ABO Blood Research Articles

Hemoadsorption in Organ Preservation and Transplantation: A Narrative Review.

Cytokine adsorption can resolve different complications characteristic of transplantation medicine, such as cytokine storm activation and blood ABO and immune incompatibilities. Cytokine adsorption is also performed for the treatment of various life-threatening conditions, such as endotoxic septic shock, acute respiratory distress syndrome, and cardiogenic shock, all potentially leading to adverse clinical outcomes during transplantation. After surgery, dysmetabolism and stress response limit successful graft survival and can lead to primary or secondary graft dysfunction. In this clinical context, and given that a major problem in transplant medicine is that the demand for organs far exceeds the supply, a technological innovation such as a hemoadsorption system could greatly contribute to increasing the number of usable organ donors. The objectives of this review are to describe the specific advantages and disadvantages of the application of cytokine adsorption in the context of transplantation and examine, before and/or after organ transplantation, the benefits of the addition of a cytokine adsorption therapy protocol.

Association between ABO blood group/genotype and COVID-19 in a Japanese population.

An association between coronavirus disease 2019 (COVID-19) and the ABO blood group has been reported. However, such an association has not been studied in the Japanese population on a large scale. Little is known about the association between COVID-19 and ABO genotype. This study investigated the association between COVID-19 and ABO blood group/genotype in a large Japanese population. All Japanese patients diagnosed with COVID-19 were recruited through the Japan COVID-19 Task Force between February 2020 and October 2021. We conducted a retrospective cohort study involving 1790 Japanese COVID-19 patients whose DNA was used for a genome-wide association study. We compared the ABO blood group/genotype in a healthy population (n = 611, control) and COVID-19 patients and then analyzed their associations and clinical outcomes. Blood group A was significantly more prevalent (41.6% vs. 36.8%; P = 0.038), and group O was significantly less prevalent (26.2% vs. 30.8%; P = 0.028) in the COVID-19 group than in the control group. Moreover, genotype OO was significantly less common in the COVID-19 group. Furthermore, blood group AB was identified as an independent risk factor for most severe diseases compared with blood group O [aOR (95% CI) = 1.84 (1.00-3.37)]. In ABO genotype analysis, only genotype AB was an independent risk factor for most severe diseases compared with genotype OO. Blood group O is protective, whereas group A is associated with the risk of infection. Moreover, blood group AB is associated with the risk of the "most" severe disease.

Distribution of ABO And Rh Blood Groups in Blood Donors at the Tertiary Care Centre, Dahod

Introduction When it comes to blood transfusion and transplant medicine, the ABO as well as Rh blood group system are significant. There have been 700 RBC antigens found so far, and they've been divided into 30 blood groups as a result. For example, blood transfusion reaction, paternity testing, legal medicine, as well as associations with different illnesses are all medicolegally relevant when looking at ABO and Rh. Aim: The objective was to find out how much demand there is for a specific blood type compared to how much supply there is in society, to lower death rates caused by a shortage of that kind of blood. Compare the results with those from another comparable research performed in India and across the globe. Material and Methods: Between August 2018 to July 2021, researchers conducted a study on 7820 blood donors who visited the Zydus blood Centre. Tube agglutination testing and the Gel card method were used to find the patient's ABO as well as Rh blood groups. Both reverse as well as forward blood grouping methods are performed and confirmed only if both are identical. The age and sex groups as well as frequency of ABO and Rh blood groups were reported in percentages. Result The male-to-female ratio in our research was 30:1. The greatest number of male and female donors were between the ages of 18 to 30 years. For blood donation, it has been found that "O" blood donors are the most predominant, then those with B>A>AB blood groups. According to voluntary and replacement blood donors, AB & A blood groups are more (%) in replacement blood donors as compare to O and B blood groups. Voluntary donors are 33.67% and replacement donors were 66.64%. Distribution of Rh+ve and Rh-ve blood groups in our study was 98.78% and 1.22% respectively. Conclusion It is essential to have a thorough understanding of the distinct blood groups distribution for ensuring that blood as well as its components are always available to recipients in a particular area. The present work will provide insight on a deficiency of particular blood groups, in a particular area, like A and AB blood group are more in replacement donor showing deficiency of that blood groups in this area in our study.

