms of concordance between MET FISH and IHC results, MET copy number showed no difference in c-MET IHC 0/1+ (3.35 ± 0.18), 2+ (3.29 ± 0.11) and 3+ (3.58 ± 0.22) cohorts, and the MET -to-CEP7 ratio showed no difference in three groups (1.09 ± 0.02, 1.10 ± 0.01, and 1.09 ± 0.03). CONCLUSION In CRC, focal MET amplification was a rare event. Polysomy-induced MET amplification correlated with adverse pathological characteristics and poor prognosis. IHC was a poor screening tool for MET amplification.

Evolution of State Scientific and Technical Policy in USSR in Late 1960s

This study conducts a comparative analysis of the state of the scientific and technical complex of the USSR and leading Western countries in the 1960s based on declassified archival materials. It identifies the main trends in science and technology during this period and the factors that led to the lagging behind of the USSR. Previously unpublished archival documents are introduced into scholarly discourse. It is demonstrated that the data sent to the country’s leadership by leading specialists and scientists of the Academy of Sciences indicated the USSR’s lag in several crucial directions determining the pace of scientific and technical progress. Key hindering factors cited by experts included insufficient funding, lack of coordination among various agencies, absence of interaction between the defense and civilian sectors of science, and planning miscalculations. The conclusion is drawn that this list can be supplemented with reasons such as inadequate competence of some top-level government officials, departmental approach, shortage of specialists in key areas, and disregard for recommendations from leading scientists. Meanwhile, trends in the development of the scientific and technical sphere in the USA and other leading countries indicated the establishment of national innovation systems as highly effective mechanisms for accelerating scientific and technical progress.

Human epidermal growth factor receptor 2 expression level and combined positive score can evaluate efficacy of advanced gastric cancer

BACKGROUND Although treatment options for gastric cancer (GC) continue to advance, the overall prognosis for patients with GC remains poor. At present, the predictors of treatment efficacy remain controversial except for high microsatellite instability. AIM To develop methods to identify groups of patients with GC who would benefit the most from receiving the combination of a programmed cell death protein 1 (PD-1) inhibitor and chemotherapy. METHODS We acquired data from 63 patients with human epidermal growth factor receptor 2 (HER2)-negative GC with a histological diagnosis of GC at the Cancer Hospital, Chinese Academy of Medical Sciences between November 2020 and October 2022. All of the patients screened received a PD-1 inhibitor combined with chemotherapy as the first-line treatment. RESULTS As of July 1, 2023, the objective response rate was 61.9%, and the disease control rate was 96.8%. The median progression-free survival (mPFS) for all patients was 6.3 months. The median overall survival was not achieved. Survival analysis showed that patients with a combined positive score (CPS) ≥ 1 exhibited an extended trend in progression-free survival (PFS) when compared to patients with a CPS of 0 after receiving a PD-1 inhibitor combined with oxaliplatin and tegafur as the first-line treatment. PFS exhibited a trend for prolongation as the expression level of HER2 increased. Based on PFS, we divided patients into two groups: A treatment group with excellent efficacy and a treatment group with poor efficacy. The mPFS of the excellent efficacy group was 8 months, with a mPFS of 9.1 months after excluding a cohort of patients who received interrupted therapy due to surgery. The mPFS was 4.5 months in patients in the group with poor efficacy who did not receive surgery. Using good/poor efficacy as the endpoint of our study, univariate analysis revealed that both CPS score (P = 0.004) and HER2 expression level (P = 0.015) were both factors that exerted significant influence on the efficacy of treatment the combination of a PD-1 inhibitor and chemotherapy in patients with advanced GC (AGC). Finally, multivariate analysis confirmed that CPS score was a significant influencing factor. CONCLUSION CPS score and HER2 expression both impacted the efficacy of immunotherapy combined with chemotherapy in AGC patients who were non-positive for HER2.

ЖЕНА И ПОМОЩНИЦА ГУБЕРНАТОРА: А.М. АРЦИМОВИЧ В СИБИРИ (1854–1858 гг.)

