Absence Of Tetracycline Research Articles

Electrochemical Determination of Tetracycline Using the Synergy of a Molybdenum (IV) Sulfide-Thionine Nanocomposite with an Aptamer by Differential Pulse Voltammetry (DPV)

An electrochemical aptasensor was established for the fast and sensitive determination of tetracycline based on the synergistic effect of MoS2-thionine nanocomposite and aptamer. The glassy carbon electrode was coated with MoS2 nanosheets of thionine to immobilize the tetracycline aptamer. In the absence of tetracycline, large quantities of thionin were loaded on the surface of the MoS2 nanosheet to serve as the electrochemical probe. After tetracycline was added to the surface of the electrode, an aptamer analyte complex was produced on the modified electrode. Through intercalation and electrostatic interactions, the complex was substantially adsorbed onto thionine, and the peak currents of the redox signal of thionine decreased. Therefore, the concentration of tetracycline was monitored by the change in signal intensity. Under optimized conditions, the developed electrochemical strategy with MoS2-thionine composite and aptamer exhibited an excellent linear detection range of 1.0 nM–1.0 μΜ and a low detection limit of 0.6 nM with suitable selectivity and stability. Therefore, the fabricated platform offers great potential for food safety, medical detection, and drug analysis.

Iron-loaded sludge biochar alleviates the inhibitory effect of tetracycline on anammox bacteria: Performance and mechanism

The anaerobic ammonia oxidation (anammox) process is sensitive to environmental pollutants, such as antibiotics. In this study, the harmful effect of tetracycline (TC) on the performance of an anammox reactor and the mitigation of TC inhibition by iron-loaded sludge biochar (Fe-BC) were studied by analyzing extracellular polymeric substances (EPS), microbial community structure and functional genes. The total inorganic nitrogen (TIN) removal rate of the TC reactor was reduced by 5.86% compared to that of the control group, while that of the TC + Fe-BC reactor improved by 10.19% compared to that of the TC reactor. Adding Fe-BC increased the activity of anammox sludge by promoting the secretion of EPS (including protein, humic acids and c-Cyts). The results of the enzymolysis experiment showed that protein can improve the activity of anammox sludge, while the ability of polysaccharide to improve the activity of anammox was related to the treated enzymes. In addition, Fe-BC alleviated the inhibitory effect of TC by mediating the anammox electron transfer process. Furthermore, Fe-BC increased the absolute abundance of hdh and hzsB by 2.77 and 1.18 times compared to the TC reactor and improved the relative abundance of Candidatus Brocadia in the absence of TC. The addition of Fe-BC is an effective way to alleviate the inhibitory effect of TC on the anammox process.

Mannan based prebiotics modulate growth rate and energy phenotype of tetracycline resistant E. coli

Unsustainable antimicrobial use in industrial agriculture has contributed to the rise in antimicrobial resistance and there is an urgent need to find alternative and more sustainable strategies to traditional antimicrobials. Prebiotics, such as mannan-rich fraction (MRF), a cell wall product from Saccharomyces cerevisiae, have demonstrated an ability to alter the growth of antibiotic susceptible and resistant Escherichia coli and improve the efficacy of antibiotics through modulation of cellular activity. In this study the impact of mannan based prebiotics on growth and respiration of E. coli was assessed by observing microbial growth, oxygen consumption rate and extracellular acidification rate in the presence and absence of tetracycline. The findings further demonstrate the capabilities of MRF with respect to improving microbial antibiotic sensitivity, particularly in resistant strains. This potentially enables a more efficient control of resistant pathogens with food safety implications and promotion of more sustainable use of antibiotics in animal production systems.

The impact of glutaraldehyde based footbaths on Dichelobacter nodosus prevalence and the antimicrobial resistant community of the ovine interdigital skin

Ovine footrot, is a highly contagious polymicrobial bacterial infection, primarily caused by Dichelobacter nodosus. Preventative bactericidal footbaths are commonly used in the sheep industry to reduce the spread of bacteria. However, their effect on the bacterial community is poorly understood. This is the first study to investigate the impact of 2% Digicur (ProGiene,UK) footbath on the bacterial community of the ovine interdigital skin following a common UK footbathing routine. Swab samples were analysed by qPCR to determine prevalence and load of D. nodosus and numerated on MacConkey agar in the presence or absence of tetracycline and ampicillin to determine phenotypic antimicrobial resistance. Metagenomics were used to determine the impact of a single footbath on the bacterial community and genotypic antimicrobial resistance. The results suggest 2% Digicur is ineffective at reducing the load of D. nodosus when applied as a one off or weekly footbath, however sheep may act as a reservoir for multi-drug resistant bacteria creating opportunities to spread antimicrobial resistance to other sheep and their environment.

