24-h Temperature Research Articles

Bond behaviour of ribbed steel bar and concrete at 24-h elevated temperatures: Experimental study and theoretical analysis

This study attempts to remedy the lack of understanding on the bond behaviour of steel bar in concrete at elevated temperatures in current research. Pull-out tests were conducted using a unique test setup, and the effects of temperature (120 °C – 350 °C), heating duration (3 h – 24 h), and anchorage length (105 mm – 175 mm) were given attention. In addition, calculation theories for bond behaviour at elevated temperatures were derived. All specimens eventually showed failure characterised by pull-out-splitting. The increase in temperature induces a continuous decrease in bond strength and a continuous increase in peak slip. Moreover, the bonding performance deteriorates gradually with the heating duration, but could be stabilised at 24 h. This can be interpreted as a sufficiently long heating duration allowing full development of concrete thermal damage. Under the same heating regime, the specimens with longer anchorage lengths show lower bond strength, however, the degree of change in bond strength with temperature is not significantly related to anchorage length. The specimens with anchorage lengths of 105 mm, 140 mm, and 175 mm respectively exhibit 15.4 %, 16.9 %, and 16.4 % reduction in bond strength, as the temperature increases from 120 °C to 350 °C. Increasing the temperature helps the bond stress to distribute more uniformly in the bonding zone. However, increasing the anchorage length intensifies the non-uniform distribution of bond stress and the stress peak becomes larger. The bond-slip constitutive model developed considering anchorage length is proved to accurately predict the bond behaviour of steel bar in concrete at elevated temperatures. For reinforced concrete structures working in thermal environments, 0.08 is suggested as ribbed steel bar shape factor.

Neuroprotective Effects of Inhaled Xenon Gas on Brain Structural Gray Matter Changes After Out-of-Hospital Cardiac Arrest Evaluated by Morphometric Analysis: A Substudy of the Randomized Xe-Hypotheca Trial.

We have earlier reported that inhaled xenon combined with hypothermia attenuates brain white matter injury in comatose survivors of out-of-hospital cardiac arrest (OHCA). A predefined secondary objective was to assess the effect of inhaled xenon on the structural changes in gray matter in comatose survivors after OHCA. Patients were randomly assigned to receive either inhaled xenon combined with target temperature management (33°C) for 24h (n = 55, xenon group) or target temperature management alone (n = 55, control group). A change of brain gray matter volume was assessed with a voxel-based morphometry evaluation of high-resolution structural brain magnetic resonance imaging (MRI) data with Statistical Parametric Mapping. Patients were scheduled to undergo the first MRI between 36 and 52h and a second MRI 10days after OHCA. Of the 110 randomly assigned patients in the Xe-Hypotheca trial, 66 patients completed both MRI scans. After all imaging-based exclusions, 21 patients in the control group and 24 patients in the xenon group had both scan 1 and scan 2 available for analyses with scans that fulfilled the quality criteria. Compared with the xenon group, the control group had a significant decrease in brain gray matter volume in several clusters in the second scan compared with the first. In a between-group analysis, significant reductions were found in the right amygdala/entorhinal cortex (p = 0.025), left amygdala (p = 0.043), left middle temporal gyrus (p = 0.042), left inferior temporal gyrus (p = 0.008), left parahippocampal gyrus (p = 0.042), left temporal pole (p = 0.042), and left cerebellar cortex (p = 0.005). In the remaining gray matter areas, there were no significant changes between the groups. In comatose survivors of OHCA, inhaled xenon combined with targeted temperature management preserved gray matter better than hypothermia alone. ClinicalTrials.gov: NCT00879892.

Effect of temperature-humidity index on the evolution of trade-offs between fertility and production in dairy cattle

