13C Isotope Discrimination Research Articles

Trapped between drowning and desiccation: Riverine plants under hydropeaking

Hydropeaking is part of hydropower production. The discontinuous release of turbined water during hydropeaking generates sudden rise and falls of the water levels, as well as extended droughts. These artificial flow fluctuations impose challenging growing conditions for riverine vegetation. In order to identify vulnerable/resistant plant species to hydropeaking and to evaluate the impact of contrasting hydropeaking scenarios (simplified (i.e., sudden deep floods, frequent soil saturation and drought) and real-life, power plant-induced scenarios), we measured germination, survival, and morphological and physiological attributes of a selection of 14 plant species commonly found along riparian areas. Species were subject to different hydropeaking scenarios during three months (vegetative period) in the field and in a greenhouse. Half of the species performed worse under hydropeaking in comparison to the control (e.g., less germination and biomass, lower growth rates, reduced stem and root length, physiological stress) but none of the tested hydropeaking scenarios was clearly more disruptive than others. Betula pubescens, Alnus incana and Filipendula ulmifolia showed the largest vulnerability to hydropeaking, while other species (e.g., Carex acuta) were resistant to it. Both in the field and in the greenhouse, plants in perturbed scenarios accumulated more 13C than in the control scenario indicating limited capacity to perform 13C isotope discrimination and evidencing plant physiological stress. The highest 13C abundances were found under drought or flooding conditions in the greenhouse, and under the highest hydropeaking intensities in the field (e.g., Betula pubescens). Our results suggest that any hydropeaking scheme can be equally detrimental in terms of plant performance. Hydropeaking schemes that combine periods of severe drought with long and frequent flooding episodes may create a hostile environment for riverine species. Further research on “hydropeaking-tolerant” plant traits is key to draw the boundaries beyond which riverine species can germinate, grow and complete their life cycle under hydropeaking.

Carbon and Nitrogen Isotope Composition of the Wood of Pinus sylvestris, Betula pendula and Populus tremula

Proportions of 13C/12C and 15N/14N isotopes were identified in different woody compartments of birch (Betula pendula Roth), pine (Pinus sylvestris L.) and aspen (Populus tremula L.) trunks in preforest-steppe and pine-birch forests of the Middle Urals using mass spectrometry. The data were analyzed and interpreted from the perspective of the biochemical processes of carbon and nitrogen metabolism in leaves, cambial tissue, trunk wood, branches, roots, and soil. A lighter isotopic composition of carbon is characteristic of the leaves and trunk cambium. The trunk wood is characterized by a basal trend for 13C enrichment. The 13C/12C ratio corresponds to the nitrogen content in the trunk wood tissues, indicating metabolic control of carbon fractionation in woody plants. The isotope composition of nitrogen in the trunk cambium was significantly depleted in 15N (δ15N varies from –1.5 to –3.5‰) and δ13C (δ13C varies from –28.3 to –25.8‰). Meanwhile the sapwood (δ15N varies from –2.6 to 1‰ and δ13C varies from –27.5 to –27.1‰) and heartwood (δ15N varies from –2.5 to –3.5‰ and δ13C varies from –26 to –24.5‰) were more enriched in 15N and 13C. The significant difference between the values of 13C and 15N isotope discrimination consequently in the different woody compartments of trunk should be taken into account when interpreting data of wood organic matter in the context of paleoclimate.

Elevated CO2 improves yield and N2 fixation but not grain N concentration of faba bean (Vicia faba L.) subjected to terminal drought

