OBJECTIVE To identify milk component alterations that might be useful for detecting cows with rumen indigestion. DESIGN Prospective case-control study. ANIMALS 23 Holstein cows with rumen indigestion (cases) and 33 healthy cohorts (controls) from 1 herd. PROCEDURES Cases were defined as cows between 30 and 300 days postpartum with a > 10% decrease in milk yield for 2 consecutive milkings or > 20% decrease in milk yield from the 10-day rolling mean during any milking, abnormally decreased rumen motility, and no other abnormalities. Each case was matched with 2 healthy cows (controls) on the basis of pen, parity, days postpartum, and mean milk yield. Some cows were controls for multiple cases. All cows underwent a physical examination and collection of a rumen fluid sample for pH measurement at study enrollment. Individual-cow milk yield and milk component data were obtained for the 16 milkings before and after study enrollment. Rumen motility and pH and milk components were compared between cases and controls. RESULTS Rumen motility for cases was decreased from that of controls. Cases had an abrupt increase in milk fat percentage and the milk fat-to-lactose ratio during the 2 milkings immediately before diagnosis of rumen indigestion. Receiver operating characteristic analyses revealed that a 10% increase in the milk fat-to-lactose ratio had the highest combined sensitivity (57%) and specificity (85%) for identifying cows with rumen indigestion. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that a positive deviation in the milk fat-to-lactose ratio might be useful for identifying cows with rumen indigestion.

Objective To determine associations between subclinical Mycobacterium paratuberculosis infection and milk production, milk components, and somatic cell counts of dairy cattle. Design Cross-sectional epidemiologic survey. Animals 23 dairy herds in Wisconsin containing 1,653 adult cows were studied. The herds had above average milk production and a history of bovine paratuberculosis in the herd within the previous 12 months. Procedure All adult cows in the herds were tested for paratuberculosis by use of an absorbed ELISA. Milk yield, fat, protein, and somatic cell count data were retrieved electronically from Dairy Herd Improvement Association records. Results 147 ELISA-positive and 1,506 ELISA-negative cows were identified. ELISA-positive cows had a mature-equivalent milk production of 376 kg (829 lb)/lactation less than that for ELISA-negative herdmates. Significant difference was not found in lactation average percentages of fat and protein, or somatic cell count linear score. When comparing ELISA-positive and -negative cow's current mature equivalent milk with all previous lactations, significant difference was found only from the immediate-preceding lactation. When this difference was examined by parity group, significant difference was confined to cows in the second lactation. Clinical Implications Subclinical paratuberculosis infections, as determined by ELISA, are associated with a 4% reduction in milk yield and add to the already substantial costs of clinical M paratuberculosis infection in the dairy industry. (J Am Vet Med Assoc 1996;208:1872-1876)

To provide an updated evaluation of the efficacy and safety of sometribove zinc suspension (rbST-Zn), a form of recombinant bovine somatotropin, in lactating dairy cows. Meta-analysis. 26 studies published in peer-reviewed journals or reviewed by a regulatory agency. To be included, a study had to involve the use of the rbST-Zn formulation available to US producers in accordance with the label instructions for treatment initiation (57 to 70 days postpartum), dose (500 mg, q 14 d), and route (SC). For cows treated with rbST-Zn, mean milk, 3.5% fat-corrected milk, fat, and protein yields were increased by 4.00, 4.04, 0.144, and 0.137 kg/d (8.8, 8.89, 0.32, and 0.30 lb/d), respectively; however, the concentration of milk components did not change. Pregnancy proportion for the first 2 breeding cycles was increased by 5.4%, and pregnancy proportion for the duration of the trial was reduced by 5.5% for rbST-Zn-treated cows, compared with proportions for untreated cows. Mean body condition score (1 to 5 scale) was reduced by 0.06 points during the period of rbST-Zn use for treated cows. Administration of rbST-Zn had no effect on milk somatic cell count, the number of days to pregnancy, or inseminations per pregnancy; rates of fetal loss, twins, cystic ovaries, clinical lameness, lameness lesions, or traumatic lesions of the integumentary system; and odds of clinical mastitis or culling. Results indicated that rbST-Zn administration to dairy cows effectively increases milk production with no adverse effects on cow health and well-being.