BackgroundIn the current context of climate change, livestock production faces many challenges to improve the sustainability of systems. Dairy farming, in particular, must find ways to select animals that will be able to achieve sufficient overall production while maintaining their reproductive ability in environments with increasing temperatures. With future forecasted climate conditions in mind, this study used data from Holstein and Montbeliarde dairy cattle to: (1) estimate the genetic-by-temperature-humidity index (THI) interactions for female fertility, and (2) evaluate the production-fertility trade-off with increasing values of THI.ResultsTwo-trait random regression models were fitted for conception rate (fertility) and test-day protein yield (production). For fertility, genetic correlations between different THI values were generally above 0.75, suggesting weak genotype-by-THI interactions for conception rate in both breeds. However, the genetic correlations between the conception rate breeding values at the current average THI (THI = 50, corresponding to a 24-h average temperature of 8 °C at 50% relative humidity) and their slopes (i.e., potential reranking) for heat stress scenarios (THI > 70), were different for each breed. For Montbeliarde, this correlation tended to be positive (i.e., overall the best reproducers are less affected by heat stress), whereas for Holstein it was approximately zero. Finally, our results indicated a weak antagonism between production and fertility, although for Montbeliarde this antagonism intensified with increasing THI.ConclusionsWithin the range of weather conditions studied, increasing temperatures are not expected to exacerbate the fertility-production trade-off. However, our results indicated that the animals with the best breeding values for production today will be the most affected by temperature increases, both in terms of fertility and production. Nonetheless, these animals should remain among the most productive ones during heat waves. For Montbeliarde, the current selection program for fertility seems to be adequate for ensuring the adaptation of fertility traits to temperature increases, without adverse effects on production. Such a conclusion cannot be drawn for Holstein. In the future, the incorporation of a heat tolerance index into dairy cattle breeding programs would be valuable to promote the selection of animals adapted to future climate conditions.

Evaluation of blastocyst re-expansion, quality in relation to storage temperature, and sexing using blastocoel fluid after manual perforation with a hand-held needle involving in vivo produced equine embryos

The present study was designed to evaluate equine blastocyst re-expansion rate, quality, and sex following perforation of the blastocoel, collection of blastocoel fluid (BF), and PCR amplification of free DNA. Experiment 1 tested the feasibility of the BF sample collection with a hand-held, small-gauged needle (26g) and subsequent PCR amplification of the TSP-Y gene for males and AMEL-Y gene for males and AMEL-X gene for females. Experiment 2 tested the application of the technique. Equine embryos were collected via uterine flushes 8d after ovulation. Thereafter, embryos (n = 19) were initially assessed and transferred to a 50 μL droplet of holding medium in which the blastocoel was manually perforated as in Experiment 1. Within 1 min of detecting a diameter decrease or collapse, the entire volume of each droplet of medium was collected and stored at −20 °C until PCR. In Experiment 1, amplification of the TSP-Y gene was positive for males at 60% (9/15) and negative for females at 40% (6/15). In Experiment 2, a total of 42 embryos were randomly assigned to a collapsed embryo (CE) or intact embryo (IE) groups and stored at room temperature (RT, 25 °C) or cold temperature (CT, 5 °C) for 24h as follows: 1) CERT, n = 11; 2) CECT n = 11; 3) IERT, n = 10; and 4) IECT, n = 10. After 24h, embryo diameter and quality were reassessed. For all collapsed embryos (n = 19), blastocoel fluid was subjected to double PCR amplification of the TSPY gene with blood from adult male and female horses as controls. Positive gene amplification indicated 57.9% (11/19) of embryos were male and negative amplification indicated 31.6% (6/19) of embryos were female. Relative to the least diameter (0%) after perforation of collapsed embryos or fullest diameter (100%) of intact embryos at T0, percentage change in diameter and quality Grade 1 or 2 embryos after 24h of storage for all groups were, respectively: 31.2% and 54% for CERT group, 28.2% and 0% for CECT group, 25.9% and 100% for IERT group, 4.3% and 80% for IECT group, respectively. Thus, needle-induced leakage and collapse of the blastocoel at T0 resulted in a high rate of blastocyst re-expansion (69%) with many embryos (54%) achieving good quality at T24 with potential for transfer as either male or female embryos. For both collapsed and intact embryos, it was observed that storage for 24h at room temperature (25 °C) was associated with improved embryo growth and morphological quality compared to storage at cold temperature (5 °C).

Development of optic disc edema during 30 days of hypercapnic head-down tilt bed rest is associated with short sleep duration and blunted temperature amplitude.