Legumes grown in Mediterranean environments frequently experience terminal drought which reduces yield and N2 fixation processes. Decreased N2 fixation during reproductive phases may constrain seed nitrogen concentrations ([N]), reducing protein concentration of grain legumes. Plants grown under elevated atmospheric CO2 concentrations ([CO2]) have greater water use efficiency. This may result in reduced use of conserved/stored soil water, potentially helping to reduce soil water deficits later during grain filling. The extent that this process applies to drought sensitive grain legumes, which are extensively cultivated in Mediterranean environments is unclear. The objectives of this study were to investigate yield, N2 fixation and seed N response of faba bean (Vicia faba L. cv. ‘Fiesta’) grown in a dryland Mediterranean-type environment under elevated [CO2]. Plants were grown in soil columns under ambient [CO2] (˜400 ppm) or elevated [CO2] (e[CO2], ˜550 ppm) in a Free-Air CO2 Enrichment (FACE) facility in the field. One sub-group was continuously well-watered (80% field capacity, FC), whereas a second sub-group was exposed to a drought treatment (water was withheld until 30% FC was reached, which was then maintained during the reproductive phases). Biomass, gas exchange, 13C isotopic discrimination, N2 fixation by the natural abundance 15N method, nodulation and soil water content were assessed throughout the crop developmental stages.Initially, plants grown under elevated [CO2] depleted soil water more slowly in the drought treatment than those under ambient [CO2], but as plants grown under elevated [CO2] produced more biomass they used soil water more rapidly, especially towards the critical pod-filling phase. Water savings during the first phase of the drought treatment, through flowering up to the start of pod-filling, were associated with increased yield (+25%) and N2 fixation (+15%) under drought. Elevated [CO2]-induced stimulation of nodulation and nodule density helped maintain N2 fixation under drought, even though nodule activity decreased under the combined effect of e[CO2] and drought from pod-filling onwards. This later stage decrease was associated with decreased carbohydrate and increased amino acid concentrations in nodules, indicating a down-regulation of N2 fixation. Associated with the decrease of N2 fixation during pod-filling, seed N concentration was lower under the combination of e[CO2] and drought. We propose a conceptual model to explain the importance of N2 fixation during the grain filling stage to maintain seed N concentration under e[CO2]. These findings suggest that e[CO2]-induced savings in soil water may mitigate negative effects of drought on yield and N2 fixation of faba bean, without fully compensating the effect of prolonged drought on seed N concentration.

Rubisco carboxylation kinetics and inorganic carbon utilization in polar versus cold-temperate seaweeds.

Despite the high productivity and ecological importance of seaweeds in polar coastal regions, little is known about their carbon utilization mechanisms, especially the kinetics of the CO2-fixing enzyme Rubisco. We analyzed Rubisco carboxylation kinetics at 4 °C and 25 °C in 12 diverse polar seaweed species (including cold-temperate populations of the same species) and the relationship with their ability to use bicarbonate, by using 13C isotope discrimination and pH drift experiments. We observed a large variation in Rubisco carboxylation kinetics among the selected species, although no correlation was found between either the Michaelis-Menten constant for CO2 (Kc) or Rubisco content per total soluble protein ([Rubisco]/[TSP]) and the ability to use bicarbonate for non-green seaweeds. This study reports intraspecific Rubisco cold adaptation by means of either higher Rubisco carboxylation turnover rate (kcatc) and carboxylase efficiency (kcatc/Kc) at 4 °C or higher [Rubisco]/[TSP] in some of the analyzed species. Our data point to a widespread ability for photosynthetic bicarbonate usage among polar seaweeds, despite the higher affinity of Rubisco for CO2 and higher dissolved CO2 concentration in cold seawater. Moreover, the reported catalytic variation within form ID Rubisco might avert the canonical trade-off previously observed between Kc and kcatc for plant Rubiscos.

Changes in Organic Matter of an Oxisol with the Addition of Soybean and Corn Residues, Nitrogen and Phosphorus

ABSTRACTPlants with different photosynthetic cycles (C3 and C4) and different plant parts (root or shoot) contribute in different ways to soil organic carbon (SOC). In addition, phosphate and nitrogen fertilization also act differently on the SOC stock. In this study, roots or shoots of corn (C3) and soybean (C4) plants were incorporated into samples of an Oxisol, with or without the addition of nitrogen (N) and phosphorus (P) and had the emission of carbon (C)- carbon dioxide (CO2) measured during 45 days. Subsequently, soil organic matter fractionation, carbon and nitrogen microbial biomass and 13C isotopic discrimination were performed. The greatest increment in SOC was observed by adding corn plant material rather than soybean material. For both crops, the greatest contribution to SOC was achieved by adding roots as compared to shoots. Phosphorus addition produced greater microbial activity, followed by the addition of NP and then the addition of only N.