A novel colorimetric aptasensor for sensitive tetracycline detection based on the peroxidase-like activity of Fe3O4@Cu nanoparticles and "sandwich" oligonucleotide hybridization.

Anovel colorimetric aptasensor has been developedfor highly sensitive tetracycline (TC) detection based on the peroxidase-like activity of Fe3O4@Cu nanoparticles and "sandwich" oligonucleotide hybridization. The Fe3O4@Cu nanoparticles with high peroxidase-like activity were successfully synthesized under mild conditions. Then, a "sandwich" oligonucleotide hybridization probe (a short amino-modified complementary sequence of a portion of the TC aptamer (cDNA1), TC aptamers, and a long complementary to 5' terminal TC aptamer sequence (cDNA2)) was created in 96-wells plates via DNA hybridization in the absence of TC from the detection system. The unique "sandwich" oligonucleotide hybridization probe adsorbed large numbers of Fe3O4@Cu nanozymes while further enhancing its peroxidase-like activity. Based on the 3,3',5,5'-tetramethylbenzidine (TMB)-hydrogen peroxide (H2O2) reporting system, the blue color of the solution decreased linearly with the increase ofTC concentration, ranging from 10-3 to 103μg/L with an ultralow limit of detection (LOD) of 0.91ng/L (2pM). Theproposed method was successfully applied to detect TC in spiked milk samples, with recoveries of 81.8 to 112%, demonstrating the excellent potential for highly sensitive TC detection in milk.

Enhanced production of microalgae-originated photosensitizer by integrating photosynthetic electrons extraction and antibiotic induction towards photocatalytic degradation of antibiotic: A novel complementary treatment process for antibiotic removal from effluent of conventional biological wastewater treatment

Antibiotic residues in effluents from bio-treated wastewaters are mainly responsible for the spread of antibiotic resistance genes in the environment. Conventional physicochemical treatments are thought to be unsustainable due to high energy consumption, large consumption of chemicals and environmental unfriendly processing step. In this study, a novel approach by integrating photosynthetic electrons extraction from microalgae with antibiotic induction was used to enhance the production of microalgae-originated photosensitizer for photolytic removal of antibiotic residues in effluents from conventional bio-treated wastewaters. Results showed that the accumulation of photoactive substances in extracellular polymeric substance (EPS) of chlorella vulgaris was positively related to the amounts of photosynthetic electrons extracted by the electrode which is a potential-dependent process and can be further enhanced by tetracycline (TC) induction. The protein and humic acid which are considered two main photoactive substances in EPS produced at 0.6 V accumulated to a high level of 320 and 24 μg/cm3 and were further increased to 380 and 48 μg/cm3 when TC was added which were 4.7 and 6.4-folds higher than that produced at potential free in the absence of TC. The EPS produced at 0.6 and 0.8 V led to 1.34 and 1.53-fold acceleration in photosensitized degradation of TC compared to that of EPS free in secondary effluent of municipal wastewater treatment plant. The complex heterocyclic ring structure of TC was broken down into simple monocyclic aromatic compounds, indicating a marked reduction in biotoxicity and recalcitrance. The hydroxyl radical played a main role for the photolysis of TC followed by singlet oxygen. This technology provides a new alternative to conventional physicochemical treatment as complementary treatment processes for biological wastewater treatment in terms of antibiotics removal.

Psidium Guajava L. Extract Against Minimum Inhibitory Concentration of Staphylococcus Aureus

The aim of this research was to evaluate the effects on the growth of Staphylococcus Aureus bacteria in vitro of antibacterial activity of guayava leaf extract (Psidium guajava L.) and guavava leaf extract impact on the growth of staphylococcus Aureus bacteria at different laboratory concentrations. In this experiment, S. Aureus bacteria are being treated to guava leaf extract to identify the lowest inhibitory power for well techniques, and the results have been recorded. The Guava leaf extract (Psidium Guajava L) against Staphylococcus aureus was found to have an inhibitory zone diameter of mm in the absence of tetracycline, and DMSO as a negative control. Findings were observed using the disk diffusion technique or paper disc with concentrations of 1 percent, 3 percent, 5 percent, and 7 percent. Saponins, saponins, alkaloids, and flavonoids found in guava leaves are responsible for their antibacterial effects. Saponins have the potential to disturb the integrity of bacterial cell membranes. This results in bacterial cell membrane destruction, releasing essential components from the cell

A conditional female lethal system for genetic suppression of the global fruit crop pest Drosophila suzukii.