Thumb print patterns in relation to gender and blood groups: A study in central district of Tamil Nadu

In the presentexamination, an endeavor has been made to study the fingerprint patterns in Central district of Tamil Nadu population. Thumb print patterns in relation with gender and blood group were studied in 275 participants,134 males and 141 females among the age group 18-25. The present study was conducted at the department of biotechnology, V.S.B. Engineering College, Karur district during the year 2022. Fingerprint patterns were studied in left and right thumb among males & females and analyzed with ABO blood grouping.The outcome demonstrated that the predominant pattern among both male and female was the loop, trailed by whorl and arches.

An Insight Into the Distribution of Allele Frequency of ABO and Rh (D) Blood Grouping System Among Blood Donors in a Tertiary Care Hospital in Chengalpattu District of South India.

IntroductionThe distribution of ABO and Rh (D) blood groups and their allele frequencies vary from one population to another worldwide. The objective of the study is to estimate the distribution of ABO & Rh (D) blood groups among all the blood donors in a tertiary care hospital in Chengalpattu district of Tamilnadu in South India and to determine their allele frequencies.MethodsThis was a retrospective observational study carried out in the blood bank of Karpaga Vinayaga Institute of Medical Sciences and Research Centre from January 2015 to December 2021. ABO and Rh (D) blood grouping of all the blood donors were carried out by tube agglutination method. Allele frequency of the blood group genes was calculated based on Hardy-Weinberg equilibrium.ResultsOut of total a of 7598 blood donors, 7576 (99.71%) were males and 22 (0.29%) were females. The most common blood group was O positive (37.67%) while AB negative (0.18%) was the least common blood group. The phenotypic frequency of blood group O (39.17%) was the highest and that of blood group AB (7.88%) was the least. A majority (95.96%) of the blood donors were Rh (D) positive. The allele frequencies of ABO and Rh (D) blood groups were 0.1628 for IA, 0.2177 for IB, 0.6259 for IO, 0.7991 for ID, and 0.2009 for Id.ConclusionsThe distribution of the two major blood group systems namely ABO and Rh (D) systems show considerable heterogeneity in different populations of the world. Information about allele frequencies of blood groups among different populations worldwide will help in framing policy decisions to face future challenges in healthcare services.

Influence of Coronavirus Disease 2019 on some Physiological Blood Parameters in Patients with Kidney Failure in Iraq.

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by a novel coronavirus (Severe Acute Respiratory Syndrome Coronavirus 2; SARS-CoV-2), which is related to the SARS-CoV-2 and the Middle East Respiratory Syndrome Coronavirus, which caused serious outbreaks in 2003 and 2012. This study aimed to determine if there is an association between ABO blood types/renal failure and infection with COVID-19. Furthermore, the effects of COVID-19 infection on some blood parameters and electrolyte levels were investigated in this study. In the current study, 90 samples were obtained from males and females aged between 21-68 years old. The data were collected from September to February 2021 in a Kidney Center of Alsaader Teaching Hospital. The participants were divided into three groups (n=30) of A) kidney failure, B) kidney failure with COVID-19, and C) kidney failure with COVID-19 recovery after one month. The variables of this study included blood group types, blood electrolytes, and some blood biochemical parameters. According to the results, regarding the frequency of blood groups, in the control group, 34, 20, 14, and 36 participants belonged to the A , B, AB, and O blood groups, respectively. The recorded data showed that participants who had suffered from kidney failure and were infected with COVID-19 belonged to the A, B, AB, and O blood groups (25%, 10%, 27%, and 45%), respectively, while kidney failure patients who had recovered after one month from COVID-19 had blood groups of A, B, AB, and O (25%, 22%, 105%, and 45%, respectively). The recorded data showed a significant decrease (<i>P</i>&lt;0.05) in the levels of Potassium (K), Sodium (Na), and Calcium (Ca) in the B group, compared to the A group, while the levels of K, Na, and Ca had significantly improved in group C (<i>P</i>&lt;0.05), compared to group B. The Chloride level showed no significant differences among the groups. Furthermore, non-significant differences (<i>P</i>&gt;0.05) were observed in the red blood cells (RBC), hemoglobin (Hb), and white blood cell count (WBC) in the COVID-19 group (Group B), compared to group A; however, there was a significant raise (<i>P</i>&lt;0.05) in WBC and platelet (PLT), as well as a significant decrease (<i>P</i>&lt;0.05) in lymphocyte (LYM), RBC, Hb, and hematocrit (HCT) in group C, compared to groups A and B. In conclusion, blood group O obtained the lowest level of resistance to COVID-19, compared to blood group A which had the highest response to recovery. The COVID-19 patients with kidney failure showed a significant decrease in blood parameters, such as RBCs, Hb, LYM, PLT, HCT, and electrolytes.