δ13C and δ15N from 14C-AMS dated cereal grains reveal agricultural practices during 4300–2000 BC at Arslantepe (Turkey)

In semi-arid environments of the Near East water availability and soil fertility are limiting factors for crop growing and land use is locally adjusted to environmental features. In the last decades stable carbon and nitrogen isotope analyses on archaeobotanical cereal remains have been developed in order to reconstruct water and nutrient sources for grain filling. Diachronic studies on isotope records from single archaeological sites may help distinguish palaeoclimatic changes from human choices in agricultural practices, but they are actually missing.We have analysed 13C isotope discrimination (Δ13C) and N isotope composition (δ15N) on barley, emmer and wheat 14C-AMS dated grains from the archaeological site of Arslantepe, Malatya (South-Eastern Turkey). Our intent is to focus on the exceptionally long-term development of agricultural practices at the site from 4300 to 2000BC.Stable isotope values of cereals show temporal trends in water supplies and manure application. Irrigation was provided to barley crops from 4300 to 3100BC during the rise of centralised political organisation at the site. Different locations of barley fields are suggested from 3100 to 2000BC when domestic economies are attested. In addition, the marked increase of barley δ15N values from 3350 to 3000BC reveals manuring and/or cultivation in pasturelands due to the deposition of animal urea and dung. Wheat could have been grown close to the site, where irrigation water from natural springs was available. Emmer and wheat seem to have been cultivated in the same areas or directly in the same fields. During 3000–2500BC intercropping cultivation is inferred by low δ15N values. The evidence of mixture crops confirms the increase of pasturelands during herders' occupations and the concentration of crop fields possibly around the site.

Genetic gains for physiological traits associated with yield in soft red winter wheat in the Eastern United States from 1919 to 2009

Abstract Wheat (Triticum aestivum L.) breeding strategies can benefit from periodic evaluation of genetic gains for physiological and morphological traits, and their contribution to yield progress over time in a particular environment. The objective of this research was to expand the recent work at Virginia Tech on genetic yield improvement in soft red winter (SRW) wheat and determine the magnitude of progress for several physiological traits in 50 SRW wheat cultivars released from 1919 to 2009. Physiological traits evaluated here were extensively reported in the literature to be relevant for future wheat breeding as they directly contributed to yield increase under optimum and suboptimal environmental conditions; these traits include canopy temperature depression (CTD), flag leaf width (W), flag leaf area (LA), flag leaf dry weight (DW), flag leaf specific area (SLA), SPAD (soil plant analysis development) chlorophyll reading, and grain 13C isotope discrimination (Δ). Replicated experiments were performed at Warsaw and Holland, VA, in 2009–2010 and 2010–2011 growing seasons. Results showed that three traits consistently changed in magnitude over time and, at the same time, were significantly (p

Increased temperature, rather than elevated CO2, modulates the carbon assimilation of the Arctic kelps Saccharina latissima and Laminaria solidungula

Ocean acidification and warming are affecting with special intensity the Arctic Ocean. Arctic coastal ecosystems are dominated by kelp forests with a high biomass production, which are expected to be directly affected by the increases in CO2 and temperature. This study presents the different physiological responses of the Arctic kelps Saccharina latissima and Laminaria solidungula from Kongsfjorden (Svalbard) cultured at 4 and 9 °C in combination with current (390 ppm) and increased (1200 ppm) levels of atmospheric CO2. Both species were largely unaffected by increased CO2 conditions. Carbon fixation was not influenced by CO2, indicating that photosynthesis was C-saturated at present levels, and 13C isotopic discrimination values from algal tissue suggested no deactivation of carbon concentrating mechanisms at increased CO2 levels. Inhibition of photosynthesis by carbonic anhydrases (CAs) inhibitors highlighted the important role of external CAs in inorganic carbon acquisition in Arctic kelps. Saccharina latissima showed a significantly higher growth rate at 9 °C than at 4 °C, probably due to the decrease in the dark respiration rate observed. Growth rate of L. solidungula was not affected by temperature or CO2, and increases in photosynthesis at 9 °C could be partially related to a higher dissolved organic carbon release rate. The photochemical performance of both species was not altered by any of the treatments. These results suggest that S. latissima might be more benefited than L. solidungula in a future warmer Arctic, while both populations seem to be resilient to higher CO2 concentrations.

