The purpose of this work is to analyze the chronology and state of development of chromatographic methods at the Zaporizhzhia State Medical University (ZSMU). A historical overview of the use of chromatographic methods at ZSMU is provided. First of all, emphasis is placed on the introduction of high-performance liquid chromatography and gas-liquid chromatography. The main scientific laboratories and groups that worked and are working at the Zaporizhzhia State Medical University in the field of instrumental chromatography are shown. There were groups at the Department of Biological Chemistry, the Department of Faculty Pediatrics, the Department of Physical and Colloidal Chemistry. Currently, the Department of Physical Colloid Chemistry is actively working on a new laboratory of liquid chromato-mass spectrometry of the Department of Experimental Pharmaceutical Research of the training medical-laboratory center. The laboratory performs research for many departments of ZSMU, including the Faculty of Pharmacy, the Faculty of Postgraduate Education, the Faculty of Medicine. The laboratory develops, validates and uses methods to establish the purity and confirm the molecular weight and structure of new biologically active substances synthesized to search for new drugs, research of impurities in drug substances, determination of biologically active substances in extracts of medicinal plant raw materials, determination of residual amounts drugs in poultry meat and eggs, and other livestock and poultry products, determination of concentrations of disease markers, determination of distribution, metabolism and excretion of drugs.

The effect of carbonization temperature on canarium schweinfhurthii seed shell was investigated. Atili seed shell particles was carbonized at different temperatures of 600, 800, 1000 and 1150°C under inert condition for 60 mins each to obtain char products. The effect of temperature on the properties of char was investigated in detail, using several characterization techniques including, mass yield, elemental analysis, and electrical property measurements while structural transformations were monitored using X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) techniques. The char yield decreased from 30.50 % to 26.55 % as the temperature increases from 600°C to 1150°C. The fixed carbon (82.3 to 92.66 %) and ash content (1.35 to 1.72 %) increase as temperature increase while the volatile matter (12.61 to 4.18 %) and moisture content (3.81 to 1.44 %) decreases. Ultimate analysis showed elemental carbon to increase from 85.96 % to 95.79 %. The electrical conductivity of obtained char improved significantly (1.99 x 10-9 to 7.24 x 10-2 S/cm) as well as the structural, morphology and near graphitization of char. Statistical analysis of the FTIR and XRD data via the principal component analysis showed similarity trend on the effect of temperature on the carbonization products. The improved electrical property, pore development in char morphology as well as the development of near graphitization features suggest possible use as electrode materials.

The review of the development of micellar high-performance liquid chromatography and micellar thin-layer chromatography is presented. The results of the research of the theoretical foundation and the organized solution usage as mobile phases for the analysis of plant and animal biological active substances, medicinal substances and drugs are demonstrated. The investigations had been carried out on the Chemical Metrology Department V.N. Karazin Kharkiv National University and in the State Enterprise “Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines”.

Currently, chromatographic methods are widely used to control the quality of products in the pharmaceutical and food industries. This article is devoted to the systematization of the results of scientific research of the authors on the development, validation and application of a number of chromatographic methods in the creation of generic drugs, as well as in the analysis of medicinal plant materials. The developed methods are validated according to the following parameters: specificity, linearity, accuracy and limit of detection. The specificity of the methods is based on the ability to evaluate the analyte in the presence of other components and is confirmed by using external standards. The accuracy was assessed by the results of the analysis of different weights or by the "added-found" method. For the developed methods, parameters of the suitability of chromatographic systems were established (peak symmetry factor, number of theoretical plates, relative standard deviation of retention time and peak area).

An information about teaching and scientific activities in the field of chromatographic methods of analysis at the Department of Analytical Chemistry of the National Taras Shevchenko University of Kiev for the period from the 70s of the twentieth century till present days given. The perspective directions of the development of academic disciplines and scientific research of the department in this area are outlined.

