Impedance Spectroscopy Research Articles

Modulation in Spin State of Co3O4 Decorated Fe Single Atom Enables a Superior Rechargeable Zinc-Air Battery Performance.

High-performance bifunctional electrocatalyst for oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) is the keystone for the industrialization of rechargeable zinc-air battery (ZAB). In this work, the modulation in the spin state of Fe single atom on nitrogen doped carbon(Fe1-NC) is devised by Co3O4 (Co3O4@Fe1-NC), and a mediate spin state is recorded. Besides, the d band center of Fe is downshifted associated with the increment in eg filling revealing the weakened interaction with OH* moiety, resulting in a boosted ORR performance. The ORR kinetic current density of Co3O4@Fe1-NC is 2.0- and 5.6 times higher than Fe1-NC and commercial Pt/C, respectively. Moreover, high spin state is found for Co in Co3O4@Fe1-NC contributing to the accelerated surface reconstruction of Co3O4 witnessed by operando Raman and electrochemical impedance spectroscopies. A robust OER activity with overpotential of 352mV at 50mA cm-2 is achieved, decreased by 18 and 60mV by comparison with Co3O4@NC and IrO2. The operando Raman reveals a balanced adsorption of OH* species and its deprotonation leading to robust stability. The ZAB performance of Co3O4@Fe1-NC is 193.2mW cm-2 and maintains for 200h. Furthermore, the all-solid-state ZAB shows a promising battery performance of 163.1mW cm-2.

Kinetics of Oxygen Evolution Reaction in Soluble Lead Flow Batteries

Abstract Kinetics of oxygen evolution reaction (OER) on PbO2 deposited glassy carbon disk electrode in methane sulfonic acid (MSA) is analyzed with cyclic voltammetry and electrochemical impedance spectroscopy, for soluble lead flow battery applications. The effect of concentration of active species (Pb2+), and the electrolyte (MSA) on the OER at the deposited PbO2 layer is investigated. The apparent activation energy of the OER on PbO2, deposited at various overpotential, is obtained from the Arrhenius and Eyring analysis. The apparent activation energy follows a special trend that it increases with overpotential, reaches a maximum and decreases thereafter. This is consistent with the PbO2 specifically deposited from low concentration of MSA (<1M), where the gel layer composed of hydrated PbO(OH)2 is also found. It is observed that low MSA concentration is preferred for limiting the OER at the positive electrode.

Positive impact of the architecture of the oxygen electrode based on LNO and CGO for solid oxide electrochemical cells

The Ruddlesden-Popper phase, strontium- and cobalt-free lanthanum nickelate, La2NiO4+δ (LNO), is a mixed-conducting oxide phase and a promising oxygen electrode for SOCs (solid oxide electrochemical cells), thanks to its high diffusion of oxygen and its surface exchange properties. The electrochemical performance is strongly related to the charge transfer at the triple phase boundaries in the surface path and the excorporation/incorporation reaction in the bulk path. In this context, this study explores a strategy to increase the number of active sites in LNO-based electrodes, by designing nanostructured active functional layers and incorporating gadolinium-doped ceria (CGO). Two architectural scenarios are proposed compared to a pure LNO reference: a uniform distribution of CGO in the volume of the LNO and a continuous compositional gradient, both fabricated for the first time by electrostatic spray deposition (ESD).These designs are investigated based on phase structure, microstructure, elemental chemical composition, and electrochemical properties using SEM-EDS, XRD, and electrochemical impedance spectroscopy. Polarization resistance values are discussed as a function of the distribution of contact points in the electrode volume and LNO crystallization. The results suggest that the presented approach to achieve CGO: LNO-based gradient electrodes allows controlling the localization of the interfaces between the two phases, thereby optimizing charge transfer.

