Abstract
In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.
Highlights
The determination and monitoring of antibiotics and other pharmaceutical compounds in the human body is of great interest to human health and to analytical and clinical chemistry, to veterinary, pharmaceutical and food industries
We have successfully demonstrated the fabrication of a zinc oxide (ZnO) nanorods based biosensor with good reproducibility and selectivity for quick monitoring of penicillin with immobilization of penicillinase enzyme by simple physical adsorption method
As the amount of charges produced around the ZnO nanorods based sensor electrode changes, a change into the electrode potential was observed [39]
Summary
The determination and monitoring of antibiotics and other pharmaceutical compounds in the human body is of great interest to human health and to analytical and clinical chemistry, to veterinary, pharmaceutical and food industries It is very important during physiological and biochemical reactions occurring in the human body to know the penicillin concentration [1]. Such a method of immobilization has some disadvantages and the biosensors developed by this method have poor stability and quick stripping of enzyme molecules during the operation due to the weak adhesion of the membrane The reason for this might be due to the inter enzyme molecules cross linking instead of the binding of the enzyme molecules to the transducer surface. The potentiometric technique is easy, quick, and of low cost for the detection of penicillin by using the ion selective electrodes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
