Abstract
Zinc finger with KRAB and SCAN domain 3 (ZKSCAN3) upregulates genes encoding proteins involved in cell differentiation, proliferation and apoptosis. ZKSCAN3 has been reported to be overexpressed in several human cancers such as colorectal cancer and prostate cancer and is proposed as a candidate oncoprotein. However, the molecular mechanism by which ZKSCAN3 participates in carcinogenesis is largely unknown. Here, we evaluated ZKSCAN3 expression in uterine cervical cancers (CC) by immunohistochemistry using formalin-fixed, paraffin-embedded tissues from 126 biopsy samples from 126 patients. The clinicopathological findings were analyzed and compared with ZKSCAN3 expression levels. ZKSCAN3 was strongly overexpressed in CCs compared to adjacent non-neoplastic cervical mucosa tissues. Moreover, a gene copy number assay showed amplified ZKSCAN3 in CC samples. ZKSCAN3 overexpression was also significantly associated with poor overall survival of the patients. Overall, our findings indicate that ZKSCAN3 overexpression is a frequent event in uterine CC and is correlated with a poor clinical outcome. ZKSCAN3 could be developed as a molecular marker for prognostic prediction and early detection.
Highlights
Several decades ago, uterine cervical cancer (CC) was the leading cause of cancer-related deaths in women worldwide, but ranks as the fourth-most common cause of cancer overall and the fourth-most common cause of death from cancer in women [1]
We carried out quantitative polymerase chain reaction to determine the mRNA expression expression levels of ZKSCAN3 in four cervical cancer cell lines (C33A, Caski, HeLa and SiHa), along levels of ZKSCAN3 in four cervical cancer cell lines (C33A, Caski, HeLa and SiHa), along with GM00637, with GM00637, a human fibroblast line, as a control sample
ZKSCAN3 oncoprotein,and anditsitsexpression expression frequently upregulated in human
Summary
Uterine cervical cancer (CC) was the leading cause of cancer-related deaths in women worldwide, but ranks as the fourth-most common cause of cancer overall and the fourth-most common cause of death from cancer in women [1]. Zinc finger with KRAB and SCAN domain 3 (ZKSCAN3) is a member of the KRAB and SCAN domain-containing zinc finger protein family, encoded by the ZKSCAN3 gene in chromosome 6p22.1 [3] This family of proteins is involved in cell differentiation, cell proliferation and apoptosis [3,4,5]. ZKSCAN3 has been reported as a novel driver of colorectal cancer progression [7] and to promote prostate cancer cell migration [8]. These results suggest that ZKSCAN3 modulates the expression of genes favoring cancer progression
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