We experimentally demonstrated all-digital burst clock and data recovery (BCDR) for symmetrical single-wavelength 50 Gb/s four-level amplitude modulation (PAM-4) passive optical network (PON) over the same fiber link at O-band, with the using of 10G-class optics and simple digital signal processing (DSP). The uplink burst frame and BCDR algorithm is used for accurately evaluating PON uplink transmission performance. The results show that over 26 dB link loss budget for continuous-mode uplink transmission and over 25 dB for burst-mode uplink transmission at a bit error rate of 3.8 × 10−3 can be achieved, respectively. For symmetrical 50 Gb/s/λ PON uplink burst-mode, there are 1 dB power budget loss less than uplink continuous-mode.