Exploring giant dielectric constant in Dy2CoMnO6: evidence of non-overlapping small polaron hopping

Abstract Perovskites, both natural and synthetic, form a large class of materials are a vast class of materials with significant technological relevance due to their remarkable physical properties, such as superconductivity, magnetoresistance, ionic conductivity, and diverse dielectric behaviors. In this study, the dielectric relaxation and transport properties of the double perovskite Dy2CoMnO6(DCMO) synthesized via high-energy ball milling are investigated. DCMO exhibits a notably large dielectric constant, attributed to a combination of intrinsic and extrinsic mechanisms. Frequency-dependent dielectric studies reveal non-Debye-like behavior, validated by augmented Havriliak-Negami function fitting. Impedance spectroscopy confirms the semiconducting nature of DCMO, showing a negative temperature coefficient of resistance, and identifies two distinct relaxation processes corresponding to grain boundaries and grain interiors thereby highlighting the impact of microstructure and defects. The Cole-Cole plot further supports the non-Debye behavior, while thermally activated relaxation suggests damped charge carrier dynamics at grain boundaries. Conduction analysis using augmented Jonscher's power law reveals non-overlapping small polaron tunneling as the dominant mechanism driving both the dielectric response and transport properties, with DC conductivity suggesting a three-dimensional variable range hopping model. These results provide significant insights into the dielectric and transport properties of DCMO, highlighting its promising potential for advanced electronic applications.

Synthesis and Characterization of Lithium Phosphate (Li3PO4) as a Solid Electrolyte

Due to its high thermal stability, environmental friendliness, and safety, lithium phosphate (Li3PO4) is used as a solid electrolyte in battery applications, but it is usually used with dopants due to its lower ionic conductivity, which is required for ion transport. However, due to its stability and environmentally friendly aspect, lithium phosphate is still a hot topic among suitable energy materials that need further research to improve its electrochemical properties. In the current work, a novel synthesis of lithium phosphate was proposed from the raw materials lithium carbonate (Li2CO3) and trisodium phosphate dodecahydrate (Na3PO4*12H2O) under suitable stoichiometric conditions using the co-precipitation method. In the set of synthesized samples, a single-phase β-Li3PO4 (named LPO-4) with 99.7% purity and 93.49% yield was successfully prepared under appropriate stoichiometric conditions and pH 13 at 90 °C. The average particle size was 10 nm with a large surface area of 9.02 m2g−1. Electrochemical impedance spectroscopy (EIS) of LPO-4 revealed a conductivity of 7.1 × 10−6 S.cm−1 at room temperature and 2.7 × 10−5 S.cm−1 at 80 °C with a low activation energy of 0.38 eV. This performance is attributed to the morphology of the nanotubes and the smaller particle size, which enlarge the reaction interfaces and shorten the diffusion distance of lithium ions. The kinetic and thermodynamic key parameters showed that the β-Li3PO4 exhibits thermal stability in the room temperature range up to 208.8 °C. All these property values indicate a promising application of lithium phosphate as a solid electrolyte in solid-state batteries and a new route for further investigation.

Asymmetric Imidazolium-Based Ionic Liquid Crystal with Enhanced Ionic Conductivity in Low-Temperature Smectic Phases

We report the synthesis and characterization of a novel asymmetric imidazolium-based ionic liquid crystal (ILC) dimer exhibiting stable smectic phases over a wide temperature range, including room temperature. This unique molecular structure, combining two distinct mesogenic cores, reduces packing density, which enhances ion mobility and achieves high ionic conductivity in the smectic phase (0.1 mS cm−1 at 40 °C). Electrochemical impedance spectroscopy (EIS) confirmed improved ionic conductivity at lower temperatures, along with a stable electrochemical window of ±3 V. Application as a solid-state electrolyte in an electrochromic device demonstrated effective switching behavior and reversible redox cycles. These findings suggest that this asymmetric imidazolium-based ILC is a viable candidate for advanced electrochemical applications due to its structural stability and anisotropic ionic pathways.

Pore Wetting and Compaction in Pressure-Driven Distillation: Insights from Impedance Spectroscopy

Pore Wetting and Compaction in Pressure-Driven Distillation: Insights from Impedance Spectroscopy

Ionic Conductivity Studies on Proton Conducting Solid Biopolymer Electrolyte Based on 2-Hydroxyethyl Cellulose (2HEC) Doped with Ammonium Chloride (NH4CL)

Solution casting techniques have been used to create a solid biopolymer electrolyte (SBEs) based on 2-hydroxyethyl cellulose (2HEC) doped with varying amounts of ammonium chloride (NH4Cl) to investigate the potential of NH4Cl in enhancing the ionic conductivity of SBE. Electrical impedance spectroscopy (EIS) was used to measure the impedance of the electrolytes between 50 Hz and 1 MHz in frequency. At room temperature, ionic conductivity was at its highest for 16% weight percent of NH4Cl which was 1.74 x 10-3 Scm-1. The temperature dependence data of 2HEC-NH4Cl SBEs abides Arrhenius behaviour. Complex electrical modulus and complex permittivity were used to assess the dielectric data for the sample at various temperatures.

