Aedes aegypti is a vector of Dengue Hemorrhagic Fever (DHF). DHF is transmitted through the bite of the Aedes aegypti mosquito which carries the virus in its body. Mosquito eradication can use natural insecticides derived from natural agent such as plants metabolites that are toxic to insects. Kayu Jawa leaf (Lannea coromandelica) is a wild plant that is easy to find. Kayu jawa leaves contain secondary metabolites such as saponins, flavonoids, polyphenols and tannins which has insecticide activity. This research was an experimental test using kayu jawa leaves (L. coromandelica) decoction at 20%, 40%, 60% and 100% to eradicate A. aegypti. Test results show that 100 % concentration of L. coromandelica leaves decoction has the greatest activity to eradicate A. aegypti. The lowest activity shown in 20% concentration. This is due to the performance of secondary metabolite compounds from L. coromandelica leaves. The conclusion of this research is L. coromandelica has an activity as an A. aegyti insecticides.

Banana peels contains simple sugars and complex sugars which can be utilized in metabolism of microorganisms. This means that banana peels can be used as an alternative culture medium for microorganisms. Rhizopus sp. and Saccharomyces cerevisiae is a type of microbe that often found in nature, and is one type of microbe that can be used in the food and non-food industries. In general, fungi need nitrogn, carbon, vitamins and growth factors. In this research, two types of fungi were grown on the Musa paradisiaca peels (pisang tanduk) and Musa acuminata peels (pisang kepok), as well as potato dextrose agar as a control medium. Growth was observed 24, 48 and 72 hours after inoculation. The data obtained is a qualitative data and analyzed descriptively. We found that the growth results on alternative medium using M. acuminata were quite good for Rhizopus sp. and S. cerevisiae. Meanwhile, medium using M. paradisiaca more suitable for the growth of Rhizopus sp. Keywords: Musa paradisiaca peels, Musa acuminata peels, Rhizopus sp., Saccharomyces cerivisiae

Blood transfusion is the transfer of blood from one person (donor) into the vein of another person (recipient). Blood components include cellular and non-cellular components. The results of the study were conducted to determine the differences in the results of 36-hour and 48-hour FFP (Fresh Frozen Plasma) samples stored at room temperature against APTT (Activated Partial Thromboplastin Time) examination. The sample was FPP (Fress Frozen Plasma) from Dr. Sotomo Hospital Surabaya, which obtained significant results, namely there was a difference in the plasma APTT value with a delay of 36 hours, plasma with a delay of 48 hours with plasma with a delay of 30 minutes or without delay, while the plasma APTT value with a 30-minute delay as a control for the normal APTT value. The Mann-Whitney statistical test showed that there was a significant difference (p<0.05) between 30-minute plasma with 36-hour delay plasma p=0.00 and 48-hour delay plasma with p=0.000. The conclusion in the hematological examination, especially the APTT examination, if there is a delay in the examination, it should be examined no more than two hours. Keywords: Fress Frozen Plasma, Activated Partial Thromboplastin Time

HbA1C is glycosylated hemoglobin at the N-terminal group, HbA1C is hemoglobin with a minor component closely related to blood glucose. HbA1C is referred to as the breakdown of sugar in the body's metabolism or glycosylated hemoglobin or glycohemoglobin. HbA1C has several examination methods such as the Boronate Affinity method and Nephelometry Photometry. The two main concepts of HbA1C measurement are based on the separation of hemoglobin fractions and chemical reactions. Both of these methods offer a fast, simple, precise and accurate alternative to the currently used methods and provide substantial freedom from many common hassles. This type of research is comparative analytic experimental. In this study, 25 samples were used during the November-January 2023 period. The purpose of this study was to evaluate the results of the HbA1C examination using two methods. In this study it was concluded that based on the Independent T Test it showed the results of Sig 0.913 > ? (0.05) so that it could be concluded that there was no significant difference in the results of the two examination methods Keywords : HbA1C, Boronate Affinity, Nephelometry Photometry

Phlebotomy is the process of drawing blood from the circulatory system through a puncture to obtain a sample. Venous blood collection is done using a tourniquet as a damming device that serves to see the vein under the skin tissue. In adults, one of the veins used is the cubital fossa and in infants using the superficial jugular vein or in the superior sagittal sinus. Platelets are disk-shaped cells with a diameter of 2-5 mm. Under normal circumstances, the number of platelets is about 150,000-450,000 / ml of blood and its life span is about 1- 2 weeks or about 8 days. Platelets are made of phospholipid substances that are important for clotting, maintaining the integrity of blood vessels and repairing damaged small blood vessels. The purpose of this study was to determine the comparison of platelet levels in vein damming at 1 minute and 2 minutes. This study was conducted in the MM2100 industrial area, samples obtained from employees who did Medical Check Up and obtained as many as 30 respondents and 60 samples. The results showed that employees in the MM2100 industrial area with average platelet levels of 274,133/µl of blood at 1 minute and average platelet levels of 278,266/µl of blood at 2 minutes. This study uses the Paired Sample T-Test method, the results obtained with a sig value of 0.229 which means there is no difference in the damming time of 1 minute and 2 minutes on platelet levels.