To determine growth, morbidity, and mortality rates in dairy calves fed pasteurized nonsaleable milk versus commercial milk replacer and compare economics of feeding pasteurized nonsaleable milk versus commercial milk replacer in dairy calves. Clinical trial. 438 dairy calves. Calves were assigned at 1 to 2 days of age to be fed pasteurized nonsaleable milk or a commercial milk replacer until weaned. Body weight was measured at the time of study enrollment and at the time of weaning, and any medical treatments administered and deaths that occurred prior to weaning were recorded. A partial budget model was developed to examine the economics of feeding pasteurized nonsaleable milk versus commercial milk replacer. Calves fed conventional milk replacer had significantly lower rates of gain (-0.12 kg/d [-0.26 lb/d]), lower weaning weights (-5.6 kg [-12.3 lb]), higher risk for treatment during the summer and winter months (odds ratio [OR], 3.99), and higher risk of death during the winter months (OR, 29.81) than did calves fed pasteurized nonsaleable milk. The estimated savings of feeding pasteurized nonsaleable milk, compared with milk replacer, was dollars 0.69/calf per day. The estimated number of calves needed to economically justify the nonsaleable milk pasteurization system was 23 calves/d. Results suggest that dairy calves fed pasteurized nonsaleable milk have a higher growth rate and lower morbidity and mortality rates than do calves fed conventional milk replacer. Feeding pasteurized nonsaleable milk could be an economically viable strategy for dairy calf producers.

To determine the effect of paratuberculosis on culling, milk production, and milk quality in infected dairy herds. Cross-sectional study. 689 lactating dairy cows in 9 herds. Milk, blood, and fecal samples were obtained from all cows. Fecal samples were evaluated via mycobacterial culture. Serum samples were tested with a commercially available ELISA for antibodies against Mycobacterium avium subsp paratuberculosis, and preserved milk samples were tested with an ELISA for antibodies against M paratuberculosis. Mixed effect and proportional hazards models were used to determine the effect of paratuberculosis on 305-day milk, fat, and protein production; somatic cell count linear score; and the risk of culling. Cows with positive results of bacteriologic culture of feces and milk ELISA produced less milk, fat, and protein, compared with herdmates with negative results. No difference in 305-day milk or fat production was detected in cows with positive results of serum ELISA, compared with seronegative cows. The 3 survival analyses revealed that cows with positive results of each test were at higher risk of being culled than cows with negative results. Paratuberculosis status, as determined by use of all 3 diagnostic tests, was not associated with milk somatic cell count linear score. Results suggest that for the 9 herds in this study, paratuberculosis significantly decreased milk production and cow longevity.

To compare the nutrient composition of commercially available dog milk replacers with that of dog milk. Prospective, cross-sectional study. 5 dog milk samples and 15 samples of commercial dog milk replacers. Dog milk and milk replacers were analyzed for concentrations of total protein, essential amino acids, sugars, total fat, essential fatty acids, calcium, and phosphorus. Energy density was calculated. Results from milk replacers were compared with the range of the concentration of each nutrient in milk samples from mature dogs as well as the National Research Council (NRC) recommendations for puppy growth. Milk replacers varied widely in caloric density and concentration of nutrients such as calcium, protein, and fat. Calcium concentration was lower in 14 of 15 milk replacers than in the dog milk samples. Docosahexaenoic acid was undetectable in 12 of 15 milk replacers but present in all dog milk samples. All milk replacers had numerous essential nutrients outside of the range of the dog milk samples, and many had concentrations of amino acids, essential fatty acids, calcium, and phosphorus less than the NRC minimal requirement or recommended allowance. Compared with NRC recommendations, some dog milk samples had concentrations of total protein, linoleic acid, calcium, or phosphorus less than the recommended allowance. Results suggested that there was substantial variation in nutrient composition of 15 dog milk replacers and that some products were closer approximations of dog milk than others. Nearly all products would benefit from more appropriate calcium, amino acids, and essential fatty acids concentrations and better feeding directions.