Drosophila suzukii (Matsumura, 1931, Diptera: Drosophilidae) is a global pest of soft-skinned fruits such as blueberries, cherries and raspberries. Also known as spotted-wing drosophila, D. suzukii is native to Asia but is now widely distributed in the Americas and Europe, and presents a serious challenge for growers. Genetic control strategies offer an environmentally friendly approach for the control of D. suzukii. In this study, we developed transgenic strains of D. suzukii that carry dominant conditional female lethal transgenes. When raised in the absence of tetracycline, female D. suzukii die. We show that repeated releases of an excess of transgenic males can suppress D. suzukii populations in laboratory cage trials. Our data suggest that the transgenic strain could provide an effective approach for control of this invasive pest of soft-skinned fruits.

A transgenic female killing system for the genetic control of Drosophila suzukii

The spotted wing Drosophila (Drosophila suzukii) is an invasive pest of soft-skinned fruit crops. It is rapidly transmitted in Europe and North America, causing widespread agricultural losses. Genetic control strategies such as the sterile insect technique (SIT) have been proposed as environment-friendly and species-restricted approaches for this pest. However, females are inefficient agents in SIT programs. Here we report a conditional female-killing (FK) strategy based on the tetracycline-off system. We assembled sixteen genetic constructs for testing in vitro and in vivo. Twenty-four independent transgenic strains of D. suzukii were generated and tested for female-specific lethality. The strongest FK effect in the absence of tetracycline was achieved by the construct containing D. suzukii nullo promoter for early gene expression, D. suzukii pro-apoptotic gene hidAla4 for lethality, and the transformer gene intron from the Mediterranean fruit fly Ceratitis capitata for female-specific splicing. One strain carrying this construct eliminated 100% of the female offspring during embryogenesis and produced only males. However, homozygous females from these FK strains were not viable on a tetracycline-supplemented diet, possibly due to the basal expression of hidAla4. Potential improvements to the gene constructs and the use of such FK strains in an SIT program are discussed.

An early female lethal system of the New World screwworm, Cochliomyia hominivorax, for biotechnology-enhanced SIT

BackgroundThe New World Screwworm fly (NWS), Cochliomyia hominivorax, is an ectoparasite of warm-blooded animals and a major pest of livestock in parts of South America and the Caribbean where it remains endemic. In North and Central America it was eradicated using the Sterile Insect Technique (SIT). A control program is managed cooperatively between the governments of the United States and Panama to prevent the northward spread of NWS from infested countries in South America. This is accomplished by maintaining a permanent barrier through the release of millions of sterile male and female flies in the border between Panama and Colombia. Our research team demonstrated the utility of biotechnology-enhanced approaches for SIT by developing a male-only strain of the NWS. The strain carried a single component tetracycline repressible female lethal system where females died at late larval/pupal stages. The control program can be further improved by removing females during embryonic development as larval diet costs are significant.ResultsThe strains developed carry a two-component system consisting of the Lucilia sericata bottleneck gene promoter driving expression of the tTA gene and a tTA-regulated Lshid proapoptotic effector gene. Insertion of the sex-specifically spliced intron from the C. hominivorax transformer gene within the Lshid gene ensures that only females die when insects are reared in the absence of tetracycline. In several double homozygous two-component strains and in one “All-in-one” strain that had both components in a single construct, female lethality occurred at the embryonic and/or first instar larval stages when raised on diet without tetracycline. Laboratory evaluation for phenotypes that are relevant for mass rearing in a production facility revealed that most strains had fitness characteristics similar to the wild type J06 strain that is currently reared for release in the permanent barrier. Testing of an “All in one” strain under mass rearing conditions showed that the strain maintained the fitness characteristics observed in small-scale rearing.ConclusionsThe early female lethal strains described here could be selected by the NWS Control Program for testing at large scale in the production facility to enhance the efficiency of the NWS eradication program.

