Abstract
ZOOSPORES were obtained from the sporangia of the California strain1,2of Plasmopara viticola (B. and C.) Berl. and De T. growing in Vitis californica Benth. and V. girdiana Munson leaves. Germination occurred in distilled water at 10°–16° C. Active suspensions of zoospores were fixed by exposure for 30 sec. to the vapour of a 2 per cent osmic acid solution. Fixation darkened the flagella and permitted them to be measured easily under the light microscope. For electron microscopy the zoospore suspension was fixed, as above, on a ‘Parlodion’ film covering a 200-mesh electron microscope screen and allowed to dry.
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