The paper examines the problem of the significance and possibility of studying the biographies of the wives of high-ranking officials of the Russian Empire. The wives of high-ranking officials, including figures of the Siberian administration, did not officially work, but played an important role in public life and management practices. Sometimes they did not abandon traditions, but adopted the norms of the new era. A review of the research literature allowed the author to note that, despite the impressive number of works by domestic and foreign historians on gender issues, the issue has not been sufficiently studied. The use of memoir-epistolary sources created by the wife of the Tobolsk governor V.A. Artsimovich, her relatives and acquaintances, made it possible to fill this gap using the example of one of these ladies. For the first time, letters from A.M. Artsimovich and V.A. Artsimovich to her are being introduced into scientific circulation from the funds of V.A. Artsimovich in the State Archives of the Russian Federation and the Manuscript Department of the Institute of Russian Literature of the Russian Academy of Sciences. The main stages of the biography of Anna Mikhailovna Artsimovich (nee Zhemchuzhnikova) are reconstructed, the daily life of the governor’s family, its complexities and specifics in Siberian conditions are characterized. The main directions and features of the activities of the governor’s wife and assistant, participation as a trustee in the management of the Mariinsky Girls’ School are shown. The significance of the Siberian years for the development of the personality of an extraordinary woman was revealed. The evolution of her views, her transformation into a like-minded person and ideological ally of the famous statesman of the era of the Great Reforms is covered. An important result of the study was the filling of the gap in characterizing the role in public life of the wives of prominent figures in the Siberian administration.

Building Integrated Photovoltaics (BIPV): Analysis of the Technological Transfer Process and Innovation Dynamics in the Swiss Building Sector

Solar has confirmed its dominance among all power generation technologies, and along with the demand for zero-emission buildings, Photovoltaics (PV) is contributing to transforming the building skin. More than 200 products for Building Integrated Photovoltaics (BIPV) are commercialized nowadays in the EU market. However, only 1–3% of all PV installations are BIPV due to the weak penetration in the construction sector. At the state of the art, the sector lacks a specific analysis from a construction technology perspective, describing the dynamics and the traits that BIPV innovation articulates on construction and architectural processes. The authors, elaborating a new model from the building technology sector to explore the relationship between PV technology and architectural innovation, aim to identify the main principles, forms, and approaches that describe the structural organization of the “integrability” concept of PV in buildings. This study applies the method to a database of 233 real buildings located in Switzerland, a unique country leading with a 10% BIPV rate on PV installations documented between the years 1997 and 2023. The novel findings of the research suggest the definition of the levels of innovation and the reference traits of the innovative process of BIPV in the Swiss construction sector, which can also be used in other practical applications and contexts. The results of the paper are expected to impact both the scientific academy and the key players from the construction sector, encouraging the adoption of an integrated research and design approach to revolutionize the energy role of building skins with PV.

Imaging characteristics and diagnosis of primary middle mediastinal tumors

Objectives: To investigate the proportion of different histological types and CT enhanced imaging features of primary middle mediastinal lesions in order to improve the understanding of these tumors and the accuracy of preoperative diagnosis. Methods: Retrospective analysis was conducted on 84 patients with primary middle mediastinal lesions and clear histological classifications diagnosed and treated at the Cancer Hospital, Chinese Academy of Medical Sciences from January 2012 to December 2022. Clinical, imaging, and pathological data were collected and classified according to tumor histological classifications. CT imaging manifestations such as tumor location, size, morphology, edge, boundary, internal components, enhancement characteristics, and surrounding tissue invasion were evaluated and recorded. Results: The histological types of the primary middle mediastinal lesions from the 84 patients included mesenchymal tumors, anterior intestinal cysts, giant lymph node hyperplasia, substernal goiter, neuroendocrine carcinoma, lymphohematopoietic system tumors, and mesothelioma, accounting for 28.6%, 27.4%, 14.3%, 3.6%, 11.9%, 9.5%, and 4.8%, respectively. Mesenchymal tumors included peripheral nerve sheath tumors, vascular tumors, adipogenic tumors, solitary fibrous tumors, and synovial sarcoma, accounting for 54.2%, 20.8%, 12.5%, 8.3%, and 4.2%, respectively. The above tumors had diverse imaging manifestations and specific imaging features. Mature fat were found in 3 cases of liposarcoma; Calcification was observed in 2 cases of thyroid nodules and 7 cases of giant lymph node hyperplasia; Enhanced scanning showed significant enhancement in 2 cases of solitary fibrous tumors, 3 cases of thyroid nodules, and 11 cases of giant lymph node hyperplasia; Mediastinal large lymph nodes was observed in 6 cases of lymphoma and 3 cases of mesothelioma; High invasiveness was observed in 4 cases of mesothelioma and 9 cases of neuroendocrine carcinoma. Conclusion: Mediastinal tumors have low incidence rate and rich histological types, and their imaging manifestations are diverse. Preoperative differential diagnosis can be made according to their specific imaging characteristics.