Highly sensitive detection of multiple antibiotics based on DNA tetrahedron nanostructure-functionalized magnetic beads

Functional DNAs-functionalized magnetic beads (MBs) offer great potential in bioanalysis field because of their target recognition and magnetic separation functions. However, the recognition capability and hybridization affinity of DNA probes often suffer from limited available space, poor probe conformation and non-selective adsorption. To overcome these limitations, we herein used aptamer-pendant DNA tetrahedron nanostructure-functionalized MBs (TETapt-tet MBs) to develop a target-response fluorescence method with tetracycline (TET) as a model. In the absence of TET, 6-carboxy-X-rhodamine-labeled complementary DNAs (ROX-cDNAs) were assembled on the surface of MBs. Upon the addition of target TET, the ROX-cDNAs were separated and released from the MBs to generate fluorescence signal. The limit of detection and limit of quantification for TET were found to be 6 pg mL−1 and 20 pg mL−1, respectively. Compared with ssDNA-functionalized MBs surface, the designed DNA tetrahedron nanostructure-based surface could decrease the hybridization time and reduce false positives, ensuring the accuracy of TET detection in complex samples. The presented method was successfully employed for TET detection in honey samples. Moreover, this functionalization strategy could be extended to detect multiple antibiotics by simply substituting different aptamer sequences. Therefore, the proposed method has great potential in the field of food safety and public health.

Improved transgenic sexing strains for genetic control of the Australian sheep blow fly Lucilia cuprina using embryo-specific gene promoters.

For genetic approaches for controlling insect pests such as the sterile insect technique (SIT), it is advantageous to release only males as females are ineffective as control agents and they consume about 50% of the diet. Here we developed tetracycline-repressible Lucilia cuprina transgenic strains in which adult females were fully fertile and viable on a diet that lacked tetracycline and all of their female offspring died at the embryo stage. The transgenic strains are an improvement over the strains we developed previously, which had the disadvantage that adult females on diet without tetracycline were sterile and died prematurely. This was possibly due to the low level expression of the effector gene in ovaries. In the strains developed in this study, the early promoters from L. cuprina nullo or Cochliomyia macellaria CG14427 genes were used to drive the tetracycline transactivator (tTA) expression in the early embryo. In the absence of tetracycline, tTA activates expression of the proapoptotic gene Lshid which contains a female-specific intron. Consequently, only females produce active HID protein and die at the embryo stage. Crossing the tTA-expressing driver lines with an RFPex reporter line confirmed that there was no expression of the effector gene in the ovary. These new embryonic L. cuprina transgenic sexing strains hold great promise for genetic control programs and the system reported here might also be transferable to other major calliphorid livestock pests such as the New World screwworm, Cochliomyia hominivorax.

Ratiometric detection of tetracycline based on gold nanocluster enhanced Eu3+ fluorescence

Tetracycline (TC), a widely-used antibiotic to treat bacterial infections, combines with Eu3+ to form a stable EuTC complex that has a low fluorescence (FL) yield because of Eu3+ coordination with water molecules. We report a simple, label-free ratiometric fluorescent platform for sensitively and selectively sensing of TC, using L-histidine caped gold nanoclusters (His-AuNCs) as a FL indicator and an enhancer of Eu3+ FL. The His-AuNCs were prepared via chemical reduction of Au(III) by L-histidine, which was a reducer and a protecting agent. The His-AuNCs exhibited good photostability, outstanding stability toward high ionic strengths, storage stability, and favorable optical properties. In the absence of TC, the AuNCs-Eu3+ system displays strong FL emission at 475 nm (F475) from the His-AuNCs and weak FL at 620 nm from Eu3+ (F620) with excitation at 375 nm. TC quenches the His-AuNC FL and greatly enhances the Eu3+ emission. This is attributed to an enhancement of the EuTC complex fluorescence by the His-AuNCs. Thus, a ratiometric F620/F475 FL signal can be used for TC detection by simply mixing AuNCs and Eu3+. Under the optimized conditions, the linear range from 10 nM to 60 μM and a detection limit of 4 nM (S/N = 3) for TC were obtained. The application of the assay platform for the detection of TC in environmental and biological samples was demonstrated. The sensing platform has advantages of easy preparation, rapid response, high sensitivity, and good selectivity.

Influence of Mg2+ on the conformational flexibility of a tetracycline aptamer.

