Blood Grouping Research Articles

Periodontal Diseases and Salivary Secretion of ABH Blood Antigens in Yemeni Patients

This study explored the secretion trait as a risk factor for periodontal disease progression. 162 patients suffering from periodontal disease attended the Outpatient Dental Clinics at the Faculty of Dentistry, Ibb University, and 69 healthy volunteers were enrolled in this study. Clinical evaluation for periodontal disease was carried out according to international guidelines and expressed by pocket probing depth (PPD), bleeding on probing (BOP), and clinical attachment level (CAL) parameters. A blood sample was collected and tested for blood grouping test, and a saliva sample was analyzed for secretion using hemagglutination inhibition test. Secretor patients were 82% compared with 64% of healthy control. Mean score of BOB and PPD and the clinical magnitude of CAL parameters were increased within secretors. The development of periodontal diseases increased among secretors by 2.61 times more than non-secretors (OR = 2.61, CI, 1.382–4.914, p = 0.004). Significant periodontal complaints associated with secretion are Bleeding gums (OR = 1.95, CI, 1.011–3.759, p = 0.049), malaligned teeth (OR = 4.49 (CI, 1.842–10.958), p = 0.0001), gingival recession (OR = 2.48, CI, 1.322–4.648, p = 0.007), and teeth decay (OR = 1.86, CI, 1.006–3.438, p = 0.05). Secretion of ABH antigens is risk factor for progression of periodontal diseases. The odds of periodontal complaints, bleeding gums, malaligned teeth, gingival recession, and tooth decay prove the ABH secretion effect.

Phenotypic and Allelic Frequency of ABO and Rh (D) Blood Group among Whole Blood Donors in a Teaching Hospital of Chhattisgarh

Background: ABO and Rh (D) blood groups are the most important in blood transfusion and are determined genetically. Although these blood groups are common to all humans, there is variation in their allelic frequency based on region and population. This study was performed to determine the allelic frequency of ABO & Rh (D) in the donor population in the Blood Center of Chhattisgarh located in Central India.
 Place and Duration of Study: It is a cross-sectional study performed in the Department of Transfusion Medicine & Blood Bank of a teaching hospital from July 2021-February 2022.
 Methodology: Only the accepted whole blood donors were included. ABO & Rh (D) blood grouping was performed by conventional tube technique and their allelic frequency was determined. We studied 4078 whole blood donors out of which 4055 were males and 23 were females.
 Results: Phenotypic frequency of ABO blood group system was O>B>A>AB. Rh (D) positive was more prevalent than Rh (D) negative. Allele frequency of ABO system was 0.1545 for IA, 0.2351 for IB, and 0.6105 for IO. In Rh system, allele frequency of ID was 0.8441 and Id was 0.1559.
 Conclusion: Phenotypic & allelic frequency of ABO & Rh (D) shows heterogeneous distribution in different parts of the world. Our study showed blood group O & allele IO as the most common. This data is of utmost importance in the planning of transfusion services, especially during a healthcare crisis in low-resource area like ours.

The impact of blood group phenotypes on COVID-19 severity and mortality in Duhok province: a prospective cross-sectional study

Objectives: This study aimed to determine the frequency of blood group types among COVID-19 patients and to investigate its potential association with disease severity and patient outcomes.
 Methods: this prospective cross-sectional study was conducted from February to July 2022 in three different healthcare facilities in the Duhok region. All confirmed PCR confirmed COVID-19 patients were classified into: mild, moderate, severe, and critical cases. Information on demographic, clinical, and laboratory characteristics were collected using a standardized questionnaire. All patients were subjected to ABO blood grouping. The statistical calculations were performed by JMP Pro 14.3.0. A p value of < 0.05 was considered significant.
 Results: the study comprised of 404 patients. The age range of the patients varied from 16 to 100 years with a male predominance (204, 50.5%). Out of the total patients, 250 (61.88%) had mild-moderate course, while 154 (38.12%) had severe-critical course. The most frequent blood group was O (164, 41.58%), followed by blood group A (121, 29.95%). There was no significant difference in the distribution of the Rh factor among the studied subjects (p=0.426). There was a significant increase in disease severity and worse outcome with increasing age (p=<0.0001). Considering blood group types, there were no significant differences between blood group types with COVID-19 severity and patients’ outcome. 
 Conclusions: individuals with blood group O may have a higher risk of contracting COVID-19. Advanced age is a crucial predictor of disease severity and poor outcomes. There were no significant association between blood group types with COVID-19 severity and patients’ outcome. However, further research is needed to confirm these findings and determine the underlying mechanisms behind any potential association between blood groups and COVID-19 susceptibility, severity, and outcome.