The ways of development of ion chromatography in Ukraine from the eighties of the last century to the present time are shown. The achievements of research institutes, in particular, the Dumansky Institute of Colloid Chemistry and Water Chemistry of the National Academy of Sciences of Ukraine, in the development of ion chromatographic methods for the analysis of organic and inorganic anions in various objects, including natural and drinking waters, are highlighted. Scientists of Ukraine (A.T. Pilipenko, O.V. Zuy, A.V. Terletskaya) compared commercially available sorbents for ion chromatography – HIKS-1, ANIEKS-N and KANK ASt for their use in water analysis. The studies were carried out on an ion chromatograph "Tsvet-3006" with a conductometric detector. It has been shown that ANIEKS-N with a borate eluent is the best sorbent for the determination of organic monocarboxylic acid ions, and KANK ASt with a carbonate-hydrocarbonate eluent – for the determination of inorganic anions. Ion chromatography has been used to solve a number of practical problems: for the separation of oxoanions  iodate, chlorate and bromate in waters, to control the results in the development of chemiluminescent methods for the determination of chloride, nitrite and nitrate, to establish the mechanism of destruction of anionic surfactants under the action of vacuum ultraviolet radiation and corona discharge. The method was applied to the analysis of iodine-containing bottled waters for bromide ions due to the absence of the interference from iodides. The accumulated experience has contributed to the adoption in Ukraine of four state standards for water quality, based on the use of the ion chromatographic method. It is shown that the method of ion chromatography at the present stage is widely used in various sectors of the national economy: to determine the traces of anions in steam condensate of power plants, to control the anionic composition of water at nuclear power plants, to correct water treatment processes at waterworks, in particular, when analyzing chlorite ions in tap water obtained with the use of chlorine dioxide as a disinfectant.

Scientific research in the field of chromatography has been intensively developed and continues to develop in higher educational institutions of Lviv, research institutes, which is due to the widespread use of chromatographic methods of analysis in the scientific field, at enterprises of Lviv region, in the practice of controlling laboratories. Among the scientific achievements of scientists of Lviv region should be noted research in the field of the use of zeolites and other sorbents in chromatogaphy, the study of chemical reactions, the development and application of methods for analysis in the field of medicine, biology, veterinary, medicine, industrial products and environmental objects. Higher educational institutions of Lviv provide training of qualified chromatography specialists. Established cooperation of scientific laboratories and laboratories in industry, which contributes to the development and implementation of new methods of chromatographic analysis in practice.

Currently, high-performance thin-layer chromatography (HPTLC) is widely used to control product quality in the pharmaceutical and food industries. In the manufacture of food products, the most important requirement is the control of the content of various additives (preservatives, dyes, antioxidants). For the first time, a method for determining curcumin by high-performance thin-layer chromatography in bouillon cubes "Gallina Blanca" was proposed. Detection was carried out by densitometric scanning using CAMAG equipment when measuring absorbance at a wavelength 265 nm. The method is based on determining the peak area of curcumin in the chromatogram depending on its concentration. Curcumin content is determined by the calibration curve. The developed method was validated by the following tests: specificity, linearity, accuracy and detection limit. The calibration curve is linear in the range of curcumin concentrations from 120 to 520 ng/spot, the detection limit is 65 ng/spot. The specificity of the method is based on the ability to unambiguously evaluate the analyte in the presence of other components and is confirmed by using an external standard. The spots on the chromatograms of the test solution and the reference solution coincide in the value of Rf, which confirms the specificity of the method. Accuracy was evaluated according to the results of the analysis of various samples. The requirement for statistical insignificance of systematic error is fulfilled. The proposed method is express, characterized by satisfactory metrological characteristics, ease of implementation. Key words: high-performance thin-layer chromatography, curcumin, validation.

A review of analytical methods for the determination of herbicide residues from the group of nitrodiphenyl ethers in the environment and agricultural products, developed and validated a method for oxyfluorfen determination in soybean grain and oil method GC/ECD, which meets regulatory requirements and solves the problem of toxicant control in the above matrices. The developed method uses modern methods of sample preparation, identification and quantification of oxyfluorfen (selective extraction, capillary gas-liquid chromatography (GC/ECD GC/MS). The method is validated according to EU requirements) (SANTE/12682/2019 quality control and analytical validation procedures for pesticide residues analysis in food and feed). Key words: gas-liquid chromatography, mass spectrometry, herbicides, nitrodiphenyl ethers, control, residual amounts.