ZnO Nanoparticles and Its Interaction With Chitosan : Profile Spectra and Their Activity Against Bacterial

Abstract

<p>The biosynthesis of ZnO nanoparticles and synthesis of chitosan- ZnO nanoparticles were studied. The aims of this research were biosynthesis of ZnO nanoparticles, synthesis of chitosan-ZnO nanoparticles, its characterization and, used as an antibacterial agent of <em>Escherichia coli</em><strong>. </strong>ZnO nanoparticles were biosynthesized by reacting with ethanolic extract of guava seeds leaves (<em>Psidium guajava </em>L.) and zinc acetate dihydrate solution. Chitosan-ZnO nanoparticles were synthesized by the heating method. ZnO nanoparticles and chitosan-ZnO nanoparticles were characterized by FTIR spectroscopy and X-ray diffraction, respectively. The suspension of chitosan-ZnO nanoparticles was used as an antibacterial agent with a paper disk method. The result showed that the Zn-O group at ZnO nanoparticles was detected at a wavenumber 615.29 and 673.16 cm<sup>-1</sup> The crystallite size of ZnO nanoparticles was 1.43 nm. The wavenumber of 617-655 cm<sup>-1</sup> is the Zn-O group at the structure of the chitosan-ZnO nanoparticle. The average of diameter inhibition zone of chitosan-ZnO nanoparticles (1:2) at concentration 0.25 and 0.5 % (w/v) to <em>Escherichia coli</em> was 15.7 ± 1.0 and 18.3 ± 0.4 mm respectively. </p>