Frequency and distribution of ABO and Rhesus (D) blood groups: A tertiary care hospital experience

Frequency and distribution of ABO and Rhesus (D) blood groups: A tertiary care hospital experience

Distribution pattern of ABO and Rh (D) blood group system among 1st-year medical students

Distribution pattern of ABO and Rh (D) blood group system among 1st-year medical students

The Correlation between Impacted Third Molar and Blood Group.

Background Blood type is among the most important genetical characteristics of any individual and is shown to be correlated with the development of a variety of illnesses including dental diseases. Finding the association of ABO and Rh blood groups and impacted tooth is important in order to predict which population is more vulnerable to grow impacted third molars that could lead to making better intervention. The present investigation tried to take a small step in that regard, by evaluating the correlation between ABO and Rh blood groups and the most commonly impacted tooth, bony impacted third molars among Iranian individuals. Methods The investigation was done retrospectively on patients who were referred to the Department of Oral Radiology, Shiraz University of Medical Science, Shiraz, Iran. The patients were classified according to their blood groups, and 40 patients were randomly selected for each blood type. Therefore, the impaction of their third molars was evaluated, and statistical analyses were done in order to find any association. Results A total of 320 participants contributed to the study; 136 (42%) were males and 184 (57%) were females. The mean age was 29 ± 6 years. Among all participants, 205 (64%) had no impacted third molar, 26 (8%) had one impacted third molar, 43 (13%) had 2 impacted third molars, 5 (1%) had 3 impacted third molars, and 41 (12%) had 4 impacted molars. Conclusion According to the results of the present study, nearly one out of three individuals has at least an impacted third molar in the Iranian population, being more prevalent in individuals between 20 and 30 years old. The evaluation of the relationship between the blood group and impacted third molar revealed that blood groups have no association with the impacted third molars. However, more studies with higher and diversified participants should be done to find comprehensive results.

Associations between smoking and blood-group, and the risk of dyslipidaemia amongst French women

Dyslipidaemia is a major risk factor for cardio-vascular disease, as it promotes atherosclerosis. While cross-sectional studies have identified higher serum cholesterol amongst individuals with the A blood group, there is less evidence from prospective studies whether this translates into a higher risk of dyslipidaemia that requires treatment, nor if this genetic factor interacts with smoking status. This study aimed to prospectively determine potential associations between smoking, ABO blood groups, and risk of incident dyslipidaemia requiring treatment, and to assess associations over strata of blood ABO group. We assessed associations between blood ABO group, smoking and dyslipidaemia in 74,206 women participating in the E3N cohort. We included women who did not have cardiovascular disease at baseline. Logistic regression was used to determine associations between ABO group, smoking and prevalent dyslipidaemia at baseline. Cox proportional hazard models were then used to determine if blood ABO group and smoking were associated with the risk of incident dyslipidaemia, amongst women free of dyslipidaemia at baseline. At baseline 28,281 women with prevalent dyslipidaemia were identified. Compared to the O-blood group, the non-O blood group was associated higher odds of with prevalent dyslipidaemia (ORnon-O = 1.09 [1.06: 1.13]). Amongst the women free of dyslipidaemia at baseline, 6041 incident cases of treated dyslipidaemia were identified during 454,951 person-years of follow-up. The non-O blood groups were associated with an increased risk of dyslipidaemia when compared to the O-group (HRnon-O = 1.16 [1.11: 1.22]), specifically the A blood-group (HRA = 1.18 [1.12: 1.25]). Current smokers were associated with an increased risk of incident dyslipidaemia (HR smokers = 1.27 [1.16: 1.37]), compared to never-smokers. No evidence for effect modification between smoking and ABO blood group was observed (p-effect modification = 0.45), although the highest risk was observed among AB blood group women who smoked (HR = 1.76 [1.22: 2.55]). In conclusion, the non-O blood groups, specifically the A group were associated with an increased risk of dyslipidaemia. Current smokers were associated with a 30% increased risk of dyslipidaemia. These results could aid in personalised approaches to the prevention of cardiovascular risk-factors.

