Abstract
Rotavirus was recognized as the virus that responsible for causing acute gastroenteritis, especially young livestock. Taiwan Center for Disease Control (CDC) confirms the majority cases of acute gastroenteritis in Taiwan on February 2015 were caused by rotavirus. In this study, we report the incidence and zoonotic impact of rotavirus strain from Taiwan. This study examined 90 (swine) and 60 (bovine) fecal samples collected from south of Taiwan in March 2015. Detection of rotavirus using VP6 gene by RT-PCR technique with amplicons 379 bp. Zoonotic potential analysis based on nucleotide sequence and phylogenetic analysis. RT-PCR utilizing the primers specific for VP6 gene detected rotavirus with positive reactions 3/30 (10%) in piglets and 1/20 (5%) in the calf. Based on the nucleotide sequences and phylogenetic analysis indicated that 1 of 3 wild strains from swine rotavirus had 85.0% - 91.1% and 1 wild strain from bovine had 78.7% - 85.9% identity relations with human strains. These findings indicated that the wild strains of swine and bovine rotavirus may broadly spread and contribute to zoonotic transmission. Keywords: Bovine, Rotavirus, RT-PCR, Swine, Zoonotic.
Highlights
Rotavirus is enteric pathogen causing acute watery diarrhea in young man and various animal species
Rotavirus is belongs to family reoviridae, icosahedral in structure, 60-80 nm in diameter, non enveloped, possess a one, two or three layered capsid, and containing a genome of 11 segements of double stranded RNA encoding six structural (VP1, VP2, VP3, VP4, VP6, and VP7) and five or six nonstructural (NSP 1-6) proteins [3]
There are eight species of rotavirus, referred to as rotavirus A, rotavirus B, rotavirus C, rotavirus D, F, and G, rotavirus E, rotavirus H [5], rotavirus H was tentatively assigned to a novel rotavirus species [6]
Summary
Rotavirus is enteric pathogen causing acute watery diarrhea in young man and various animal species. Rotavirus is belongs to family reoviridae, icosahedral in structure, 60-80 nm in diameter, non enveloped, possess a one, two or three layered capsid, and containing a genome of 11 segements of double stranded RNA (dsRNA) encoding six structural (VP1, VP2, VP3, VP4, VP6, and VP7) and five or six nonstructural (NSP 1-6) proteins [3]. This virus can be transmitted by consuming contaminated food and direct contact with an infected individual or contaminated objects. Characterization of VP6 gene is encoded 379 amino acid sequences [10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
