Abundant Bat Species Research Articles

Frugivorous bat (Chiroptera: Phyllostomidae) community structure and trophic relations in Atlantic Forest fragments

We evaluated the structure of a community of frugivorous bats using composition and abundance patterns, niche amplitude and food overlap of these animals in four Atlantic Forest fragments, each one exposed to different conservation realities. For twelve months, we captured six bat species and found the seeds of 13 species of pioneering plants in 158 fecal samples. The most abundant bat species were Artibeus planirostris (25.4%), Artibeus lituratus (24.1%) and Carollia perspicillata (23.9%). Only one fragment (Fazenda Unida), the most conserved area, exhibited a significantly different composition and abundance of species. We found low trophic niche amplitude values (<0.60), associated to high food overlaps. Our results suggest that bats can adjust their foraging strategy to deal with food availability variations. By favoring pioneering plant species, the fragmentation process noted of the studied areas creates an attractive environment for bats more tolerant to this type of disturbance. The sampled areas represent important secondary forest remnants in southern Brazil that require attention to avoid an even greater loss of bat diversity.

Parasitic interactions between bats (Mammalia: Chiroptera) and flies (Insecta: Diptera) in the intersection area of temperate and tropical climates in Brazil

ABSTRACT Bats and ectoparasites, Streblidae and Nycteribiidae, are good models to study ecological interactions due to their specificity. Knowledge about the interactions between these groups is heterogeneous and fragmented throughout Brazilian territory, mainly in the southern region – influenced by the temperate climate with bat fauna presenting elements from both tropical and temperate areas. We evaluated the interaction of parasites and bats in an Atlantic Forest area, southern Brazil. We captured bats and their ectoparasites monthly in 10 campaigns using mist nets. To describe the interactions, we used prevalence and average intensity of infestation, the same indexes we used to compare host sexes. We captured 494 bats, from which 151 had ectoparasites. Artibeus lituratus and Sturnira lilium were the most abundant bat species. We captured 303 ectoparasites, from which Metelasmus wenzeli is a new record to the state of Santa Catarina. Paratrichobius longicrus and Megistopoda proxima were the most abundant ectoparasite species, probably due to the greatest abundances of the host species of bats A. lituratus and S. lilium in this study. Most of the interactions we found are primary associations, which reinforces the specific parasite–host relationship. The data we obtained here are the first for the state of Santa Catarina.

Pemetaan Spesies Kelelawar Berdasarkan Pola Pemilihan Sarang di Gua Gale-gale Bangkang dan Gua Raksasa Tanjung Ringgit

This study aims to determine the pattern of nest selection in bat species inhabiting the Bangkang Gale-gale Cave in Prabu Village, Pujut District, Central Lombok Regency, and Tanjung Ringgit Giant Cave in Sekaroh Village, Jerowaru District, East Lombok Regency. Nests are an important component of bat life. Most types of bats live in colonies in nesting and foraging for food. This research method is descriptive exploratory by mapping cave zones, making squares, analyzing the richness and abundance of bat species at each bat nesting site. The results showed that the nest selection pattern in Gale-gale Cave, namely: Rhinolopus acuminatus, Rhinopoma microphylum, and Rhinolopus simplex were in the same group in zone / zone I. Whereas Chaerephon plicata could be found inhabiting zone / zone II, and Hipposideros ater. saevus inhabit zone / zone III. The pattern of nest selection in the Giant Cave Tanjung Ringgit, Macroglossus minimus and Eonycteris spelaea was found in both zone zone II and zone III caves. Hipposideros ater saevus and Hipposideros diadema are in the same group, namely on the border between zone II and zone III, namely the left and right sides of zone III, or not far from the water source in the cave ± 2 m above the water source, and Rosettusa amplxicaudatus inhabit zone / zone III Giant Cave Tanjung Ringgit.