A novel Dy[formula omitted] doped Zn2V2O7 nanoparticles: Photoluminescence and electrochemical studies for display and supercapacitor applications

A novel series of Dy3+-doped Zn2V2O7 (ZV) nanoparticles (NPs) spanning concentrations from 1 to 9 mol% was synthesized via Menthaspicata leaves extract-mediated solution combustion method. Upon Dy3+ doping, the diffraction pattern matches well with the monoclinic crystal structure with the C2/c(2/m) space group of ZV host matrix. Morphological analysis revealed a transition from irregularly shaped NPs to hexagonal shaped NPs with varying dopant concentration. The crystallite size estimated via Scherrer’s method correlated closely with transmission electron microscopy analysis. Determination of the optical band gap from Tauc’s plot using UV–Visible absorption indicated a tuning from 3.045 to 2.986 eV with increasing dopant concentration. Under 266 nm excitation, the peak at 489 and 586 nm corresponds to 4F9/2→6H15/2 (Magnetic dipole) and 4F9/2→6H13/2 (electric dipole) transition of Dy3+ ion. The dominance of the blue emission (MD) over the yellow emission (ED) of Dy3+ indicates the symmetric nature of the host. The sample exhibits 7 mol % as the optimal doping content. CIE coordinates distinctly falls within the blue region with increasing dopant concentration, with an average color-coordinated temperature of 19902 K, indicative of a cooler appearance. Moreover, cyclic voltammetry analysis provided valuable insights into redox reactions, electrode kinetics, and overall electrochemical behavior, while Electrochemical Impedance Spectroscopy (EIS) offered information on ion transport kinetics. Galvanostatic Charge–Discharge (GCD) analysis revealed super capacitance values ranging from 85.79 F/g to 143.92 F/g at 10 mV/s scan rate, with increasing dopant concentration, highlighting the material’s potential for applications in energy storage and display technology.

Analysis of a fitting model based on polarization characteristics of solid oxide fuel cells

Reliability and long life are the keys to the large-scale commercialization of solid oxide fuel cells (SOFCs), so many studies have focused on how to diagnose SOFC degradation mechanisms. Electrochemical tests have been widely used to study SOFC degradation. The SOFC overpotential can be obtained by measuring the current-voltage (I–V) curves, but these measurements do not show the cause of the SOFC performance attenuation. Electrochemical impedance spectroscopy (EIS) measurements can measure various types of SOFC impedances, but this method is difficult to apply to the high-power stack due to the limited output power of the electrochemical workstation. This paper presents a resistance fitting model based on the I-V measurements for large SOFCs that can determine various electrochemical parameters and resistances by fitting the voltages for different regions of the current densities. The model accuracy is verified by EIS measurements at various current densities with the mean absolute percentage error for each type of resistance given by the fitting model being within 10 % of the EIS measurements which shows that the model can accurately identify the SOFC degradation mechanisms.

Non-destructive AC impedance spectroscopy (ACIS) analysis to characterize the evolution of impact damage in UHPC

Ultra-high performance concrete (UHPC) widely serves as protection material owing to superior impact resistance. Rapid and effective assessment of impact damage level is crucial for ensuring structural safety. The non-destructive AC impedance responds sensitively to impact-induced microstructural changes, providing important information for understanding the damage development mechanism. Therefore, this paper intends to investigate the feasibility of AC impedance spectroscopy (ACIS) for UHPC impact damage detection. UHPC specimens with different types and volume fractions of steel fibers were prepared, subjected to multiple repetitive low-velocity hammer impacts to induce cumulative damage, and the impedance responses were simultaneously tested in the frequency range of 10 Hz-5 MHz. Additionally, the effects of steel fiber distribution characteristics and pull-out mechanism on the conductive path and impact resistance were explained through microstructural analysis and 3D reconstructed model. The results show that ACIS exhibits a strong correlation with impact damage, where the increment of the imaginary part can be used as an effective and sensitive indicator to achieve whole-process damage assessment. Compared to straight steel fibers, hooked-end and corrugated fibers greatly enhance the impact resistance of UHPC but cause a nonlinear response in impedance due to extracted deformations and orifice complexities. These findings demonstrate the potential application of ACIS for detecting impact damage in UHPC and warrant further investigation.

