Absorption Of Mineral Elements Research Articles

Unlocking the power of short-chain fatty acids in ameliorating intestinal mucosal immunity: a new porcine nutritional approach.

Short-chain fatty acids (SCFAs), a subset of organic fatty acids with carbon chains ranging from one to six atoms in length, encompass acetate, propionate, and butyrate. These compounds are the endproducts of dietary fiber fermentation, primarily catalyzed by the glycolysis and pentose phosphate pathways within the gut microbiota. SCFAs act as pivotal energy substrates and signaling molecules in the realm of animal nutrition, exerting a profound influence on the intestinal, immune system, and intestinal barrier functions. Specifically, they contibute to 60-70% of the total energy requirements in ruminants and 10-25% in monogastric animals. SCFAs have demonstrated the capability to effectively modulate intestinal pH, optimize the absorption of mineral elements, and impede pathogen invasion. Moreover, they enhance the expression of proteins associated with intestinal tight junctions and stimulate mucus production, thereby refining intestinal tissue morphology and preserving the integrity of the intestinal structure. Notably, SCFAs also exert anti-inflammatory properties, mitigating inflammation within the intestinal epithelium and strengthening the intestinal barrier's defensive capabilities. The present review endeavors to synthesize recent findings regarding the role of SCFAs as crucial signaling intermediaries between the metabolic activities of gut microbiota and the status of porcine cells. It also provides a comprehensive overview of the current literature on SCFAs' impact on immune responses within the porcine intestinal mucosa.

Effects of cadmium and zinc interactions on the physiological biochemistry and enrichment characteristics of Iris pseudacorus

Compound pollution with cadmium (Cd) and zinc (Zn) is common in nature. The effects of compounded Cd and Zn on the growth and development of Iris pseudacorus in the environment and the plant’s potential to remediate heavy metals in the environment remain unclear. In this study, the effects of single and combined Cd and Zn stress on I. pseudacorus growth and the enrichment of heavy metals in I. pseudacorus seedlings were investigated. The results showed that under Cd (160 μM) and Zn (800 μM) stress, plant growth was significantly inhibited and photosynthetic performance was affected. Cd+Zn200 (160 μM + 200 μM) reduced the levels of malondialdehyde, hydrogen peroxide, and non-protein thiols by 31.29%, 53.20%, and 13.29%, respectively, in the aboveground tissues compared with levels in the single Cd treatment. However, Cd+Zn800 (160 μM + 800 μM) had no effect. Cd and Zn800 inhibited the absorption of mineral elements, while Zn200 had little effect on plants. Compared with that for Cd treatment alone, Cd + Zn200 and Cd+Zn800 reduced the Cd content in aboveground tissues by 54.15% and 49.92%, respectively, but had no significant effect on Cd in the root system. Zn significantly reduced the Cd content in subcellular components and limited the content and proportion of Cd extracted using water and ethanol. These results suggest that a low supply of Zn reduces Cd accumulation in aboveground tissues by promoting antioxidant substances and heavy metal chelating agents, thus protecting the photosynthetic systems. The addition of Zn also reduced the mobility and bioavailability of Cd to alleviate its toxicity in I. pseudacorus.

Physiological responses of low- and high-cadmium accumulating Robinia pseudoacacia-rhizobium symbioses to cadmium stress