Objective— To evaluate differences in milk production between cows with positive and negative caudal fold tuberculin test results in a Mycobacterium bovis-infected dairy herd. Design— Cross-sectional epidemiologic survey. Animals— 369 Holstein cows with lactation duration between 200 and 360 days. Procedure— The caudal fold tuberculin test was performed. Information on milk production data, parity, calving season, days of lactation, previous milk production, and whether cows had clinical mastitis was obtained from farm records. Composite milk samples were collected and submitted for bacterial culture. Results— 170 cows had positive tuberculin test results, and 199 had negative results. Cows with positive test results produced less milk (mean, 347 kg [763 lb]) than did cows with negative test results after adjusting for variables biologically related to milk production. Calving season (spring, summer) was significantly associated with reduced milk production. Cows with clinical mastitis produced less milk than did cows without clinical mastitis, but the difference was not statistically significant in the multiple regression analysis. Clinical Implications— In this herd, tuberculosis was associated with a 4% decrease in milk production. Milk production losses per cow attributable to calving season (spring, summer) were 3 times those attributable to tuberculosis. However, because of the high prevalence of tuberculosis in this herd, the impact of tuberculosis on total herd milk production was an additional cause for concern. (J Am Vet Med Assoc 1998;213:851-854)

To determine whether Neospora caninum serostatus was associated with milk production among Holstein cattle in Ontario. Case-control study and cross-sectional observational study. 3,702 Holstein cows in 83 herds (case-control study) and 3,162 Holstein cows in 57 herds. Herds in the case-control study were grouped on the basis of N. caninum abortion status. Herds in the observational study were considered representative of Ontario dairy herds. The N. caninum serostatus of individual cows was determined with a kinetic ELISA. Milk production was modeled to compare seropositive with seronegative animals while controlling for parity, days since parturition, and herd clustering. In the case-control study, 305-day milk production of seropositive cows was significantly less than milk production of seronegative cows in herds with abortions attributable to N. caninum infection and in herds with abortions attributable to pathogens other than N. caninum, but not in herds without abortion problems. In the observational study, 305-day milk production for seropositive cows was not significantly different from milk production of seronegative cows. Results suggest that the association between N. caninum serostatus and milk production in Ontario Holstein dairy cattle may depend on abortion status of the herd. In herds with abortion problems, regardless of cause, N. caninum-seropositive cattle produced less milk, whereas in herds without abortion problems, N. caninum-seropositive cattle produced the same amount of milk as seronegative cattle.

To determine whether bulk-tank standard plate counts or plate loop counts and bulk-tank somatic cell counts (SCC) were associated with detection of violative antimicrobial residues in milk from dairy cattle. Longitudinal study. Information for 1994 through 1997 was obtained from a large milk marketing cooperative that operated in multiple states throughout the northeastern and midwestern United States (16,831 herd-years of information from 6,546 farms) and from the Ohio Department of Agriculture Grade-A Milk Certification Program (12,042 herd-years of information from 4,022 farms). Data were analyzed by use of multivariate logistic regression. For both data sets, odds that a violative antibiotic residue would be detected increased as mean SCC for the herd-year increased. Standard plate counts and plate loop counts were not associated with odds that a violative antibiotic residue would be detected. Results of this study suggested that the odds that a violative antibiotic residue would be found in bulk-tank milk increased as mean SCC for the herd-year increased. This suggests that management practices that would be expected to influence SCC may also influence the risk of antibiotic residue violations.

To examine the relationship between lameness and milk yield in dairy cows. Cohort study. 531 dairy cows. Cows affected with lameness were classified into 1 of 3 groups on the basis of type of diseases or lesions observed, including interdigital phlegmon (foot rot), papillomatous digital dermatitis (foot warts), or claw lesions. Cows not affected with lameness were classified as healthy. From Dairy Herd Improvement Association records, 305-day mature equivalent milk yield data were collected at the end of lactation or when the cow left the herd. Milk yield was compared between cows affected with lameness and healthy cows. 167 (31%) cows were affected with lameness during lactation. Lame cows had claw lesions (60%), papillomatous digital dermatitis (31%), or interdigital phlegmon (9%). Milk yield in lame cows with interdigital phlegmon (mean, 17,122 lb) was significantly less, compared with healthy cows (19,007 lb). In this herd, interdigital phlegmon was associated with a 10% decrease in milk production. Lame cows with claw lesions or papillomatous digital dermatitis produced less milk than healthy cows, but the difference was not significant.