The tetracycline-binding RNA aptamer (TC-aptamer) is a synthetic riboswitch that binds the antibiotic tetracycline (TC) with exceptionally high affinity. Although a crystal structure exists of the TC-bound state, little is known about the conformational dynamics and changes upon ligand binding. In this study, pulsed electron paramagnetic resonance techniques for measuring distances (PELDOR) in combination with rigid nitroxide spin labels (Çm spin label) were used to investigate the conformational flexibility of the TC-aptamer in the presence and absence of TC at different Mg2+ concentrations. TC was found to be the essential factor for stabilizing the tertiary structure at intermediate Mg2+ concentrations. At higher Mg2+ concentrations, Mg2+ alone is sufficient to stabilize the tertiary structure. In addition, the orientation of the two spin-labeled RNA helices with respect to each other was analyzed with orientation-selective PELDOR and compared to the crystal structure. These results demonstrate for the first time the unique value of the Çm spin label in combination with PELDOR to provide information about conformational flexibilities and orientations of secondary structure elements of biologically relevant RNAs.

Broadly Neutralizing Anti-HIV-1 Antibodies Do Not Inhibit HIV-1-ENV-Mediated Cell-Cell Fusion

PG9, PG16, PGT121, and PGT145 antibodies were identified from culture media of activated memory B-cells of an infected donor and shown to neutralize many HIV-1 strains. Since HIV-1 spreads via both free virions and cell-cell fusion, we examined the effect of the antibodies on HIV-1 Env-mediated cell-cell fusion. Clone69TRevEnv cells that express Env in the absence of tetracycline were labeled with Calcein-AM Green, and incubated with CD4+ SupT1 cells labeled with CellTrace Calcein Red-Orange, with or without antibodies. Monoclonal antibodies PG9, PG16, 2G12, PGT121, and PGT145 (at up to 50 μg/mL) had little or no inhibitory effect on fusion between HIV-Env and SupT1 cells. By contrast, Hippeastrum hybrid agglutinin completely inhibited fusion. Our results indicate that transmission of the virus or viral genetic material would not be inhibited by these broadly neutralizing antibodies. Thus, antibodies generated by HIV-1 vaccines should be screened for their inhibitory effect on Env-mediated cell-cell fusion.

Regulating the UAS/GAL4 system in adult Drosophila with Tet-off GAL80 transgenes.

The UAS/GAL4 system is the most used method in Drosophila melanogaster for directing the expression of a gene of interest to a specific tissue. However, the ability to control the temporal activity of GAL4 with this system is very limited. This study constructed and characterized Tet-off GAL80 transgenes designed to allow temporal control of GAL4 activity in aging adult muscles. By placing GAL80 under the control of a Tet-off promoter, GAL4 activity is regulated by the presence or absence of tetracycline in the diet. Almost complete inhibition of the expression of UAS transgenes during the pre-adult stages of the life cycle is obtained by using four copies and two types of Tet-off GAL80 transgenes. Upon treatment of newly emerged adults with tetracycline, induction of GAL4 activity is observed but the level of induction is influenced by the concentration of the inducer, the age, the sex and the anatomical location of the expression. The inhibition of GAL4 activity and the maintenance of induced expression are altered in old animals. This study reveals that the repressive ability of GAL80 is affected by the age and sex of the animal which is a major limitation to regulate gene expression with GAL80 in aged Drosophila.

Transcriptional effects of a positive feedback circuit in Drosophila melanogaster

BackgroundSynthetic systems that use positive feedback have been developed to control human disease vectors and crop pests. The tTAV system, which has been deployed in several insect species, relies on a positive feedback circuit that can be inhibited via dietary tetracycline. Although insects carrying tTAV fail to survive until adulthood in the absence of tetracycline, the exact reason for its lethality, as well as the transcriptomic effects of an active positive feedback circuit, remain unknown.ResultsWe engineered the tTAV system in Drosophila melanogaster and investigated the effects of tTAV genome integration locus on the whole fly transcriptome during larval and adult life stages in four transgenic fly strains using gene expression microarrays. We found that while there were widespread effects on the transcriptome, the gene expression differences after removal of tetracycline were not consistent between integration sites. No specific region of the genome was affected, no common set of genes or pathways, nor did the integration site affect the transcripts in cis.ConclusionAlthough the positive feedback tTAV system is effective at killing insect larvae regardless of where it is inserted in the genome, it does not exhibit a specific, consistent transcriptional signature. Instead, each insertion site is associated with broad, but different, transcriptional effects. Our results suggest that lethality may not be caused by a direct effect on transcription of a set of key genes or pathways. Instead, we propose that rather than a specific action of a tTAV protein, it is the stochastic transcriptional effects specific to each insertion site that contribute to the tTAV-induced mortality.