Prevalence of ABO, Rh and KELL Blood Group Types and Transfusion- Transmissible Infections (TTI) among Blood Donors in Najran City, Saudi Arabia

The knowledge of Red blood cells polymorphism and blood group antigens prevalence at the local and regional levels is necessary for safe blood transfusion services. This study was aimed to estimate the prevalence of significant blood group phenotypes like ABO, Rh, and Kell among the Najran people of Saudi Arabia. The transfusion transmittable infection (TTI) rate and blood abnormalities among various blood types were assessed to ensure safe blood transfusion. ABO and Rh blood prevalence (n=970) and Rh phenotype polymorphism were estimated in over 531 unrelated donors. The blood samples were screened for certain TTIs like AHBC- Anti-hepatitis B core, HTLV-1- human T-lymphotropic virus type I, HCV- hepatitis C virus, HBsAg- Hepatitis B antigen, HIV- Human immunodeficiency virus, SIC- Sickle cell, MP- Malaria parasite, and SYP- Syphilis. The selected samples were also observed for blood abnormalities by performing a complete blood count (CBC). Out of 970 subjects, 966 were males, and only 4 were females. The O&gt;A&gt;B&gt;AB blood groups were identified with 46.89, 29.3, 9.1, and 2.38% prevalence among Rh-positive phenotype. While in the Rh system, 87.6% and 12.3% of Rh positive and Rh negative was observed. Among 953 samples, the prevalence of seropositive donors was approximately 5.66%. The screening showed about 5.036, 0.104, 0.314, 0.209, 2.18, 0.104, and 0.209% positivity for AHBC, HCV, HBsAg, HIV, SIC, MP, and SYP respectively. Results found that the frequency of D, C, E, c, and e were 99.9%, 67.98, 25.8, 77.9, 98.49%, respectively, in over 531 subjects. The e allele was more prevalent in Najran city. After observing the variations in the CBC parameters among the donors, it was perceived that about 28.78, 99.9, 29.41, and 31.6% of blood abnormalities were noticed for O, AB, B, and A blood groups, respectively. For blood banks and transfusion services, which play a significant role in the medical care of the patient, awareness of the distribution of the blood group is essential. Increasing consistency of blood donation programs would improve both donor satisfaction and motivation for potential donations of blood in near future.

Identifi cation of ABO, RH and KEL blood group antigens with serology and genotyping methods

Introduction. Blood transfusion is a strong practice in traumatology, internal medicine, haematology, obstetrics and transplantation, which demands safety of haemotransfusion with estimating the red blood cell group antigens in donor and recipient blood. Routine immunotyping techniques usually provide for an antigen identification to weak subgroups, albeit with certain inherent limitations of serology tests that can be overcome in a genotyping approach.Aim — performance assessment of serology and genotyping methods in the ABO, RH and KEL blood group identification.Materials and methods. A total of 55,489 donor and 1,898 patient blood samples have been analysed. Ambiguous cases of chimerism, panagglutination and inconsistent results were tackled with genotyping. Serology tests were performed with gel cards. Whole blood DNA extraction was performed with Qiagen chemistry. Allele-specific PCR was used for the erythrocyte ABO, RH and KEL antigen genotyping with BAG Diagnostics commercial kits and a 2% agarose gel product detection. Sanger sequencing was used to complement genotyping.Results. A combined use of serology tests and genotyping allowed a successful erythrocyte antigen-based blood group and Rh-status assignment in 26 donors and patients with ambiguous blood typing.Conclusion. Genotyping coupled with serologic methods can be advised in a hampered blood group identification.

Association of blood group with severity of dengue in a tertiary care hospital

Background: The dengue viruses belong to the family of Flaviviridae family and are arboviruses. One of the genetic factors influencing the severity is HLA antigen and ABO blood group.Methods: This was a prospective study conducted during July 2018 to May 2019 in the department of pediatrics of KIMS hospital, Bengaluru. Children between 0-18 years of age were included. The dengue serology, hemogram and blood group of these patients was investigated and compared with the severity of illness.Results: It was found that the distribution of blood groups among cases and controls were similar. However blood group O was slightly more susceptible to dengue infection (54%) than its distribution in general population (41%). In patients with primary infection, blood group O presented with milder forms of dengue (52.43%) as compared to distribution in general population (41.46%). A higher percentage of AB blood group presented as severe forms of dengue (11.63%) as compared with general population (7.32%). In patients with secondary infection, similar findings were observed. Interestingly, no cases of AB blood group presenting with secondary dengue had only mild symptoms and always presented with warning signs (0% incidence) or severe dengue.Conclusions: Dengue infection is prevalent in most parts of India with severe forms having high mortality. This study concludes that although the incidence of dengue fever is higher in children with blood group O, AB blood group is associated with severe forms of dengue, especially in secondary infections.