Parasitization of bats by bat flies (Streblidae) in fragmented habitats

AbstractParasites represent a large fraction of the world's biodiversity. They control host population sizes and contribute to ecosystem functioning. However, surveys on species diversity rarely include parasitic species. Bats often present traits favoring parasite diversity, such as large home ranges, long life spans, and large colonies. The most conspicuous bat parasites are the highly host‐specific, blood‐sucking bat flies (Diptera: Streblidae, Nycteribiidae). Recent studies have found a direct effect of habitat alteration on the abundance of bat species. We expected, therefore, that changes in the host community in response to anthropogenic habitat modification will also result in changes in the associated parasite community. We captured bats in three different habitats in Central Panama between 2013 and 2015. We recorded information on prevalence and intensity of bat fly parasitization of the seven most commonly captured bat species. Prevalence and intensity were both significantly influenced by roost type, abundance, and host sex and age. We found that habitat variables and matrix type significantly influenced the prevalence and intensity of parasitization, while the direction of the responses was host species‐ and parasite species‐specific. In general, roosting conditions and behavior of host bats appear to be fundamental in explaining changes in prevalence and intensity of parasitization between different habitat types, as bat flies are bound to the roost during their reproductive cycle. Habitat alterations affect next to the host community composition also the availability of possible roost structures as well as microclimatic conditions, which all three reflect in parasitization.Abstract in Spanish is available with online material.

Rapid molecular species identification of indigenous bats from Germany for surveillance purposes

Chiroptera form the second largest order of mammals and compromise >1200 species, of which only 51 species are abundant in Europe. As bats are important hosts involved in the emergence and spread of zoonotic infections, it is becoming more important to discriminate the different species of bats involved in the maintenance of causative agents. However, traditional taxonomic methods rely on morphological features and are challenging as they require long-lasting experience of an investigator and sometimes fail if the specimen is of poor condition. On the other hand, barcoding requires sequencing and is time consuming. Therefore, a versatile genetic approach for rapid species identification would be valuable.In this study, two mitochondrial loci, cytochrome b (cyt b) and cytochrome c oxidase subunit I (COI) were selected for the development of two multiplex qPCRs for differentiating four very abundant bat species in Germany using DNA extracted from the patagium or organ pools. Verification of the developed assays using a set of 1000 individual bat samples belonging to 20 different European species clearly showed that the multiplex qPCRs were able to determine the four most abundant species in this collection by a COI based qPCR. All other bat species which could not be covered by this approach could be easily identified by sequencing of the amplicon generated by broad-range qPCRs for cyt B and COI, respectively. Moreover, the double-check approach with cyt B and COI makes the identification of bats into species more reliable.The new multiplex PCRs allow a fast and easy genotyping of German bats and could be useful for screening approaches.

Plecotus macrobullaris (Chiroptera: Vespertilionidae)

Plecotus macrobullaris (Kuzjakin, 1965) is a medium-sized chiropteran commonly called the alpine long-eared bat, and 1 of the 11 Palearctic species recognized in the genus Plecotus. It has the characteristic long ears of plecotine bats, and externally its most conspicuous diagnostic characteristic is the inverted triangular-shaped pad most individuals exhibit in the chin. It is distributed across the mountain environments in the southwestern and central regions of the Palearctic. It is the most abundant bat species in European alpine environments, although it can be also found at lower elevations. It roosts on both natural (rock crevices, caves, and scree deposits) and artificial shelters (buildings), and it mainly feeds on moths. P. macrobullaris is currently listed as “Least Concern” by the International Union for Conservation of Nature and Natural Resources, although actual population sizes and demographic trends are unknown.