Blood Group System: Clinical Insight and Relevance in Medical Practice

Purpose: Thirty three blood group systems have recently received recognition from the International Society of Blood Transfusion. Numerous types of antigens have been found on the red cell membranes in addition to the ABO and Rhesus systems. One of the few significant tests that the anesthesiologist orders during the perioperative period is blood grouping and cross matching. To avoid difficulties associated with transfusions, it is crucial to have a thorough awareness of the blood group system, their clinical importance, typing and cross-matching tests and current implications. To explore the various blood group systems, their clinical significance, and their relevance in medical practice, including transfusion medicine, prenatal care and genetic testing
 Methodology: Relevant databases such as PubMed, Google Scholar and Scopus to search for literature related to blood group system were used for this study. Search strategy including keywords related to barium studies, cross-sectional imaging and low resource economies. This may include terms such as blood group system, ABO blood group, clinical significance, medical practice. Search terms in combination with Boolean operators (AND, OR, NOT) are used to retrieve relevant articles.
 Findings: Undoubtedly blood group systems is highly relevant in medical practice so as to prevent haemolytic transfusion reaction and alloimmunization of individual blood which can result to death of infants and other individuals. As beneficial as blood transfusion is blood group system of the donor and recipient must be identified to make the blood transfusion process safe. Blood group systems also gives clinical insights in medical practice which make disease diagnosis and treatment easier to approach.
 Recommendations: Health care providers should be trained to properly identify and type blood samples and blood banks should have appropriate quality control measures in place to ensure the accuracy of blood typing and cross matching. Healthcare providers involved in organ transplantation should be familiar with the blood group compatibility guidelines and communicate effectively with blood banks to ensure the availability of compatible donor organs.

Study of the Frequency and Specificity of Red Cell Antibodies in Patients with Hemoglobinopathies

Patients with thalassemia and sickle cell disease (SCD) require blood transfusions as part of their supportive care. However, one of the most serious side effects of this treatment is the risk of red cell alloimmunization. The goal of this study was to assess the prevalence and Specificity of red cell alloimmunization in Egyptian thalassemia and sickle cell anaemia patients. This study included 200 multi transfused Egyptian patients, one hundred and forty patients with transfusion dependent thalassaemia and sixty patients with sickle cell anaemia, who were attending the Paediatric Children Hospital-Cairo University at the period from March 2019 to October 2019. Alloantibody identification was made by Diamed- ID microtyping system. In the studied groups both thalassemia and sickle patients, the prevalence of alloimmunization was 22/200 (11%) patients. The two most often alloantibodies were, antibodies against Kell antigen (37%) and against E antigen (30%). The prevalence of alloimmunization was more in females in comparison to males, but it did not reach statistical significance and patients with thalassemia major had higher alloimmunization rates than other studied groups but was not statistically significant. In the D negative patients in the research group, alloimmunization demonstrated a statistically significant difference (p = 0.01). Age, gender, age of transfusion onset and splenectomy were not contributing factors to the antibody presence in the group of patients being investigated. Before receiving blood transfusions, extended red blood cell phenotyping should be thought of as a crucial procedure for hemoglobinopathies patients who would likely have several transfusions. It is advised that haemoglobinopathies patients in Egypt be checked through phenotyping of RBC units for Kell and all Rh antigens to be phenotyped before starting transfusion in these patients which is also standard of care for these patients presently.

Assessment of Functioning of Health and Wellness Centers of Western Odisha: A Cross-Sectional Study.