Exposure to genetically engineered olive fly (Bactrocera oleae) has no negative impact on three non-target organisms

Bactrocera oleae (Diptera: Tephritidae) remains a major pest of olive fruit production worldwide. Current pest management programs largely depend on chemical insecticides, resulting in high economic and environmental costs. Alternative pest control approaches are therefore highly desirable. We have created a conditional female-specific self-limiting strain of B. oleae (OX3097D-Bol) that could be applied for sustainable pest control. OX3097D-Bol olive fly carries a fluorescent marker (DsRed2) for identification and a self-limiting genetic trait that is repressed by tetracycline. In the absence of tetracycline, the tetracycline transactivator (tTAV) accumulates, resulting in female death at larvae and early pupal stages. The aim of this study was to evaluate the impact of genetically engineered OX3097D-Bol olive fly on three non-target organisms that either predate or parasitize olive flies, one from the guild of parasitoids (Psyttalia concolor) and two from the guild of predators (Pardosa spider species and the rove beetle Aleochara bilineata). No significant negative effect was observed on life history parameters, mortality and reproductive capacity of the non-target organisms studied. These results suggest that potential exposure to DsRed2 and tTAV gene products (e.g. mRNA and encoded proteins) would have a negligible impact on on-target organisms in the guilds or predators and parasitoids.

Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus

Hepatitis B virus (HBV) is the smallest partially double-stranded DNA virus known to infect humans. Worldwide, more than 50% of hepatocellular carcinoma (HCC) cases are related to chronic Hepatitis B. Development of HCC from normal liver cells is characterized by changes in cell surface N-glycans, which can promote the invasive behavior of tumor cells, leading ultimately to the progression of cancer. However, little is understood about the cell surface N-glycans of HBV-infected liver cells. We try to address this by taking advantage of the HepAD38 cell line, which can replicate HBV in the absence of tetracycline [tet(−)] in growth medium. In the presence of tetracycline [tet(+)], this cell line is free from the virus due to the repression of pregenomic (pg) RNA synthesis. In culture medium without tetracycline, cells express viral pgRNA and start to secrete virions into the supernatant. Here we studied the expression of glycosyltransferases and the cell surface N-glycan composition of tet(+) and tet(−) HepAD38. Among the glycosyltransferases upregulated by the expression of HBV were GnT-II, GnT-IVa, ST6Gal1, and GnT-V, whereas GnT-I, GnT-III, β4GalT1, and FUT8 were downregulated. About one-third of the total cell surface N-glycans found on tet(−)HepAD38 were sialylated. As for tet(+)HepAD38, sialylation was 6% lower compared to the tet(−) cells. Neither treatment changed the cell surface N-glycans expression of the total complex type or the total fucosylated type, which were about 50% or 60%, respectively. Our results showed that the expression of HBV triggers higher sialylation in HepAD38 cells. Altogether, the results show that HBV expression triggered the alteration of the cell surface N-glycosylation pattern and the expression levels of glycosyltransferases of HepAD38 cells.

Label-free fluorescent strategy for sensitive detection of tetracycline based on triple-helix molecular switch and G-quadruplex

In this assay, a label-free fluorescent sensing platform based on triple-helix molecular switch (THMS) and G-quadruplex was developed for the detection of tetracycline. We demonstrated this approach by using THMS, which consists of a central section with a shortened 8-mer aptamer sequence with high affinity to tetracycline and flanked by two arm segments. G-rich oligonucleotide can specifically bind to thioflavin T (ThT) as a signal transduction probe (STP). In the absence of tetracycline, THMS remains stable, the fluorescence of background is low. By the addition of target tetracycline, the aptamer-target binding results in the formation of a structured aptamer-target complex, which disassembles the THMS and releases the STP. The free STP self-assembles into G-quadruplex and specifically binds to ThT which generates a obvious fluorescence enhancement. Using the triple-helix molecular switch, the developed aptamer-based fluorescent sensing platform showed a linear relationship with the concentration of tetracycline ranging from 0.2 to 20.0nmol/L. The detection limit of tetracycline was determined to be 970.0pmol/L. The assay avoids complicated modifications or chemical labeling, making it simple and cost-effective. So, it is expected that this aptamer-based fluorescent assay could be extensively applied in the field of food safety inspection.