The role of rhizobia in alleviating cadmium (Cd) stress in woody legumes is still unclear. Therefore, two types of black locust (Robinia pseudoacacia L.) with high and low Cd accumulation abilities were selected from 11 genotypes in China, and the effects of rhizobium (Mesorhizobium huakuii GP1T11) inoculation on the growth, CO2 and H2O gas exchange parameters, Cd accumulation, and the absorption of mineral elements of the high (SX) and low Cd-accumulator (HB) were compared. The results showed that rhizobium-inoculation significantly increased biomass, shoot Cd contents, Cd accumulation, root-to-shoot translocation factor (TF) and the absorption and accumulation of mineral elements in both SX and HB. Rhizobium-inoculation increased chlorophyll a and carotenoid contents, and the intercellular carbon dioxide concentrations in HB plants. Under Cd exposure, the high-accumulator SX exhibited a significant decrease in photosynthetic CO2 fixation (Pn) and an enhanced accumulation of Cd in leaves, but coped with Cd exposure by increasing chlorophyll synthesis, regulating stomatal aperture (Gs), controlling transpiration (Tr), and increasing the absorption and accumulation of mineral elements. In contrast, the low-accumulator HB was more sensitive to Cd exposure despite preferential accumulation of Cd in roots, with decreased chlorophyll and carotenoid contents, but significantly increased root biomass. Compared to the low-accumulator HB, non-inoculated Cd-exposed SX plants had higher chlorophyll contents, and rhizobium-inoculated Cd-exposed SX plants had higher Pn, Tr, and Gs as well as higher levels of P, K, Fe, Ca, Zn, and Cu. In conclusion, the high- and low-Cd-accumulator exhibited different physiological responses to Cd exposure. Overall, rhizobium-inoculation of black locust promoted the growth and heavy metal absorption, providing an effective strategy for the phytoremediation of heavy metal-contaminated soils by this woody legume.

Structural characteristics and biological activities of polysaccharides from barley: a review.

Barley (Hordeum vulgare L.) is rich in starch and non-starch polysaccharides (NSPs), especially β-glucan and arabinoxylan. Genotypes and isolation methods may affect their structural characteristics, properties and biological activities. The structure-activity relationships of NSPs in barley have not been paid much attention. This review summarizes the extraction methods, structural characteristics and physicochemical properties of barley polysaccharides. Moreover, the roles of barley β-glucan and arabinoxylan in the immune system, glucose metabolism, regulation of lipid metabolism and absorption of mineral elements are summarized. This review may help in the development of functional products in barley.

Exploring the remediation potential of Hydrangea macrophylla (Thunb.) Ser. in cadmium-contaminated soil by comparing cultivars and seedling age

The current situation regarding soil cadmium pollution in China is far from optimistic, necessitating the exploration of more viable Cd pollution-remediating plants. Several studies have shown that ornamental plants have excellent potential for application in soil pollution remediation. However, research on the Cd tolerance capacity of Hydrangea remains scarce, and the effects of plant age on heavy metal tolerance has not been thoroughly investigated. In this study, greenhouse pot experiments were conducted with artificially prepared Cd-polluted soil at various concentrations (0–240 mg·kg−1). Two cultivars of H.macrophylla, “Endless Summer” (H.'ES') and “Classic Rood” (H.'CR'), were cultivated in different Cd-contaminated soil levels for 60 d, after which the growth response, physiological changes, mineral element absorption, and Cd concentration of different plant parts were measured. The effect of plant age on the heavy metal absorption capacity of H. macrophylla was also explored. The results showed that under Cd stress, the antioxidant activity and penetration-regulating substance content of H. macrophylla increased, and the absorption of Mg, Mn, and Zn changed. Under all Cd treatments, Cd concentrations in the roots were higher than those in the shoots of the cultivars, with a bioconcentration factor of > 1 and a translocation factor of < 1. The tolerance to Cd of the H.'CR' was stronger than that of the H.'ES'. The Cd tolerance of H. macrophylla increased with seedling age. This study indicated that H. macrophylla is a suitable candidate for Cd phytostabilization and the remediation of Cd-polluted soils to limit harm caused by Cd flow in the ecosystem.

Contrasting effect of pristine, ball-milled and Fe–Mn modified bone biochars on dendroremediation potential of Salix jiangsuensis “172” for cadmium- and zinc-contaminated soil