Background The National Health Policy (NHP) 2017 of India recommends strengthening the delivery system of Primary Health Care through the establishment of Health & Wellness Centres (HWC) as the platform to deliver comprehensive primary health care services.HWCs are being set up as an upgraded version of existing sub-centers, primary health care centers, and urban primary health centers. This study was conducted to evaluate the functioning of health and wellness centers in Western Odisha. Objective To assess the availability of human resources, health care services, drug availability, lab services, and IT services at the health and wellness centers of Western Odisha. Methods Out of 10 districts of Western Odisha, two districts (Sambalpur and Deogarh) were selected for convenience, and a cross-sectional study was conducted from January 2021 to December 2022. All 43 health and wellness centers (35 rural primary health centers (PHCs) and eight urban PHCs) of the above two districts were included in the study. All relevant data were collected using a predesigned, pretested, and semi-structured questionnaire. Results The study showed that all 43 HWCs had good availability of pharmacists and lab technicians but less availability of medical officers, AYUSH medical officers, and staff nurses. Maternal and childhood services, family planning, and non-communicable disease (NCD) services were conducted regularly in all health and wellness centers, but basic oral health services and palliative care services were inadequate. All laboratory services like blood grouping, differential count/total leucocyte count, rapid test for pregnancy, urine albumin, urine routine examination/microscopic examination along with culture/sensitivity and water quality testing were done at urban PHC HWCs, whereas these lab services were less available at rural PHC HWCs.Drug groups like antipyretics, antihistaminic, antifungal, antihypertensive, oral hypoglycemic agent (OHA), antispasmodics, and antiseptic ointments were adequately available (>80%) at all urban and rural PHC HWCs. For IT support, appliances like desktops, internet facilities, and telephone facilitieswerefound to be present at all HWCs. Teleconsultation services were found to be available at 88% of urban PHC HWCs and 60% of rural PHC HWCs. ​Conclusion The study showed that infrastructure, human resources, and 12 service packages of health care and drugs should be addressed on a priority basis to achieve desired goals as envisaged by Ayushman Bharat to achieve the full potential of the health and wellness centers.

Frequency analysis of subgroup blood typing and Cis-AB type

The purpose of this study is to prevent side effects of transfusion by analyzing the frequency of subtype blood types in blood type tests. Of the 19,800 cases, 128 (0.59%) cases were classified as subtypes. The distribution of subtype detection in K University Hospital was classified as Cis-AB (39 cases, 30.5%), A₂ (25 cases, 21.6%), A₂B (24 cases, 20.7%), A₁B (13 cases, 11.2%), A₃ (11 cases, 9.5 cases) %), B₃(9 cases, 7.8%), Ag(3 cases, 2.6%), Ax(1 case, 0.95%), Ay(1 case, 0.9%), Bx(1 case, 0.9%), Bel(1 case, 0.9%). Am, Az, and Bm were not detected at all. These subtype blood types are caused by quantitative and qualitative variants of antigenicity on the surface of red blood cells. Finally, the examiner should select blood that matches the patient's blood type and select blood that is not abnormal in the examination and transfuse it to prevent side effects of transfusion in advance.

Training of medical students in the use of emergency whole blood collection and transfusion in the framework of a civilian walking blood.

In this report, we describe a training program in emergency whole blood collection and transfusion for medical students at the University of Bergen. The overall aim of the program is to improve the availability of early balanced blood transfusion for the treatment of patients with life-threatening bleeding in rural health care services. The voluntary training program provides the knowledge needed to practice emergency whole blood transfusions and understand the system for emergency whole blood collection in the framework of a civilian walking blood bank (WBB). It includes theoretical and practical sessions. In-person teaching and web-based learning resources are provided. An anonymous survey of the students attending the training course in the autumn of 2022 and spring 2023 was performed. 128 of 178 students participated in the practical training. 88 of 128 (69%) responded to the survey. 82 (93%) performed blood typing, 71 (81%) performed donor interviews, 61 (69%) partially performed whole blood collection (up to blood in bag) and 27 (30%) participated in complete whole blood collection and performed autologous reinfusion. No complications occurred during training. The students reported that the training course increased their understanding of how to ensure access to emergency blood transfusion by the use of a WBB. Structured theoretical and practical training in emergency whole blood collection and emergency transfusion is feasible and of interest to medical students. A multidisciplinary approach to student training in emergency whole blood collection and transfusion should be considered.

