2-formylbenzoic Acid Research Articles

A bioinspired hard-soft composites with strong interfacial bonding, high load-bearing and low friction for osteochondral repair

The development of osteochondral composites that simultaneously exhibit superior load-bearing performance and lubrication properties remains a significant challenge. In this investigation, a novel hard-soft composite material, which utilizes porous Si3N4 ceramics as a structural support and PVA-CBA (synthesis by condensation of polyvinyl alcohol and 4-formylbenzoic acid) hydrogels as a lubrication, was fabricated via impregnating the hydrogel into porous ceramics, namely Si3N4/PVA-CBA composites. It demonstrates strong interfacial bonding, comprising macro-micro scale mechanical interlocking and molecular scale chemical crosslinking. The developed material exhibits high mechanical strength (∼ 103.2 MPa and ∼ 1500 % larger than the corresponding value of porous Si3N4 ceramics), low friction and wear (∼ 0.21 under 10 N load and 10 Hz frequency), and favorable cell adhesion and proliferation. This provides an effective strategy for designing hard-soft composite material in the osteochondral repair field.

Nrf2/FSP1/CoQ10 axis-mediated ferroptosis is involved in sodium aescinate-induced nephrotoxicity

Sodium aescinate (SA), an active compound found in horse chestnut seeds, is widely used in clinical practice. Recently, the incidence of SA-induced adverse events, particularly renal impairment, has increased. Our previous work demonstrated that SA causes severe nephrotoxicity via nephrocyte ferroptosis; however, the underlying mechanism remains to be fully elucidated. In the current study, we investigated additional molecular pathways involved in SA-induced nephrotoxicity. Our results showed that SA inhibited cell viability, disrupted cellular membrane integrity, and enhanced reactive oxygen species (ROS), ferrous iron (Fe2+), and malondialdehyde (MDA) levels, as well as lipid peroxidation in rat proximal renal tubular epithelial cell line (NRK-52E) cells. SA also depleted coenzyme Q10 (CoQ10, ubiquinone) and nicotinamide adenine dinucleotide (NADH) and reduced ferroptosis suppressor protein 1 (FSP1) and polyprenyltransferase (coenzyme Q2, COQ2) activity, triggering lipid peroxidation and ROS accumulation in mouse kidneys and NRK-52E cells. The overexpression of COQ2, FSP1, or CoQ10 (ubiquinone) supplementation effectively attenuated SA-induced ferroptosis, whereas iFSP1 or 4-formylbenzoic acid (4-CBA) pretreatment exacerbated SA-induced nephrotoxicity. Additionally, SA decreased nuclear factor-erythroid-2-related factor 2 (Nrf2) levels and inhibited Nrf2 binding to the −1170/-1180 bp ARE site in FSP1 promoter, resulting in FSP1 suppression. Overexpression of Nrf2 or its agonist dimethyl fumarate (DMF) promoted FSP1 expression, thereby improving cellular antioxidant capacity and alleviating SA-induced ferroptosis. These results suggest that SA-triggers renal injury through oxidative stress and ferroptosis, driven by the suppression of the Nrf2/FSP1/CoQ10 axis.

Co-crystals: The neutral molecules in modification of crystal structure of [W(CN)6(bpy)]2− anion salts

Ten new coformers were incorporated into structure of (PPh4)2[W(CN)6(bpy)] salt (bpy = 2,2′-bipyridine), this includes such neutral molecules as: 1,10-phenanthroline, 4-hydroxyacetophenone, 2-amino-5-nitrobenzonitrile, 5-amino-2-pyridinecarbonitrile, 2-nitrobenzonitrile, 4-formylbenzoic acid, 4-pyridinecarbonitrile, 2-pyridinecarbonitrile, 2-hydroxybenzonitrile and 2-naphtol. The composition of cocrystals were confirmed by elemental analysis while the incorporation of coformers was also proved by IR spectra. The single crystal structures were solved and described for salts with 2-naphtol and 4-hydroxyacetophenone. The formation of crystals was caused by formation of strong hydrogen bonds between coformers and cyanido ligands of the anion. The structures were compared and discussed with other similar systems. Additionally, the influence of the coformer on the packing structure of hexacyanido tungsten(IV) complexes was discussed.

Organotin(IV)-Decorated Graphene Quantum Dots as Dual Platform for Molecular Imaging and Treatment of Triple Negative Breast Cancer.