Bone biochar (BC) has a high capacity for the immobilization of potentially toxic elements (PTEs); however, its effect on dendroremediation efficiency remains unclear. Therefore, this study aimed to determine the effects of various concentrations (0, 0.5, 1, and 2 wt%) of BC, ball-milled BC (MBC), and Fe–Mn oxide-modified BC (FMBC) on soil properties, plant growth, and metal accumulation in Salix jiangsuensis “172” (SJ-172) grown in cadmium (Cd)- and zinc (Zn)-contaminated soil. BC and MBC promoted the photosynthetic rate, mineral element absorption, and plant growth of SJ-172, whereas FMBC inhibited the growth of SJ-172. Different biochars greatly influenced the concentrations of Cd and Zn in tissues of SJ-172. BC and MBC elevated the Cd levels, whereas FMBC decreased the Cd content in the leaves, stems, and cuttings of SJ-172. Unlikely, BC, MBC and FMBC show no evident change to the Zn concentration in the aboveground tissues of SJ-172, while decreased root Cd and Zn content compared with the control. MBC, at a 2.0% application rate, significantly increased the translocation factors of Cd (55.0%) and Zn (40.87%), whereas BC and FMBC demonstrated no significant effects compared with the control (P > 0.05). Moreover, 2.0% BC and MBC increased Cd and Zn accumulation in SJ-172 by 28.40 and 41.14, and 25.89 and 36.16%, respectively, whereas 2.0% FMBC reduced Cd and Zn accumulation by 53.20% and 13.18 %, respectively, compared with the control. The phytoremediation potential of SJ-172 for Cd- and Zn-contaminated soils was enhanced by MBC and BC, whereas it was lowered by FMBC compared to the control. These results provide novel insights for the application of fast-growing trees assisted by biochar amendments in the dendroremediation of severely PTEs-contaminated soil.

Effects of Interaction of Protein Hydrolysate and Arbuscular Mycorrhizal Fungi Effects on Citrus Growth and Expressions of Stress-Responsive Genes (Aquaporins and SOSs) under Salt Stress.

Protein hydrolysates (PHs) and arbuscular mycorrhizal fungi (AMF) are environmentally friendly biostimulants that effectively promote crop growth and alleviate the damage from abiotic stress. However, the physiological and molecular regulatory mechanisms are still unclear. This study aimed to explore the effects of PHs and AMF on growth, mineral nutrient absorption, and expression of Aquaporins and SOSs in Goutoucheng (Citrus aurantium) under salt stress. Results showed that PH application and AMF inoculation significantly promoted plant growth and enhanced mineral element absorption and sodium effluxion in citrus under salt stress. The biomass, root activity, leaves mineral nutrition contents in PHs, AMF, and combined (PHs and AMF) treatments were significantly higher than those of control. Leaves sodium content in three treatments was significantly lower than in the control. AMF and combined treatments showed dominant effects than PHs alone. Besides, PHs interacted with AMF on growth, nutrient absorption, and sodium effluxion. Importantly, AMF and PHs induced stress-responsive genes. PIP1, PIP3, SOS1, and SOS3 expression in PHs and AMF treatments was significantly higher than control. Thus, it was concluded that AMF and PHs enhanced the salt tolerance of citrus by promoting nutrient absorption and sodium effluxion via up-regulating the expression of PIPs and SOSs. The mixed application of PHs and AMF had a better effect.

Production of Sweet Sorghum Bio-Feedstock on Technosol Using Municipal Sewage Sludge Treated with Flocculant, in Ukraine

This paper presents the influence of sewage sludge (SS) on the biometric parameters, absorption of mineral elements and thermal characteristics of sweet sorghum (Sorghum bicolor L.) grown on Technosol. Two types of sewage sludge were used: unmodified and modified with DAMET flocculant. Each type of sludge was applied in three doses (expressed as dry matter basis, DM): 20 t DM/ha, 40 t DM/ha and 60 t DM/ha, respectively. The yield of fresh biomass depended on the dose of sewage sludge: it increased by 14.5–41% and reached 104.6 t/ha after application of the sewage sludge with flocculant at a maximum rate of 60 t/ha. Sorghum biomass actively absorbs nutrients from the soil. Nitrogen was absorbed more actively; depending on the type and dose of sewage sludge application, its content in biomass increased from 12–40% (dose of 20 t/ha) to 80–112% (dose of 60 t/ha). By content in sorghum biomass, essential elements can be arranged in descending order as follows: Fe → Mn → Zn → Cu. Sorghum shows a low ability to accumulate some heavy metals in aboveground biomass. Despite the fact that large doses of sewage sludge contributed to an increase in the content of nickel, cadmium and lead in the substrate by 1.8–5.6 times, the ratio of the content of these elements in plants to the content in the substrate remained low. Sewage sludge affects the process of thermal degradation of sorghum biomass. The decomposition of the main components occurs at lower temperatures, and, as a rule, at higher rates. Sewage sludge (especially with flocculant) contributes to a more complete combustion of biomass. The results showed that sewage sludge with flocculant at a dose of 40 t/ha is the most optimal fertilizer option for growing sweet sorghum on such Technosols as loess-like loam.