Serological Characteristics of Subtype A Caused by New A Allele Mutation and a Family Survey

In this study, the results of forward and reverse blood typing of a male patient diagnosed as bronchiectasis were inconsistent, which were type O and type A respectively. Multiple experiments including genotyping and sequencing and family investigation were carried out to determine the subtype of ABO blood group and explore the serological characteristics of this subtype. Standard serological techniques were used to conduct forward and reverse typing, reverse blood typing enhancement test, H antigen identification, absorption-elution test, salivary blood group substances test, and PCR-SSP method for ABO genotyping and exon 6 and 7 sequencing. The proband's blood group was type O by forward typing, but antigen A could be detected by absorption-elution test, anti-A1 could be detected by reverse blood typing enhancement test, it was found that there was substance H but no substance A in saliva, and the serological characteristics were consistent with Ael subtype. Gene sequencing analysis showed that there was a c.625T>G base substitution on the basis of A102, which had never been reported before. Family survey showed that c.625T>G base substitution appeared in three generations of the family. In this study, a new subtype A with Ael serological characteristics caused by c.625T>G mutation was identified. c.625T>G base substitution results in the weakening of A antigen, and this mutation can be stably passed down to future generations.

A COMPARISION OF CONVENTIONAL TUBE METHOD AND COLUMN AGGLUTINATION TECHNOLOGY IN AN IMMUNOHEMATOLOGY LABORATORY

Background: Rapid technological advancements in pretransfusion procedures in an immuno-hematological laboratory are a result of the increased focus on the quality and safety of blood products. Numerous automated pretransfusion testing platforms have been developed as a result of the inherent drawbacks of the gold standard conventional tube method. The invention of Column Agglutination Technology was the biggest advancement among these. Aim: 1. To compare the results of blood grouping and crossmatching by Conventional Tube Method and Column Agglutination Technology. 2. To statistically evaluate the results obtained between Column Agglutination Technology and Conventional Tube Technique in our immunohematology laboratory. Materials and Methods: The study was performed in blood bank of a tertiary hospital in Mangalore during the month of January,2022 in which 500 samples were subjected to blood grouping and cross matching. The data obtained was extensively studied. Sensitivity, specicity and statistical analysis were done by Fisher's exact test. Results: In comparative evaluation of CAT and CTT of 500 samples, there was concordance rate of 99.6% in blood grouping and concordance of 99.8% in crossmatching. In both the scenarios, the discordance was evaluated and found CATis superior to CTT. The most common blood group of both studies was 0 positive. Statistical analysis using Fisher's test proved statistically signicant difference between grouping and cross-matching via both tests with a p value of 0.000001 and 0.01 respectively. The sensitivity and specicity of both tests showed CAT to be superior with 100% sensitivity and 100% specicity while in CTT, the sensitivity was 100% with 66.6% specicity. Conclusion: The study establishes that CAT is more sensitive, specic, rapid, reliable procedure and is a better substitute of CTT in pretransfusion testing.

5613165 INVESTIGATING VARIANTS ASSOCIATED WITH QUANTITATIVE RED BLOOD CELL PHENOTYPES FROM TOPMED PREDICTIONS IN AFRICAN SICKLE CELL DISEASE POPULATIONS.

Background: Sickle cell disease (SCD) is caused by a single point mutation (c.20A>T, p.Glu6Val), which results in the formation of mutant haemoglobin S (HbS) instead of haemoglobin A (HbA). It is a life-long inherited recessive disorder that predominantly affects people of African, Indian, and Mediterranean descent. The polymerisation of HbS within red blood cells (RBCs) is the primary pathophysiological alteration in SCD, which results in the sickle-shape of RBCs. Sickle-shaped RBCs are more prone to haemolysis and blocking blood flow, resulting in anaemia and painful episodes. Haematological indices including haematocrit (HCT), haemoglobin concentration (Hb), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), red blood cell width (RDW) and RBC count indicate the size, development, and haemoglobin content of RBCs. Haematological indices are routinely measured as a means of monitoring SCD. A study recently found associations between 14 novel variants and haematological indices when using whole genome sequencing (WGS) data. The association between these variants and haematological indices have not yet been investigated in African SCD patients. This study aims to determine the associations between these variants and haematological indices in SCD patients from Cameroon and Senegal compared to non-SCD individuals. Aim: Investigating the association of 13 novel variants previously found in the Trans-Omics for Precision Medicine (TOPMed) project to haematological indices in African SCD patients. Methods: The 14 variants from the TOPMed study will be genotyped using a TaqMan Assay platform. Sanger sequencing will then be performed on 10% of the cohort to validate the TaqMan Assay result. The application R will be used for the descriptive statistics using sociodemographic, clinical, and haematological indices to evaluate the cases and controls. The Hardy-Weinberg Equilibrium will be used to ensure quality control. A linear regression analysis will be performed in R to determine the association between the variants and RBC phenotypes. Expected results: We are expecting to find an association between the SNPs and haematologocial indices. Conclusion: The variants of focus in this study were prioritized using WGS data, therefore capturing variants that are usually not included in GWAS arrays or imputation reference panels. The associations of the 13 novel variants with haematological indices have not yet been tested in African SCD populations. The results of this investigation could positively impact future studies into predictive modelling using haematological indices.

