Zoonotic Rickettsia Species in Small Ruminant Ticks From Tunisia.

Hanène Belkahia,Mourad Ben Said,Sayed Zamiti,Rachid Selmi,Lilia Messadi,Monia Daaloul-Jedidi

doi:10.3389/fvets.2021.676896

Hanène Belkahia, Mourad Ben Said + Show 4 more

Open Access

https://doi.org/10.3389/fvets.2021.676896

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Abstract

Tick-borne rickettsioses present a significant public health threat among emerging tick-borne diseases. In Tunisia, little is known about tick-borne Rickettsia pathogens. Therefore, the aim of this study was to investigate the presence of Rickettsia species in small ruminant ticks from Tunisia. Adult ticks (n = 694) were collected from goats and sheep in northern Tunisia. Obtained ticks were identified as Rhipicephalus turanicus (n = 434) and Rhipicephalus sanguineus sensu lato (n = 260). Selected ticks (n = 666) were screened for the presence of Rickettsia spp. by PCR targeting a partial sequence of the ompB gene followed by sequence analysis. Rickettsial DNA was detected in 122 (18.3%) tested tick samples. The infection rates in Rh. turanicus and Rh. sanguineus s.l. ticks were 23.4 and 9.5%, respectively. The overall prevalence of rickettsial DNA was markedly higher in ticks collected from goats (23.2%) compared to those infesting sheep (7.9%). The detection of rickettsial DNA was significantly higher in ticks from the governorate of Beja (39.0%) than those from the governorate of Bizerte (13.9%). Two additional genes, the outer membrane protein A gene (ompA) and the citrate synthase gene (gltA), were also targeted for further characterization of the detected Rickettsia species. Genotyping and phylogenetic analysis based on partial sequences (n = 106) of the three different genes revealed that positive ticks are infected with different isolates of two Spotted Fever Group (SFG) Rickettsia, namely, Rickettsia massiliae and Rickettsia monacensis, closely related to those infecting camels and associated ticks from Tunisia, and humans and small ruminant ticks from neighboring countries like Italy, France, and Spain.

Highlights

Rickettsia species are included into four groups: the spotted fever group (SFG) rickettsiae, the typhus group, the Rickettsia bellii group, and the Rickettsia canadensis group [1]
To better understand the epidemiology of Rickettsia species in Tunisia, we investigated, in the present molecular survey the occurrence of rickettsial bacteria in small ruminant ticks according to potential risk factors
Almost all specimens were removed from animals located in the governorate of Bizerte (82%) while ticks collected from small ruminants in El Alia were the most numerous (43.5%) compared to those in other localities (Figure 1 and Table 2)

Summary

Introduction

Rickettsia species (family Rickettsiaceae; order Rickettsiales) are included into four groups: the spotted fever group (SFG) rickettsiae, the typhus group, the Rickettsia bellii group, and the Rickettsia canadensis group [1]. These pathogens infected several domesticated and wild vertebrate hosts through hematophagous arthropod vectors bites (mainly ticks, fleas, and mites). Spotted fever group rickettsioses (SFG) are considered as emerging and reemerging diseases affecting animals worldwide. They are caused by the pathogenic and zoonotic spotted fever Rickettsia bacteria mainly transmitted by ticks. Humans may be accidently infected especially in tropical areas [1, 2]

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