Effect of low root-zone temperature on photosynthesis, root structure and mineral element absorption of tomato seedlings

Protected tomato cultivation is often subjected to low temperature stress in early spring season in north China. While low soil temperature returns slowly then low air temperature and causes damage to development of tomatoes, thereby reducing the growth of tomatoes, which seriously reduces the yield and quality of tomatoes. This study aimed to explore the effect of low root-zone temperature (LRT, i.e., 7 and 13℃) on the photosynthesis, root architecture, mineral use efficiency and aquaporins genes of tomato. Our findings indicated that LRT decreased the photosynthetic capacity accompanying with the reduction of CO2 assimilation. Meanwhile, a decrease in stomatal density and size occurred under low root-zone temperature. Stomatal conductance tended to reduce, which was unbeneficial to the diffusion of carbon dioxide and thus decreased photosynthesis. And there have the highest enzyme activity, and sucrose content compared to control. Root traits were remarkably affected by environment temperature. LRT delay root development processes and reduce the number and diameters of ducts in root and stem, thereby limiting mineral absorption and transport. Moreover, Under low root-zone temperature, the genes related to the aquaporin and element transport all showed varying degrees of response. Collectively, these results indicate that LRT may reduces the normal physiological activity of shoots by inhibiting the water transport, which inhibits the growth of tomato seedlings. Our research can provide detailed data to clarify the response characteristics of tomato to low root-zone temperature, and provide reference for low root-zone temperature response of tomato in practice.

Assessing the ecological risk of tritium and Carbon-14 discharge on cyanobacteria through metabolic profiling

The ecological risk posed by tritium (T) and carbon-14 (C-14) discharge from nuclear accidents has gained attention. This study evaluated the toxic impact of T and C-14 (at a concentration of 37 kBq/L for 15 days) on the cyanobacteria (Synechococcus elongatus). The results showed that the assimilation efficiency of cyanobacteria was significantly higher for C-14 than T, and the intracellular C-14 activity reached 30.62–40.58 kBq/kg. T and C-14 exposure had no significant effect on cell proliferation but impacted photosynthesis and respiration. T exposure increased the content of Ca, Mg, Na, P, K, and Mn, while C-14 exposure primarily affected trace element absorption in cyanobacteria. 31, 27, and 58 different metabolites (DEMs) were identified under T, C-14, and combined exposure conditions. These DEMs were enriched in the amino acid biosynthesis pathway, and nitrogen assimilation was one of the crucial pathways affected by T and C-14 exposure. The absorption of mineral elements by cyanobacteria was influenced by the variation in metabolites in the ABC transporter pathway caused by T and C-14 exposure. Our findings provide insights into the metabolic response of cyanobacteria to T and C-14 exposure and will help to guide the ecological risk evaluation of nuclear accidents.

Cultivar-specific response of rhizosphere bacterial community to uptake of cadmium and mineral elements in rice (Oryza sativa L.)