Bats of the medieval Jeroným mine (western Bohemia, Czech Republic): results of a long-term monitoring in the hibernation and swarming periods (Chiroptera)

The medieval mine of Jeroným (Jerome) is one of the most important bat localities known in western Bohemia. Ten bat species have been detected there, including Myotis emarginatus which is very rare in the western part of the Czech Republic. Numbers of wintering bats have been monitored since 1995 and show an upward trend there, even after the opening of a part of the mine to the public during summer (since 2014). The highest numbers of wintering bats correspond to unusually cold winters in 2017 (347 inds.) and 2013 (156 inds.). Mistnetting during the swarming period has been carried out annually since 2009. Numbers of netted bats have been decreasing, probably due to the existence of a new visitor centre built near the entrance to the underground. Species compositions recorded during the hibernation and swarming periods are very similar, but proportions of particular species are different. Myotis daubentonii, M. myotis and M. nattereri were the most abundant bat species during hibernation, while Myotis nattereri, M. daubentonii and Plecotus auritus during the swarming period. Swarming numbers of Myotis mystacinus and M. brandtii have been growing in the last several years. Myotis bechsteinii was detected repeatedly, while Eptesicus nilssonii and Myotis emarginatus (wintering) and Nyctalus noctula (swarming) only once each. The swarming activity of bats was highest 2.5–5.5 hours after sunset. In total, 436 bats were ringed, about 6% of them were recaptured at the site. Some of the ringed bats were recorded at other localities, Myotis myotis at distances up to 39 km, and M. nattereri up to 21 km.

Season-modulated responses of Neotropical bats to forest fragmentation.

Seasonality causes fluctuations in resource availability, affecting the presence and abundance of animal species. The impacts of these oscillations on wildlife populations can be exacerbated by habitat fragmentation. We assessed differences in bat species abundance between the wet and dry season in a fragmented landscape in the Central Amazon characterized by primary forest fragments embedded in a secondary forest matrix. We also evaluated whether the relative importance of local vegetation structure versus landscape characteristics (composition and configuration) in shaping bat abundance patterns varied between seasons. Our working hypotheses were that abundance responses are species as well as season specific, and that in the wet season, local vegetation structure is a stronger determinant of bat abundance than landscape‐scale attributes. Generalized linear mixed‐effects models in combination with hierarchical partitioning revealed that relationships between species abundances and local vegetation structure and landscape characteristics were both season specific and scale dependent. Overall, landscape characteristics were more important than local vegetation characteristics, suggesting that landscape structure is likely to play an even more important role in landscapes with higher fragment‐matrix contrast. Responses varied between frugivores and animalivores. In the dry season, frugivores responded more to compositional metrics, whereas during the wet season, local and configurational metrics were more important. Animalivores showed similar patterns in both seasons, responding to the same group of metrics in both seasons. Differences in responses likely reflect seasonal differences in the phenology of flowering and fruiting between primary and secondary forests, which affected the foraging behavior and habitat use of bats. Management actions should encompass multiscale approaches to account for the idiosyncratic responses of species to seasonal variation in resource abundance and consequently to local and landscape scale attributes.

The Recently Discovered Bokeloh Bat Lyssavirus: Insights Into Its Genetic Heterogeneity and Spatial Distribution in Europe and the Population Genetics of Its Primary Host.

In 2010, a novel lyssavirus named Bokeloh bat lyssavirus (BBLV) was isolated from a Natterer's bat (Myotis nattereri) in Germany. Two further viruses were isolated in the same country and in France in recent years, all from the same bat species and all found in moribund or dead bats. Here we report the description and the full-length genome sequence of five additional BBLV isolates from Germany (n=4) and France (n=1). Interestingly, all of them were isolated from the Natterer's bat, except one from Germany, which was found in a common Pipistrelle bat (Pipistrellus pipistrellus), a widespread and abundant bat species in Europe. The latter represents the first case of transmission of BBLV to another bat species. Phylogenetic analysis clearly demonstrated the presence of two different lineages among this lyssavirus species: lineages A and B. The spatial distribution of these two lineages remains puzzling, as both of them comprised isolates from France and Germany; although clustering of isolates was observed on a regional scale, especially in Germany. Phylogenetic analysis based on the mitochondrial cytochrome b (CYTB) gene from positive Natterer's bat did not suggest a circulation of the respective BBLV sublineages in specific Natterer's bat subspecies, as all of them were shown to belong to the M. nattereri sensu stricto clade/subspecies and were closely related (German and French positive bats). At the bat host level, we demonstrated that the distribution of BBLV at the late stage of the disease seems large and massive, as viral RNA was detected in many different organs.