Sleep and circadian temperature disturbances occur with spaceflight and may, in part, result from the chronically elevated carbon dioxide (CO2) levels on the international space station. Impaired sleep may contribute to decreased glymphatic clearance and, when combined with the chronic headward fluid shift during actual spaceflight or the spaceflight analog head-down tilt bed rest (HDTBR), may contribute to the development of optic disc edema. We determined if strict HDTBR combined with mildly elevated CO2 levels influenced sleep and core temperature and was associated with the development of optic disc edema. Healthy participants (5 females) aged 25-50 yr, underwent 30 days of strict 6° HDTBR with ambient Pco2 = 4 mmHg. Measures of sleep, 24-h core temperature, overnight transcutaneous CO2, and Frisén grade edema were made pre-HDTBR, on HDTBR days 4, 17, 28, and post-HDTBR days 4 and 10. During all HDTBR time points, sleep, core temperature, and overnight transcutaneous CO2 were not different than the pre-HDTBR measurements. However, independent of the HDTBR intervention, the odds ratios {mean [95% confidence interval (CI)]} for developing Frisén grade optic disc edema were statistically significant for each hour below the mean total sleep time (2.2 [1.1-4.4]) and stage 2 nonrapid eye movement (NREM) sleep (4.8 [1.3-18.6]), and above the mean for wake after sleep onset (3.6 [1.2-10.6]) and for each 0.1°C decrease in core temperature amplitude below the mean (4.0 [1.4-11.7]). These data suggest that optic disc edema occurring during HDTBR was more likely to occur in those with short sleep duration and/or blunted temperature amplitude.NEW & NOTEWORTHY We determined that sleep and 24-h core body temperature were unaltered by 30 days exposure to the spaceflight analog strict 6° head-down tilt bed rest (HDTBR) in a 0.5% CO2 environment. However, shorter sleep duration, greater wake after sleep onset, and lower core temperature amplitude present throughout the study were associated with the development of optic disc edema, a key finding of spaceflight-associated neuro-ocular syndrome.

Application of short-term analysis of skin temperature variability in prediction of survival in patients with cirrhosis.

Background: Liver cirrhosis is a complex disorder, involving several different organ systems and physiological network disruption. Various physiological markers have been developed for survival modelling in patients with cirrhosis. Reduction in heart rate variability and skin temperature variability have been shown to predict mortality in cirrhosis, with the potential to aid clinical prognostication. We have recently reported that short-term skin temperature variability analysis can predict survival independently of the severity of liver failure in cirrhosis. However, in previous reports, 24-h skin temperature recordings were used, which are often not feasible in the context of routine clinical practice. The purpose of this study was to determine the shortest length of time from 24-h proximal temperature recordings that can accurately and independently predict 12-month survival post-recording in patients with cirrhosis. Methods: Forty individuals diagnosed with cirrhosis participated in this study and wireless temperature sensors (iButtons) were used to record patients' proximal skin temperature. From 24-h temperature recordings, different length of recordings (30 min, 1, 2, 3 and 6h) were extracted sequentially for temperature variability analysis using the Extended Poincaré plot to quantify both short-term (SD1) and long-term (SD2) variability. These patients were then subsequently followed for a period of 12 months, during which data was gathered concerning any cases of mortality. Results: Cirrhosis was associated with significantly decreased proximal skin temperature fluctuations among individuals who did not survive, across all durations of daytime temperature recordings lasting 1hour or more. Survival analysis showcased 1-h daytime proximal skin temperature time-series to be significant predictors of survival in cirrhosis, whereby SD2, was found to be independent to the Model for End-Stage Liver Disease (MELD) score and thus, the extent of disease severity. As expected, longer durations of time-series were also predictors of mortality for the majority of the temperature variability indices. Conclusion: Crucially, this study suggests that 1-h proximal skin temperature recordings are sufficient in length to accurately predict 12-month survival in patients with cirrhosis, independent from current prognostic indicators used in the clinic such as MELD.

Evaluation of formaldehyde when complete feed and soybean meal were inoculated with porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus, and Seneca Valley virus 1.

Formaldehyde has been found to decrease virus concentrations in feed and ingredient matrices. Continued research is needed to identify the appropriate inclusion levels and application time for different viruses in these matrices. The objective was to evaluate different inclusion levels of formaldehyde when applied either pre- or postinoculation of porcine epidemic diarrhea virus (PEDV), type 2 porcine reproductive and respiratory syndrome virus (PRRSV), and Seneca Valley virus 1 (SVV1) to complete feed or soybean meal. The experiment was designed in a 2 × 2 factorial with a formaldehyde-based product (Termin-8, Anitox Corporation, Lawrenceville, GA) applied either before virus inoculation (preinoculation) or after inoculation (postinoculation) at either a 2 or 3kg/MT. On day 0, samples of the respective matrices were weighed in 50g aliquots and added to 500mL bottles. Formaldehyde was applied to the preinoculation samples at the respective inclusion levels and 50 µL of each virus were added to the postinoculation samples. All bottles were shaken and allowed to sit at room temperature for 24h. On day 1, virus was added to the preinoculation samples and formaldehyde was added to the postinoculation bottles. Half of the samples were immediately processed (0h) and the other half were incubated at room temperature for an additional 24h. Samples were processed and aliquots were analyzed via triplex PCR. An application time × inclusion level interaction was observed for PEDV at 0h and SVV1 and PEDV at 24h in complete feed, where less viral RNA (P < 0.05) was detected in the postinoculation samples at either inclusion level as compared to the positive controls. In soybean meal, the same interaction was observed in PEDV and PRRSV at 0h and SVV1 and PEDV at 24h with less detectable RNA observed (P < 0.05) in the postinoculation samples regardless of inclusion level than the preinoculation counterparts and the controls. Overall, an application time effect was noticed in each matrix where less RNA was detected in the postinoculation samples at 0h (P < 0.05) compared to the preinoculation samples and the control, and at 24h, both the pre- and postinoculation samples had less detectable RNA (P < 0.05) than the control. Overall, formaldehyde can reduce detectable RNA immediately in contaminated complete feed and soybean meal, with greater decreases observed as mitigant contact time increases.

