Abstract
The interaction of erythrocytes and [125I]insulin/insulin were studied up to a total insulin concentration of 409 mumol/l. Assuming a single class receptor model the evaluation of receptor affinity Ka and concentration R0 may be performed either by non-linear regression analyses with iteration procedures of R0, Ka and U (nonspecific binding), or by a linear regression analysis of the initial part of the Scatchard plot. Nonlinear fitting of data to a two class receptor model gives results that are reliable only for the high affinity receptor site. The non-definable step of R0 determination leads to uncertainties in results determined by the negative cooperativity model. From the results of this investigation and from considerations of signal modulation by receptor occupancy, some recommendations have been formulated for the evaluation of binding parameters; these should contribute to an improvement in the comparability of studies on the interactions of erythrocytes and insulin.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