Toxic metal-contaminated farmland from Cadmium (Cd) can enhance the accumulation of Cd and impair the absorption of mineral elements in brown rice. Although several studies have been conducted on Cd exposure on rice, little has been reported on the relationship between Cd and mineral elements in brown rice and the regulatory mechanism of rhizosphere microorganisms during element uptake. Thus, a field study was undertaken to screen japonica rice cultivars with low Cd and high mineral elements levels, analyze the quantitative relationship between Cd and seven mineral elements, and investigate the cultivar-specific response of rice rhizosphere bacterial communities to differences in Cd and mineral uptake in japonica rice. Results showed that Huaidao-9 and Xudao-7 had low Cd absorption and high amounts of mineral nutrient elements (Fe, Zn, Mg, and Ca, LCHM group), whereas Zhongdao-1 and Xinkedao-31 showed opposite accumulation characteristics (HCLM group). Stepwise regression analysis showed that zinc, iron, and potassium are the key minerals that affect Cd accumulation in japonica rice and zinc was the most important factor, accounting for 68.99 %. The accumulation of Cd and mineral elements is potentially associated with rhizosphere soil bacteria. Taxa enriched in the LCHM rhizosphere (phyla Acidobacteriota and MBNT15) indicated the high nutrient characteristics of the soil and reduced activity of Cd in soil. The HCLM rhizosphere was highly colonized by metal-activating bacteria (Actinobacteria), lignin-degrading bacteria (Actinobacteria and Chlorofexi), and bacteria scavenging nutrients and trace elements (Anaerolinea and Ketobacter). Moreover, the differences in the uptake of Cd and mineral elements affected predicted functions of microbial communities, including sulfur oxidation and sulfur derivative formation, human or plant pathogen, and functions related to the iron oxidation and nitrate reduction. The results indicate a potential association of Cd and mineral elements uptake and accumulation with rhizosphere bacteria in rice, thus providing theoretical basis and a new perspective on the maintenance of rice security and high quality simultaneously.

Research Progress on the Effects of Selenium on the Growth and Quality of Tea Plants.

Selenium (Se) is an essential trace element for humans and animals, and it plays an important role in immune regulation and disease prevention. Tea is one of the top three beverages in the world, and it contains active ingredients such as polyphenols, theanine, flavonoids, and volatile substances, which have important health benefits. The tea tree has suitable Se aggregation ability, which can absorb inorganic Se and transform it into safe and effective organic Se through absorption by the human body, thereby improving human immunity and preventing the occurrence of many diseases. Recent studies have proven that 50~100.0 mg/L exogenous Se can promote photosynthesis and absorption of mineral elements in tea trees and increase their biomass. The content of total Se and organic selenides in tea leaves significantly increases and promotes the accumulation of polyphenols, theanine, flavonoids, and volatile secondary metabolites, thereby improving the nutritional quality of tea leaves. This paper summarizes previous research on the effects of exogenous Se treatment on the growth and quality of tea trees to provide a theoretical basis and technical support for the germplasm selection and exploitation of Se-rich tea.

Exogenous silicon alleviates the adverse effects of cinnamic acid-induced autotoxicity stress on cucumber seedling growth.

Autotoxicity is a key factor that leads to obstacles in continuous cropping systems. Although Si is known to improve plant resistance to biotic and abiotic stresses, little is known about its role in regulating leaf water status, mineral nutrients, nitrogen metabolism, and root morphology of cucumber under autotoxicity stress. Here, we used cucumber seeds (Cucumis sativus L. cv. “Xinchun No. 4”) to evaluate how exogenous Si (1 mmol L−1) affected the leaf water status, mineral nutrient uptake, N metabolism-related enzyme activities, root morphology, and shoot growth of cucumber seedlings under 0.8 mmol L−1 CA-induced autotoxicity stress. We found that CA-induced autotoxicity significantly reduced the relative water content and water potential of leaves and increase their cell sap concentration. CA-induced stress also inhibited the absorption of major (N, P, K, Ca, Mg) and trace elements (Fe, Mn, Zn). However, exogenous Si significantly improved the leaf water status (relative water content and water potential) of cucumber leaves under CA-induced stress. Exogenous Si also promoted the absorption of mineral elements by seedlings under CA-induced stress and alleviated the CA-induced inhibition of N metabolism-related enzyme activities (including nitrate reductase, nitrite reductase, glutamine synthetase, glutamate synthase, glutamate dehydrogenase). Moreover, exogenous Si improved N uptake and utilization, promoted root morphogenesis, and increased the growth indexes of cucumber seedlings under CA-induced stress. Our findings have far-reaching implications for overcoming the obstacles to continuous cropping in cucumber cultivation.

Effect of Hypoxic Stress and Levels of Mn on the Physiology and Biochemistry of Phyllostachys praecox.