Effects of land-use mitigation scenarios on urban heat island intensity in Istanbul

This is the first comprehensive study to examine how urbanization affects the microclimate of Istanbul using the urban climate model MUKLIMO_3 with a 200 m horizontal resolution. In this study, the parameters in the land use table were estimated for Istanbul by combining different land use data sets and creating a high-resolution land use distribution. A specified heat wave episode was simulated via MUKLIMO_3 by using defined land use, meteorological sounding, and surface measurements. The findings suggest that MUKLIMO_3 can reasonably capture the 24-h daily temperature variation in both rural and urban locations, but it has a negative bias compared to station observations. We also employ eight mitigation scenarios by changing the albedo of roofs and implementing green-roofs and walls. All scenarios reduced air temperatures to varying degrees in urbanized areas along the northern Marmara coastline on both sides of Istanbul during daylight hours. The white and hybrid scenarios resulted in a temperature drop of up to 2 K at 12:00 UTC, with the most significant cooling effect observed in hybrid 4. Increasing the albedo of roofs from 0.5 to 0.7 resulted in the spatial expansion of cooled areas towards the north of the city along the Bosphorus. However, green roof scenarios are not found to be as effective as high-albedo scenarios.

Biodiesel Production from Seed Oil Obtained from Pongamia pinnata L.

At the present time biodiesel are the most promising source of energy as it is renewable and can accomplished the rising energy demand. Pongamia pinnata L. (commonly known as Karanj) is an evergreen tree of family Fabaceae sub family Papilioneaceae, has been recognized as a potential source of biodiesel prepared from the oil obtained from its seeds. In the present work, biodiesel fuel was prepared by alkaline-catalyzed esterification of high free fatty acids present in Karanj oil. The transestrification reaction was performed by varying practical parameters such as NaOH percentage (0.50%, 0.75%, 1%), methanol to oil ratio ( 3:1, 6:1, 9:1), and reaction temperature and time (40°C for 2h, 60°C for 2h, room temperature for 24h) at constant stirring at 150 rpm. Optimum alkaline-catalyzed transesterification was achieved using 1% NaOH as a solid alkaline catalyst with a methanol-to-oil ratio of 1:6, temperature at 600C. The yield of methyl esters from Karanja oil under the optimal condition was 92%. The biodiesel and oil contents were analysed using Gas Chromatography and Mass Spectroscopy (GC-MS) analyser. Fuel properties of the biodiesel obtained by transesterification were tested and found to meet the ASTM (American Society for Testing and Material) specifications.

Cold and Hungry: Heterothermy Is Associated with Low Leptin Levels in a Bulk Grazer during a Drought.

AbstractReduced energy intake can compromise the ability of a mammal to maintain body temperature within a narrow 24-h range, leading to heterothermy. To investigate the main drivers of heterothermy in a bulk grazer, we compared abdominal temperature, body mass, body condition index, and serum leptin levels in 11 subadult Cape buffalo (Syncerus caffer caffer) during a drought year and a nondrought year. Low food availability during the drought year (as indexed by grass biomass, satellite imagery of vegetation greenness, and fecal chlorophyll) resulted in lower body condition index, lower body mass relative to that expected for an equivalent-aged buffalo, and lower leptin levels. The range of 24-h body temperature rhythm was 2°C during the nondrought year and more than double that during the drought year, and this was caused primarily by a lower minimum 24-h body temperature rhythm during the cool dry winter months. After rain fell and vegetation greenness increased, the minimum 24-h body temperature rhythm increased, and the range of 24-h body temperature rhythm was smaller than 2°C. In order of importance, poor body condition, low minimum 24-h air temperature, and low serum leptin levels were the best predictors of the increase in the range of 24-h body temperature rhythm. While the thermoregulatory role of leptin is not fully understood, the association between range of 24-h body temperature rhythm and serum leptin levels provides clues about the underlying mechanism behind the increased heterothermy in large mammals facing food restriction.

