Zur Fraktionierung der löslichen Ribonucleinsäure

Abstract

On the fractionation of soluble ribonucleic acid Brewer's yeast soluble RNA was subjected to a 200-transfer counter-current distribution in the tri- n-butylamine-containing solvent system. 16 ★★ ★★ Siehe Anmerkung bei der Korrektur amino acids were incorporated into the fractions using an aminoacyl soluble RNA synthetase from yeast, 10 of them also with a synthetase from rat liver. Acceptor activity curves with two and three peaks were found for several amino acids. Some curves are similar to the ones obtained with the solvent systems of Holley and of Kirby, Doctor and Connelly. Differences in the distribution patterns (permitting a useful combination of the solvent systems) were found particularly in the alanine, lysine, tyrosine and serine transfer RNA's. Chromatography on methylated albumin on kieselgur columns of the two phenylalanine and the three valine transfer RNA's separated by counter-current distribution indicated that these transfer RNA's are also present in unfractionated soluble RNA and are not formed during the separations. Phenylalanine transfer in the Escherichia coli ribosomal system was stimulated by poly-U in both of the phenylalanine transfer RNA's; the extent of stimulation was different for the two transfer RNA's. No stimulation of phenylalanine transfer by Poly-UC could be detected in either transfer RNA.