Ecophysiological responses ofBetula pendula,Pinus uncinataandRhododendron ferrugineumin the Catalan Pyrenees to low summer rainfall

Climate change is producing modifications in the intensity and frequency of rainfall in some regions of the planet. According to predictions, annual rainfall distribution in Western Europe will result in an increase in episodes of drought, thereby negatively affecting nutrient availability. Since high mountain systems will be particularly vulnerable, the physiological and nutritional responses to changes in summer rainfall were monitored over the course of two consecutive summers on three species, which are representative of subalpine forests: birch (Betula pendula Roth.), rhododendron (Rhododendron ferrugineum L.) and mountain pine (Pinus uncinata Mill.). Birch was especially susceptible to scarce precipitation showing alterations in leaf morphology and a decline in net photosynthesis (A) due to stomatal closure, which led to photoinhibition and to early leaf senescence as shown by the photosynthetic nitrogen-use efficiency (PNUE), carbon/nitrogen (C/N) ratio, foliar N and 13C isotope discrimination (Δ13C) results. The Δ13C of the soluble fraction is a good estimator of intrinsic water-use efficiency in this species. Rhododendron and mountain pine had sclerophyllous leaves, as indicated by leaf mass per area, Δ13C, PNUE and C/N results. Rhododendron was particularly affected by short periods of scarce rainfall, which negatively affected gas exchange and photochemistry, and reduced the remobilization of leaf N and P. Mountain pine was the most tolerant species since alterations of gas exchange, photochemistry and Δ13C were not observed. Its highest investment of N in RuBisCo and highest potassium, iron and magnesium leaf concentration contributed to the highest A rates observed.

Increased pCO2 and temperature reveal ecotypic differences in growth and photosynthetic performance of temperate and Arctic populations of Saccharina latissima.

The Arctic population of the kelp Saccharina latissima differs from the Helgoland population in its sensitivity to changing temperature and CO 2 levels. The Arctic population does more likely benefit from the upcoming environmental scenario than its Atlantic counterpart. The previous research demonstrated that warming and ocean acidification (OA) affect the biochemical composition of Arctic (Spitsbergen; SP) and cold-temperate (Helgoland; HL) Saccharina latissima differently, suggesting ecotypic differentiation. This study analyses the responses to different partial pressures of CO2 (380, 800, and 1500 µatm pCO2) and temperature levels (SP population: 4, 10°C; HL population: 10, 17°C) on the photophysiology (O2 production, pigment composition, D1-protein content) and carbon assimilation [Rubisco content, carbon concentrating mechanisms (CCMs), growth rate] of both ecotypes. Elevated temperatures stimulated O2 production in both populations, and also led to an increase in pigment content and a deactivation of CCMs, as indicated by 13C isotopic discrimination of algal biomass (ε p) in the HL population, which was not observed in SP thalli. In general, pCO2 effects were less pronounced than temperature effects. High pCO2 deactivated CCMs in both populations and produced a decrease in the Rubisco content of HL thalli, while it was unaltered in SP population. As a result, the growth rate of the Arctic ecotype increased at elevated pCO2 and higher temperatures and it remained unchanged in the HL population. Ecotypic differentiation was revealed by a significantly higher O2 production rate and an increase in Chl a, Rubisco, and D1 protein content in SP thalli, but a lower growth rate, in comparison to the HL population. We conclude that both populations differ in their sensitivity to changing temperatures and OA and that the Arctic population is more likely to benefit from the upcoming environmental scenario than its Atlantic counterpart.

Carbon Isotopic Data Validate the New Model of Carbon Turnover

A new global redox carbon cycle model is suggested. It claims that lithospheric plates’ movement exerts an impact on photosynthesis development. The impact is realized via periodic injections of CO2 coming from zones of plates’ collisions. Carbon dioxide is derived from oxidation of sedimentary organic carbon in thermochemical sulfate reduction proceeding in subduction zones. Carbon turnover is considered as a conversion of the element from the oxidized state (CO2 + HCO3- + CO3-) into the reduced state produced in photosynthesis and in the following transformation. The isotopic data confirm the validity of the model. They explain the observed correlation of carbon isotope composition of sedimentary organic matter with geologic age. It was found that the difference between carbon isotope composition of organic matter and that of coeval carbonates is an analog of the carbon 13C isotope discrimination in photosynthesis used for modern plants. The periodicity of isotopic characteristics correlates with periodicity of climatic changes, mass extinctions, with the irregularity of stratigraphic distribution of rocks rich in organic matter and other periodic events in biosphere.