Ethnicity and outcomes in patients hospitalised with COVID-19 infection in East London: an observational cohort study

ObjectiveTo describe outcomes within different ethnic groups of a cohort of hospitalised patients with confirmed COVID-19 infection. To quantify and describe the impact of a number of prognostic factors, including...

ABO and Rh blood groups distribution in Aleppo Province, Syria

ABO and Rh blood groups distribution in Aleppo Province, Syria

Prevalence of Rhesus Negative Status Amongst Antenatal Atendees in the University of Uyo Teaching Hospital, Uyo, Nigeria: A 5-Year Review

Introduction: The Rhesus (Rh) antigen is the most polymorphic of human blood group systems second only to the ABO in transfusion and transplantation medicine with significance in iso-immunization which can cause haemolytic disease of the foetus and newborn. This study was carried out to determine the prevalence of Rhesus negativity amongst antenatal clinic attendees in the University of Uyo Teaching Hospital. Methodology: This was a 5-year retrospective study carried out between March 1st, 2012 and February 28th, 2017. Data containing the ABO and Rhesus blood group of all antenatal clinic attendees was collected from the Laboratory registers and analysed using Microsoft excel. Results: The prevalence rate of Rhesus D negative women was 4.40%. The average yearly attendance was 2861 women. The commonest blood group was O with 8232 (57.54%), followed by blood group A, 3416 (23.88%) and blood group B, 2388 (16.69%) while the least common blood group was AB, 271 (1.89%). Most Rhesus D-negative women were of blood group O; 382 (60.78%), followed by group A; 142 (22.58%), and blood group B was 94 (14.49%). AB blood group was the least; 11 (1.70%). Sensitization rate among the 629 Rhesus D-negative women was 1.11%. Conclusion: The prevalence of rhesus negativity is low in the population studied with an ABO distribution that mirrored the antenatal population, hence the overall need for immunoprophylaxis. It is however very necessary to educate and counsel all non-sensitized Rhesus negative women on the need for antenatal and post-partum immuno-prophylaxis as well as public enlightenment on the rationale for Rhesus typing, especially among women.

Influence of ABO and Rh Blood Groups on COVID-19 Outcomes

A range of studies have suggested that blood group and risk for severe COVID-19 are linked. Using a national database between 2007 and 2019 in Ontario,

Unique repertoire of anti-carbohydrate antibodies in individual human serum

Humoral immunity to pathogens and other environmental challenges is paramount to maintain normal health, and individuals lacking or unable to make antibodies are at risk. Recent studies indicate that many human protective antibodies are against carbohydrate antigens; however, little is known about repertoires and individual variation of anti-carbohydrate antibodies in healthy individuals. Here we analyzed anti-carbohydrate antibody repertoires (ACARs) of 105 healthy individual adult donors, aged 20–60+ from different ethnic backgrounds to explore variations in antibodies, as defined by binding to glycan microarrays and by affinity purification. Using microarrays that contained > 1,000 glycans, including antigens from animal cells and microbes, we profiled the IgG and IgM ACARs from all donors. Each donor expressed many ACAs, but had a relatively unique ACAR, which included unanticipated antibodies to carbohydrate antigens not well studied, such as chitin oligosaccharides, Forssman-related antigens, globo-type antigens, and bacterial glycans. We also saw some expected antibodies to ABO(H) blood group and α-Gal-type antigens, although these also varied among individuals. Analysis suggests differences in ACARs are associated with ethnicity and age. Thus, each individual ACAR is relatively unique, suggesting that individualized information could be useful in precision medicine for predicting and monitoring immune health and resistance to disease.

English

English

The distribution of the ABO and RH blood groups among different populations in the MENA region: A review

ABO and Rh (rhesus) blood grouping is one of the most widely available laboratory tests that could prevent possibly deadly mismatches following blood transfusion and organ transplantation. The distribution of the blood group antigens varies between different populations and ethnic groups. Our objective was to showcase these variations within the Middle East and North Africa (MENA) region and to outline the relationship between blood type and disease risk association to determine the current findings and outline possible future study areas. In healthy blood donors, blood group O was found to consistently be the most prevalent blood group and AB the least prevalent blood group except in Turkey where A is the most prevalent and, in the United Arab Emirates (UAE), where B is the least prevalent blood type.