#2215 Early molecular and cellular features of aortic remodeling in a mild CKD-MBD model with low bone turnover

Abstract Background and Aims Vascular calcification associated with a maladaptive bone response is common in CKD and is a major cause of mortality (up to 60% depending on CKD stage). Alterations in several intracellular signaling pathways have been reported during the cellular phenotype transition and vascular calcification, however the initial phases of vascular remodeling in early CKD-MBD remain poorly understood. The aim of this study was to investigate the early molecular and cellular features of aortic remodeling prior to calcification in a model of mild CKD-MBD with low bone turnover. Method We induced CKD-MBD by 3/4 nephrectomy (Nx, n = 8) in adult male spontaneously hypertensive rats (SHR) with normal phosphate (Pi) intake (0.6%). Controls were sham-operated Wistar rats (n = 8) and SHR (n = 8). Chronic kidney injury and Pi exchange parameters, serum levels of calciprotein particles (CPPs), intact PTH, intact FGF23, dickkopf-1 and sclerostin, static bone histomorphometry, aortic morphology (H&E, Masson's trichrome, calcein, von Kossa stain), CD31, aSMA, nestin, Col1a1, PDGFR-beta, PiT1, LGR4 and intracellular signaling molecules—phospho-ERK1/2, active (non-phospho) beta-catenin, Hes1, Gli1 were analyzed by IHC with quantitative morphometry and IF after 4 months. Confocal microscopy was performed at the Centre for Collective Use of the Pavlov Institute of Physiology, Russian Academy of Sciences (Zeiss LSM 710). Results The study found that Nx rats with chronic kidney injury comparable to human CKD S2 exhibited lower bone turnover (Fig. 1). The Nx group had higher serum levels of Pi and CPPs vs. control group, but there were no differences in Pi-regulatory factors (Figs 1, 2b). Additionally, the Nx group showed aorta remodeling, which was characterized by an increase in intima thickness, cellularity, and collagen accumulation in the medial layer (Figs 1, 2a). The histological parameters of intimal remodeling and perivascular fibrosis were found to be directly correlated with serum levels of Pi and CPPs. In Nx, there was a decrease in medial αSMA expression accompanied by an increase in phospho-ERK1/2, PiT1, nestin, Gli1, beta-catenin, dickkopf-1, and an decrease in Hes1 immunomorphological staining (Figs 1, 2a, c). αSMA-negative cells demonstrated the expression of osteogenesis-related molecules, such as phospho-ERK1/2 (Fig. 2a, c), and occasionally, RunX2 (Fig. 2a). Phospho-ERK1/2 and PiT1 were found to co-localize in Nx (Fig. 2c). Intimal remodeling was observed, characterized by increased beta-catenin and nestin expression (Fig. 2c). Conclusion In mild CKD-MBD with low bone turnover, early intimal and medial aortic remodeling was associated with higher serum Pi and CPPs levels, and upregulation of PiT1, ERK1/2. In addition, other signals likely related to osteogenesis (RunX2, LGR4) and regulation of vascular progenitor cells (Nestin, Wnt, Notch, Hedgehog) may be implicated.

The KISS Network in 2015–2016: Catalogs and Comparison of the Processing Results with Operational Estimates from the Permanent Network

As part of the international collaboration of several research groups from Russia, France, and Germany, 77 temporary seismic stations were installed in the summer of 2015 for one-year period to conduct a detailed study of the deep structure of the Earth’s crust and upper mantle in the region of the Klyuchevskoi Volcano Group (KGV) in the Kamchatka Peninsula. One of the results of the KISS experiment (Klyuchevskoi Investigation – Seismic Structure of an extraordinary volcanic system) was the final catalog of the joint data from the temporary stations and the permanent network of the Kamchatka Branch of the Geophysical Survey of the Russian Academy of Sciences (KB GS RAS). The catalog comprises 2136 events, including 560 for which the permanent network catalog lacked sufficient data for correct processing. The catalog in .xlsx format and the station bulletin in .isf format are presented in the supplementary material to the paper. A comparative analysis was conducted on the joint solutions of two catalogs: one obtained solely from the data of the KB GS RAS permanent network stations and another from a denser seismic network integrated with KISS stations.

