The human eye is an imperfect refractive system which not only has defocus and astigmatism, but also has spherical aberration, coma and anomalistic high-order aberrations, all of which have certain influence on the imaging quality of retina. What's worse, aberration is further enlarged as a result of mydriasis in dark field and weak light, thus making the vision performance of human eyes far below diffraction limitation. Further research revealed that human eye visual imaging is not only connected with refractive system, but also is closely related to the subjective judgment of human brain and the process of neural system. In order to overcome the deficiencies, wave-front aberration measurement method and system that has subjective visual compensation is proposed and conducted in combination with objective measurement, which ensures more accurate and realistic measuring results. The experimental data revealed that wave-front aberration obtained from subjective visual compensation measuring method is smaller than objective measurement, which is the result of subjects’ adaptive correction when watching sighting targets. In addition, when subjects are watching different sighting targets, the fluctuation value of wavefront aberration is small. Therefore, it is concluded that subjective visual compensation measuring method contributes to aberration measuring improvement and obtains results match with the realistic state by taking into consideration the actual condition of human eyes when watching targets. Hopefully, these discoveries will be of positive and beneficial value to the determination of human eye aberration treatment.