Abstract
A rapid fluorometric method is described for the determination of lactate and cholesterol by using ZnO nanowires (ZnO NWs). The assay is based on the detection of the hydrogen peroxide generated during the enzymatic reactions of the oxidation of lactate or cholesterol. Taking advantage of the electrostatic interactions between the enzymes and the ZnO NWs, two bioconjugates were prepared by mixing the nanomaterial and the enzymes, viz. lactate oxidase (LOx) or cholesterol oxidase (ChOx). The enzymatically generated hydrogen peroxide quenches the fluorescence of the ZnO NWs, which have emission peaks at 384nm and at 520nm under 330nm photoexcitation. H2O2 quenches the 520nm band more strongly. Response is linear up to 1.9μM lactate concentration, and up to 1.1μM cholesterol concentration. Relative standard deviation was found to be 5%. The detection limits for lactate and cholesterol are 0.54 and 0.24μM, respectively. Graphical abstractSchematic representation of fluorescence assay based on ZnO nanowires photoluminiscence for lactate and colesterol detection.
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