#1464 Alterations of podocytes in focal segmental glomerulosclerosis: a pilot study

Abstract Background and Aims The molecular features of idiopathic podocytopathies remain unexplored, preventing the development of effective therapies to control the mechanisms of disease initiation and progression. This includes idiopathic focal segmental glomerulosclerosis (FSGS), one of the most common forms of immune glomerulopathies (IG) and the cause of renal failure and replacement therapy. In FSGS, podocytes undergo pronounced cytoskeletal alterations. Along with a decrease in Wilms tumor protein (WT1) and α-SMA expression, there is evidence for increased expression of intermediate filament proteins (vimentin, desmin, nestin) and Wnt/β-catenin signaling upregulation in IG. Decrease in WT1 is associated with upregulation in Wnt/β-catenin and p53 to promote podocyte dysfunction and albuminuria. Upregulation desmin increases the mechanical stability of cells, allowing podocytes to undergo morphological changes on the tensile glomerular capillary wall. This pilot study was aimed to evaluate the significance of WT1, β-catenin, and desmin glomerular expression in early primary FSGS. Method We reviewed the histopathological and clinical data of patients with biopsy proven FSGS (N = 14, experimental group), IgA nephropathy (N = 12, positive control), without AKI, infectious diseases, heart failure, respiratory insufficiency and cancer pathology. Renal surgery specimens from patients without IG (N = 12) were studied as negative control. Glomerulosclerosis was assessed as percentage of all glomeruli. Mesangial lesions were measured semi-quantitatively in scores (0-3). Glomerular WT1, β-catenin, and desmin expression was assessed by IHC-P with quantitative morphometry of IHC-staining area (open software QuPath, Orbit Image Analysis). Molecule's co-expression was analyzed by IF-p and confocal microscopy (Zeiss LSM 710; study conducted in the Center for Collective Use “Confocal microscopy” by Pavlov Institute of Physiology Russian Academy of Sciences). Results We examined young and middle-aged patients with CKD stages 1-3 and moderate morphological changes in the glomeruli. Gender, age, eGFR and the percentage of glomeruli with segmental sclerosis were not significantly different between IG groups (all p for interaction >0.05). Patients with FSGS have lower levels of serum albumin (p < 0.001) and higher levels of proteinuria (p < 0.001) as compared to IgAN. Glomeruli from IgAN patients showed more mesangial cell proliferation (p = 0.001), expansion of the mesangial matrix (p = 0.05) and global glomerular sclerosis (p = 0.003) as compared to FSGS. An immunomorphological study showed lower WT1 expression in both IG groups compared to the negative control. In the IgAN, lower WT1 expression was associated with higher BC area (Fig. 1A, D). BC expression did not differ between FSGS and negative control (Fig. 1B,D). In the combined IG group, BC directly correlated with global (r = 0.63, p = 0.001) and segmental (r = 0.63, p = 0.001) glomerular sclerosis. In FSGS, a trend towards to increase in the area of desmin IHC-staining was detected, however, no significant differences between groups were found (Fig. 1C,D). BC and desmin were co-localized with the podocyte marker WT1 (Fig. 2). BC and desmin were also expressed in WT1-negative glomerular cells (Fig. 2). Differential molecular expression patterns in glomeruli have been identified. In IgAN, co-expressing WT1 and BC (orange) was dominate in glomerular cells (Fig. 2A); the proportion of IHC expression of WT1 and BC in glomerular cells was directly correlated (r = 0.63, p = 0.031). In FSGS, WT1+ cells predominantly co-expressed desmin (magenta) but not BC; some glomerular cells were only desmin-positive (Fig. 2B). BC was expressed to a lesser extent compared to IgAN, and in some cells it was co-expressed with desmin (Fig. 2B). Conclusion In FSGS, an increase in desmin expression could be an early feature of pathogenesis before significant decrease in the number of WT1+ podocytes and changes in β-catenin expression. Activation of desmin likely characterizes damage and decrease in the number of functioning podocytes.

