The purpose of this study was to describe an atypical and severe case of giant cell arteritis (GCA). This was a case report description. The authors report the case of a 76-year-old man who presented with severe and progressive bilateral visual loss. On presentation at the ophthalmology emergency room, the patient's right eye (OD) displayed aqueous flare, hyphema, rubeosis iridis , and dense posterior capsular opacification. After YAG laser capsulotomy, vitreous densifications, intraretinal hemorrhages, cotton wool spots, retinal ischemia, and vasculitis were observed in the fundoscopy. The patient's left eye (OS) also presented intraretinal hemorrhages and cotton wool spots around the temporal arcades. The diagnostic workup excluded infectious diseases, demyelinating diseases, and ocular ischemic syndrome due to carotid obstruction. Proteinogram revealed a monoclonal gammopathy, suggesting a possible hematologic condition. High-dose corticotherapy was initiated, which improved the vitreous densifications and enabled the visualization of the pale optic disk. The remaining study did not confirm the diagnosis of hematologic disease. During follow-up, bilateral VA deteriorated, with the development of progressive pallor in the OS optic disk. Follow-up fluorescein angiography demonstrated progressive retinal and choroidal ischemia. Finally, owing to high clinical suspicion, temporal artery Doppler ultrasound was performed, confirming the diagnosis of GCA. GCA may present multiple ocular features. The knowledge of these different presentations, including retinal and choroidal ischemia or uveitis, is critical for timely diagnosis and treatment initiation. Since patients with GCA often present with vision loss, ophthalmologists may be the first medical doctors who contact with these patients, being on the frontline of GCA diagnosis.

Predictors of macroeconomic indicators rely primarily on traditional data sourced from National Statistical Offices. However, new data sources made available from recent technological advancements, namely data from online activities, have the potential to bring about fresh perspectives on monitoring economic activities and enhance the accuracy of forecasting. This paper reviews the literature on predicting macroeconomic indicators, such as the gross domestic product, unemployment rate, consumer price index or private consumption, based on online activity data sourced from Google Trends, Twitter (rebranded to X) and mobile devices. Based on a systematic search of publications indexed on the Web of Science and Scopus databases, the analysis of a final set of 56 publications covers the publication history of the data sources, the methods used to model the data and the predictive accuracy of information from such data sources. The paper also discusses the limitations and challenges of using online activity data for macroeconomic predictions. The review concludes that online activity data can be a valuable source of information for predicting macroeconomic indicators. However, one must consider certain limitations and challenges to improve the models’ accuracy and reliability.

ABSTRACT Olive oil production and consumption has been increasing due to its organoleptic properties and health benefits. Consequently, waste generation also raised leading to environmental concerns. Nevertheless, this waste contains high amounts of bioactive compounds with potential to be extracted, recovered, and applied, following circular economy policies. New extractive technologies, especially green ones, are important for the success of this venture. This review focuses on the olive oil production chain, from the tree to olive oil and the respective waste/by-products, presenting and discussing emergent and green methodologies to recover and give new life to bioactive compounds that are usually discarded. Oleuropein, hydroxytyrosol, and other related compounds are molecules with important biological activities. They can be recovered in high concentrations through such technologies and used successfully in different industries to meet the sustainability and circular economy principles of the chain.

Staphylococcal enterotoxin A (SEA) is the most frequently reported in staphylococcal food poisoning (SFP) outbreaks. Aptamers are single-stranded nucleic acids that are seen as promising alternatives to antibodies in several areas, including diagnostics. In this work, systematic evolution of ligands by exponential enrichment (SELEX) was used to select DNA aptamers against SEA. The SELEX protocol employed magnetic beads as an immobilization matrix for the target molecule and real-time quantitative PCR (qPCR) for monitoring and optimizing sequence enrichment. After 10 selection cycles, the ssDNA pool with the highest affinity was sequenced by next generation sequencing (NGS). Approximately 3 million aptamer candidates were identified, and the most representative cluster sequences were selected for further characterization. The aptamer with the highest affinity showed an experimental dissociation constant (KD) of 13.36 ± 18.62 nM. Increased temperature negatively affected the affinity of the aptamer for the target. Application of the selected aptamers in a lateral flow assay demonstrated their functionality in detecting samples containing 100 ng SEA, the minimum amount capable of causing food poisoning. Overall, the applicability of DNA aptamers in SEA recognition was demonstrated and characterized under different conditions, paving the way for the development of diagnostic tools.

AbstractNETmix is a network of mixing chambers interconnected by channels, in which the most efficient mixing occurs under chaotic flow regimes. Chaos is highly influenced by the topology, particularly by the ratio chamber diameter/channel width, D/d. In this work, micromixing in NETmix is characterized through Lagrangian Mixing Simulation (LMS), which enables to establish the relation between the degree of mixing and the topology, D/d. The degree of mixing is then related with the Eulerian chaoticity from hydrodynamics and spectral analysis. The best mixing is obtained at D/d = 6.65 and is associated to a thin 8 shaped strange attractor. The spectral analysis shows a principal oscillation frequency at St = 0.13. Since the quasi‐periodic regime is alternated with chaotic behavior, NETmix has an intermittent bifurcation. For the NETmix system, orderly flows in the quasi‐periodic regime have higher mixing intensity than less orderly flows in the weak or strong chaotic regimes.