Hypoxic environments have an adverse effect on the growth and development of P. praecox, and this is accompanied by the production of reducing substances such as Fe and Mn. In this study, the effect of hypoxic stress and Mn concentrations on leaf chlorophyll contents, root morphology, root activity, element absorption, antioxidant enzymes, and respiratory enzyme system of P. praecox were evaluated in a hydroponics environment. The results revealed that application of Mn2+ during hypoxic stress enhanced leaf chlorophyll contents and boosted up the indexes of the root system. The root activity of P. praecox was reduced with stresses of hypoxia. The treatment of Mn2+ initially improved and then decreased the root activity of P. praecox, and attained its maximum with application of 300 μmol/L Mn2+ compared with control. The indexes of antioxidant enzymes of P. praecox were higher than that of 8 mg/L oxygen concentrations except for variable superoxide dismutase (SOD) in the treatment of 300 μmol/L Mn2+ with hypoxia stress. The application of Mn had inhibited the absorption of mineral elements in P. praecox. The activities of pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH), and lactic dehydrogenase (LDH) were initially improved and then diminished with hypoxia stress. It is concluded that hypoxia is a key factor affecting the growth and degradation of P. praecox, while combining it with the increase of Mn concentration enhances the damage to Phyllostachys pubescens. Our research is helpful for the sustainable management and scientific fertilization management of Phyllostachys praecox.

Effect of Heating Time of Cottonseed Meal on Nutrient and Mineral Element Digestibility in Chicken (Based on Cottonseed Meal Replaced with All Soybean Meal).

Simple SummaryAt present, we are faced with the problem of a feed protein resource shortage. Cottonseed meal is a potential alternative protein source for use in poultry diets. High-temperature treatment is a common processing method of cottonseed meal. Our experiment was conducted to estimate the effects of heating time of cottonseed meal on nutrient digestibility and mineral element absorption of chicken.A digestibility test was conducted to estimate the effects of the heating time of cottonseed meal on nutrient digestibility and mineral element absorption in chicken. A total of 36, 45-week-old healthy New Yangzhou chickens with similar body weight were randomly divided into 6 groups with 6 replicates per group and one chicken per replicate. The chickens in Group A (control group) were fed the corn-soybean meal diet. The chickens in Groups B, C, D, E, and F (experimental groups) were fed the cottonseed meal to replace all soybean meal. The cottonseed meal in the experimental groups was treated with wet heating. The heating temperature was set at 120 °C, and the humidity was set at 50%. The heating time was set to 10, 15, 20, 25, and 30 min successively. The trial period was 4 day. The digestibility of crude protein, metabolic energy, and dry matter was highest using wet-heat treating for 15 min (p < 0.05). The digestibility of Fe increased significantly from 66.78% to 70.39% when the heating time of cottonseed meal was prolonged from 10 min to 30 min (p < 0.05). Compared with Group A, the digestibility of Zn was increased in Groups B and C, and then there was a decrease in Group D. Finally, the digestibility was increased again in Group F. The opposite pattern was shown in the digestibility of Cu and Zn. There was no significant effect of wet heat treatment time on the digestibility of calcium, phosphorus, and manganese (p > 0.05). When cottonseed meal is considered as feed for 45-week-old chickens, processing conditions of 120 °C and 50% humidity for 15 min are recommended.

Effects of Exogenous Phthalic Acid on Seed Germination, Root Physiological Characteristics, and Mineral Element Absorption of Watermelon

To understand the effect of exogenous PA on the watermelon root system, the watermelon variety ‘Zaojia 84–24’ was used as experimental material. This study investigated the effects of allelochemicals DIBP and DOP at varying different concentrations (0, 0.05, 0.1, 0.5, 1, and 4 mmol·L−1) on the physiological characteristics and mineral content of watermelon roots. The results revealed that proper PA treatment concentrations (0.05~0.1 mmol·L−1) promoted seed germination, increased the number of RBCs and the survival rate of RBCs, and enhanced the activities of PME and dehydrogenase in watermelon roots. In addition, proper PA treatment concentrations (0.05~0.1 mmol·L−1) promoted the activities of SOD, POD, CAT, and NR in watermelon roots. The contents of MDA and soluble protein were increased at 0.05~4 mmol·L−1 PA. In addition, proper PA treatment concentrations promoted the absorption and accumulation of P, K, Ca, Fe, Cu, and Zn elements in watermelon roots. These results indicate that PA at a concentration of 0.05~0.5 mmol·L−1 can promote watermelon seed germination, improve antioxidant enzyme activity of watermelon roots, and maintain normal physiological activities of watermelon by affecting absorption and accumulation of mineral elements in the root system.