Impacts of Marine Surface Pressure Observations from a Spaceborne Differential Absorption Radar Investigated with an Observing System Simulation Experiment

Abstract A new instrument has been proposed for measuring surface air pressure over the marine surface with a combined active/passive scanning multichannel differential absorption radar to provide an estimate of the total atmospheric column oxygen content. A demonstrator instrument, the Microwave Barometric Radar and Sounder (MBARS), has been funded by the National Aeronautics and Space Administration for airborne test missions. Here, a proof-of-concept study to evaluate the potential impact of spaceborne surface pressure data on numerical weather prediction is performed using the Goddard Modeling and Assimilation Office global observing system simulation experiment (OSSE) framework. This OSSE framework employs the Goddard Earth Observing System model and the hybrid 4D ensemble variational Gridpoint Statistical Interpolation data assimilation system. Multiple flight and scanning configurations of potential spaceborne orbits are examined. Swath width and observation spacing for the surface pressure data are varied to explore a range of sampling strategies. For wider swaths, the addition of surface pressures reduces the root-mean-square surface pressure analysis error by as much as 20% over some ocean regions. The forecast sensitivity observation impact tool estimates impacts on the Pacific Ocean basin boundary layer 24-h forecast temperatures for spaceborne surface pressures that are on par with rawinsondes and aircraft and estimates greater impacts than the current network of ships and buoys. The largest forecast impacts are found in the Southern Hemisphere extratropics.

Air Frosts in Poland in the Thermal Growing Season (AT &gt; 5 °C)

Frosts cause damage to plants in field crops and also trees, thus contributing to heavy economic losses in agriculture. One of the consequences of climate warming is the lengthening of the thermal growing season (AT &gt; 5 °C) and acceleration of phenological phases as well as the lengthening of the frost-free period. This favourable element allows the extension of the range of cultivated plants to include plants requiring warmth and a longer development period. The present study concerns the area of Poland. The data on mean and minimum 24-h period air temperature (200 cm above ground level) were obtained from 52 meteorological stations of the Institute of Meteorology and Water Management—National Research Institute (IMGW-PIB) for the period 1971–2020. A day with air frost was identified when the recorded minimum air temperature was below 0.0 °C and the mean 24-h period air temperature was above 0.0 °C. All calculations concerning frosts were limited to the period with mean 24-h period air temperature &gt;5 °C (the growing season) as determined with the Gumiński method. The obtained results show that in the thermal growing season (AT &gt; 5 °C) in Poland, no statistically significant change in the average number of days with air frosts in the period 1971–2020 was found. On average, in Poland, in the years 1971–2020, a lengthening of the thermal growing season by 6.2 days over 10 years was identified. Earlier disappearance of the latest air frosts in spring was identified as 2 to 3 days over 10 years, and the later occurrence of air frosts in autumn as 1 to 4 days over 10 years. The share of severe (−4.1°C ÷ −6.0 °C) and very severe (&lt;−6.0 °C) frosts in the total number of days with air frosts in Poland amounts to, on average, 5.8% in spring and 2.6% in autumn.

DIFFERENTIAL DETERMINANTS OF AMBULATORY VS. HOME MORNING BLOOD PRESSURE MEASURED BY A SINGLE MULTISENSOR BLOOD PRESSURE MONITORING DEVICE: THE HI-JAMP STUDY BASELINE DATA