Potato productivity on the background of the entomopathogenic fungus <i>Metarhizium robertsii</i> application in production trials

For the first time in Western Siberia (Novosibirsk region), two-year production trials of an experimental prototype of a drug based on the entomopathogenic fungus Metarhizium robertsii have been conducted. Strain P-72 isolated from the dead larvae of Colorado potato beetle Leptinotarsa decemlineata Say from the collection of microorganisms of the Institute of Systematics and Ecology of Animals of the Siberian Branch of the Russian Academy of Sciences was used in this work. Species identification of the fungus was performed based on sequencing of the elongation factor gene region EF1α. The ability of the preparation to significantly increase the parameters of potato yield structure elements throughout the whole vegetation period was shown. A reliable increase in the number of surviving sprouts by 1.4 times, the number of stems and tubers by 1.5 times was found. The growth-stimulating effect of using the preparation based on entomopathogenic fungus was manifested in the increase of plant weight by the flowering phase. The average yield increase for two years amounted to 36.0% in the variant with application of M. robertsii significantly exceeding the productivity of potatoes in the control. The level of profitability of potato production with the use of entomopathogenic fungus M. robertsii increased to 92.0% (with 50.0% in the control) due to the increase in gross yield and improved quality of marketable products. Infestation of new harvest tubers with rhizoctoniose significantly decreased in the variant including treatment with entomopathogenic fungus: the number of tubers with sclerotia decreased 2.8 times compared to the control, which eventually led to an increase in the number of healthy tubers and a significant (2.5 times) decrease in the sclerotial index.

Assessment of the resistance to lodging of spring triticale based on the study of physical and mechanical properties

The results of the study of architectonics and physical and mechanical properties of the stems of selective forms of spring hexaploid triticale of different ecological and geographical origin are presented. Traits of 19 collection and breeding samples from the collections of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) and the Siberian Research Institute of Plant Growing and Selection - branch of the Federal Research Center of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences (SibNIIRS – branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences) served as research objects. Macrometric parameters were studied on 25 representative specimens of each breeding sample when they reached the phase of full ripeness during three years (2021–2023). The resistance of the stem to bending or fracture was determined on an experimental specimen manufactured at the SibNIIRS with a stable platform, bars for fixing the specimen and a slot for placing a dynamometer. The susceptibility of triticale plants to lodging was evaluated by a set of traits: weight of a 10-cm straw of the 2nd internode; stem resistance to breaking determined by the peak reading of an electronic dynamometer; stem tissue density of the 2nd internode. The studied samples differed significantly in these parameters and were characterized by different degrees of the resistance to lodging. Promising breeding specimens that can be used as sources of valuable traits have been identified based on the totality of the strength parameters. Application of the principal component method allowed to identify the marker trait "sum of lengths of the 2nd and 3rd internodes", which was determinant in providing high lodging resistance in the studied samples. A close relationship between the trait of 10-cm straw mass of the 2nd internode and the parameter of the stem resistance to breakage has been established. The conjugation of these characteristics will make it possible to identify the sources of valuable traits and purposefully carry out the selection process of triticale for resistance to lodging without attracting short-stemmed forms.

Analysis of Spatial Clustering of Seismic Events by the DPS Topological Filtering Algorithm: Lake Baikal Region

The paper presents the results of applying the Discrete Perfect Set (DPS) topological filtering algorithm to analyze the spatial clustering of seismic epicenters in the Lake Baikal region. The study utilizes earthquake data recorded by the seismic network of the Baikal Branch of the Geophysical Survey of the Russian Academy of Sciences within the latitude range 48°N, 58°N and longitude range of 99°E, 122°E for the period from 1964 to 2018. Clustering characteristics are obtained for (i) the recording period from 1989 to 2018 with varying parameters of the DPS algorithm and four levels of the minimum energy class KР of seismic events and (ii) six non-overlapping time intervals from 1964 to 2018 and seismic events of energy class KР ≥ 8.6 with fixed parameters of the DPS algorithm. The dynamics of the clustering parameters from 1964 to 2018 may characterise the variability of the seismic regime of the region. Specifically, the decrease in the linear size of the areas of identified epicenter groups from about a thousand km to tens km may indicate a significant change in the seismic regime of the Lake Baikal region at the end of the 1990s and the beginning of the 2000s compared to the period between 1964 and 1997.