Edaphic, structural and physiological contrasts across Amazon Basin forest–savanna ecotones suggest a role for potassium as a key modulator of tropical woody vegetation structure and function

Abstract. Sampling along a precipitation gradient in tropical South America extending from ca. 0.8 to 2.0 m a−1, savanna soils had consistently lower exchangeable cation concentrations and higher C / N ratios than nearby forest plots. These soil differences were also reflected in canopy averaged leaf traits with savanna trees typically having higher leaf mass per unit area but lower mass-based nitrogen (Nm) and potassium (Km). Both Nm and Km also increased with declining mean annual precipitation (PA), but most area-based leaf traits such as leaf photosynthetic capacity showed no systematic variation with PA or vegetation type. Despite this invariance, when taken in conjunction with other measures such as mean canopy height, area-based soil exchangeable potassium content, [K]sa , proved to be an excellent predictor of several photosynthetic properties (including 13C isotope discrimination). Moreover, when considered in a multivariate context with PA and soil plant available water storage capacity (θP) as covariates, [K]sa also proved to be an excellent predictor of stand-level canopy area, providing drastically improved fits as compared to models considering just PA and/or θP. Neither calcium, nor magnesium, nor soil pH could substitute for potassium when tested as alternative model predictors (ΔAIC > 10). Nor for any model could simple soil texture metrics such as sand or clay content substitute for either [K]sa or θP. Taken in conjunction with recent work in Africa and the forests of the Amazon Basin, this suggests – in combination with some newly conceptualised interacting effects of PA and θP also presented here – a critical role for potassium as a modulator of tropical vegetation structure and function.

Identifying resource competition in maize-based soil conservation systems using 13C and 15N isotopic discrimination

Soil conservation measures such as establishing grass barriers or cover crops effectively control erosion but also provoke competition, which reduces yields of companion crops. We used 13C and 15N natural abundance profiles to identify the causes of competition of soil conservation measures on a field with 59% slope in Northwest Vietnam three years after establishment. Treatments were maize under farmer’s practice (T1, control), maize with Guinea grass barriers (T2), maize under minimum tillage (MT) with Pinto peanuts as cover crop (T3), and maize under MT and relay cropped with Adzuki beans (T4). A pretest using data from zero-N plots revealed that abundance of water and limited nitrogen availability induced low grain N concentrations, enriched leaf δ13C, and reduced maize grain yield. Similar low N leaf concentrations and elevated δ13C values were observed in maize growing close to frequently pruned grass barriers under positive water balance conditions, indicating that yield decline in these rows can be attributed mainly to N competition. Enriched δ15N values of maize from rows next to barriers indicated reliance on soil N rather than on 15N-depleted fertiliser N. Vigorous cover crop growth under MT resulted in maize yield decline due to N competition while relay-cropped legumes did not trigger inter-species competition having a similar maize yield, leaf N concentration, δ13C, and δ15N as the control.

Increased CO2 modifies the carbon balance and the photosynthetic yield of two common Arctic brown seaweeds: Desmarestia aculeata and Alaria esculenta

Ocean acidification affects with special intensity Arctic ecosystems, being marine photosynthetic organisms a primary target, although the consequences of this process in the carbon fluxes of Arctic algae are still unknown. The alteration of the cellular carbon balance due to physiological acclimation to an increased CO2 concentration (1300 ppm) in the common Arctic brown seaweeds Desmarestia aculeata and Alaria esculenta from Kongsfjorden (Svalbard) was analysed. Growth rate of D. aculeata was negatively affected by CO2 enrichment, while A. esculenta was positively affected, as a result of a different reorganization of the cellular carbon budget in both species. Desmarestia aculeata showed increased respiration, enhanced accumulation of storage biomolecules and elevated release of dissolved organic carbon, whereas A. esculenta showed decreased respiration and lower accumulation of storage biomolecules. Gross photosynthesis (measured both as O2 evolution and 14C fixation) was not affected in any of them, suggesting that photosynthesis was already saturated at normal CO2 conditions and did not participate in the acclimation response. However, electron transport rate changed in both species in opposite directions, indicating different energy requirements between treatments and species specificity. High CO2 levels also affected the N-metabolism, and 13C isotopic discrimination values from algal tissue pointed to a deactivation of carbon concentrating mechanisms. Since increased CO2 has the potential to modify physiological mechanisms in different ways in the species studied, it is expected that this may lead to changes in the Arctic seaweed community, which may propagate to the rest of the food web.