Ultra-thin Atomic Layer Deposited HfO2: Tailoring Dielectric Thickness for Low-Operating Voltage Thin-Film Transistors

To achieve low-power consumption and high performance in TFTs, it is crucial to reduce the thickness of the dielectric gate layer while maintaining a defect-free structure and high dielectric properties. This study explores the effects of varying HfO2 layer thickness on TFT performance. X-ray photoelectron spectroscopy analysis indicates a decrease in oxygen defects as the film thickness increases. The morphological and topological properties of the films were characterized using grazing incidence small and wide-angle X-ray scattering and atomic force microscopy. Impedance spectroscopy was utilized to analyze the dielectric properties, showing that the 10 nm HfO2 film exhibited a low leakage current density of 13 nA cm−2 at 1 V and a permittivity of 5.98. The fabricated ZnO TFT with a 10 nm HfO2 gate dielectric layer operated at an ultra-low voltage of -1.59 V, achieving a high current on/off ratio exceeding 106. These findings highlight the importance of controlling dielectric layer thickness for the development of high-performance TFTs and their potential for low-power microelectronic applications.

In-vitro sensing of traumatic brain tissue by electrochemical impedance for diagnosis and therapeutic

Traumatic brain injury (TBI) induces neurogenerative disorders affecting severely daily human activities and early diagnosis is a critical requirement for prevention and cure. Here, we induced TBI formation in the Zebra fish, a model organism, by both mechanical (ultrasonic cleaner generated convulsive shock, UGCS) and chemical (pentylenetetrazol, PTZ) methods. The TBI induced cellular and neuronal changes are monitored by measuring the activities of the indicator biomarkers viz., superoxide anion (O2−) and glutamate by electrochemical techniques. For this, the α-lipoic acid (α-LA, LA) functionalized gold-silver (LA-Au/Ag) is used as an electrochemical sensor to diagnose the presence of these markers in physiological phosphate buffer saline (PBS, pH 7.4), 0.1 M KCl solutions and in TBI tissues. While the oxidation of glutamate is observed in the potential window 0.2–0.5 V, the metal mediated oxidation of O2− is observed at the potential window 0.6–1.0 V. The sensor showed good linear ranges for O2− (from 4 to 48 μM with the LOD of 4 μM for the O2− detection) and glutamate (from 20 to 130 μM with the LOD 19 μM). The TBI tissue modified electrode showed lower resistance than the normal brain tissue ((NBT), as control) due to the presence of higher amount of O2− and occurrence of Fenton’s and Heber-Weise’s reactions in the presence of [Fe(CN)6]3−/4−. For theragnostic application, the LA-Au/Ag nanoparticles is delivered into the UGCS and PTZ treated Zebrafish and electrochemical signal changes are monitored by cyclic voltammetry and impedance spectroscopy. Electrochemical data further corroborated with the activities of superoxide dismutase (SOD), Catalase (CAT) and lipid peroxidase (MDA) in parallel. The developed method of electrochemical sensing of TBI may provide alternative for the early TBI diagnosis and therapeutics for the prevention of TBI.

Synthesis and ionic conductivity of Na1+2xMxZr2-x(PO4)3 (M – Mg, Mn) NASICON-type ceramic materials

Ceramics of the Na1+2xMxZr2-x(PO4)3 (M – Mg, Mn) composition were synthesized by the co-precipitation technique with subsequent annealing. The samples were characterized using X-ray diffraction with the Rietveld refinement, scanning electron microscopy, and impedance spectroscopy. The studied phosphates belong to the NASICON type structure. The solid solutions (0 ≤ x ≤ 1.0) were shown to be formed in the investigated systems. To study the effect of the chemical composition and sintering additive on conductivity, two series of ceramics were prepared for each phosphate: with and without ZnO (2 wt.%) additive. The ionic conductivity of the studied phosphates followed the Arrhenius law and passed through a maximum with x growth. It was shown that the addition of zinc oxide also leads to partial substitution of zirconium in the lattice and an increase in ionic conductivity. The highest conductivity was achieved for the Na2.6Mg0.8Zr1.2(PO4)3 ceramic with 2 wt.% ZnO additive (6.8∙10-3 S∙cm-1 at 673 К).