#1515 Myocardial remodeling is associated with redistribution of phosphate, PIT1/ERK1/2 up-regulation and signaling pathways alterations in mild CKD model

Abstract Background and Aims Patients with CKD have significantly increased cardiovascular risk, and heart failure is a leading cause of morbidity and mortality in these patients. Myocardial hypertrophy is a hallmark of cardiac remodeling and dysfunction in CKD and is known to be mediated by angiotensin II, catecholamines, hyperphosphatemia, hyperparathyroidism, increase in FGF23, decrease in Klotho and alterations in various signaling pathways including calcineurin, mitogen-activated protein kinase ERK1/2, calmodulin-dependent protein kinase II etc. Less well understood are the mechanisms of fibrosis and intramyocardial arterial remodeling in CKD, which may be related to hyperphosphatemia and decreased renal and systemic Klotho. The aim of this study was to evaluate the molecular and cellular features of myocardial remodeling in experimental mild CKD. Method We induced CKD by 3/4 nephrectomy (Nx) in adult male spontaneously hypertensive rats (SHR) with a follow-up period of 4 months. Animals were fed standard chow containing 0.6% phosphate. Sham-operated Wistar rats and SHR served as controls. We measured serum creatinine, renal interstitial fibrosis area, systolic blood pressure (BP), myocardial mass index (MMI), histological indices of myocardial hypertrophy, interstitial fibrosis and intramyocardial artery (IA) remodeling (H&E, Masson's trichrome, von Kossa stain), myocardial phosphorus content, serum levels of inorganic phosphate (Pi), PTH and FGF23. Myocardial expression of phosphate transporters PiT1, ERK1/2, WNT, BMP, Notch, Hedgehog signaling pathways and LRG4 molecules was evaluated by PCR-RT, IHC and IF (Zeiss LSM 710; study performed at the Centre for Collective Use “Confocal Microscopy” of the Pavlov Institute of Physiology, Russian Academy of Sciences). Results Chronic kidney injury and phosphate regulatory factors did not differ, whereas BP and MMI were higher in sham SHR vs. Wistar rats (Fig. 1). Nx rats showed features of mild chronic kidney injury before the elevation of serum PTH, FGF23 levels (Fig. 1). Myocardial P content and serum Pi were higher in Nx and in the combined SHR group and directly correlated with indices of IA remodeling. Cardiomyocyte diameter, myocardial interstitial and perivascular fibrosis were higher in Nx (Figs 1, 2a) and accompanied with upregulation in Ppp3ca mRNA (calcineurin A, Fig. 2b), Mapk1 mRNA (ERK2, Fig. 2b), phospho-ERK1/2 (Figs 1, 3), PiT1 (Figs 1, 2c), Lgr4 (Fig. 2c) and downregulation of Hes1 mRNA (Fig. 2b). PiT1 and pospho-ERK1/2 were co-expressed in IA media from SHR (Fig. 2c). In Nx, nestin and LGR4 were expressed by IA adventitia and media cells, myocardial interstitial cells, and occasionally co-localized (Fig. 2c). Hes1 expression was detected in IA and capillary endothelial cells and co-localised with Gli1 in IA media; Gli1 was also detected in cardiomyocyte nuclei (Fig. 2c). In SHR, beta-catenin expression in cardiomyocytes was more pronounced in Nx (Fig. 2c). Conclusion Mild CKD is associated with histological and molecular features of cardiomyocyte hypertrophy, myocardial fibrosis and intramyocardial arterial remodeling, which are probably modulated by phosphate redistribution and its tissue retention involving upregulation of PiT1 and ERK1/2, as well as alterations in signals related to progenitor regulation and myocyte hypertrophy.

Xuemei Chen: Original innovation often derives from free exploration

An interview with Xuemei Chen, a distinguished plant biologist, Dean of the School of Life Sciences at Peking University, and a member of the US National Academy of Sciences.

Career adaptability among new oncology nurses: A longitudinal exploration.

