Abstract
The aim is to investigate the effects of Zhuang Jin Xu Gu Decoction (ZJXG Decoction) on femoral fracture healing in rats. Femur fractures were generated in fifty male adult Wistar rats by cutting femur transversely at middle point. ZJXG Decoction was administered orally after surgery for 7-14 d. Radiological evaluation or X-ray imaging analysis indicated that the fibrous callus tissue at the femoral fracture-end increased and the fracture line became fuzzy at 7-14 d following treatment with ZJXG Decoction. Hematoxtlin-Eosin (HE) staining showed that the fibrous-granular tissue at the fracture-end changed gradually to fibrous, cartilaginous and osseous callus tissues. immunohistochemical staining and Enzyme Linked Immunobsorbent Assay (ELISA) results showed that BMP-7 in the fibroblasts and osteoblasts of callus and its serum level increased significantly 7-14 d following treatment with ZJXG Decoction. It is concluded that ZJXG Decoction could enhance the fracture healing by up-regulating the expression of BMP-7 in fibroblasts and osteoblasts of callus in rats.
Highlights
The fracture healing is an extremely complicated process of skeletal reconstruction
Bone Morphogenetic Proteins (BMPs) induces cartilage and bone formation [3] and been used to treat fracture models in rodents [4,5,6], and some experimental and clinical reports illustrated the effect of BMP7 on fracture healing [7,8,9]
We aimed to elucidate if the effects of ZJXG Decoction in fracture repair was related to the expression of BMP-7
Summary
Many growth factors could promote osteoblast development, proliferation, differentiation and accelerate new bone formation in the process of fracture healing and remodeling [1]. Bone Morphogenetic Proteins (BMPs) and Neuropeptide Y (NPY) play important roles in bone fracture repair process [2]. Fracture fixation could cause serious trauma and mostly need the secondary operation. It increases the risk of infection and the rates of delayed union, and nonunion [10]. We aimed to elucidate if the effects of ZJXG Decoction in fracture repair was related to the expression of BMP-7
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