The pharmacological activity of organotin(IV) complexes in cancer therapy is well recognized but their large applicability is hampered by their poor water solubility. Hence, carbon dots, in particular nitrogen-doped graphene quantum dots (NGQDs), may be a promising alternative for the efficient delivery of organotin(IV) compounds as they have a substantial aqueous solubility, a good chemical stability, and non-toxicity as well as a bright photoluminescence that make them ideal for theranostic applications against cancer. Two different multifunctional nanosystems have been synthesized and fully characterized based on two fragments of organotin-based cytotoxic compounds and 4-formylbenzoic acid (FBA), covalently grafted onto the NGQDs surface. Subsequently, an in vitro determination of the therapeutic and theranostic potential of the achieved multifunctional systems was carried out. The results showed a high cytotoxic potential of the NGQDs-FBA-Sn materials against breast cancer cell line (MDA-MB-231) and a lower effect on a non-cancer cell line (kidney cells, HEK293T). Besides, thanks to their optical properties, the dots enabled their fluorescence molecular imaging in the cytoplasmatic region of the cells pointing towards a successful cellular uptake and a release of the metallodrug inside cancer cells (NGQDs-FBA-Sn).

Investigation of dipodal acetoguanamine-Schiff bases and their homonuclear FeIII complexes

The ligands 2,4-bis(2-hydroxyphenyliminomethyl)-6-methyl-1,3,5-triazine (AGSAL), 2,4-bis(2-carboxyphneyliminomethyl)-6-methyl-1,3,5-triazine (AG2FBA), and 2,4-bis(4-carboxyphneyliminomethyl)-6-methyl-1,3,5-triazine (AG4FBA) were prepared with a Schiff base condensation between acetoguanamine and the corresponding salicylaldehyde, 2-formylbenzoic acid, and 4-formylbenzoic acid. High-spin (S = 5/2) complex [FeIII(AGSAL)Cl] was obtained by complexation of AGSAL with FeCl3. Then, binuclear complexes [{FeIII(AGSAL)}2(AG2FBA)] and [{FeIII(AGSAL)}2(AG4FBA)] were prepared by combination of [FeIII(AGSAL)Cl] and “(AG2FBA), (AG4FBA),” respectively. Then, trinuclear complexes [{FeIII(AGSAL)}2(FeIIIAG2FBA)] and [{FeIII(AGSAL)}2(FeIIIAG4FBA)] of the ligands were prepared by reaction of the binuclear complexes and FeCl3 salt. A weak antiferromagnetic exchange was found for all complexes. Finally, the structures of ligands and complexes were identified by using elemental analysis, magnetic susceptibility, 1H NMR, 13C NMR and FT-IR spectral data.

Synthesis of Novel Porphyrin Derivatives and Investigate their Application in Sensitized Solar Cells

Solar energy has significant advantages compared to conventional sources such as coal and natural gas, including no emissions, no need for fuel, and the potential for installation in a wide range of locations with access to sunlight. In this investigation, heterocyclic derivatives were synthesized from several porphyrin derivatives (4,4',4",4"'-(porphyrin-5,10,15,20-tetrayl) tetra benzoic acid) compound (3), obtained by reaction Pyrrole with 4-formyl benzoic acid. Subsequently, porphyrin derivative-component amides 5a, 5b, and 5c were produced by reacting compound (3) with amine derivatives at a 1:4 molar ratio. These derivatives exhibited varying sensitivities for utilization in solar cells, with compound 5a displaying the highest power conversion efficiency (PCE) at 1.37%, as determined by measuring the short circuit current (Jsc), open-circuit voltage (Voc), and fill factor (FF) (Jsc = 2.24 mA cm-2, Voc = 0.80 mV, FF = 76.5%). Meanwhile, compound 5c exhibited the lowest PCE at 0.94% (Jsc = 1.55 mA cm-2, Voc = 0.750 mV, FF = 76.4%).

A High-Efficient DOPO-Based Flame Retardant as a Co-Curing Agent for Simultaneously Enhancing the Fire Safety and Mechanical Properties of Epoxy Resin.