Extended red blood cell antigens and phenotypes in Burkina Faso: potential issues to design local population-sourced red blood cell reagent panels.

To date, 43 blood group systems with 349 red blood cell (RBC) antigens have been recognized. The study of their distribution is useful for blood services to improve their supply strategies for providing blood of rare phenotypes, but also to design indigenous RBC panels for alloantibody screening and identification. In Burkina Faso, the distribution of extended blood group antigens is not known. This study aimed to investigate the extended profiles of blood group antigens and phenotypes of this population and to raise limitations and potential strategies for the design of local RBC panels. We conducted a cross-sectional study that included group O blood donors. Extended phenotyping for antigens in the Rh, Kell, Kidd, Duffy, Lewis, MNS, and P1PK systems was performed using the conventional serologic tube technique. The prevalence of each antigen and phenotype combination was determined. A total of 763 blood donors were included. The majority were positive for D, c, e, and k and negative for Fya and Fyb. The prevalence of K, Fya, Fyb, and Cw was less than 5 percent. The most frequent Rh phenotype was Dce, and the most common probable haplotype was R0R0 (69.5%). For the other blood group systems, the K-k+ (99.4%), M+N+S+s- (43.4%), and Fy(a-b-) (98.8%) phenotypes were the most frequent. Antigenic polymorphism of blood group systems by ethnicity and geography argues for the design and evaluation of population-sourced RBC panels to meet specific antibody profiles. However, some of the specificities identified in our study, such as the rarity of double-dose antigen profiles for certain antigens and the cost of antigen phenotyping tests, are major challenges to overcome.

Effect of iron fortification on anaemia and risk of malaria among Ghanaian pre-school children with haemoglobinopathies and different ABO blood groups

BackgroundHaemoglobinopathies such as sickle cell disorder and glucose-6-phosphate dehydrogenase (G6PD) deficiency as well as differences in ABO blood groups have been shown to influence the risk of malaria and/or anaemia in malaria-endemic areas. This study assessed the effect of adding MNP containing iron to home-made weaning meals on anaemia and the risk of malaria in Ghanaian pre-school children with haemoglobinopathies and different ABO blood groups.MethodsThis study was a double-blind, randomly clustered trial conducted within six months among infants and young children aged 6 to 35 months in rural Ghana (775 clusters, n = 860). Participants were randomly selected into clusters to receive daily semiliquid home-prepared meals mixed with either micronutrient powder without iron (noniron group) or with iron (iron group; 12.5 mg of iron daily) for 5 months. Malaria infection was detected by microscopy, blood haemoglobin (Hb) levels were measured with a HemoCue Hb analyzer, the reversed ABO blood grouping microtube assay was performed, and genotyping was performed by PCR–RFLP analysis.ResultsThe prevalence of G6PD deficiency among the study participants was 11.2%. However, the prevalence of G6PD deficiency in hemizygous males (8.5%) was significantly higher than that in homozygous females (2.7%) (p = 0.005). The prevalence rates of sickle cell traits (HbAS and HbSC) and sickle cell disorder (HbSS) were 17.5% and 0.5%, respectively. Blood group O was dominant (41.4%), followed by blood group A (29.6%) and blood group B (23.3%), while blood group AB (5.7%) had the least frequency among the study participants. We observed that children on an iron supplement with HbAS had significantly moderate anaemia at the endline (EL) compared to the baseline level (BL) (p = 0.004). However, subjects with HbAS and HbAC and blood groups A and O in the iron group had a significantly increased number of malaria episodes at EL than at BL (p < 0.05). Furthermore, children in the iron group with HbSS (p < 0.001) and the noniron group with HbCC (p = 0.010) were significantly less likely to develop malaria.ConclusionsIron supplementation increased anaemia in children with HbAS genotypes and provided less protection against malaria in children with HbAC and AS and blood groups A and O.Trial registrationclinicaltrials.gov Identifier: NCT01001871. Registered 27/10/2009.Registration number: https://clinicaltrials.gov/ct2/show/record/NCT01001871.

