8th Day Of Cycle Research Articles

Effect of adding human chorionic gonadotropin to frozen thawed embryo transfer cycles with history of thin endometrium

Embryo implantation process is a complex phenomenon and depends on fetal and maternal factors interaction. Endometrial thickness is needed for successful implantation. We designed this study in order to assess adding human chorionic gonadotropin (HCG) to the conventional protocol in endometrial preparation in women with thin endometrium and a history of in vitro fertilization-embryo transfer (IVF-ET) failure. The non-randomized clinical trial study (quasi experimental design) was performed on 28 patients. Participants were women who were candidate for frozen-thawed (ET) and had two previous failed ET cycles because of thin endometrial. HCG was administrated (150 IU, intramuscular) from the 8th day of cycle and when endometrial thickness reached at least 7mm HCG was discontinued and frozen thawed ET was done. Totally 28 patients were included. The mean ± SD age of participants was 30.39±4.7. The mean of endometrium thickness before and after HCG were 5.07±0.43 and 7.85±0.52, respectively p<0.001. Also, there were five clinically and chemically pregnant women. The findings of the study suggested that adding HCG to the conventional preparation method was an effective protocol and significantly improved endometrial thickness and pregnancy outcomes in women with previous embryo transfer failure because of thin endometrium.

Exogenous luteinizing hormone for assisted reproduction techniques in poor response patients

Two gonadotrophins, two cell theory refers to necessity of both gonadotrophin activities for theca and granulose cells steroidogenesis of dominant follicle. The aim of this study was to determine the influence of recombinant LH in women qualified as poor responders in the first assisted reproduction procedure (IVF), on fertility results, expressed as percentage of clinical pregnancies. The study included 12 women, 35 years and older who were their own controls. The next trial of IVF was with the same dose of recombinant FSH and GnRH agonist, and with the same, long protocol. Recombinant LH was added in the dose of 75 IU from the 2nd to 7th day of the cycle, and 150 IU from the 8th day of the cycle to the aspiration of oocytes. Within the two different protocols: there was no significant difference between LH concentration in 8th and 12th day of cycle; there was no significant difference between E2 concentration on day 2nd and day 8th; there was a significant difference between E2 concentrations on day 12th; endometrial thickness was not significantly different on the day of aspiration, neither was the number of follicles and embryos. In the two patients, clinical pregnancy was detected (pregnancy rate 17%), and they delivered in term. So, a statistically significant difference between the two protocols was in the rate of clinical pregnancies. The patients with low response to a long protocol in IVF procedures had significantly better results according to the clinical pregnancy rate when the recombinant LH was added to recombinant FSH in the stimulation protocol.

Steroid hormones and prolactin in porcine follicular fluid in estrous cycle and early pregnancy.

Concentrations of progesterone (P4), estradiol 17-beta (E2), testosterone (T) and prolactin (PRL) were estimated by RIA in porcine follicular fluid (FF) on 5th, 8th, 13th, 17th and 20th day of the estrous cycle and on 18th and 28th day of pregnancy. The highest P4 and E2 concentrations were found on 20th day of the estrous cycle. The P4 level measured on that day differed (P less than 0.05) from the levels on 5th, 13th and 17th day and E2 level on 20th day was different from the levels on remaining days of the estrous cycle, i.e. 5th, 8th, 13th and 17th. In pregnancy, concentrations of these steroids were much lower (P less than 0.05) compared to 20th day of the estrous cycle. No differences were found in T levels neither in estrous cycle nor in pregnancy. PRL concentration was at the lowest level on 5th day of the estrous cycle and it was different (P less than 0.05) from the concentrations on 8th day of the cycle and on 18th day of pregnancy (P less than 0.05). PRL content found on 28th day of pregnancy was lower (P less than 0.05) than on 18th day.