Simultaneously enhancing the fire safety and mechanical properties of epoxy resin (EP) remains a persistent challenge. Herein, a high-efficient phosphaphenanthrene-based flame retardant (FNP) is synthesized using 3,5-diamino-1,2,4-triazole, 4-formylbenzoic acid, and 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide. Due to the presence of active amine groups, FNP is employed as a co-curing agent for fabricating EP composites with outstanding fire safety and mechanical properties. EP containing 8 wt% FNP (EP/8FNP) achieves a vertical burning (UL-94)V-0 rating with a limiting oxygen index of 31%. Meanwhile, FNP declines the peak heat release rate, total heat release, and total smoke release of EP/8FNP by 41.1%, 31.8%, and 16.0%, respectively, compared to those of unmodified EP. The increased fire safety of EP/FNP composites is because FNP promotes the formation of an intumescent, compact, and cross-linking char layer for EP/FNP composites, and releases P-containing substances and noncombustible gases in the gas phase during combustion. In addition, EP/8FNP exhibits 20.3% and 5.4% increase in the flexural strength and modulus compared with those of pure EP. Furthermore, FNP enhances the glass transition temperature of EP/FNP composites from 141.6 °C for pure EP to 147.3 °C for EP/8FNP. Therefore, this work is conducive to the future development of fabricating fire-safe EP composites with enhanced mechanical properties.

Synthesis of novel dihydropyridine, fused dihydropyridines, hexahydrobenzo[4,5]imidazo[2,1-b]quinazolines, and fused-pyrans linked to piprazine core via benzoyloxyacetyl likages as new hybrid molecules utilizing Michael, and Hantzsch reaction

The precursor, piperazine-1,4-diylbis(2-oxoethane-2,1-diyl) bis(4-formylbenzoate) was prepared by reacting 1,1’-(piperazine-1,4-diyl)bis(2-chloroethan-1-one) with the potassium salt of 4-formylbenzoic acid in DMF at reflux. A new series of 1,4-dihydropyridin-3,5-dicarbonitrile, hexahydroacridine-1,8-diones, decahydropyrimido[4,5-b]quinoline, and hexahydrobenzo[4,5]imidazo[2,1-b]quinazolines which are linked to piperazine-1,4-diylbis(2-oxoethane-2,1-diyl) dibenzoate were obtained through the Hantzsch reaction. Besides, the Michael addition reaction of the piperazine-bis-aldehyde with malononitrile and active methylene containing reagents in the presence of catalytic amounts of a basic catalyst yielded the corresponding fused pyrans. The constitutions of novel compounds were confirmed using the different spectral tools.

Poly(vinyl alcohol-co-vinyl acetal) gel electrolytes for alkaline water electrolysis

A series of poly(vinyl alcohol-co-vinyl acetal) gel electrolytes was synthesized, characterized and assessed as electrode separators in alkaline water electrolysis. The copolymers were prepared by reacting poly(vinyl alcohol) with benzaldehyde or 4-formylbenzoic acid under acidic conditions at different ratios, and visually homogenous and water-insoluble membranes were subsequently obtained by solution casting. The physicochemical characteristics in terms of electrolyte uptake, swelling behavior, and ion conductivity could be tuned by varying the degree of functionalization. At a moderate vinyl acetal content of 5%, the membrane combined mechanical robustness with ion conductivity reaching 36 mS cm−1 in 30 wt% aqueous KOH at room temperature. Current densities of up to 1000 mA cm−2 were reached with uncatalyzed Ni-foam electrodes at a cell voltage of less than 2.6 V in alkaline water electrolysis tests, while the membrane effectively prevented hydrogen crossover. Although apparent membrane degradation was observed after a few days of electrolysis operation, the strategies presented in this work to tune membrane properties are of general relevance to the field towards the development of new ion-solvating membrane systems based on more alkaline stable and robust backbone chemistries.

Cytochrome P450-catalyzed oxidation of halogen-containing substrates

Cytochrome P450 (CYP) enzymes are heme-thiolate monooxygenases which catalyze the oxidation of aliphatic and aromatic C-H bonds and other reactions. The oxidation of halogens by cytochrome P450 enzymes has also been reported. Here we use CYP199A4, from the bacterium Rhodopseudomonas palustris strain HaA2, with a range of para-substituted benzoic acid ligands, which contain halogens, to assess if this enzyme can oxidize these species or if the presence of these electronegative atoms can alter the outcome of P450-catalyzed reactions. Despite binding to the enzyme, there was no detectable oxidation of any of the 4-halobenzoic acids. CYP199A4 was, however, able to efficiently catalyze the oxidation of both 4-chloromethyl- and 4-bromomethyl-benzoic acid to 4-formylbenzoic acid via hydroxylation of the α‑carbon. The 4-chloromethyl substrate bound in the enzyme active site in a similar manner to 4-ethylbenzoic acid. This places the benzylic α‑carbon hydrogens in an unfavorable position for abstraction indicating a degree of substrate mobility must be possible within the active site. CYP199A4 catalyzed oxidations of 4-(2′-haloethyl)benzoic acids yielding α-hydroxylation and desaturation metabolites. The α-hydroxylation product was the major metabolite. The desaturation pathway is significantly disfavored compared to 4-ethylbenzoic acid. This may be due to the electron-withdrawing halogen atom or a different positioning of the substrate within the active site. The latter was demonstrated by the X-ray crystal structures of CYP199A4 with these substrates. Overall, the presence of a halogen atom positioned close to the heme iron can alter the binding orientation and outcomes of enzyme-catalyzed oxidation.