The diagnosis of a child’s complex chronic illness may impact family relationships and cohesion. The Impact on Family Scale (IFS) is an instrument used to assess the parental perception of the effects of children’s chronic illness on family life. With a sample of 110 mothers and fathers between the ages of 29 and 50 who have a child with a complex chronic illness, we examine evidence of the validity of the IFS for use in Portugal within this specific family configuration, (1) comparing its factor structure with the original one; (2) assessing its reliability; and (3) evaluating its relationship with life satisfaction and family cohesion/acceptance. As expected, CFA analysis showed that IFS is a one-factor reliable instrument with 12 items (Cronbach’s alpha = 0.910), which are negatively correlated with satisfaction with life (r = −0.229, p = 0.016) and positively correlated with family acceptance and cohesion (r = 0.363; p < 0.001). The results support the validity of the IFS in families with children and adolescents with a complex chronic illness. The implications of the use of this instrument for research and professional practice is analyzed.

In this study, we quantified relevant biophysical characteristics of two elite rowers across a wide range of intensities. Two <40-year-old male and female Olympic and World Championship finalists performed a 7 × 3 min protocol plus 1 min maximal effort on a rowing ergometer. The intensity increase resulted in maximum values of 79.4 ± 2.4 and 69.7 ± 1.5 mL/min/kg for oxygen uptake, 179.3 ± 5.7 and 152.5 ± 2.9 L/min for ventilation, 170 ± 1 and 173 ± 0 bpm for heart rate, 10.6 and 15.8 mmol/L for blood lactate concentration, and 38.1 ± 0.03 and 38.8 ± 0.03 °C for core temperature for the male and female rowers. The percentage of power corresponding to a previously conducted maximum 2000 m rowing ergometer test and the work at each step increased from 49 to 127 and 42 to 103% and from 226.8 to 398.9 J and 174.0 to 250.0 J, from low to extreme intensities, for the male and female. Concurrently, there was a decrease in cycle length and propulsive time, followed by an increase in maximal handle drive velocity, with the rise in rowing intensity. These world-class rowers seem capable of maintaining physiological and technical profiles (and a remarkable capacity to generate substantial power) at this phase of their careers possibly due to long-term engagement in elite-level training. Biophysical data provide valuable referential information for guiding rowers to improve their performance.

Glucose-derived carbon hybrids were synthesized by hydrothermal treatment in the presence of oxidized carbon nanotubes. Additionally, iron and nitrogen functionalities were incorporated into the carbon structure using different methodologies. The introduction of iron and nitrogen in a single step under a H2 atmosphere favored the formation of quaternary nitrogen and oxidized nitrogen, whereas the incorporation of nitrogen under an N2 atmosphere after doping the hybrids with iron mainly produced pyridinic nitrogen. The samples were characterized by scanning electron microscopy, X-ray spectroscopy, adsorption isotherms, inductively coupled plasma optical emission spectrometry, and Raman spectroscopy. The presence of iron and nitrogen in the carbons increases the onset potential toward oxygen reduction in KOH 0.1 mol L−1 by 130 mV (0.83 V), in comparison to carbonized glucose, whereas the reaction mechanism shifts closer to a direct pathway and the formation of HO2− decreases to 25% (3.5 electrons). The reaction rate also increased in comparison to the carbonized glucose, as observed by the decrease in the Tafel slope value from 117 to 61 mV dec−1. Furthermore, the incorporation of iron and nitrogen in a single step enhanced the short-term performance of the prepared electrocatalysts, which may also be due to the higher relative amount of quaternary nitrogen.

Both music and language are found in all known human societies, yet no studies have compared similarities and differences between song, speech, and instrumental music on a global scale. In this Registered Report, we analyzed two global datasets: (i) 300 annotated audio recordings representing matched sets of traditional songs, recited lyrics, conversational speech, and instrumental melodies from our 75 coauthors speaking 55 languages; and (ii) 418 previously published adult-directed song and speech recordings from 209 individuals speaking 16 languages. Of our six preregistered predictions, five were strongly supported: Relative to speech, songs use (i) higher pitch, (ii) slower temporal rate, and (iii) more stable pitches, while both songs and speech used similar (iv) pitch interval size and (v) timbral brightness. Exploratory analyses suggest that features vary along a "musi-linguistic" continuum when including instrumental melodies and recited lyrics. Our study provides strong empirical evidence of cross-cultural regularities in music and speech.

Human papillomavirus (HPV) is the most prevalent sexually transmitted infection among young women. Notably, more than ten years after the introduction of HPV vaccination programs in Europe, it is essential to review the real-world evidence of the incidence of anogenital warts (GWs) among women vaccinated during childhood. In this systematic review, three databases were searched for studies published between January 2008 and September 2023. Nine cohort studies were included. A total of 890,320 HPV-vaccinated women and 1,922,033 unvaccinated women were evaluated. All the studies but one investigated the 4vHPV vaccine. The incidence rate of GWs in vaccinated women ranged from 0.0 to 1650 per 100,000 person-years. The highest incidence rates were found in women vaccinated with one dose at the age of 17-19 years old and in fully vaccinated women only after 19 years of age. Similar incidence values were reported among unvaccinated women. The incidence of GWs was lower when the age at first dose was 9-11 years old. This systematic review reveals that the incidence of GWs among HPV-vaccinated women is related to the age of vaccination and the number of vaccine doses received. In the post-vaccination era, epidemiological surveillance of the incidence of GWs and their genotypes is crucial.