Abstract
Objectives To investigate the mechanism of the protective effect of Zishenwan on pyelonephritis rats. Methods In the rat model of pyelonephritis, protective effects of Zishenwan, the content of secretory immunoglobulin A (SIg A), and interleukins were detected by ELISA. The expressions of TLR4-NFκB pathway were detected by Western blot in renal and urinary tract mucosa. The protective effect and influence on TLR4-NFκB pathway of Zishenwan were studied. Results Zishenwan protected rats from pyelonephritis which related to the increase of SIgA, the regulation of interleukins, and the inhibition of TLR4-NFκB pathway. Serum containing Zishenwan can significantly decrease LPS-induced expression of TLR4, MyD88, and NFκB in vitro. And the inhibition of TLR4 signal by Zishenwan related to the degradation of TRAF3 and TRAF6. Conclusions Zishenwan protected rats from urinary tract infection by clearance of bacteria and decrease of tissue damage. 20S proteasomes mediated the degradation of TRAF3 which is important to the decrease of tissue damage from Zishenwan.
Highlights
Urinary tract infection (UTI) is a common infectious disease in the general population [1]
In Zishenwan group, infiltrations of inflammatory and bacteria number were decreased and they were similar to the Levofloxacin group (Figure 1(c), p
We hypothesized that factors other than direct antibiotic activity should contribute to the treatment effect of Zishenwan
Summary
Urinary tract infection (UTI) is a common infectious disease in the general population [1]. Current therapies for urinary tract infection are based on the administration of antibiotics [3]. When UTI affects patients with low immune function, antibiotics might be used much longer and more frequent [3]. In this state, fungal infection, drug resistance, and dysbacteriosis associate and bring many difficulties for the clinical treatment of urinary tract infection, and recurrent UTI induces pyelonephritis. As a reason, enhancing systemic immunity and mucosal immunity should be important to prevent and control urinary tract infection, especially the recurrent urinary tract infection [3]. Traditional Chinese medicines are widely used in the therapy of urinary tract infection, especially the recurrent urinary tract infection in Asia [4, 5]
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