A new organic–inorganic chloride (H3N–(CH2)6–NH3)[SnCl6]: Crystal structure, thermal analysis, vibrational study, and electrical properties

In this work, a new Organic-Inorganic chloride (H3N–(CH2)6-NH3)[SnCl6] (1) has been synthesized and characterized by single crystal X-ray diffraction (XRD), IR, Raman and impedance spectroscopies. The crystallographic study displays that the title compound crystallizes in the triclinic system with the P−1 space group. The vibrational study (IR, Raman) at room temperature confirmed the existence of the organic and inorganic functional groups. Differential scanning calorimetry (DSC) analysis reveals the existence of three reversible phase transitions at T1 = 345/325 K, T2 = 483/443 K, and T3 = 496/465 K (Heating/Cooling). Furthermore, the conductivity analysis and the dielectric properties confirm the presence of these phase transitions. AC-conductivity measurement of (1) has been investigated using complex impedance spectroscopy in frequency and temperature range 10 Hz–5 MHz and 313–523 K, respectively. The study of Nyquist plots showed the contribution of grains and grain boundaries in the electrical study, confirming the existence of a non-Debye type relaxation. The AC conductivity shows that the material has the potential of impedance sensors.

Eco-friendly corrosion inhibition of steel using phenolic compounds from Cynara syriaca

This study evaluates the corrosion inhibition performance of the n-butanol (n-BuOH) fraction derived from Cynara syriaca on mild steel in acidic environments. The n-BuOH fraction achieved up to 94 % inhibition at 500 ppm, as assessed through gravimetric analysis, potentiodynamic polarization (PDP), and electrochemical impedance spectroscopy (EIS). Key phenolic compounds, including kaempferol, naringenin, myricetin, chlorogenic acid, and quercetin, were identified by UPLC-MS/MS. These compounds form stable flavonoid-metal complexes, as confirmed by UV–Vis spectroscopy. FT-IR and SEM-EDS analyses revealed the formation of protective coatings and interactions with the steel surface. Polarization curves indicate that the n-BuOH fraction acts as a mixed-type inhibitor, predominantly affecting the anodic reaction. The inhibition efficiency decreased from 93.89 % to 73.43 % with increasing temperatures. Thermodynamic analysis showed an increase in activation energy (Ea = 58.20 kJ/ mol) and a positive ΔH value of 56.17 kJ/mol, which can be attributed to the increased thickness of the double layer, enhancing the activation energy of the corrosion process. A Gibbs free energy (ΔG0ads) value of −25.48 kJ/mol confirms that the adsorption process is spontaneous and involves both physisorption and chemisorption. pKa analysis identified specific adsorption sites. This research underscores the potential of the n-BuOH fraction as a novel, eco-friendly corrosion inhibitor, offering valuable insights for sustainable corrosion control strategies.

Synergistic effects of gadolinium oxide into the matrix of zeolitic imidazolate frameworks (ZIFs) for supercapacitor applications

In this study, we synthesized ZIF-8, ZIF-67, Gd₀.₀₁/ZIF-8, & Gd₀.₀₁/ZIF-67 electrodes using the direct combination technique. Both electrodes are being used for energy storage devices. We extensively evaluated these nanocomposites using a variety of electrochemical methods, including retention analysis, galvanostatic charge-discharge (GCD), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). ZIF-8 exhibits a wide band gap of approximately 2.58 eV, suggesting its main absorption is of UV light. The peaks in the absorbance spectrum vary slightly in wavelength due to the presence of different metal centers. Peaks between 1400-1600 cm⁻1 indicate C–N stretching vibrations from the imidazolate linkers. Peaks between 1600-1700 cm⁻1 are associated with C=C stretching vibrations. ZIF-8 exhibits well-defined peaks in its XRD pattern, indicating a high level of crystallinity. Peaks can be observed at specific 2θ values, typically at 22.09°, 26.62°, 38.49°, and 47.27°, which correspond to the (110), (200), (211), and (220) planes. The calculated specific capacitances for ZIF-8 and ZIF-67 are 223.95 and 255.20 F/g. For Gd0.01/ZIF-8, & Gd0.01/ZIF-67 specific capacitances are 575.00 and 587.50 F/g. The ZIF-8, ZIF-67, Gd0.01/ZIF-8, & Gd0.01/ZIF-67 electrodes exhibited specific capacitances of (78.30, 192.59, 342.57, and 1164) F/g at current densities of 1 A/g from the GCD calculation. The retention plot of ZIF-8, ZIF-67, Gd0.01/ZIF-8, & Gd0.01/ZIF-67 electrode shows efficiency of 71%, 71%, 105% and 75%, respectively, indicating their suitability for supercapacitor applications.