Habitat diversity promotes bat activity in a vineyard landscape

Intensification of agricultural production has greatly limited the capacity of agricultural land to support other species. Maintaining landscape heterogeneity in and around agricultural landscapes can help conserve biodiversity and potentially natural pest control. Whereas bats (order: Chiroptera) are highly valued as natural predators of agricultural pests, little is known about the distribution and abundance of bat species across different types of agricultural landscapes. The objective of our study was to assess how local remnant habitat and surrounding natural areas influence bat activity levels within a vineyard landscape. To accomplish this, we conducted acoustic surveys at 21 vineyards within the North Coast wine-grape growing region of California. Using generalized linear mixed-models, we assessed the influence of local remnant habitat and surrounding natural areas to predict overall and species-specific activity patterns. A total of 14,613 bat passes were recorded, of which 80% were identified by a random forest classifier as either Tadarida brasiliensis the Brazilian free-tailed bat (25%), Myotis yumanensis the Yuma Myotis (24%), or Eptesicus fuscus the big brown bat (23%). The results of our models indicate that total bat activity, and the activity of each of the common bat species, was higher adjacent to remnant vegetation along the edges of vineyards as compared to within the vineyard, but that the effect of landscape-scale characteristics on bat activity was weak. This suggests that natural trees and shrubs should be conserved and restored throughout the vineyard landscape to enhance bat abundance for a win-win agricultural production and conservation solution.

Ticks infesting bats (Mammalia: Chiroptera) in the Brazilian Pantanal

Ticks associated with bats have been poorly documented in the Neotropical Zoogeographical Region. In this study, a total of 1028 bats were sampled for tick infestations in the southern portion of the Brazilian Pantanal. A total of 368 ticks, morphologically identified as Ornithodoros hasei (n = 364) and O. mimon (n = 4), were collected from the following bat species: Artibeus planirostris, Platyrrhinus lineatus, Phyllostomus hastatus, Mimon crenulatum and Noctilio albiventris. Morphological identification of O. hasei was confirmed by molecular analysis. Regarding the most abundant bat species, only 40 (6.2%) out of 650 A. planirostris were infested by O. hasei, with a mean intensity of 7.2 ticks per infested bat, or a mean abundance of 0.44 ticks per sampled bat. Noteworthy, one single P. hastatus was infested by 55 O. hasei larvae, in contrast to the 2.5-7.2 range of mean intensity values for the whole study. As a complement to the present study, a total of 8 museum bat specimens (6 Noctilio albiventris and 2 N. leporinus), collected in the northern region of Pantanal, were examined for tick infestations. These bats contained 176 ticks, which were all morphologically identified as O. hasei larvae. Mean intensity of infestation was 22, with a range of 1-46 ticks per infested bat. Our results suggest that A. planirostris might play an important role in the natural life cycle of O. hasei in the Pantanal.

Bats like vintage: managing exotic eucalypt plantations for bat conservation in a Mediterranean landscape

AbstractThe transformation of native habitats into forest plantations for industrial purposes frequently has negative consequences for biodiversity. We evaluated the impact of eucalypt plantations on native bats in a Mediterranean area, taking Portugal as a case study. We compared the overall bat activity, species richness and Kuhl's bat Pipistrellus kuhlii (the most abundant bat species in the area) activity between eucalypt plantations and native montado habitat, and examined the influence of stand, landscape and survey variables within plantations on the response variables. A set of 11 plantation stands, three landscape and two survey variables were employed as predictor variables using a zero‐inflated Poisson generalized linear mixed model. Hawking and generalist bats of the genus Pipistrellus were the most frequently detected species. Bat activity, species richness and P. kuhlii activity were higher in native montado than in any of the eucalypt stands. Mature eucalypt plantations showed the highest bat activity, while clear‐cut areas showed the lowest. Generally, within eucalypt stands, complex high‐level vegetation structure, from the ground level up to 3 m high, and proximity to water points were associated with higher levels of bat and P. kuhlii activity and species richness. The results suggest that in order to promote bat diversity and activity in exotic eucalypt plantations in the Mediterranean region, it is important to provide a high density of water points, maintain plots of mature plantations and promote understorey clutter.