This study aims to explore the longitudinal predictive effect of self-awareness on career adaptability in new nurses at a tumor specialty hospital and the mediating mechanisms of work readiness and transition shock. Career adaptability is crucial for the personal development of nurses and also intricately linked to the retention rates among newcomers in oncology nursing. Inadequate career adaptability contributes to higher turnover, which in turn exacerbates the shortage of qualified nursing personnel in this field. There is a pressing need for dedicated research and interventions that support new nurses, especially in specialized areas like oncology, to promote their well-being and career advancement. Comprehending these challenges is essential for devising effective strategies that will retain nursing talent and ensure the sustainability of a robust healthcare workforce. Longitudinal data from four follow-up surveys were collected from 248 new clinical nurses at the National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College. Mediation analyses using R 4.1.2 were conducted to examine the pathways between self-awareness, work readiness, transition shock, and career adaptability. Self-awareness not only directly predicts career adaptability but also influences it through the bidirectional and chained mediating pathways of work readiness and transition shock. These findings equip nursing managers with flexible strategies to foster and enhance nurses' career adaptability, providing targeted support as nurses assimilate into their clinical roles. This not only strengthens workforce stability but also mitigates turnover, ultimately reinforcing the healthcare workforce.

Quality of Life as a Factor in Maintaining the National-State Identity Steadiness

The paper discusses the quality of life capacity as a factor in maintaining the stability of national-state identity in modern Russia. The authors reveal the topic based on the analysis of data from quantitative sociological research for the period 2013–2021 (surveys by the All-Russian Center for the Study of Public Opinion and the Institute of Sociology of the Russian Academy of Sciences). The theoretical basis of the study is the concept of social identity by G. Tajfel and D. Turner. The authors conclude that, by the mid-2010s, a consensus formed among most adult citizens in a Russian society that the quality of life was the key or one of the primary criteria for the national greatness of the country. The model of irrational prestige consumption has become widespread, the extrapolation of which was facilitated by increased access to relevant media content through increased Internet penetration and improved methods of simulation getting status goods and services. The described situation creates risks of destroying the empirical basis for supporting in-group favoritism of Russians and out-group discrimination of communities speaking on behalf of the principal opponents of the Russian Federation in the external domain. Because of this, the quality of life in the medium term gains the role of one of the key factors providing the sustainability of the national-state identity of Russians.

A comparative lattice analysis of $SU(2)$ dark glueball

Abstract We study the mass and scattering cross section of $SU(2)$ glueballs as dark matter candidates using lattice simulations. We employ both naive and improved $SU(2)$ gauge actions in $3+1$ dimensions with several $\beta$ values, and adopt both the traditional Monte Carlo method and the flow-based model based on machine learning techniques to generate lattice configurations. The mass of dark scalar glueball with $J^{PC}=0^{++}$ and the NBS wave function are calculated. 
Using a coupling constant of $\beta=2.2$ as an illustration, we compare the dark glueball mass calculated from the configurations generated from the two methods. While consistent results can be achieved, the two methods demonstrate distinct advantages. Using the Runge-Kutta method, we extract the glueball interaction potential and two-body scattering cross section. From the observational constraints, we obtain the lower bound of the mass of scalar glueball as candidates of dark matter. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Article funded by SCOAP3 and published under licence by Chinese Physical Society and the Institute of High Energy Physics of the Chinese Academy of Science and the Institute of Modern Physics of the Chinese Academy of Sciences and IOP Publishing Ltd.

Satellite Surveillance of the Sea

Prof. Mirosława Ostrowska from the Institute of Oceanology of the Polish Academy of Sciences, Vice President of the Academy, discusses how satellites are being used to study phenomena occurring in seas and oceans.

The type specimens of bees (Hymenoptera, Apoidea) deposited in the Zoological Institute of the Russian Academy of Sciences, St. Petersburg. Contribution VII. Family Andrenidae, genus Andrena Fabricius, 1775, taxa described by A. Osytshnjuk and A. Lebedev

The type specimens for names of bee taxa in the genus Andrena Fabricius, 1775, described by Anna Z. Osytshnjuk and Alexander G. Lebedev which are deposited in the Zoological Institute, Russian Academy of Sciences (St. Petersburg) are reviewed. Primary types of 27 taxa (22 described by A. Osytshnjuk and five described by A. Lebedev) are illustrated and detailed information is provided. A new synonymy is established for Andrena marmora Nurse, 1904=A. gussakovskii Lebedev, 1932, syn. nov. Lectotypes are designated for Andrena azerbaidshanica Lebedev, 1932 and A. cochlearicalcar Lebedev, 1933. A complete list of all taxa of the genus Andrena described by A. Osytshnjuk (88 taxa) and A. Lebedev (11 taxa) is also given, with the locality data of the primary types.