Enzyme-Catalyzed Dynamic Kinetic Resolution of 2-Formylbenzoic Acids for the Asymmetric Synthesis of Phthalidyl Esters and Related Prodrugs.

An enzyme-catalyzed dynamic kinetic resolution strategy was applied for the asymmetric synthesis of phthalidyl esters in high yields (up to 95%) and enantiomeric purities (up to 99% ee) through a direct one-pot procedure. Preparation of phthalidyl ester prodrugs and a scale-up reaction demonstrated the potential of this method for practical applications.

A New Boron-Rhodamine-Containing Carboxylic Acid as a Sugar Chemosensor.

We propose a boron-rhodamine-containing carboxylic acid (BRhoC) substance as a new sugar chemosensor. BRhoC was obtained by the Friedel-Crafts reaction of 4-formylbenzoic acid and N,N-dimethylphenylboronic acid, followed by chloranil oxidation. In an aqueous buffer solution at pH 7.4, BRhoC exhibited an absorption maximum (Absmax) at 621 nm. Its molar absorption coefficient at Absmax was calculated to be 1.4 × 105 M-1 cm-1, and it exhibited an emission maximum (Emmax) at 644 nm for the excitation at 621 nm. The quantum yield of BRhoC in CH3OH was calculated to be 0.16. The borinate group of BRhoC reacted with a diol moiety of sugar to form a cyclic ester, which induced a change in the absorbance and fluorescence spectra. An increase in the D-fructose (Fru) concentration resulted in the red shift of the Absmax (621 nm without sugar and 637 nm with 100 mM Fru) and Emmax (644 nm without sugar and 658 nm with 100 mM Fru) peaks. From the curve fitting of the plots of the fluorescence intensity ratio at 644 nm and 658 nm, the binding constants (K) were determined to be 2.3 × 102 M-1 and 3.1 M-1 for Fru and D-glucose, respectively. The sugar-binding ability and presence of a carboxyl group render BRhoC a suitable building block for the fabrication of highly advanced chemosensors.

The efficacy of 2-formyl benzoic acid in reactivating diazinon inhibited murine cholinesterase.

Oximes, as classical acetylcholinesterase (AChE) reactivators, have some pharmacokinetics/pharmacodynamics disadvantages. During the synthesis of non-oxime compounds, we encountered the compound 2-formylbenzoic acid (2-FBA) with promising in vitro and in vivo cholinesterase (ChE) reactivating properties in the acute exposure to diazinon (DZN). For in vitro experiments, the healthy mice serum and brain homogenate were freshly prepared and exposed to DZN (160 µg/mL). After 10 minutes, 2-FBA was added to the poisoned samples, and ChE activity was measured afterward. For the in vivo assay, the mice were poisoned with DZN subcutaneous (SC) injection (50 mg/kg), and after 1 hour, either 2-FBA or Pralidoxime (2-PAM) was injected intravenously (IV). After 3 h, ChE activity was measured in the serum and brain homogenate samples. The LD50 (IV) for 2-FBA in mice was measured as well. 2-FBA effectively reactivated the inhibited ChE in serum and brain homogenate samples in vitro. In the in vivo experiments, while 2-FBA could significantly reactivate the brain ChE even better than 2-PAM, they failed to reactivate the serum ChE by single IV injection. LD50 of 2-FBA was calculated to be 963 mg/kg. There were no general toxicity signs in any treatment groups. The in silico results support the potential ability of 2-FBA efficacy via possibly Witting reaction mechanism. Our findings indicate that 2-FBA seems to be a suitable non-oxime candidate for AChE reactivation with minimal side effects. Further toxicokinetic studies on this compound are strongly recommended to be performed before conducting the clinical trial in humans.