Disease and community structure: white‐nose syndrome alters spatial and temporal niche partitioning in sympatric bat species

AbstractAimEmerging infectious diseases present a major perturbation with apparent direct effects such as reduced population density, extirpation and/or extinction. Comparatively less is known about the potential indirect effects of disease that likely alter community structure and larger ecosystem function. Since 2006, white‐nose syndrome (WNS) has resulted in the loss of over 6 million hibernating bats in eastern North America. Considerable evidence exists concerning niche partitioning in sympatric bat species in this region, and the unprecedented, rapid decline in multiple species following WNS may provide an opportunity to observe a dramatic restructuring of the bat community.LocationWe conducted our study at Fort Drum Army Installation in Jefferson and Lewis counties, New York, USA, where WNS first impacted extant bat species in winter 2007–2008.MethodsAcoustical monitoring during 2003–2011 allowed us to test the hypothesis that spatial and temporal niche partitioning by bats was relaxed post‐WNS.ResultsWe detected nine bat species pre‐ and post‐WNS. Activity for most bat species declined post‐WNS. Dramatic post‐WNS declines in activity of little brown bat (Myotis lucifugus, MYLU), formerly the most abundant bat species in the region, were associated with complex, often species‐specific responses by other species that generally favoured increased spatial and temporal overlap with MYLU.Main conclusionsIn addition to the obvious direct effects of disease on bat populations and activity levels, our results provide evidence that disease can have cascading indirect effects on community structure. Recent occurrence of WNS in North America, combined with multiple existing stressors, is resulting in dramatic shifts in temporal and spatial niche partitioning within bat communities. These changes might influence long‐term population viability of some bat species as well as broader scale ecosystem structure and function.

Bats (Mammalia: Chiroptera) in urban fragments of Maringá, Paraná, Brazil

Bat assemblages in two urban fragments of Maringá city, north of the state of Paraná, southern Brazil, were inventoried. These fragments, Parque do Ingá (48 ha) and Parque Florestal dos Pioneiros (59 ha), are located in a subtropical region covered by semideciduous Atlantic forest. Bats were sampled with mist-nets from August 2006 to July 2007, over 24 nights (12 hours a night), adding up to a total of 30,240 h.m2 of net effort. A total of 839 individuals were captured belonging to 10 species and four families. Artibeus lituratus was the most abundant species (66% of the sample). The observed richness represents 22% of the bat species recorded for the state, 24% of the bat species occurring in the seasonal semideciduous forest of Paraná, 26% of the species previously recorded in urban environments in Brazil, and 83.4% of the estimated richness by Jackknife 1 (n= 12 species). Studies that provide data on richness and abundance of bat species in urban fragments are becoming increasingly important, but are still poorly available in Brazil. This habitat is especially interesting because anthropogenic pressure can be harmful to bat assemblages, reducing their diversity.

Distribution, Abundance and Roosts of the Fruit BatArtibeus fraterculus(Chiroptera: Phyllostomidae)

Where does a species live? How common is it? Where does it spend its inactive periods? These are basic questions about the biology of a species, which bring key information for application in conservation and management. Unfortunately, this information is available for only a minimum fraction of all animal species. Using 1) ecological niche modeling with maximum entropy (Maxent), 2) relative abundance estimates using museum records, and 3) field surveys of roosting sites, we report the fraternal fruit-eating bat, Artibeus fraterculus, as having a distribution limited to the Tumbesian ecoregion in Ecuador and west central Peru, being the relatively most abundant bat species throughout its range, with healthy populations which are primarily sustained by cultivated and introduced plants, and using human-made constructions as roost sites. Additionally, we described a large congregation of individuals of this species in a single roost, representing the largest colony reported for the genus Artibeus. These results may indicate resilience of A. fraterculus to human disturbance.

