A completely new study of forward-backward multiplicity fluctuation of produced pions in nuclear emulsion detector has been carried out by the method of strongly intensive quantity ΣFB for 16O-AgBr, 28Si-AgBr and 32S-AgBr interactions at 4.5 AGeV/c. Experimental analysis has been compared using MC-RAND events and UrQMD simulated events. UrQMD model could not reproduce the experimental results. Outcome of this analysis supports the presence of dynamical fluctuations in multiparticle production at 4.5 AGeV/c. The results on strongly intensive fluctuation measures at a lower energy show much larger fluctuations in comparison to the higher energy data studied so far.