Genome-wide identification and expression analysis of HAK/KUP/KT potassium transporter provides insights into genes involved in responding to potassium deficiency and salt stress in pepper (Capsicum annuum L.).

The online version contains supplementary material available at 10.1007/s13205-022-03136-z.

Nutritional Properties of Larval Epidermis and Meat of the Edible Insect Clanis bilineata tsingtauica (Lepidoptera: Sphingidae).

Insects represent a sustainable, protein-rich food source widely consumed in Asia, Africa, and South America. Eating Clanis bilineata tsingtauica Mell is common in the eastern part of China. A comparative characterization of nutrients in the meat and epidermis of C. bilineata tsingtauica was performed in this study. The results showed this insect to be high in nutrients, particularly in the epidermis where protein total was 71.82%. Sixteen different amino acids were quantified in C. bilineata tsingtauica, and the ratio of essential to nonessential amino acids in the epidermis and meat was 68.14% and 59.27%, respectively. The amino acid composition of C. bilineata tsingtauica is balanced, representing a high-quality protein source. Eight minerals were quantified in C. bilineata tsingtauica, including four macro and four trace elements. Fe in the epidermis and Zn in the meat were abundant at 163.82 and 299.31 μg/g DW, respectively. The presence of phytic acid impacted the absorption of mineral elements in food. We also detected phytic acid in C. bilineata tsingtauica. The molar ratio of phytic acid to zinc (PA/Zn) in C. bilineata tsingtauica was very low (3.28) compared to Glycine max and Cryptotympana atrata, which indicated that mineral utilization was high. In conclusion, this study confirms that C. bilineata tsingtauica is a highly nutritious food source for human consumption, and the results provide a basis for further consumption and industrialization of this edible insect.

Meta-analysis of the role of zinc in coordinating absorption of mineral elements in wheat seedlings

BackgroundZinc (Zn) is an important nutrient for human beings, which is also an essential micronutrient for crop growth. This study investigated the role of Zn in coordinating the mineral elements absorption in modern wheat (Triticum aestivum L.) cultivars with a new developed method.ResultsA method was developed, and showed a robust capability to simultaneously investigate seven mineral elements uptake in wheat seedling. With this method, we found low Zn supply (< 1 μM) promoted the absorption of potassium (K), magnesium (Mg) and manganese (Mn) in wheat seedling, while high Zn supply (> 1 μM) significantly inhibited the absorption of these elements. Cultivars with the green genes (Rht-B1b and Rht-D1b) showed a higher uptake capability on ammonium (NH4+), and cultivars with Rht-B1b allele can uptake more phosphors (P), K, calcium (Ca), Mn and Zn compared to cultivars with Rht-D1b. Further analysis indicated higher uptake capability of NH4+ in cultivars contained Rhts was independent of Zn.ConclusionThe key role of Zn in coordinating for mineral elements absorption was identified in modern wheat cultivars, providing the reference for Zn application in wheat. Meanwhile, this study provides a robust method for quantifying the absorption of mineral elements, which may be adopted into the broadly investigations on the coordinated nutrients absorption of plant.

Effects of Lead (Pb) and Benzo [a] Pyrene (B[a]P) and their Combined Exposure on Element Accumulation in Ryegrass (Lolium perenne L.).

It was observed in this work that application of Pb and B[a]P co-exposure significantly (p < 0.05) reduced Pb content in ryegrass leaves and roots. The effect of Pb dominated the change of N, P, K, Cu, and Cr content in leaves and roots of ryegrass under joint stress of Pb and B[a]P. Principal component analysis showed that the foliar spraying of 400μmol L-1 Pb and 80μmol L-1 B[a]P had the best effect on improving the mineral element absorption under combined pollution. Ryegrass has strong resistance and certain Pb and B[a]P absorptive capacities, and can resist combined contamination by transferring N, P, K, Zn, Cu, and Cr contents between the overground and the root. These results highlight the potential capacity of ryegrass for use in the phytoremediation of soils contaminated by Pb and B[a]P.