Enriching agroecosystems with fruit-producing tree species favors the abundance and richness of frugivorous and nectarivorous bats in Veracruz, Mexico

We evaluated how the abundance and richness of frugivorous and nectarivorous bat species differs among three types of common agroecosystems (diversified coffee plantations, simple coffee plantations and pastures) in Veracruz, Mexico, that represent a gradient of structural and floristic complexity. Using mixed effects models we demonstrated that both the richness and the total abundance of bats was higher in the diversified coffee plantations. We detected similar patterns on comparing the abundance of the four most abundant bat species. Neither season nor the season-agroecosystem interaction had any effect on the comparisons made. Using multiple regressions we found that the richness of plants that are useful to both people and bats had the most explanatory power for the richness and total abundance of frugivorous and nectarivorous bats, as well as for the abundance of Carollia sowelli, Glossophaga soricina and Sturnira spp. Our results indicate that agroecosystems value for conservation of fruit and nectar-eating bats increases as the fruit-bearing trees increases. For the effective conservation of these guilds of bats in tropical agroecosystems, a strategy of diversification with fruit-bearing species is highly recommended; such a strategy would benefit both agricultural producers and wildlife.

Abundance responses of frugivorous bats (Stenodermatinae) to coffee cultivation and selective logging practices in mountainous central Veracruz, Mexico

Shifting and permanent cultivation, selective logging, cattle production and coffee plantations are among the most important factors in montane cloud forest conversion and disturbance. Although shaded-coffee plantations can contribute to the preservation of local species richness, abundance of organisms could be determined by habitat resource availability in agricultural landscapes. We compared abundance of Sturnira and Artibeus bats (Phyllostomidae, Stenodermatinae), in shade coffee plantations and disturbed cloud forest fragments, which represent habitats with different chiropterochorous plant density. We also investigated the relationship between bat species abundance and food plant richness, abundance and diversity. We captured 956 bats, 76% in cloud forest fragments and 24% in shaded coffee plantations. Abundance of Sturnira spp. (small bats) was greater in cloud forest than in coffee plantations, but Artibeus spp. (large bats) abundance was similar in both habitats. Chiropterochorous plant abundance was positively related with bat abundance for Sturnira spp., while chiropterochorous plant richness and diversity were negatively related for Artibeus spp. This suggests that frugivorous bats with different morphological and ecological characteristics respond differentially to anthropogenic activities. For landscape management purposes, the maintenance and augmentation of diverse food resources, for frugivorous bats with different foraging requirements in coffee plantations, will benefit the resilience of bats to modification of their natural habitat.

Effects of different secondary vegetation types on bat community composition in Central Amazonia, Brazil

AbstractThe process of secondary succession on degraded lands in the Amazon depends on their land‐use histories. In this scenario, little is known about how animal communities respond to different types of secondary vegetation in the region. We examined the effects of abandoned cattle pasture, Vismia‐ and Cecropia‐dominated regrowth on the abundance of bat species and community composition in the Central Amazon, Brazil, based on 11 netting sites and on landscape characteristics. We captured 1444 bats, representing 26 species and two families (Phyllostomidae and Mormoopidae). Among the six most‐captured Phyllostomidae bats, Sturnira lilium and Sturnira tildae had significantly higher capture rates in abandoned pasture, while Rhinophylla pumilio predominated in both Vismia‐ and Cecropia‐dominated regrowth. An hybrid multidimensional scaling ordination revealed significant differences in the bat community among the three types of secondary vegetation. Phyllostominae bats were more common and richer in the less‐disturbed areas of Cecropia‐dominated regrowth, while Stenodermatinae species were more captured in abandoned pastures. Our results suggest that the type of secondary vegetation, together with its land‐use history, affects bat community composition in the Central Amazon. The Phyllostominae subfamily (gleaning animalivores) was habitat selective and disappeared from areas experiencing constant disturbances. On the other hand, Stenodermatinae frugivorous bats often used and foraged in altered areas. We suggest that secondary vegetations in more‐advanced successional stages can be used to augment the total area protected by forest conservation units.