Combining δ13C measurements and ERT imaging: improving our understanding of competition at the crop-soil-hedge interface

Hedgerow cropping decreases erosion in hillside agriculture but also competes for water and nutrients with crops. This study combined two methods for an improved understanding of water and nutrient competition at the crop-soil-hedge interface. δ13C isotopic discrimination in plants and soil electrical resistivity tomography (ERT) imaging were used in a field trial with maize monocropping (MM) vs. leucaena hedgerow intercropping with and without fertilizer (MHF+ and MHF−) in Thailand. Hedges significantly reduced maize grain yield and aboveground biomass in rows close to hedgerows. ERT revealed water depletion was stronger in MM than in MHF+ and MHF- confirming time domain reflectometry and leaf area data. In MHF+, water depletion was higher in maize rows close to the hedge compared to rows distant to hedges and maize grain δ13C was significantly less negative in rows close to hedges (-10.33‰) compared to distant ones (-10.64‰). Lack of N increased grain δ13C in MHF- (-9.32‰, p ≤ 0.001). Both methods were correlated with each other (r = 0.66, p ≤ 0.001). Combining ERT with grain δ13C and %N allowed identifying that maize growth close to hedges was limited by N and not by water supply. Combining ERT imaging and 13C isotopic discrimination approaches improved the understanding of spatial-temporal patterns of competition at the hedge-soil-crop interface and allowed distinguishing between water and N competition in maize based hedgerow systems.

Estatus hídrico y discriminación isotópica de 13C de dos pasturas convencionales de Uruguay

En pasturas mezcla las gramíneas y leguminosas compiten por agua, pero las habilidades competitivas no dependen sólo de sus diferentes capacidades de absorción, sino también de sus capacidades para la regulación de las pérdidas de agua por disminución de la conductancia estomática. Esto se refleja en una menor discriminación isotópica de C (Δ). En este trabajo se estudiaron los cambios en el contenido hídrico (CH) y de Δ de plantas de trébol blanco en una pastura pura y de trébol blanco, lotus y festuca en una pastura mezcla durante un ciclo invierno-primaveral en un experimento localizado en el sur de Uruguay. De invierno a primavera ocurrió un descenso de CH para festuca y trébol blanco en ambas praderas, pero el declive de Δ ocurrió en todas las especies. En primavera existió una relación lineal entre Δ y CH para trébol blanco y festuca, demostrando que Δ fue un buen indicador del estatus hídrico. También se encontraron relaciones entre Δ y varias variables climáticas, dado que las mismas se relacionaron con cambios en la disponibilidad o en la demanda hídrica. Debido a la competencia que ejerció la gramínea por agua, el valor de Δ de trébol blanco fue menor en la pastura mezcla y el rendimiento también disminuyó. Por tanto, el uso de Δ puede resultar útil para evaluar el impacto del déficit hídrico en la productividad y en los cambios de composición florística de praderas mezcla, ya que este indicador es capaz de integrar el estatus hídrico de las especies vegetales.

Relationship between Water Use Efficiency and Î"13C Isotope Discrimination of Safflower (Carthamus tinctorius L.) under Drought Stress

Drought stress is one of the most limiting factors in agricultural productivity because of its highly negative effect on photosynthesis and growth of plants. The main objectives of this study were to determine the performance of four selected safflower genotypes (Remzibey, Dincer, Balci and TRE-ASL09/14) against drought stress. The relationship between water use efficiency (WUE) and δ13C (isotope discrimination) was investigated under well watered (60%) and drought stress (30%) irrigation in controlled conditions in a green house. The result showed that drought stress clearly reduced plant biomass, leaf area, leaf number, relative water content (RWC), specific leaf weight (SLW), WUE and δ13C in all genotypes, while chlorophyll increased under drought stress. There were significant differences between performances of all safflower genotypes in terms of response to drought stress. High WUE and low δ13C discrimination under drought stress were inversely associated with a strong regression relationship (R2=0.75). The analysis of δ13C revealed a substantial variation in water use efficiency among the genotypes under drought stress. It was revealed that low δ13C discrimination types had high WUE, RWC and total biomass under drought stress. Thus, the ability of the low δC genotypes (high water use efficiency, WUE) to maintain higher RWC may provide a good indication of the differences in drought tolerance of safflower genotypes differing in δ13C. Overall, on the basis of the consistent percentage reductions in plant heights, total dry weight, leaf area, RWC, WUE and low δ13C, the TRE-ASL09/14 new breeding line was found to be more drought tolerant when compared with the other safflower hybrids under drought stress. As a result of our study it is suggested that there is a strong relationship between WUE and lower δ13C under drought stress, indicating that it may be used as a selection criterion for developing safflower genotypes with drought tolerance