Objective: Out-of-office blood pressure (BP) monitoring using ambulatory BP (ABP) monitoring (ABPM) and home BP (HBP) monitoring (HBPM) is recommended for hypertension management. However, BP level measured by ABPM and HBPM may not be consistent even when evaluated the same time-window, morning. This study investigated the determinants of the difference between ABP and HBP in the morning time measured by a multisensor BP monitoring device equipped with a high-sensitivity actigraph and a thermometer. Design and method: Medicated hypertension patients enrolled in the Home-Activity ICT-based Japan Ambulatory Blood Pressure Monitoring Prospective (HI-JAMP) Study consecutively underwent office BP monitoring, 24-h ABPM (with 30-min intervals), and 5-day HBPM (twice each morning and evening) using the same multisensor BP monitoring device (TM-2441; A&amp;D Company, Tokyo). Morning ABP (average of 4 readings within 2-h after waking) and morning HBP (average of HBP readings measured in the morning over a HBPM period) were compared. In addition, this device recorded the wearer's fine-scale physical movement during a 24-h ABPM period and stored temperature data at the time of each BP measurement. Hourly averages of physical activity and temperatures at the time of HBPM (i.e., room temperature) were used for the analysis. Results: Among 2322 patients (males 53.2%, 69.2±11.5 years, average office systolic BP [SBP] 132.8±18.8 mmHg), morning home SBP was 2.4 mmHg higher than morning ambulatory SBP with standard deviation of 15.9 mmHg (ABP &lt; HBP). In a multivariable analysis including conventional risk factors, older age and larger number of antihypertensive agents were significantly associated with [HBP &gt; ABP], while history of cardiovascular disease (CVD) was significantly associated with [ABP &gt; HBP]. In the analysis additionally including physical activity in the morning and room temperature at the morning HBP measurement, higher activity level was significantly contributed to [ABP &gt; HBP] and lower room temperature was significantly contributed to [HBP &gt; ABP]. Conclusions: In treated hypertensive patients, HBPM is recommended especially if the patient is older, taking multiple antihypertensive agents, or living in an environment with lower room temperatures in the winter, while ABPM is recommended if the patient has a history of CVD.

Bacterial development in semen stored at ambient temperature in INRA96®

Sperm motility and mitochondrial activity can be maintained at room temperature for 24h in INRA96®. However bacterial development remains possible. Furthermore, very few studies evaluated the sensitivity of bacteria to the antibiotics included in INRA96. In experiment 1, semen from 8 stallions (28 ejaculates) was diluted to 20 × 106 sperm/mL in INRA96. The total amount of bacteria, dead or alive, was evaluated using Easykit6® (IMV-Technologies) immediately after dilution, then after 24h and 48h. Bacterial concentration remained stable during 48h cooled storage in Neopor® box (Minitube): mean±sem 9.8±2 × 105, 8.5±2 × 105, 8.4±12 × 105 bacteria/mL at 0h, 24h, 48h, respectively. Bacterial concentration significantly increased after 48h at ambient temperature between 13 and 26°C: 9.7±2 × 105a, 10±2 × 105ab, 28±6 × 105b bacteria/mL at 0h, 24h, 48h, and was significantly higher at ambient temperature than in cooled samples both after 24h and 48h. However, when data were analysed individually, the significant increase occured only for 3 of the 8 stallions. In experiment 2, semen from 7 stallions with 3 ejaculates each was evaluated in duplicate after 0h, 24h and 48h at ambient temperature. Easykit6® showed a significant increase in bacterial concentration for 2 stallions. Conventional bacteriology demonstrated an increase in CFU concentration for 3 stallions including the 2 mentioned above, and a decrease for another stallion. For example, stallion S showed 8±8a, 592±298ab, 8567±4493bCFU/mL at 0h, 24h, 48h, respectively, whereas stallion L showed 1259±504b, 92±53a,117±72aCFU/mL. Sixteen bacterialspecies (38 strains) and one yeast were identified. All the strains of Enterococcus fecalis, Listeria grayi, Streptococcus zooepidemicus, and some strains of Aerococcus viridans, Aeromonas salmonicida, Pasteurella multocida, Pseudomonas sepacia, Staphylococcus hominis, Staphylococcus xylosus, were resistant to both antibiotics of INRA96: 0.028g/L penicillinG and 0.077g/L gentamicin. Ten of the 20 strains identified at 0h and resistant to those antibiotics were not observed anymore at 24h. Eighteen of the 20 strains identified at 48h were resistant to those antibiotics. Eight of them have not been detected at 0h. Candida famata and the 17 bacterial strains identified at 0h and sensitive to those antibiotics were not observed anymore at 24h.The antibiotics and amphotericin B present in INRA96 seem enough to prevent microbial development at ambient temperature, provided that micro-organisms are sensitive to those molecules. Competition occurs between strains: those sensitive to the antibiotics of the extender cannot develop during storage, whereas others, even under the level of detection in raw semen, grow during storage because they are resistant. Bacterial concentration was not significantly correlated with sperm quality (motility, morphological abnormalities). Such bacterial level may not affect spermatozoa. Whether it could induce endometritis in inseminated mares needs investigation. Financial support: IFCE scientific council (Spermicrobes).