The microscopic examination of BTA with sputum samples aims to detect the onset and monitoring of treatment in tuberculosis. The purpose of this study is to find out the Analysis of the application of six preanalytic and analytical indicators to the quality of acid-resistant basil preparation using the Ziehl-Neelsen method at the East Jakarta Ciracas Subdistrict Health Center, which was conducted in April-June 2023. The type and design of the study are analytical cross-sectional, data analyzed using the Chi-Square test. BTA prepared quality results by performing six indicators, proportionally greater good prepared quality than poor prepared quality with 90% specimen quality percentage, 95% preparation quality, 85% preparation cleanliness, 90% preparation size, 92.5%, and 92.5% preparation accuracy. An analysis of the application of six pre-analytic and analytic indicators to the quality of the acid-resistant basil supply using the Ziehl-Neelsen method at the East Jakarta Ciracas Subdistrict Health Center which includes specimen quality, preparation cleanliness, preparation thickness, and preparation accuracy showed a meaningful relationship. The coloring of the preparedness and the size of the preparedness indicates no meaningful relationship.

Since specimen collection, preanalytical factors such as time and temperature could affect the peripheral blood smear morphology of erythrocytes, leukocytes, and thrombocytes. Delays are often caused by the limited number of medical personnel so work is not properly organized. This research is an observational qualitative descriptive study to determine the morphology of blood smears immediately checked and delay 24 hours at 2-8 ?. This study aims to determine the evaluation of K3EDTA venous peripheral blood smears immediately checked and delayed 24 hours at 2-8?. From 25 K3EDTA blood samples of Maarif Hasyim Latif Sidoarjo University students, it was found that there were no changes in the morphology of erythrocytes and platelets. Meanwhile, in leukocyte cells, changes occur in the neutrophil segment and stab granules, which is the granules fade, and the leukocyte nuclei can still be identified properly, so that do not affect the examination results. In conclusion, the Evaluation of the K3EDTA Peripheral Blood Smear was immediately checked and delay 24 Hours at the temperature of 2-8 ? has no change, which is still within normal limits. Keywords : Blood Smear, Delayed, K3EDTA, Immediately checked

Soil is a mineral material that has several unconsolidated forms which are located on the surface of the earth and have undergone several treatments. Soil-Transmitted Helminth (STH) is an intestinal nematode that in its life cycle requires a soil medium for the infective form to mature. worms are moist soil, shady, and protected from sunlight. This study aims to determine whether there is contamination of "Soil-Transmitted Helminth" eggs and what species are present in the rice fields using the Native method. This examination uses a 2% eosin solution. The sample uses paddy fields in Sukodono Village. Sampling at 3 sampling points with 3 types of samples, the total sample is 9 samples of paddy soil. The research was conducted on April 26-28 2021 at the Parasitology Laboratory, Faculty of Health Sciences, Maarif Hasyim Latif University, Sidoarjo, on 9 samples of paddy soil, the results obtained were 3 types of samples, or 33% of the soil contaminated with worm eggs and 6 types of samples or 66% not contaminated with worm eggs. Keywords: Paddy fields, Soil Transmitted Helminth, Native Method

Comorbid diabetes mellitus (DM) in pulmonary tuberculosis (TB) patients may increase the risk of death even with or during TB treatment. Immunocompromised conditions due to DM increase the risk of infection and sputum conversion failure. The risk of recurrence in comorbid patient is also higher after treatment. Objective of this research is to describe the comparison of further microscopic examination of pulmonary tuberculosis patients without DM and pulmonary tuberculosis patients with a history of diabetes at the Kedundung Public Health Center. The study was conducted by observing each of 15 TB positive sputum samples accompanied by a history of DM and those without any history of DM 2 months post-treatment on further microscopic examination. Further microscopic examination showed that 100% of sputum samples of TB patients without a history of DM and receiving treatment gave negative results, while sputum samples of TB patients with DM history and receiving treatment, while sputum samples of patients who had received treatment showed results of 2+ by 80% and 3+ by 20% and there were no negative results for TB. These results prove that there is an effect of a history of DM disease on TB treatment outcomes. Keywords : Further microscopic examination, Pulmonary tuberculosis (TB), Diabetes Mellitus (DM)

Hematocrit is the total volume of red blood cells in 100 ml of blood expressed as a percentage of blood volume. Hematocrit examination is needed to help diagnose diseases such as anemia and dengue hemorrhagic fever (DHF) to determine the total volume of red blood cells or erythrocytes. The normal value for hematocrit examination is 40-54% for males and 37-47% for females. The purpose of this study was to determine the effect of variations in centrifugation time on hematocrit values using the microhematocrit method. This type of research is quantitative using experimental method with cross sectional design. The method used for hematocrit examination is the microhematocrit method with variations in centrifugation time of 2 minutes, 3 minutes, 4 minutes, and 5 minutes as a control with a speed of 12,000 rpm. The respondents of this study were 35 respondents and the samples of this study were 35 venous blood samples taken from Binawan University students, Medical Laboratory Technology Study Program batch 2021 and 2022. The average result of the 2-minute centrifugation hematocrit value examination is 37.87%, 3-minute centrifugation is 37.57%, 4-minute centrifugation is 37.30%, and 5-minute centrifugation is 37.25%. The Wilcoxon test results show the Sig. (2-tailed) of 2-minute centrifugation compared with 5 minutes is 0.00 (<0.05), 3-minute centrifugation compared with 5 minutes is 0.001 (<0.05), 4-minute centrifugation compared with 5 minutes is 0.560 (>0.05). The results of this study showed that hematocrit values with centrifugation times of 2 minutes and 3 minutes compared to 5 minutes had significant differences in hematocrit values.