Confined Blood Chimerism in Monochorionic Diamniotic Dizygotic Twin

Monochorionic diamniotic dizygotic (MCDADZ) twins are extremely rare. However, MCDADZ cases have been reported as in vitro fertilization-embryotransfer has become more common and genetic tests have become possible. In MCDADZ twin, vascular anastomosis occurs by sharing the placenta, then twin-twin transfusion syndrome or the confusion in blood typing or genetic test due to blood chimerism can occur. Authors report a case of MCDADZ twin who showed blood chimerism detected by karyotyping and chromosome microarray. They were born through in vitro fertilization, and confined blood chimerism was diagnosed by additional short tandem repeat study. Although there was no twin-twin transfusion syndrome or disconcordant blood-typing in our twins, genetic counseling and long-term follow up is needed for possible confusion in laboratory test due to persistent blood chimerism in the future.

Use of selective phenotyping and genotyping to identify rare blood donors in Canada

The distribution of rare and specific red cell phenotypes varies between races and ethnicities. Therefore, the most compatible red cell units for patients with haemoglobinopathies and other rare blood requirements are most likely to be found in donors from similar genetic backgrounds. Our blood service introduced a voluntary question asking donors to provide their racial background/ethnicity. Results triggered additional phenotyping and/or genotyping. We analysed the results of additional testing performed between January 2021 and June 2022, and rare donors were added to the Rare Blood Donor database. We determined the incidence of various rare phenotypes and blood group alleles based on donor race/ethnicity. Over 95% of donors answered the voluntary question; 715 samples were tested, and 25 donors were added to the Rare Blood Donor database, including five k-, four U-, two Jk(a-b-) and two D- - phenotypes. Asking donors about their race/ethnicity was well received by donors, and the resulting selective testing enabled us to identify individuals with a higher likelihood of being rare blood donors, support patients with rare blood requirements and better understand the incidence of common and rare alleles and red blood cell phenotypes in the Canadian donor population.

TAS2R38 gene in relation to Helicobacter pylori infection and blood groups in different age groups

Of the factors predisposing to gastric cancer is Helicobacter pylori infection affecting more than 50% of the general population. Genetic variation is an established player in certain diseases susceptibility. TAS2R38 gene polymorphisms have been found to influence bitter taste ability to chemicals with malicious characteristics and consequently affect metabolism and disease development. This study aimed to investigate the correlation between TAS2R38 gene polymorphisms and H. pylori seropositivity. The study involved 105 apparently healthy individuals. They were grouped into four groups according to their age and gender; young male, young female, middle-aged male and middle-aged female. All groups were tested for H. pylori serum antibody using screening rapid test. Participants were also tested for tasting PTC for TAS2R38 gene detection by using Bartovation PTC test paper and grouped accordingly into: homozygote (highly bitter taste), heterozygote (slight to moderate bitter taste), or negative gene carrier (no taste at all). ABO and Rhesus- blood grouping was determined by standard serological analysis. Of the 105 patients, 22.85% were tested homozygotes for TAS2R38 gene, 40.95% were heterozygotes and 36.19% were nontasters, no significant difference (p &amp;gt; 0.9). H. pylori seropositivity was encountered in 16.19% of the whole participants, 11.5% of the male participants and 20.75% of the female participants (p &amp;gt; 0.9). No significant difference in seropositivity was monitored among the four age groups (p &amp;gt; 0.3) and the ABO/Rh blood groups (p &amp;gt; 0.9). A lack of significant correlation (r = 0.046) between H. pylori antibody test positivity and tasting PTC (TAS2R38 gene) was reported. Similarly, no association was found between PTC tasting and participants’ ABO blood grouping, age or gender (r = 0.086, 0.083 and 0.029, respectively). Yet, weak negative (reverse) relationship (r = -0.29, p-value = 0.002) was gained between PTC and Rh grouping. No correlation was revealed between TAS2R38 polymorphism and the studied variable; age, gender and blood group indicating the absence of an apparent role of the gene in vulnerability to H. pylori infection. Further studies involving a larger sample size is required to confirm the obtained result.

Validation of a cage-side agglutination card for Dal blood typing in dogs.