Analysis of Vertical Temperature Gradients and Their Effects on Hybrid Girder Cable-Stayed Bridges

The real temperature distribution within 24 h of the main beam in a single-tower hybrid beam cable-stayed bridge is analysed according to its actual section and material parameters, as well as other factors of local atmospheric temperature, geographical environment, and solar intensity. The results show that the internal temperature distribution in the steel–concrete composite beam is uneven, and the temperature of the steel is higher than that at the surface of the concrete slab. Then, a finite element model of the whole bridge is established using the thermal–mechanical sequential coupling function in ABAQUS to acquire the structural response under the action of a 24-h temperature field. The results show that the vertical temperature gradients have a great influence on the longitudinal stress in the lower flange of the steel I-beam, with a maximum compressive stress of 11.9 MPa in the daytime and a maximum tensile stress of 13.36 MPa at midnight. The temperature rise leads to a downward deflection of the main span, and the maximum deflection occurs at the 1/4 main span. There was an obvious temperature gradient in the concrete slab, with a difference between the maximum and minimum value of 14 °C. Similarly, the longitudinal compressive stress of the concrete slab increases with increasing temperature in the daytime, but the peak time is obviously inconsistent with that of the steel beam.

Temminck pangolins relax the precision of body temperature regulation when resources are scarce in a semi-arid environment.

Climate change is impacting mammals both directly (for example, through increased heat) and indirectly (for example, through altered food resources). Understanding the physiological and behavioural responses of mammals in already hot and dry environments to fluctuations in the climate and food availability allows for a better understanding of how they will cope with a rapidly changing climate. We measured the body temperature of seven Temminck's pangolins (Smutsia temminckii) in the semi-arid Kalahari for periods of between 4 months and 2 years. Pangolins regulated body temperature within a narrow range (34-36°C) over the 24-hcycle when food (and hence water, obtained from their prey) was abundant. When food resources were scarce, body temperature was regulated less precisely, 24-h minimum body temperatures were lower and the pangolins became more diurnally active, particularly during winter when prey was least available. The shift toward diurnal activity exposed pangolins to higher environmental heat loads, resulting in higher 24-h maximum body temperatures. Biologging of body temperature to detect heterothermy, or estimating food abundance (using pitfall trapping to monitor ant and termite availability), therefore provide tools to assess the welfare of this elusive but threatened mammal. Although the physiological and behavioural responses of pangolins buffered them against food scarcity during our study, whether this flexibility will be sufficient to allow them to cope with further reductions in food availability likely with climate change is unknown.

Effect Of Different Temperature Regimes And Chemicals On Seed Germination Behaviour Of Hippophae Salicifolia D. Don

Hippophae spp. (commonly known as Seabuckthorn) is a hardy deciduous shrub of family Elaeagnaceae, native to the temperate regions of Europe and Asia. Hippophae is considered to include seven species and eight subspecies, one of which is Hippophae salicifolia D. Don is also found in Indian Himalayan region. It is important for medicinal use in traditional to modern system of medicines e.g.- cough, bronchitis and expectorant. The present investigation was carried out to improve seed germination. We applied different chemicals including GA3 (5mM, 10mM, 15mM, 20mM), KNO3 and thiourea (50 mM, 75 mM, 100 mM, 125 mM) at room temperature for 24h. Treated seeds placed in Petri plates containing moistened Whatman filter paper. Petri plates were kept at different temperature regimes (room, 20°C, 25°C and 30°C) in incubator and moistened as per need with distilled water. A set of seeds without pre-sowing treatments were considered as control. The experiment was carried out in Factorial Control Randomized Design with three replications. Various germination parameters were observed and results indicated that among different treatments combinations; T4U4, (temperature 30°C × thiourea @ 100 mM) was found effective in all seed germination parameters but negative effect of this temperature were seen on survival of seedlings therefore treatment combination T3U4, (temperature 25°C × thiourea @ 100 mM) was found more suitable for early germination (8 days), mean daily germination (0.85 percent seeds/day) and germination percent (94.67).