Landscape Features Impact on Soil Available Water, Corn Biomass, and Gene Expression during the Late Vegetative Stage

Crop yields at summit positions of rolling landscapes often are lower than backslope yields. The differences in plant response may be the result of many different factors. We examined corn (Zea mays L.) plant productivity, gene expression, soil water, and nutrient availability in two landscape positions located in historically high (backslope) and moderate (summit and shoulder) yielding zones to gain insight into plant response differences. Growth characteristics, gene expression, and soil parameters (water and N and P content) were determined at the V12 growth stage of corn. At tassel, plant biomass, N content, 13C isotope discrimination (Δ), and soil water was measured. Soil water was 35% lower in the summit and shoulder compared with the lower backslope plots. Plants at the summit had 16% less leaf area, biomass, and N and P uptake at V12 and 30% less biomass at tassel compared with plants from the lower backslope. Transcriptome analysis at V12 indicated that summit and shoulder‐grown plants had 496 downregulated and 341 upregulated genes compared with backslope‐grown plants. Gene set and subnetwork enrichment analyses indicated alterations in growth and circadian response and lowered nutrient uptake, wound recovery, pest resistance, and photosynthetic capacity in summit and shoulder‐grown plants. Reducing plant populations, to lessen demands on available soil water, and applying pesticides, to limit biotic stress, may ameliorate negative water stress responses.

Winter Wheat Crop Reflectance and Nitrogen Sufficiency Index Values are Influenced by Nitrogen and Water Stress

In‐season N applications to winter wheat (Triticum aestivum L.) may increase profits and improve N fertilizer accuracy. The objectives of this experiment were to determine the impact of N and water stress on crop reflectance and N sufficiency index (SI) values. The experiment contained five N rates, two water treatments, and four blocks. Crop reflectance was measured at the stem extension and flag leaf growth stages, sufficiency index (SI)‐NDVIwf was ratio between the underfertilized normalized difference vegetation index value {NDVI = [near infrared (NIR)‐red]/[NIR+red]} and the NDVI value from well fertilized and well watered treatments, while SI‐NDVImz was ratio between underfertilized NDVI values and NDVI values from well fertilized plots within a water stress treatment. Yield losses due to water and N stress were determined using 13C isotopic discrimination. This research shows that: (i) at the stem extension and flag leaf growth stages, water stress and N stress increased, green, red, and red‐edge reflectance and reduced NDVI values (ii) following the economic optimum nitrogen rate (EONR) produced grain with greater than 120 g kg−1 protein and <10 min stability; (iii) at stem elongation and flag leaf, N fertilizer induced yield gains had a stronger relationship with SI‐NDVImz (stem extension, r = 0.49*; flag leaf, r = 0.51**) than SI‐NDVIwf (stem extension, r = 0.29; flag leaf, r = 0.33); and (iv) SI‐NDVImz had greater fertilizer recommendation accuracy than SI‐NDVIwf. These findings suggest that in wheat production, SI should be referenced to well fertilized areas within a management zone.

Water use efficiency and associated physiological traits of nine naturalized white clover populations in Chile

With 1 figure and 4 tables AbstractWater use efficiency (WUE) of nine naturalized white clover (Trifolium repens L.) populations was evaluated to select parental genotypes for future breeding programmes and to identify associated physiological traits that could be used as selection criteria. A greenhouse experiment was carried out during the spring of 2007 in Chillán, Chile (36º03′S, 72º07′W). The nine naturalized populations plus two controls were planted in pots containing soil as substrate. The plants were grown under two soil water treatments: with water stress (−1 MPa) and without water stress (−0.01 MPa). The experiment was arranged in a randomized complete block design with three replicates. Transpirated water (T) was recorded daily by weighing the pots, shoot DM (leaves + petioles + stolons) production was measured and WUE was calculated (shoot DM/T). Stomatal conductance (gs), chlorophyll content, relative water content, leaf 13C isotopic discrimination, canopy temperature and stolon water potential were also evaluated. The WUE varied significantly (P = 0.05) among populations and increased under drought conditions because of gs reduction. A high WUE was associated with populations that had a low leaf area DM investment and high stolon DM investment.