Reduced-impact logging and temporal activity of understorey bats in lowland Amazonia

Because global timber demands continue to threaten tropical rain forests, identification of sustainable-use forest management protocols that meet human needs while preserving biodiversity is critical. Reduced-impact logging (RIL) protocols are increasingly common in the tropics and may be a viable option for sustainable forest use; however, few studies have documented faunal responses to RIL. Moreover, evaluations of abundance or diversity may not be sufficient for a comprehensive understanding of faunal responses to human activities, especially in the short-term. We assessed the effects of RIL on the temporal activity patterns of abundant bats in lowland Amazonian rain forest. More specifically, we characterized temporal patterns of activity, overlap of temporal activity, and dispersion of activity modes for seven abundant bat species and for four common bat guilds in RIL forest and in undisturbed forest in Tapajós National Forest, Pará, Brazil. Temporal activity of aerial insectivores, nectarivores, and gleaning animalivores did not change in response to RIL. In contrast, three of five species of frugivores and frugivores as a group changed their patterns of temporal activity in response to RIL. RIL had a greater effect on temporal activity of frugivores that foraged in the understorey than on frugivores that foraged in multiple forest strata. Overlap of temporal activity of frugivores was greater than expected by chance in undisturbed forest, but was random in RIL forest. Changes in activity may be a response to a combination of increased predation risk and reduced distances of early evening flights between day roosts and feeding areas in RIL forest compared to undisturbed forest.

Changes in prey abundance unlikely to explain the demography of a critically endangered Central European bat

Summary Recognizing the factor(s) that caused a demographic crash is a prerequisite to the development of a tailored population restoration plan. While habitat destruction leaves little scope for population persistence, cryptic habitat deterioration (for example through reduction of food resources) may similarly render an area totally inhospitable, while it still appears to have a suitable habitat configuration. Most European bat species have undergone dramatic declines over the past decades. Once among the most widespread and abundant bat species of Central Europe, the lesser horseshoe bat, Rhinolophus hipposideros (Bechstein 1800), is today extinct in many regions. Although changes in the agricultural landscape have been suggested as the major cause of decline, recent studies have shown that this bat forages almost exclusively in woodland, a habitat that has increased in area across continental Europe over the past decades. This suggests that habitat eradication per se is unlikely to be the main cause of decline. But could cryptic habitat deterioration play a harmful role? We looked at the abundance of insect prey in woodland in the vicinity of colonial roosts with diverging demographic status (extinct, declining or recovering populations), both in the Swiss lowlands (Swiss Plateau) and in the Alps. We predicted that population size correlates positively with prey abundance. Diet composition mirrored local insect prey abundance, confirming an opportunistic foraging strategy. Prey abundance showed marked seasonal variation, but did not differ between sites harbouring extinct, declining or recovering populations. There was also no difference in food abundance between extinct populations in the lowlands and recovering populations in the Alps. Synthesis and applications. Cryptic habitat deterioration through a reduction in prey abundance is unlikely to preclude recolonization of abandoned areas by presently recovering populations. However, sufficient areas of natural forest should be preserved or created around potential nursery roosts. Moreover, connectivity between forest patches must be ensured (by creation of hedges and tree lines) to prevent any spatial gap in recolonization within semi‐open agricultural landscapes. Other threatened European forest bats may also benefit from these measures.