Weather Conditions and Biostimulants Influence Nitrogen Acquisition from Different Sources by Soybean Plants

This study aimed to determine the influence of weather conditions (air temperature, precipitation and insolation) on the quantity of nitrogen taken up by soybean plants whose cultivation included an application of two biostimulants: Asahi and Improver, which have been approved for sale. An isotopic dilution method was used which involved an application of mineral fertilisers enriched with the isotope 15N (5%) to detect the quantity of nitrogen fixed from the atmosphere, acquired from the soil and taken up from the fertiliser. Microplots of 1 m2, organised to form larger units, were planted with soybean. The impact of meteorological conditions on the amount of nitrogen fixed by plants as influenced by the experimental biostimulants was estimated using regression trees based on the C&amp;RT algorithm in STATISTICA 13.3. This procedure yielded regression trees which revealed that, irrespective of the test biostimulant, the quantity of nitrogen fixed from the atmosphere was mainly influenced by the air temperature in July, as indicated by the first and most significant branching of the tree. The poorest fixation of atmospheric nitrogen in plants was observed when the average 24-h air temperature in July was higher than 20.9 °C, the quantities being 20.61, 31.33 and 30.49 kg, respectively, in the control, Asahi- and Improver-treated plots. The superior nitrogen uptake from fertiliser, from 10.64 (for the control) to 14.98 kg (in the Improver-amended units), was found when the air temperatures recorded in July and June did not exceed, respectively, 20.9 and 13.15 °C, and the daily rainfall in July was up to 5.65 mm. The regression tree model associated with the quantity of nitrogen acquired by soybean plants from soil indicates that, just like atmospheric nitrogen and nitrogen taken up from fertiliser, the average daily air temperature in July was the major factor determining the first branching of the tree. When this temperature went beyond 20.9 °C, the lowest uptake of nitrogen from soil was found for control plants.

Association between first 24-h mean body temperature and mortality in patients with diastolic heart failure in intensive care unit: A retrospective cohort study.

Body temperature (BT) has been used to evaluate the outcomes of patients with various diseases. In this study, patients with diastolic heart failure (DHF) in the intensive care unit (ICU) were examined for a correlation between BT and mortality. This was a retrospective cohort study of the Medical Information Mart for Intensive Care (MIMIC)-IV dataset. A total of 4,153 patients with DHF were included. The primary outcomes were 28-day ICU and higher in-hospital mortality rates. BT was used in the analyses both as a continuous variable and as a categorical variable. According to the distribution of BT, the patients were categorized into three groups (hypothermia BT <36.5°C, normal 36.5°C ≤ BT <37.5°C, and hyperthermia BT ≥37.5°C). Multivariate logistic regression analysis was performed to explore the association between BT and patient outcomes. The proportions of the groups were 23.6, 69.2, and 7.2%, respectively. As a continuous variable, every 1°C increase in BT was associated with a 21% decrease in 28-day ICU mortality (OR: 0.79, 95% CI: 0.66-0.96, and p = 0.019) and a 23% decrease in in-hospital mortality (OR: 0.77, 95% CI: 0.66-0.91; and p = 0.002). When BT was used as a categorical variable, hypothermia was significantly associated with both 28-day ICU mortality (OR: 1.3, 95% CI: 1.03-1.65; and p = 0.026) and in-hospital mortality (OR: 1.31, 95% CI: 1.07-1.59; and p = 0.008). No statistical differences were observed between 28-day ICU mortality and in-hospital mortality with hyperthermia after adjustment. The first 24-h mean BT after ICU admission was associated with 28-day ICU and in-hospital mortality in patients with DHF. Hypothermia significantly increased mortality, whereas hyperthermia did not.

Late isocaloric eating increases hunger, decreases energy expenditure, and modifies metabolic pathways in adults with overweight and obesity

Late eating has been linked to obesity risk. It is unclear whether this is caused by changes in hunger and appetite, energy expenditure, or both, and whether molecular pathways in adipose tissues are involved. Therefore, we conducted a randomized, controlled, crossover trial (ClinicalTrials.gov NCT02298790) to determine the effects of late versus early eating while rigorously controlling for nutrient intake, physical activity, sleep, and light exposure. Late eating increased hunger (p&lt;0.0001) and altered appetite-regulating hormones, increasing waketime and 24-h ghrelin:leptin ratio (p&lt;0.0001 and p= 0.006, respectively). Furthermore, late eating decreased waketime energy expenditure (p= 0.002) and 24-h core body temperature (p= 0.019). Adipose tissue gene expression analyses showed that late eating altered pathways involved in lipid metabolism, e.g., p38 MAPK signaling, TGF-β signaling, modulation of receptor tyrosine kinases, and autophagy, in a direction consistent with decreased lipolysis/increased adipogenesis. These findings show converging mechanisms by which late eating may result in positive energy balance and increased obesity risk.