In this paper we use the case of Nepal to advance political geographic analyses of how, during moments of rupture, territory act as an important political technology in state restructuring, and how urban demarcation along with other territorial structures of the state will play a significant role in this process. Nepal has experienced more than three decades of state-restructuring characterized by consecutive political and constitutional crises, including close to ten years of violent conflict. Within the brief period between 2014 and 2017, more than 230 new municipalities were demarcated on top of the existing 58. In our analysis we unpack why and how the number of municipalities is quadrupled at that particular moment of time and how this is shaped by and have implications for re-configurations of Nepal's territorial structures. This is achieved through a historical analysis of how the state's politico-administrative system has been mapped, reasoned and challenged. The analysis is based on official documents, such as census data and reports, legislative acts, public debates and academic analyses of processes of administrative and political reforms and conflicts in Nepal since the early 1990s. It therefore engages with a rich literature on conflict, the post-conflict situation and the restructuring of the state. Based on our findings we argue that urban demarcation is an important part of a states' political technology complex, and warn against trends in studies of urbanization to question the analytical bearing of differentiating the rural and urban. Showing that Nepal's recent urban boundaries have been justified by the need to achieve a better geographical balance, we conclude by arguing for the need for studies of urban transformations that critically examine whether and how the new territorial structures in their implementation contribute to balance geographical and social inequalities.

Abstract Background: Pseudomonas aeruginosa is an opportunistic human pathogen and are reported to cause acute and chronic infectious diseases. Due to its high ability to acquire resistance to many antibiotics, it has become a global public health threat. It consists of some virulence genes that may lead to its pathogenicity. The main objective of this cross-sectional study was to detect the virulence genes and antibiotic susceptibility pattern of P. aeruginosa isolated from clinical specimens collected from governmental hospital of Nepal.Methods: A total of 7898 clinical specimens were analyzed for the period of six months from November 2018 to April 2019. The specimens were cultured on Nutrient agar, Blood agar, MacConkey agar, Chocolate agar, Cysteine-Lactose, Electrolyte Deficient agar plates and were incubated at 37°C for 24 hours. All the isolates were identified by standard biochemical tests and further confirmed by growth on Cetrimide agar plate. The antibiotic susceptibility testing was performed by modified Kirby-Bauer disc diffusion method following CLSI guideline. Multiplex-PCR was done to detect the virulence genes oprL and toxA. Statistical analysis was carried out using IBM SPSS Statistic ver. 25 and the p-value was calculated at significance level (0.05%) by using Chi square.Results: Out of these specimens investigated, 87 isolates were tentatively identified to be P. aeruginosa in which 20 (22.98 %) were found to be multidrug resistant. Comparatively, most of the P. aeruginosa were isolated from outpatients 63 (72.41 %) than inpatients 24 (27.58 %), from male 56 (64.36 %) than female 31 (35.63 %) and in age group 60-79 years (41.37 %). AST result showed the highest resistance of 100% with cefixime whereas susceptibilities of 83.9% and 81.6% with polymixin B and tobramycin were noticed respectively. The PCR results showed that all P. aeruginosa isolates carried oprL 87 (100%) and 83 (95.4 %) isolates showed toxA genes. Conclusion: The studies revealed that almost all P. aeruginosa harbors both oprL and toxA genes.

The combination of SCXRD and atomic PDF is a potential tool for the standardization of atomic scale structures of nanomaterials. This article describes the essential approach and application to ZnS polymorphs.

Zn-ferrite nanoparticles were synthesized at room temperature by mechanical alloying the stoichiometric (1:1 mol%) mixture of ZnO and α-Fe2O3 powder under open air. Formation of both normal and inverse spinel ferrite phases was noticed after 30 minutes and 2.5 hours ball milling respectively and the content of inverse spinel phase increased with increasing milling time. The phase transformation kinetics towards formation of ferrite phases and microstructure characterization of ball milled ZnFe2O4 phases was primarily investigated by X-ray powder diffraction pattern analysis. The relative phase abundances of different phases, crystallite size, r.m.s. strain, lattice parameter change etc. were estimated from the Rietveld powder structure refinement analysis of XRD data. Positron annihilation lifetime spectra of all ball milled samples were deconvoluted with three lifetime parameters and their variation with milling time duration was explained with microstructural changes and formation of different phases with increase of milling time duration.