Diastereoselective multicomponent synthesis of dihydroisoindolo[2,1-a]quinolin-11-ones mediated by eutectic solvents.

In this contribution, a series of dihydroisoindolo[2,1-a]quinolin-11-ones was synthesized by a one-pot multicomponent Povarov reaction starting from anilines, alkenes (trans-anethole, methyl eugenol and indene) and 2-formylbenzoic acid. Different eutectic solvents bearing Lewis or Brønsted acids were evaluated as reaction media and catalysts for the model reaction employing p-toluidine and trans-anethole finding that the eutectic mixture ChCl/ZnCl2 (1/2) allowed the obtention of the target compound in 77% isolated yield. Under the optimized reaction conditions, 20 derivatives were obtained in good to moderated yields using meta- and para-susbstituted anilines, while the corresponding ortho-analogs followed a different pathway affording isoindolinones. In addition, the eutectic mixture was reused in six cycles without observing a detrimental catalytic activity. This methodology features mild reaction conditions, short reaction time, simple work-up, and utilization of a reusable solvent; and provides straightforward and diastereoselective access to these alkaloid-like heterocyclic molecules.

Synthesis of dibenzoylmethane-flavonoid hybrids as potential uv filters. Hybrids of chalcones

Background: It has been reported that chalcones (a family of flavonoids) have activity as UV rays absorbers. Previously we have reported that the compounds2´-hydroxy-4- methoxychalcone and 2´-hydroxy-4-methoxy dibenzoylmethane have the basic properties of UVA filters (both show their maximum absorption in the range 350-370 nm with good photostability) Objective: in this work, we describea new series of chalcone-dibenzoylmethane hybrid candidates as synthetic UV filtersand flavonoids Method: The compounds resultedfrom multi step synthesisstarting from 4-formylbenzoic acid and 2´- hydroxy acetophenone. Purification was done by recrystallization or column chromatography. Results: The compounds were obtained with good yields and characterized by spectroscopy. Conclusion: They are good candidates for UVA filters in cosmetics. Drs. Svarc and Minaberry will performthe corresponding photostability studies.

Well-Defined pH-Responsive Self-Assembled Block Copolymers for the Effective Codelivery of Doxorubicin and Antisense Oligonucleotide to Breast Cancer Cells.

The worldwide steady increase in the number of cancer patients motivates the development of innovative drug delivery systems for combination therapy as an effective clinical modality for cancer treatment. Here, we explored a design concept based on poly(ethylene glycol)-b-poly(2-(dimethylamino)ethyl methacrylate)-b-poly(2-hydroxyethyl methacrylate-formylbenzoic acid) [PEG-b-PDMAEMA-b-P(HEMA-FBA)] for the dual delivery of doxorubicin (DOX) and GTI2040 (an antisense oligonucleotide for ribonucleotide reductase inhibition) to MCF-7 breast cancer cells. PEG-b-PDMAEMA-b-PHEMA, the precursor copolymer, was prepared through chain extensions from a PEG-based macroinitiator via two consecutive atom transfer radical polymerization (ATRP) steps. Then, it was modified at the PHEMA block with 4-formylbenzoic acid (FBA) to install reactive aldehyde moieties. A pH-responsive polymer-drug conjugate (PDC) was obtained by conjugating DOX to the polymer structure via acid-labile imine linkages, and subsequently self-assembled in an aqueous solution to form DOX-loaded self-assembled nanoparticles (DOX-SAN) with a positively charged shell. DOX-SAN condensed readily with negatively charged GTI2040 to form GTI2040/DOX-SAN nanocomplexes. Gel-retardation assay confirmed the affinity between GTI2040 and DOX-SAN. The GTI2040/DOX-SAN nanocomplex at N/P ratio of 30 exhibited a volume-average hydrodynamic size of 136.4 nm and a zeta potential of 21.0 mV. The pH-sensitivity of DOX-SAN was confirmed by the DOX release study based on the significant cumulative DOX release at pH 5.5 relative to pH 7.4. Cellular uptake study demonstrated favorable accumulation of GTI2040/DOX-SAN inside MCF-7 cells compared with free GTI2040/DOX. In vitro cytotoxicity study indicated higher therapeutic efficacy of GTI2040/DOX-SAN relative to DOX-SAN alone because of the downregulation of the R2 protein of ribonucleotide reductase. These outcomes suggest that the self-assembled pH-responsive triblock copolymer is a promising platform for combination therapy, which may be more effective in combating cancer than individual therapies.

