Optical signal processing is one of essential technologies for improving the flexibility of all-optical network. Above all, recently there have been a lot of studies regarding all-optical 2R/3R regeneration technology. However, there are few studies about all-optical 2R/3R technologies that are carried out in field environment. In this paper, we report the successful results of field trials of an all-optical 2R regeneration system based on an electro-absorption modulator for 40-Gbit/s WDM transmission systems with optical add/drop multiplexing. It was made sure that by applying the all-optical 2R regeneration system to the optical add/drop multiplexer in the 320-km-long transmission systems the transmission characteristics of the express signal after 320-km transmission and those of the dropped signal at 160-km can be made nearly the same. It is quite important that the transmission characteristics are equal for both the dropped and express channel from a point of view of the system design, and the results in this paper suggests one possible solution for this matter.