El texto defiende la importancia de reconocer el mundo de los videojuegos como un espacio creativo para la mediación cultural y artística. Asimismo, destaca la urgencia de implementar programas de formación inicial y continua que capaciten al profesorado para comprender, valorar y disfrutar del videojuego en toda su complejidad.

Chronic myeloid leukemia (CML) is a type of leukemia characterized by myeloid proliferation. Morgana is a chaperone protein, whose levels are lower due to the overexpression of miR-15a in CP phase. By quantitatively detecting miR-15a and Morgana, we could verify the correlation of them to CP-CML, thus as biomarkers of CP-CML diagnosis.

Los sistemas alimentarios locales y los productos agroalimentarios de proximidad se definen por su enfoque en la sostenibilidad y la reducción de la distancia entre productores y consumidores finales, los cuales impulsan la demanda de alimentos frescos y de origen local. El desarrollo y fomento de tales sistemas se aprecia en instrumentos internacionales, como el Pacto de Milán de 2015, o en políticas a nivel de la UE como la PAC. Ciertamente la PAC, en sus versiones 2014/2020 y 2023/2027, ha impactado positivamente en la producción agroalimentaria local y en las cadenas cortas de distribución en España. También la legislación autonómica española, y como ejemplo la Ley de la Huerta Valenciana de 2018, protege las tierras agrícolas y promueve la producción agraria local y periurbana, luchando contra el abandono de fincas agrícolas y fomentando la función social de la propiedad del suelo rústico, en aras de la sostenibilidad del territorio rural.

The aim of this study was to build on the work already conducted in optimising lumpfish broodstock temperature, photoperiod and nutrition by providing vital information on the stress relationship between mucosal barrier functions and the sexual development of lumpfish broodstock. From a population of approx. 300 fish of 1587 g (SEM ± 704 g), a sub-population of 20 fish was injected with 30 mg/kg fish cortisol implants on 5 January 2018. The control group was not treated with implants. The stress-induced cortisol implant group showed elevated plasma cortisol over a period of approximately one month. Analyses of mucus cell area and density suggested that induced stress could produce a positive functional response (stimulus) in lumpfish early in the experiment through a gentle increase in the barrier strength of the skin, reduced mucus cell size and increased density of mucous cells, resulting in a strengthening of the respiratory capacity of the gills. Mucous cell density and calculated barrier strength in the skin were significantly negatively correlated with plasma cortisol, while in the gill filaments of females, there was a significant negative correlation between mucous cell density and oestrogen levels. The reduced density of “empty” cells (Q cells) after stress induction indicates that these cells are important for the maintenance of homeostasis (physiological equilibrium). The fish in this experiment were considered more robust compared to previous analyses on smaller lumpfish.

In response to an everlasting housing crisis, cities worldwide have witnessed a surge in alternative housing initiatives (AHIs) driven by third‐sector organisations. In Brussels, a network of third‐sector organisations has been developing strategies with each other and local authorities, resulting in a plethora of initiatives focusing on various critical situations. Drawing on ethnographic research in a Brussels AHI, this article investigates how its complex multi‐actor structure affects the daily life of its inhabitants both within their dwellings and the wider neighbourhood. By capturing the tactics employed by third‐sector actors on the ground, which often deviate from their initial strategy for reclaiming the right to housing, as well as the homing practices of the inhabitants, the article focuses on tracing how and why such a housing configuration does or does not address its inhabitants’ interplaying vulnerabilities related with the housing crisis as well as their relationship with the local urban fabric.

AbstractGene augmentation therapy is a promising treatment for incurable, blinding inherited retinal diseases, and intravitreal delivery is being studied as a safe alternative to subretinal injections. Adeno-Associated Viruses (AAV) are commonly-used vectors for ocular gene augmentation therapy. Naturally occurring pre-operative exposure and infection with AAV could result in presence of neutralizing antibodies (NAB’s) in patients’ serum, and may affect the safety and efficacy of treatment. Our aim was to characterize the humoral response against AAV pre- and post-intravitreal delivery of AAV2.7m8 vectors in a naturally-occurring sheep model of CNGA3 achromatopsia. Serial serum neutralization assays were performed to screen sheep for pre-exiting anti-AAV2 NAB’s, and to assess the effect of intravitreal AAV2.7m8 injection on post-operative NAB titers and intraocular inflammation in sheep. The effect of viral dose and transgene type were also assessed. Serological screening revealed pre-operative seropositivity in 21.4% of animals, with age being a risk factor for the presence of anti-AAV2 NAB’s. NAB titers increased following intravitreal AAV administration in the majority of sheep. There was no significant difference in the degree of post-operative serum neutralization between pre-operatively seronegative sheep and those with pre-existing antibodies. However, only sheep with pre-existing antibodies presented with signs of post-operative inflammation. We conclude that pre-existing anti-AAV2 NAB’s do not affect the level of post-operative NAB titers; however, they increase the risk of post-operative ocular inflammation. Our results could have implications for the management of AAV-mediated ocular gene therapies, a technology being increasingly studied and used in patients.