Reducing Pt loading in proton exchange membrane fuel cells (PEMFCs) with improved performance is highly demanded for their wide adoption.Herein, asymmetric Pt nanoparticles (NPs) onto 3D graphene porous film (3D-GF) as integrated electrode was developed for ultra-low Pt loading PEMFCs. The 3D-GF featuring highly graphitic degree and rich macro-porosity as the framework of constructing catalyst layer was prepared by arc-discharge method and Pt NPs were grown onto 3D-GF via atomic layer deposition. The Pt NPs prefer to grow along the surface of graphene, presenting unique characteristics of high lattice strain with extended contact interface with 3D-GF. The specific structure is completely distinguished from Pt based NPs with symmetric structures synthesized by wet chemical methods. The cell with Pt/3D-GF at 100 μgPt cm−2 as the cathode exhibits outstanding activity and durability, in which its maximum power density (MPD) is 2.07 W cm−2 and 0.88 W cm−2 under H2/O2 and H2/Air at 150.0 kPa respectively, much higher than that of the cell with Pt/C at 200 μgPt cm−2, as the mass activity (MA) of Pt/3D-GF is 3.0 times higher than that of commercial Pt/C catalysts, and its performance suffers from the MPD loss of 25 % and 7.6 % after accelerated durability tests (ADTs) with 30 K cycles at 0.6–0.95 V and 5 K cycles at 1.0–1.5 V respectively, which is also significantly lower than that of commercial Pt/C catalyst. The asymmetric Pt NPs on 3D-GF provides a new concept for achieving ultra-low Pt loading PEMFCs.