Synthesis, Structure, and Electrochemical Performance of Bi-induced Stabilization of MnO2 Cathodes for Use in Highly Acidic Aqueous Electrolytes (pH

MnO2 cathode materials are widely studied in alkaline and neutral aqueous electrolytes. In these mediums, the MnO2 cathode shows suboptimal performance limited by dissolution and electrochemically inactive compound formation, leading to capacity fading. This study explores the enhancement of MnO2 cathode performance through Bi3+ ion doping (0, 1, 2.5, 5, and 10mol%) in a highly acidic electrolyte (pH < 2). By incorporating up to 10mol% Bi ions into the MnO2 structure, we significantly improved specific capacity and capacity retention stability. Energy-dispersive X-ray spectroscopy (EDX) analysis revealed a uniform dispersion of Bi3+ ions throughout the MnO2 cathode after electrochemical cycling, contributing to performance enhancements. X-ray photoelectron spectroscopy (XPS) results indicated that Bi3+ ion concentration from 1 to 10mol% stabilises Mn3+ within the MnO2 lattice. Also, Bi3+ ion doping promotes the formation of a 2×2 tunnel structured α-MnO2 phase. Electrochemical impedance spectroscopy results demonstrated a reduction in double-layer and overall bulk capacitance. These findings suggest that Bi3+ ion doping effectively enhances MnO2 electrochemical performance and could enhance its use in aqueous metal-ion batteries.

Influence of the mechanical activation process on the corrosion of Ti2⁠AlN MAX phase obtained by hot pressing

The Ti2AlN MAX phase is known for its unique blend of metallic and ceramic properties, including high electrical and thermal conductivity and excellent oxidation and corrosion resistance. This study focuses on the influence of the mechanical activation process on the electrochemical properties of Ti2AlN in a saline environment. High-purity powders of AlN and Ti were mechanically activated or mixed and sintered to synthesize the Ti2AlN MAX phase. The electrochemical behavior was assessed through potentiodynamic curves and electrochemical impedance spectroscopy, while surface characteristics were analyzed using SEM-EDS and XPS post-exposure to a 3.5% NaCl solution. Results reveal that Ti2AlN forms protective Al2O3 and TiO2 layers, enhancing its corrosion resistance. Mechanically activated samples (MAP) displayed different corrosion behavior than non-activated samples (NMAP), with MAP showing reduced corrosion resistance. This discrepancy is attributed to differences in oxide formation and microstructural changes due to mechanical activation. This research not only elucidates the corrosion mechanisms of MAX phases in chloride-rich environments but also analyzes the effect of mechanical processing on their properties, contributing to developing Ti2AlN applications where high durability and resistance are critical.

Synthesis and nanoparticle formation of pyrazine, benzodithiophene (BDT) and fluorene containing conjugated polymer (P-PBF) and its biosensing performance toward catechol

The development of laccase-based biosensors, in particular, have attracted much interest due to their ability to detect highly toxic molecules in the environment. This article reports a novel biosensor for catechol detection that can be used to determine toxic molecules in the environment. For this purpose, a novel conjugated polymer (P-PBF) and conjugated polymer nanoparticles (P-PBFNPs) were synthesized and characterized, respectively. To our knowledge, research has yet to be done on conjugated polymer nanoparticle-based electrochemical biosensing platforms for catechol determination. The biosensor comprises a GE/P-PBF NPs/Lac electrode, constructed with P-PBF NPs deposited onto a bare graphite electrode followed by laccase (Lac) enzyme immobilization. While electrochemical characterization was performed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), surface characterization was performed by field emission scanning electron microscopy (FE-SEM). The biosensor exhibited high sensitivity (1.229 μA/μM.cm2), a good linear range (0.5–25 μM) and a low limit of detection (LOD) (0.49 μM). Moreover, the developed biosensor exhibited high selectivity in the presence of interfering analytes without any memory effects. The potential of the GE/P-PBF NPs/Lac electrode in tap water, green tea, synthetic urine and serum samples indicates the inspiring biosensing applications for monitoring environmental pollution.