Synthesis and biological activities of new hybrid chalcones with benzoic acid ring

A series of E-4-(3-oxo-3-(substituted)prop-1-en-1-yl)benzoic acid derivatives (1-5) were synthesized by the Claisen-Schmidt condensation of various ketones with 4-formylbenzoic acid. The anticholinesterase (AChE and BChE), tyrosinase, and urease inhibition activities of the synthesized compounds (1-5) were examined. It was found that the most active compound against AChE enzyme in anticholinesterase inhibition activity was compound 1. Compound 4 was the most active compound in tyrosinase inhibition activity, while compound 3 was the most active compound in urease psychological activity.

Novel substituted isoindolinones derived from lawsone: synthesis, characterization, theoretical, biological activity and docking studies

An efficient Mannich type multicomponent reaction approach was developed for the synthesis of a series of novel substituted isoindolinones derived from Lawsone, 2-formyl benzoic acid, and various primary amines. This protocol features direct construction of C-N and C-C bonds via metal-free method at room temperature using an environment-friendly solvent. The synthesized isoindolinone compounds 4a-4m were characterized by various spectroscopic techniques such as 1H NMR, 13C NMR, FT-IR, and ESI-MS. Computational studies were carried out by the DFT method using B3LYP/6-31G (d,p) basic set as a tool to analyse the chemical reactivity parameters and comprehend the molecular interaction. The molecular electrostatic potential (MEP), dipole moment, chemical reactivity, and stability features of investigated isoindolinones revealed that 4i, 4j, 4l, and 4m could be promising candidates for further biological activity. The cytotoxic activity of selected compounds was evaluated using MTT assay against HepG2 cell lines (Human liver cancer cell line). The data revealed that screened compounds displayed excellent to moderate activity. The docking examination was executed on a promising liver cancer-associated Alpha fetoprotein (AFP). It showed a positive outcome with minimum binding energy and hydrogen bond interaction. Furthermore, In vivo studies ascertained that synthesized isoindolinone derivatives can lead to enhanced cancer cell death and prevention of tumor growth by restoring serum SGOT and SGPT levels near to normal. It further restores matrix metallopeptidase (MMP-2 and MMP-9 levels) near to control confirms the effectiveness of the compound.

Simple, green and one pot new strategy for synthesis of the phthalimide derivatives

A novel, simple, green, one pot and practical synthetic method is described for the preparation of phthalimide derivatives through a condensation reaction between of 2-formylbenzoic acid and aniline in the presence of Et3N as base and S8 (Octasulfur) as oxidant in solvent free condition. This one-pot protocol provides these valuable desired compounds under mild conditions with excellent efficiency by using simple available starting materials.

Facile modification of hydroxyl group containing macromolecules provides autonomously self-healing polymers through the formation of dynamic Schiff base linkages

Self-healing polymers are the next-generation materials for high-performance structures and play a major role in the field of study and research in materials. Biopolymers with biodegradability and biocompatibility, for understandable reasons, are increasingly becoming the focus of attention. Since numerous natural and synthetic polymers are known to contain free hydroxyl groups in their repeating units, the appropriate modification of these macromolecules through their OH groups can be applied for many different polymers. Here, we used poly(vinyl alcohol) (PVA) as model macromolecule with OH groups and reported the synthetic process and modification possibility of this biocompatible polymer. A series of modified PVA samples were synthesized by functionalization with 4-formylbenzoic acid (4-FBA) and 3,4-diaminobenzoic acid (3,4-DABA) via Steglich esterification reaction, thus, the obtained polymer contains both aldehyde and amino groups which are able to form autonomously self-healing polymer via Schiff base reaction. ATR-FTIR measurements confirmed the successful modification of the initial PVA, while optical microscopy, scanning electron microscopy, and X-ray micro-computed tomography (micro-CT) measurements verified the self-healing ability of the polymer without the need for any external stimulus or healing agent. According to the mechanical and thermoanalytical testing, the synthesized polymer shows not only the characteristics of pristine PVA (thermoplastic, biocompatible, biodegradable) but also has advantageous mechanical properties (elasticity, relatively high extensibility) that can be tuned by varying the degree of functionalization. The aforementioned synthetic procedure offers a simple solution for the functionalization of OH group containing polymers, even with low (1-–3 mol%) hydroxyl group content. In this work, the first candidate of a new class of functional polymers is demonstrated that may have promising potential applications in the field of biomedicine or could be used as structural components such as coatings and medical implants.