Although 98% of the canine population is Dal-positive, Dal-negative dogs are more common in some breeds such as Doberman Pinschers (42.4%) and Dalmatians (11.7%), and finding compatible blood for these breeds may be challenging, given limited access to Dal blood typing. To validate a cage-side agglutination card for Dal blood typing and determine the lowest packed cell volume (PCV threshold) at which interpretation remains accurate. One-hundred fifty dogs, including 38 blood donors, 52 Doberman Pinschers, 23 Dalmatians and 37 anemic dogs. Three additional Dal-positive canine blood donors were included to establish the PCV threshold. Dal blood typing was performed on blood samples preserved in ethylenediaminetetraacetic acid (EDTA) <48 hours using the cage-side agglutination card and a gel column technique (gold standard). The PCV threshold was determined using plasma-diluted blood samples. All results were read by 2 observers, blinded to each other's interpretation and to the sample's origin. Interobserver agreement was 98% and 100% using the card and gel column assays, respectively. Overall, the sensitivity and specificity of the cards were 86%-87.6% and 96.6%-100%, respectively, depending on the observer. However, 18 samples were mistyped using the agglutination cards (15/18 by both observers): 1 false-positive (Doberman Pinscher), and 17 false-negative samples including 13 anemic dogs (PCV range, 5%-24%; median, 13%). The PCV threshold allowing reliable interpretation was determined to be >20%. Dal agglutination cards are reliable as a cage-side test, but results should be interpreted cautiously in severely anemic patients.

ABO Blood group Detection in Teeth from Cadaver: A Modified Absorption-Elution Technique

In a medicolegal investigation, a forensic pathologist, sometimes known as a forensic dentist, works hand in hand with a police officer to determine an individual's identification as a source of information. The blood group might be essential in establishing the victim's identification and the culprits who committed the crime in medico-legal investigations. Because dental tissues are hard of all human tissues and may be conserved for a long time even after death, they are significant for forensic purposes. The main goal of this study was to use the Adsorption-Elution method to detect blood group and Rhesus factor from soft and hard tissue of the tooth as biological evidence and also determine the ABO blood grouping from patients and cadavers in forensic investigation. The results showed, that there was a strong positive matching of the blood grouping with the coagulation of the tooth specimen.

Pengembangan Virtual Laboratory Biologi Pada Praktikum Uji Golongan Darah

This study aims to determine the results of the development, measure the feasibility and response of students from the Biology Virtual Laboratory in the Blood Type Test Practicum. Research and Development (R&D) is the method used in this study. With eight stages of the Sugiyono method, namely the first stage is the initial stages of potential and problems up to the final product. August-November 2021 the implementation of this research used 35 samples of class XI students. This study used a questionnaire instrument from 3 experts, namely material experts, media experts as well as field practitioners and 35 students. This study obtained the percentage results from material experts 93% with a very appropriate category, based on media experts 93% with a very feasible category, and field practitioners (Biology teachers) with a percentage of 92%. As for the results of the students' responses, they obtained a score of 90% with a very feasible category, so it can be concluded that the Biology Virtual Laboratory is suitable for use in practicum activities for blood type testing.

Analysis of Blood Group Parameters as an Early and Minimally Invasive Method for Susceptibility Prediction of Oral Squamous Cell Carcinoma

Aim: The purpose of this study was to evaluate the correlation between ABO blood grouping and oral squamous cell carcinoma and to stratify the patients in accordance with the susceptibility as noted.&#x0D; Materials and Methods: This retrospective study included the demographic data of the patients having reported to the Department of Oral and Maxillofacial Surgery, Yenepoya Dental College and hospital, and Department of Oncology, Yenepoya Medical college and Hospital, Mangalore, Karnataka between January 2017 to December 2021. A total of 161, histopathologically proven oral squamous cell carcinoma patients, between the above time frame of 5 years, were recruited and were analyzed for the study.&#x0D; Results: Patients with A Rh positive blood group had the maximum enrollment (80 patients out of 161) i.e. 49.6%, followed by an equal share of O Rh positive and B Rh positive with 31 each, i.e. 19.25% of each group. Only 8 patients had AB Rh positive blood group, followed by O Rh negative in 6 patients, A Rh negative in 3 patients, and least of B Rh negative blood group in only 2 patients.&#x0D; Conclusion: Our study reflects that as simple as a blood group test, a routine and a regularly followed investigation, can help to sought and stratify the patients, with oral squamous cell carcinoma, who already are on a higher spectrum of falling under the radar of malignancy susceptibility.