Abstract
In this study, a new amplified signal probe of ZnIn2S4 quantum dot@TiO2 nanosphere was used to sensitize the BiOI substrate, which was combined with a unique tripod DNA walker to develop a new photoelectrochemical (PEC) biosensor for microRNA-21 (miRNA-21) assay. TiO2 has good PEC activity for its narrow band gap, and a large number of ZnIn2S4 quantum dots (QDs) were attached to the TiO2 to form a good heterojunction, realizing double enhancement of PEC performance. Moreover, a tripod DNA walker for target-cycling amplification was synthesized using a simple catalytic hairpin assembly (CHA). After DNA hairpin 5 (HP5) was linked to the BiOI-modified electrode, the prepared three-legged DNA walker opened HP5 DNA, and formed a single-stranded DNA (ssDNA). Then the ZnIn2S4 QDs@TiO2 signal probe was linked to the electrode by specific hybridization of ssDNA with the tripod walker, realizing noticeable amplification of PEC signal for sensitive detection of miRNA-21. This work combined the excellent PEC characteristic, the amplified signal of ZnIn2S4 QDs@TiO2 heterojunction, and an enzyme-free tripod walker amplification technique to achieve sensitive detection of target, which opens a new PEC analysis method for clinical application.
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