The Rungwe Volcanic Province (RVP) in the East African Rift System, SW Tanzania, provides a unique opportunity to investigate geothermal resources in the context of particularly complex continental rifting processes. To support geothermal resource targeting in the RVP, we present a revised neotectonic structural map based on an interpretation of aeromagnetic data constrained by 2D-forward modelling of magnetic anomalies integrated with the distribution of previously reported faults, seismic epicentre locations, 3D magnetotelluric resistivity models and surface geothermal manifestations. Magnetic anomalies in the RVP, including the nationally prominent Mbeya anomaly, are related to the high magnetic susceptibility or remanent magnetism of Precambrian rocks and Cretaceous carbonatite intrusions buried in the rift under a varying thickness of non-magnetic sediments and volcanic rocks. Magnetic lineaments are related to structures controlling the geometry of the Precambrian rocks and concealed dikes and the thickness of the sediments and volcanics. The recent Ngozi and Rungwe trachyte volcanics have relatively low magnetic susceptibility comparable to the low susceptibility of the sediments in the rift basins. The revised neotectonic structural map shows prominent NW, NE and NS-trending magnetic lineaments that correlate with previously reported faults and alignments of seismic epicentres in the study area and with the regional trend of the rift segments. The NE-trending magnetic lineaments are consistent with interpretations of the current stress field in the RVP. The main volcanoes in the RVP, Ngozi, Rungwe and Kiejo (also known as Kyejo and Kieyo), are aligned with the NW-trending linear magnetic feature joining the Lupa and Livingstone rift border faults. This lineament is intersected and frequently displaced by the NE and NS-trending lineaments, suggesting that the NE to NS-striking structures are younger. The Rungwe and Ngozi volcanoes as well as numerous ‘’monogenetic’’ eruption centres and the Mwakaleli caldera, which originated ca. 2 Ma ago (Ebinger et al., 1989) following a large explosive eruption emplacing widespread ignimbrite deposits, are located within a zone of low-intermediate magnitude magnetic features forming a basin-like structure surrounded by magnetic high anomalies of the Precambrian basement structures. We interpret the intersections between the NW-trending intra-rift faults and the NS and NE-trending faults as favourable locations for wells to target high permeability within the geothermal resource conceptual models previously constructed using 3D MT resistivity imaging integrated with supporting geoscientific data. The intersections provide a focus area for follow-up ground mapping of subtle features that may be associated with very recent fault movement.